Rorug04G0235900

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014462
Physical Location & Seq
Forward (+)
40170464 .. 40241952
71489 bp
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UTR
Exon/CDS
Intron
Rorug04G0235900.1

Sequence Viewer

Length: 687 bp
ATGTCAACTTCTTCAGCTGGAGAAGGAACTGTCCTTCATATTGGGGGCCTGAAGAGCTACGTCACTGGCTCAACTGAATCTAAGCTTGCAGTCCTTCTAGTCTCCAACGTTGTTGGATATGAAAACCCAAACTTAAGGAAACTTGCGGATAAAGTTGCTGCAGCTGGATTTTTTGTGGTGGTTCCTGATTTCTTTTATGGCGACCCTATGAATCTTGATGATCCTCAATTTGACCGTGAGTCATGGCATAAAGCCCATAGCGTGGAAAAGGCATACGAGGATGCCAAACCAGTGCTTGCTGCTCTAAAGAGTCAAGGTGTTTCTGCAATAGGAGCTGCAGGATTTTGCTGGGGAGGGGTTGTAGTGGCAAAACTTGCAAAATCTGATGACATTAAGGCTGCTGTGATATTGCATCCTGGCCGGTTAGCAGATGAAGATATACATGAGGTTAAGGTTCATACTGCAATATTGGGAGCTGAGATTGACAAAACTTCACCCCCAGAACAATTGAAACAATTTGGGGAGATTTTATCAGTAAAGTCTGAGTTTGATAGCTTTGTGAAAATATTTCCTGGAGTGGCTCATGGATGGACAATGAGGTACAATGCGGAGGATGAATCTGCAGTCAAGAGTGCTGAAGAGGCTCATTTGGACATGTTAAATTGGTTTACCAAGTATGTCAAGTGA

Protein Analysis

228

Amino Acids

24.69

Weight (kDa)

5.47

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 18 - 226 1.2e-29 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014462)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50850
fragaria_vesca FvH4_4g23110 FvH4_4g23110 FvH4_4g23110
malus_domestica MD13G1125300.v1.1 MD16G1126100.v1.1
prunus_persica Prupe.1G220200_v2.0.a1 Prupe.1G220200_v2.0.a1
pyrus_communis pycom13g10920
rosa_chinensis RchiOBHm_Chr4g0429431
rosa_laevigata RLG00000007072
rosa_multiflora Rmu_sc0016937.1_g000001
rosa_roxburghii Rroxscaffold_5G00371050
rosa_rugosa Rorug04G0235800 Rorug04G0235900
rosa_samantha Rh4AG291600
rosa_wichuraiana Rw4G025250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 262
AciI CCGC 2 cut(s) 146, 608
AclI AACGTT 1 cut(s) 108
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 71, 657
AfaI GTAC 1 cut(s) 602
AfiI CCNNNNNNNGG 1 cut(s) 262
AflII CTTAAG 1 cut(s) 133
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 1 cut(s) 511
AhdI GACNNNNNGTC 1 cut(s) 238
AjnI CCWGG 2 cut(s) 415, 571
AluBI AGCT 7 cut(s) 17, 57, 85, 164, 335, 476, 555
AluI AGCT 7 cut(s) 17, 57, 85, 164, 335, 476, 555
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 1 cut(s) 215
AoxI GGCC 2 cut(s) 46, 418
ApeKI GCWGC 5 cut(s) 158, 161, 299, 335, 398
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 486
BbvI GCAGC 5 cut(s) 145, 173, 286, 322, 385
BccI CCATC 1 cut(s) 582
BciT130I CCWGG 2 cut(s) 417, 573
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 3 cut(s) 159, 336, 621
BfrI CTTAAG 1 cut(s) 133
BisI GCNGC 5 cut(s) 159, 162, 300, 336, 399
BlsI GCNGC 5 cut(s) 160, 163, 301, 337, 400
Bme1390I CCNGG 2 cut(s) 417, 573
BmeRI GACNNNNNGTC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 47, 183
BmrFI CCNGG 2 cut(s) 417, 573
BmsI GCATC 2 cut(s) 271, 421
BpmI CTGGAG 2 cut(s) 39, 594
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 1 cut(s) 420
Bse1I ACTGG 2 cut(s) 70, 290
BseBI CCWGG 2 cut(s) 417, 573
BseGI GGATG 4 cut(s) 286, 412, 593, 619
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMII CTCAG 2 cut(s) 468, 534
BseNI ACTGG 2 cut(s) 70, 290
BseXI GCAGC 5 cut(s) 145, 173, 286, 322, 385
BseYI CCCAGC 1 cut(s) 348
BshFI GGCC 2 cut(s) 48, 420
BsiSI CCGG 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 2 cut(s) 48, 420
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 2 cut(s) 146, 608
BspANI GGCC 2 cut(s) 48, 420
BspCNI CTCAG 2 cut(s) 469, 535
BspLI GGNNCC 2 cut(s) 47, 183
BspMAI CTGCAG 3 cut(s) 163, 340, 625
BspPI GGATC 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 47
BspTI CTTAAG 1 cut(s) 133
BsrFI RCCGGY 1 cut(s) 420
BsrI ACTGG 2 cut(s) 70, 290
BssAI RCCGGY 1 cut(s) 420
BssMI GATC 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 417, 573
Bst4CI ACNGT 2 cut(s) 31, 236
Bst6I CTCTTC 2 cut(s) 47, 633
BstAFI CTTAAG 1 cut(s) 133
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 87, 297
BstDEI CTNAG 3 cut(s) 81, 477, 543
BstF5I GGATG 4 cut(s) 286, 412, 593, 619
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 4 cut(s) 54, 332, 374, 641
BstNI CCWGG 2 cut(s) 417, 573
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 415, 571
BstSFI CTRYAG 3 cut(s) 159, 336, 621
BstV1I GCAGC 5 cut(s) 145, 173, 286, 322, 385
BsuRI GGCC 2 cut(s) 48, 420
BtsCI GGATG 4 cut(s) 286, 412, 593, 619
BtsIMutI CAGTG 2 cut(s) 63, 297
Cac8I GCNNGC 2 cut(s) 87, 297
Cfr10I RCCGGY 1 cut(s) 420
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 601
CviAII CATG 4 cut(s) 243, 443, 584, 655
CviQI GTAC 1 cut(s) 601
DdeI CTNAG 3 cut(s) 81, 477, 543
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 47, 633
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 2 cut(s) 47, 633
Eco57I CTGAAG 2 cut(s) 71, 657
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 415, 571
FaeI CATG 4 cut(s) 246, 446, 587, 658
FatI CATG 4 cut(s) 242, 442, 583, 654
Fnu4HI GCNGC 5 cut(s) 159, 162, 300, 336, 399
FokI GGATG 4 cut(s) 293, 399, 600, 626
Fsp4HI GCNGC 5 cut(s) 159, 162, 300, 336, 399
FspBI CTAG 1 cut(s) 98
GluI GCNGC 5 cut(s) 159, 162, 300, 336, 399
GsaI CCCAGC 1 cut(s) 352
GsuI CTGGAG 2 cut(s) 39, 594
HaeIII GGCC 2 cut(s) 48, 420
HapII CCGG 1 cut(s) 421
Hin1II CATG 4 cut(s) 246, 446, 587, 658
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 5 cut(s) 77, 211, 239, 310, 617
HpaII CCGG 1 cut(s) 421
HphI GGTGA 1 cut(s) 486
Hpy166II GTNNAC 2 cut(s) 6, 669
Hpy188I TCNGA 2 cut(s) 385, 544
Hpy188III TCNNGA 3 cut(s) 185, 215, 628
Hpy8I GTNNAC 2 cut(s) 6, 669
HpyAV CCTTC 3 cut(s) 17, 44, 104
HpyCH4III ACNGT 2 cut(s) 31, 236
HpyCH4IV ACGT 2 cut(s) 60, 108
HpyCH4V TGCA 8 cut(s) 89, 161, 326, 338, 377, 412, 464, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 332, 374, 641
HpyF3I CTNAG 3 cut(s) 81, 477, 543
HpySE526I ACGT 2 cut(s) 60, 108
Hsp92II CATG 4 cut(s) 246, 446, 587, 658
Kzo9I GATC 1 cut(s) 220
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 332, 473
Lsp1109I GCAGC 5 cut(s) 145, 173, 286, 322, 385
LweI GCATC 2 cut(s) 271, 421
MaeI CTAG 1 cut(s) 98
MaeII ACGT 2 cut(s) 60, 108
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 4 cut(s) 3, 64, 446, 650
MfeI CAATTG 1 cut(s) 506
MluCI AATT 4 cut(s) 227, 506, 515, 661
MlyI GAGTC 2 cut(s) 248, 319
MmeI TCCRAC 2 cut(s) 94, 129
MnlI CCTC 7 cut(s) 234, 271, 347, 439, 591, 604, 634
MseI TTAA 4 cut(s) 134, 393, 450, 659
MspA1I CMGCKG 2 cut(s) 17, 164
MspCI CTTAAG 1 cut(s) 133
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 417, 573
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 2 cut(s) 417, 573
MwoI GCNNNNNNNGC 4 cut(s) 54, 332, 374, 641
NdeII GATC 1 cut(s) 220
NlaIII CATG 4 cut(s) 246, 446, 587, 658
NlaIV GGNNCC 2 cut(s) 47, 183
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 1 cut(s) 658
PciI ACATGT 1 cut(s) 654
PciSI GCTCTTC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 77, 211, 617
PflMI CCANNNNNTGG 1 cut(s) 262
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 5 cut(s) 160, 163, 301, 337, 400
PleI GAGTC 2 cut(s) 247, 318
PpsI GAGTC 2 cut(s) 247, 318
PscI ACATGT 1 cut(s) 654
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 415, 571
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 2 cut(s) 415, 571
PspN4I GGNNCC 2 cut(s) 47, 183
PspPI GGNCC 1 cut(s) 46
PstI CTGCAG 3 cut(s) 163, 340, 625
PvuII CAGCTG 2 cut(s) 17, 164
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 4 cut(s) 134, 393, 450, 659
SatI GCNGC 5 cut(s) 159, 162, 300, 336, 399
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 2 cut(s) 248, 319
ScrFI CCNGG 2 cut(s) 417, 573
SfaNI GCATC 2 cut(s) 271, 421
SfcI CTRYAG 3 cut(s) 159, 336, 621
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
Sse9I AATT 4 cut(s) 227, 506, 515, 661
SsiI CCGC 2 cut(s) 146, 608
SspI AATATT 2 cut(s) 468, 567
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 415, 571
TaaI ACNGT 2 cut(s) 31, 236
TaiI ACGT 2 cut(s) 63, 111
TasI AATT 4 cut(s) 227, 506, 515, 661
TfiI GAWTC 3 cut(s) 77, 211, 617
Tru1I TTAA 4 cut(s) 134, 393, 450, 659
Tru9I TTAA 4 cut(s) 134, 393, 450, 659
TscAI CASTG 2 cut(s) 70, 297
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 5 cut(s) 158, 161, 299, 335, 398
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 6 cut(s) 26, 135, 224, 446, 447, 630
TspRI CASTG 2 cut(s) 70, 297
Van91I CCANNNNNTGG 1 cut(s) 262
Vha464I CTTAAG 1 cut(s) 133
XceI RCATGY 1 cut(s) 658
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.