Rorug04G0264200

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002813
Physical Location & Seq
Reverse (-)
43569400 .. 43574386
4987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0264200.1

Sequence Viewer

Length: 2178 bp
ATGGGTAGCAACGCGAAGGGAAGGACTGTGCTTGAGGTTGGAGCTGATGGGGTGGCGCTCATCACCATCATCAACCCTCCTGTCAATTCCCTCTCCTTTGATGTGTTATTTGGCTTGAAAGAGAGCTATGAGGAGGCTCTGAGGAGAGAAGATGTCAAGGCAATTGTCGTTACAGGTGCAAGAGGGAAATTCTCTGGCGGATTTGATATTACTGCTTTTGGTGGACTTCAAGGAGGGCAGACAGCGGAGCCGAAGCCTGGTTATATATCTGTGGAGGTTATCACTAACACTGTAGAAGGTGCTAGGAAACCTTCAGTTGCTGCCATTGATGGCCTAGCATTGGGGGGAGGATTAGAGGTTGCGATGGCTTGCCATGCTCGAATATCAACTCCGACTGCCCAATTAGGCTTGCCAGAACTTCAGCTTGGACTCATCCCTGGATTTGGAGGAACGCAACGGCTTCCGCGTCTTGTTGGAATTTCAAAGGCACTTGAAATGATGCTGACTTCAAAACCGGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTTGATGCTATAGTTTCACCTGATGAGTTGGTAAGCACTGCACGTCGGTGGGCCTTGGATATCTTGGAGCGTCGGAGACCATGGGTTGCTAGTCTTCATAAGACTGACAAGTTAGAACCCCTTGGCGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTACAGAGGCAGGCTCCCAATCTCAAACATCCATTGGTTTGCATTGATGTTGTTGAAGAGGGTATAGCTTCTGGTGGCCGGGCTGGACTTTGGAAGGAAGCTCAAGAATTTCAAGGACTTCTGCGTGCTGACACTTGCAAGAGCTTGGTCCACGTCTTCTTTGCTCAACGTGGAACATCAAAGGTACCTGGGGTTACTGATCGAGGTTTGGTGCCTAGACGAGTGACTAAGGTTGCTGTCATTGGTGGAGGCTTGATGGGCTCTGGAATAACTACAGCATTGATTCTTAGTAATTATCCAGTGATCCTGAAAGAAGTGAATGAGAAGTTCTTGCAGGCTGGGATTGGTAGAGTCAAAGCCAATCTGCAGAGCCGCGTCAAAAAAGGGAAAATGACTGAAGAGAAGTTTGAGAAGACGCTCTCTCTTCTTAAAGGTACCCTGGACTATGAAAGCTTTAGAGATGTTGACATAGTCATAGAGGCAGTTATTGAGAAGGTTTCTTTAAAGCAACAAATTTTTGCTGACCTTGAAAAATATTGCCCACAACACTGCATACTTGCTAGTAACACTTCCACAATTGACTTGAACCTTATTGGAGAGAAGACCAAATCCCATGATCGAATTATTGGAGCCCATTTCTTTAGTCCAGCACATGTCATGCCACTTTTGGAAATTGTTCGCACTAATCAGACATCTCCACAAGTTATTGTTGATTTGCTAGAAGTTGGGAAGAAGATAAAGAAAACCCCAGTTGTTGTTGGGAACTGCACTGGATTTGCTGTTAACAGAATGTTCTTTCCTTATACCCAAGCTGGTCTTCTGCTTATTGAGCGTGGTGCAGATGTATATCAGATTGACAGGGCAATCACCAAATTTGGAATGCCCATGGGCCCATTCAGATTGGCTGATCTGGTTGGCTTTGGTGTGGCGATTGCAACTGGCATGCAATTTATTGAGAATTTTCCTGAGCGGACATATAAGTCGATGCTTATTCCTATGATGCAGGAGGATGGGAGAGCAGGTGAAACTACAAAAAGAGGGTTTTATGTTTATGATGATAAACGCAAAGCTAAGCCAGATCCTGAATTGAAGAAGTACATTGAGAAGTCAAGAAGCATTTCTGGTCTTTCAATAGATGCCAAGCTAGTGAAGTTACCAGAAAAGGACATTGTGGAAATGATCTTTTTCCCTGTTGTGAATGAGGCCTGCAGAGTGTATGCTGAAGGTATTGCTGTAAAAGCTGCTGACCTTGATATTGCTTCAGTCATGGGCATGGGGTTTCCACCTTACAGGGGAGGTATCATGTTCTGGGCCGATTTACTTGGATCCAAATACATATATTCAAGACTGGAGCAATGGTCAAACATTTATGGTGAATTTTTCAAGCCTTGTGCCTATTTAGCTGAAAGGGCTGCACAGGGAGCTCCTCTGAGCTGTCCTTTGAAGCAATCAAAATCTCGGTTATAA

Protein Analysis

725

Amino Acids

79.2

Weight (kDa)

9.06

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 16 - 216 3e-40 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 28 - 196 7.8e-23 Enoyl-CoA hydratase/isomerase
3HCDH_N PF02737 314 - 492 9.1e-62 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH PF00725 495 - 588 3e-21 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2176
AarI CACCTGC 1 cut(s) 1721
AasI GACNNNNNNGTC 1 cut(s) 1690
Acc36I ACCTGC 1 cut(s) 1721
Acc65I GGTACC 2 cut(s) 895, 1145
AccB1I GGYRCC 3 cut(s) 895, 922, 1145
AccBSI CCGCTC 1 cut(s) 1681
AccII CGCG 3 cut(s) 14, 466, 1086
AciI CCGC 5 cut(s) 198, 245, 464, 1084, 1681
AclWI GGATC 4 cut(s) 1009, 1784, 2031, 2044
AcoI YGGCCR 1 cut(s) 787
AcsI RAATTY 8 cut(s) 188, 477, 695, 818, 1224, 1583, 1669, 2087
AcuI CTGAAG 5 cut(s) 297, 404, 1128, 1953, 1956
AfaI GTAC 4 cut(s) 714, 897, 1147, 1808
AfiI CCNNNNNNNGG 5 cut(s) 257, 340, 443, 521, 2003
AflIII ACRYGT 1 cut(s) 1363
AgeI ACCGGT 1 cut(s) 514
AjiI CACGTC 2 cut(s) 593, 865
AjnI CCWGG 4 cut(s) 256, 436, 898, 1149
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 1512, 1544
AleI CACNNNNGTG 1 cut(s) 595
Alw21I GWGCWC 2 cut(s) 709, 2137
Alw26I GTCTC 1 cut(s) 619
AlwI GGATC 4 cut(s) 1009, 1784, 2031, 2044
AlwNI CAGNNNCTG 2 cut(s) 320, 1793
AoxI GGCC 7 cut(s) 331, 544, 600, 787, 1600, 1914, 2022
ApaI GGGCCC 1 cut(s) 1604
ApeKI GCWGC 3 cut(s) 320, 1952, 2123
ApoI RAATTY 8 cut(s) 188, 477, 695, 818, 1224, 1583, 1669, 2087
AsiGI ACCGGT 1 cut(s) 514
Asp700I GAANNNNTTC 2 cut(s) 1386, 1828
Asp718I GGTACC 2 cut(s) 895, 1145
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 5 cut(s) 600, 859, 1600, 1601, 2022
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 5 cut(s) 55, 558, 1570, 1745, 2096
AvaII GGWCC 1 cut(s) 859
BaeGI GKGCMC 1 cut(s) 1604
BamHI GGATCC 1 cut(s) 2036
BanI GGYRCC 3 cut(s) 895, 922, 1145
BanII GRGCYC 5 cut(s) 709, 974, 1345, 1604, 2137
BbsI GAAGAC 5 cut(s) 635, 859, 1130, 1319, 1520
Bbv12I GWGCWC 2 cut(s) 709, 2137
BbvI GCAGC 3 cut(s) 307, 1939, 2110
BccI CCATC 6 cut(s) 41, 74, 323, 358, 961, 1715
BceAI ACGGC 1 cut(s) 473
BciT130I CCWGG 4 cut(s) 258, 438, 900, 1151
BcnI CCSGG 1 cut(s) 791
BcoDI GTCTC 1 cut(s) 619
BfaI CTAG 8 cut(s) 303, 335, 639, 681, 927, 1272, 1430, 1856
BfmI CTRYAG 5 cut(s) 291, 558, 984, 1076, 1918
BfoI RGCGCY 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 1721
BisI GCNGC 4 cut(s) 321, 1084, 1953, 2124
BlpI GCTNAGC 1 cut(s) 1782
BlsI GCNGC 4 cut(s) 322, 1085, 1954, 2125
Bme1390I CCNGG 5 cut(s) 258, 438, 791, 900, 1151
Bme18I GGWCC 1 cut(s) 859
BmgBI CACGTC 2 cut(s) 593, 865
BmgT120I GGNCC 5 cut(s) 600, 859, 1600, 1601, 2022
BmiI GGNNCC 8 cut(s) 249, 726, 897, 924, 1147, 1342, 1602, 2038
BmrFI CCNGG 5 cut(s) 258, 438, 791, 900, 1151
BmrI ACTGGG 1 cut(s) 1454
BmsI GCATC 5 cut(s) 489, 544, 1686, 1701, 1837
BmuI ACTGGG 1 cut(s) 1454
BpiI GAAGAC 5 cut(s) 635, 859, 1130, 1319, 1520
BplI GAGNNNNNCTC 2 cut(s) 709, 741
BpmI CTGGAG 1 cut(s) 2081
Bpu10I CCTNAGC 1 cut(s) 1677
Bpu1102I GCTNAGC 1 cut(s) 1782
BpuEI CTTGAG 3 cut(s) 53, 693, 798
BpuMI CCSGG 1 cut(s) 791
BsaBI GATNNNNATC 1 cut(s) 1557
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 7 cut(s) 436, 603, 629, 670, 899, 1149, 1596
BsaWI WCCGGW 1 cut(s) 514
BsaXI ACNNNNNCTCC 4 cut(s) 1717, 1747, 2054, 2084
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 340, 443, 521, 2003
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 5 cut(s) 1010, 1460, 1486, 1654, 2064
Bse3DI GCAATG 1 cut(s) 2072
Bse8I GATNNNNATC 1 cut(s) 1557
BseBI CCWGG 4 cut(s) 258, 438, 900, 1151
BseDI CCNNGG 7 cut(s) 436, 603, 629, 670, 899, 1149, 1596
BseGI GGATG 3 cut(s) 432, 739, 1726
BseJI GATNNNNATC 1 cut(s) 1557
BseLI CCNNNNNNNGG 5 cut(s) 257, 340, 443, 521, 2003
BseMI GCAATG 1 cut(s) 2072
BseMII CTCAG 3 cut(s) 131, 1668, 2132
BseNI ACTGG 5 cut(s) 1010, 1460, 1486, 1654, 2064
BseRI GAGGAG 3 cut(s) 146, 157, 2127
BseSI GKGCMC 1 cut(s) 1604
BseXI GCAGC 3 cut(s) 307, 1939, 2110
BseYI CCCAGC 1 cut(s) 1049
BsgI GTGCAG 4 cut(s) 573, 1462, 1569, 2109
Bsh1236I CGCG 3 cut(s) 14, 466, 1086
BshFI GGCC 7 cut(s) 333, 546, 602, 789, 1602, 1916, 2024
BshNI GGYRCC 3 cut(s) 895, 922, 1145
BshTI ACCGGT 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 709, 2137
BsiSI CCGG 2 cut(s) 515, 790
BslI CCNNNNNNNGG 5 cut(s) 257, 340, 443, 521, 2003
BsmAI GTCTC 1 cut(s) 619
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 7 cut(s) 333, 546, 602, 789, 1602, 1916, 2024
Bso31I GGTCTC 1 cut(s) 619
Bsp120I GGGCCC 1 cut(s) 1600
Bsp1286I GDGCHC 5 cut(s) 709, 974, 1345, 1604, 2137
Bsp143I GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
Bsp1720I GCTNAGC 1 cut(s) 1782
Bsp19I CCATGG 2 cut(s) 629, 1596
BspACI CCGC 5 cut(s) 198, 245, 464, 1084, 1681
BspANI GGCC 7 cut(s) 333, 546, 602, 789, 1602, 1916, 2024
BspCNI CTCAG 3 cut(s) 132, 1669, 2133
BspFNI CGCG 3 cut(s) 14, 466, 1086
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 8 cut(s) 249, 726, 897, 924, 1147, 1342, 1602, 2038
BspMAI CTGCAG 2 cut(s) 1080, 1922
BspMI ACCTGC 1 cut(s) 1721
BspPI GGATC 4 cut(s) 1009, 1784, 2031, 2044
BspT107I GGYRCC 3 cut(s) 895, 922, 1145
BspTNI GGTCTC 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 1681
BsrDI GCAATG 1 cut(s) 2072
BsrFI RCCGGY 1 cut(s) 514
BsrI ACTGG 5 cut(s) 1010, 1460, 1486, 1654, 2064
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 7 cut(s) 436, 603, 629, 670, 899, 1149, 1596
BssMI GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
BssT1I CCWWGG 4 cut(s) 603, 629, 670, 1596
Bst2UI CCWGG 4 cut(s) 258, 438, 900, 1151
Bst4CI ACNGT 2 cut(s) 28, 292
Bst6I CTCTTC 4 cut(s) 522, 762, 1104, 1140
BstC8I GCNNGC 7 cut(s) 370, 410, 723, 837, 1047, 1655, 1918
BstDEI CTNAG 6 cut(s) 140, 939, 998, 1677, 1782, 2141
BstDSI CCRYGG 2 cut(s) 629, 1596
BstF5I GGATG 3 cut(s) 432, 739, 1726
BstFNI CGCG 3 cut(s) 14, 466, 1086
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 7 cut(s) 913, 1017, 1330, 1621, 1792, 1893, 2039
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
BstMWI GCNNNNNNNGC 7 cut(s) 374, 969, 1540, 1949, 2111, 2120, 2132
BstNI CCWGG 4 cut(s) 258, 438, 900, 1151
BstNSI RCATGY 2 cut(s) 1367, 1657
BstSCI CCNGG 5 cut(s) 256, 436, 789, 898, 1149
BstSFI CTRYAG 5 cut(s) 291, 558, 984, 1076, 1918
BstSLI GKGCMC 1 cut(s) 1604
BstUI CGCG 3 cut(s) 14, 466, 1086
BstV1I GCAGC 3 cut(s) 307, 1939, 2110
BstV2I GAAGAC 5 cut(s) 635, 859, 1130, 1319, 1520
BstX2I RGATCY 2 cut(s) 1789, 2036
BstYI RGATCY 2 cut(s) 1789, 2036
BsuRI GGCC 7 cut(s) 333, 546, 602, 789, 1602, 1916, 2024
BtgI CCRYGG 2 cut(s) 629, 1596
BtgZI GCGATG 1 cut(s) 377
BtrI CACGTC 2 cut(s) 593, 865
BtsCI GGATG 3 cut(s) 432, 739, 1726
BtsI GCAGTG 2 cut(s) 585, 1258
BtsIMutI CAGTG 5 cut(s) 288, 585, 1017, 1258, 1479
BveI ACCTGC 1 cut(s) 1721
Cac8I GCNNGC 7 cut(s) 370, 410, 723, 837, 1047, 1655, 1918
CaiI CAGNNNCTG 2 cut(s) 320, 1793
CciI TCATGA 1 cut(s) 535
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 5 cut(s) 600, 859, 1600, 1601, 2022
CseI GACGC 4 cut(s) 455, 608, 1075, 1135
Csp6I GTAC 4 cut(s) 713, 896, 1146, 1807
CspAI ACCGGT 1 cut(s) 514
CviQI GTAC 4 cut(s) 713, 896, 1146, 1807
DdeI CTNAG 6 cut(s) 140, 939, 998, 1677, 1782, 2141
DpnI GATC 7 cut(s) 912, 1016, 1329, 1620, 1791, 1892, 2038
DpnII GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
DraI TTTAAA 1 cut(s) 1215
DrdI GACNNNNNNGTC 1 cut(s) 1690
DseDI GACNNNNNNGTC 1 cut(s) 1690
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 4 cut(s) 522, 762, 1104, 1140
EarI CTCTTC 4 cut(s) 522, 762, 1104, 1140
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 2 cut(s) 707, 2135
Eco130I CCWWGG 4 cut(s) 603, 629, 670, 1596
Eco147I AGGCCT 2 cut(s) 546, 1916
Eco24I GRGCYC 5 cut(s) 709, 974, 1345, 1604, 2137
Eco31I GGTCTC 1 cut(s) 619
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 859
Eco53kI GAGCTC 2 cut(s) 707, 2135
Eco57I CTGAAG 5 cut(s) 297, 404, 1128, 1953, 1956
EcoICRI GAGCTC 2 cut(s) 707, 2135
EcoRII CCWGG 4 cut(s) 256, 436, 898, 1149
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 4 cut(s) 603, 629, 670, 1596
EcoT38I GRGCYC 5 cut(s) 709, 974, 1345, 1604, 2137
ErhI CCWWGG 4 cut(s) 603, 629, 670, 1596
FalI AAGNNNNNCTT 2 cut(s) 1512, 1544
Fnu4HI GCNGC 4 cut(s) 321, 1084, 1953, 2124
FokI GGATG 3 cut(s) 419, 726, 1733
FriOI GRGCYC 5 cut(s) 709, 974, 1345, 1604, 2137
Fsp4HI GCNGC 4 cut(s) 321, 1084, 1953, 2124
FspBI CTAG 8 cut(s) 303, 335, 639, 681, 927, 1272, 1430, 1856
GlaI GCGC 1 cut(s) 57
GluI GCNGC 4 cut(s) 321, 1084, 1953, 2124
GsaI CCCAGC 1 cut(s) 1053
GsuI CTGGAG 1 cut(s) 2081
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 7 cut(s) 333, 546, 602, 789, 1602, 1916, 2024
HapII CCGG 2 cut(s) 515, 790
HgaI GACGC 4 cut(s) 455, 608, 1075, 1135
HhaI GCGC 1 cut(s) 58
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 2 cut(s) 1177, 1495
HindII GTYRAC 2 cut(s) 1177, 1495
HindIII AAGCTT 1 cut(s) 1162
HinfI GANTC 3 cut(s) 429, 994, 1062
HpaI GTTAAC 1 cut(s) 1495
HpaII CCGG 2 cut(s) 515, 790
HphI GGTGA 5 cut(s) 55, 558, 1570, 1745, 2096
Hpy166II GTNNAC 4 cut(s) 224, 862, 1177, 1495
Hpy188I TCNGA 7 cut(s) 141, 393, 624, 1401, 1563, 1610, 2142
Hpy188III TCNNGA 8 cut(s) 536, 815, 975, 1018, 1676, 1793, 1821, 2055
Hpy8I GTNNAC 4 cut(s) 224, 862, 1177, 1495
Hpy99I CGWCG 2 cut(s) 597, 624
HpyAV CCTTC 7 cut(s) 10, 15, 290, 321, 799, 1198, 1928
HpyCH4III ACNGT 2 cut(s) 28, 292
HpyCH4IV ACGT 3 cut(s) 592, 864, 880
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 969, 1540, 1949, 2111, 2120, 2132
HpyF3I CTNAG 6 cut(s) 140, 939, 998, 1677, 1782, 2141
HpySE526I ACGT 3 cut(s) 592, 864, 880
HspAI GCGC 1 cut(s) 56
KpnI GGTACC 2 cut(s) 899, 1149
KspAI GTTAAC 1 cut(s) 1495
Kzo9I GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
LmnI GCTCC 8 cut(s) 41, 247, 616, 730, 1340, 2062, 2132, 2140
Lsp1109I GCAGC 3 cut(s) 307, 1939, 2110
LweI GCATC 5 cut(s) 489, 544, 1686, 1701, 1837
MaeI CTAG 8 cut(s) 303, 335, 639, 681, 927, 1272, 1430, 1856
MaeII ACGT 3 cut(s) 592, 864, 880
MaeIII GTNAC 5 cut(s) 169, 904, 934, 1274, 1863
MalI GATC 7 cut(s) 912, 1016, 1329, 1620, 1791, 1892, 2038
MbiI CCGCTC 1 cut(s) 1681
MboI GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
MfeI CAATTG 2 cut(s) 162, 1287
MflI RGATCY 2 cut(s) 1789, 2036
MhlI GDGCHC 5 cut(s) 709, 974, 1345, 1604, 2137
MlyI GAGTC 2 cut(s) 423, 1071
MmeI TCCRAC 4 cut(s) 19, 416, 454, 602
MroXI GAANNNNTTC 2 cut(s) 1386, 1828
MseI TTAA 5 cut(s) 519, 693, 1140, 1214, 1494
MslI CAYNNNNRTG 2 cut(s) 595, 1982
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 515, 790
MspR9I CCNGG 5 cut(s) 258, 438, 791, 900, 1151
MunI CAATTG 2 cut(s) 162, 1287
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 4 cut(s) 258, 438, 900, 1151
MvnI CGCG 3 cut(s) 14, 466, 1086
MwoI GCNNNNNNNGC 7 cut(s) 374, 969, 1540, 1949, 2111, 2120, 2132
NciI CCSGG 1 cut(s) 791
NcoI CCATGG 2 cut(s) 629, 1596
NdeII GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
NlaIV GGNNCC 8 cut(s) 249, 726, 897, 924, 1147, 1342, 1602, 2038
NmuCI GTSAC 1 cut(s) 934
NspI RCATGY 2 cut(s) 1367, 1657
OliI CACNNNNGTG 1 cut(s) 595
PaeI GCATGC 1 cut(s) 1657
PagI TCATGA 1 cut(s) 535
PaqCI CACCTGC 1 cut(s) 1721
PceI AGGCCT 2 cut(s) 546, 1916
PciI ACATGT 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PctI GAATGC 1 cut(s) 1596
PdmI GAANNNNTTC 2 cut(s) 1386, 1828
PfeI GAWTC 1 cut(s) 994
PflFI GACNNNGTC 1 cut(s) 1181
PinAI ACCGGT 1 cut(s) 514
PkrI GCNGC 4 cut(s) 322, 1085, 1954, 2125
PleI GAGTC 2 cut(s) 423, 1070
PpsI GAGTC 2 cut(s) 423, 1070
PscI ACATGT 1 cut(s) 1363
PsiI TTATAA 1 cut(s) 2176
Psp124BI GAGCTC 2 cut(s) 709, 2137
Psp6I CCWGG 4 cut(s) 256, 436, 898, 1149
PspFI CCCAGC 1 cut(s) 1049
PspGI CCWGG 4 cut(s) 256, 436, 898, 1149
PspN4I GGNNCC 8 cut(s) 249, 726, 897, 924, 1147, 1342, 1602, 2038
PspOMI GGGCCC 1 cut(s) 1600
PspPI GGNCC 5 cut(s) 600, 859, 1600, 1601, 2022
PstI CTGCAG 2 cut(s) 1080, 1922
PstNI CAGNNNCTG 2 cut(s) 320, 1793
PsuI RGATCY 2 cut(s) 1789, 2036
PsyI GACNNNGTC 1 cut(s) 1181
RsaI GTAC 4 cut(s) 714, 897, 1147, 1808
RsaNI GTAC 4 cut(s) 713, 896, 1146, 1807
RseI CAYNNNNRTG 2 cut(s) 595, 1982
SacI GAGCTC 2 cut(s) 709, 2137
SaqAI TTAA 5 cut(s) 519, 693, 1140, 1214, 1494
SatI GCNGC 4 cut(s) 321, 1084, 1953, 2124
Sau3AI GATC 7 cut(s) 910, 1014, 1327, 1618, 1789, 1890, 2036
Sau96I GGNCC 5 cut(s) 600, 859, 1600, 1601, 2022
SchI GAGTC 2 cut(s) 423, 1071
ScrFI CCNGG 5 cut(s) 258, 438, 791, 900, 1151
SduI GDGCHC 5 cut(s) 709, 974, 1345, 1604, 2137
SfaNI GCATC 5 cut(s) 489, 544, 1686, 1701, 1837
SfcI CTRYAG 5 cut(s) 291, 558, 984, 1076, 1918
SinI GGWCC 1 cut(s) 859
SmiMI CAYNNNNRTG 2 cut(s) 595, 1982
SmlI CTYRAG 3 cut(s) 32, 708, 813
SmoI CTYRAG 3 cut(s) 32, 708, 813
SphI GCATGC 1 cut(s) 1657
SseBI AGGCCT 2 cut(s) 546, 1916
SsiI CCGC 5 cut(s) 198, 245, 464, 1084, 1681
SspI AATATT 1 cut(s) 1247
SspMI CTAG 8 cut(s) 303, 335, 639, 681, 927, 1272, 1430, 1856
SstI GAGCTC 2 cut(s) 709, 2137
StuI AGGCCT 2 cut(s) 546, 1916
StyD4I CCNGG 5 cut(s) 256, 436, 789, 898, 1149
StyI CCWWGG 4 cut(s) 603, 629, 670, 1596
TaaI ACNGT 2 cut(s) 28, 292
TaiI ACGT 3 cut(s) 595, 867, 883
TaqI TCGA 4 cut(s) 379, 913, 1330, 1694
TatI WGTACW 2 cut(s) 712, 1806
TauI GCSGC 1 cut(s) 1086
TfiI GAWTC 1 cut(s) 994
Tru1I TTAA 5 cut(s) 519, 693, 1140, 1214, 1494
Tru9I TTAA 5 cut(s) 519, 693, 1140, 1214, 1494
TscAI CASTG 5 cut(s) 295, 592, 1017, 1265, 1486
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 3 cut(s) 320, 1952, 2123
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 2 cut(s) 635, 1173
TspRI CASTG 5 cut(s) 295, 592, 1017, 1265, 1486
Tth111I GACNNNGTC 1 cut(s) 1181
VpaK11BI GGWCC 1 cut(s) 859
XapI RAATTY 8 cut(s) 188, 477, 695, 818, 1224, 1583, 1669, 2087
XceI RCATGY 2 cut(s) 1367, 1657
XmnI GAANNNNTTC 2 cut(s) 1386, 1828
XspI CTAG 8 cut(s) 303, 335, 639, 681, 927, 1272, 1430, 1856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.