Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0008235

Physical Location & Seq

Genomic Coordinates Forward (+)

47610620 .. 47618048

Gene Structure 7429 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0301200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4314 bp

ATGGAGCTAACACAAGTTAAGAGAGACGTCCACTCATCGTTTCGACACCAAGCTTCGAGCTTCGGTGCTGGTTCAGACTCAATCAAAGAGGAGGATGAGGAAGCTCAGCTGCAGTGGGCTGCCATTGAGAGATTGCCTACATTCAGAAGGCTGAGGACCTCACTTTTCGACCTCGGTGATAGCGAAAATGGAAAAACCAGTGGCGAAGAGTGTGCAGGGAAGAAGGTGGTTGATGTTACCAAGTTGGGAGCAGATGAGAGGCATCTTTTCATTGAGAAGATCATCAAGCACATTGAGCATGATAATCTGAGGCTGCTACAAAAAATGAGAGAAAGGATAGACAGAGTGAATGTGAAATTGCCAACTGTGGAGGTGAGGTACAAGAGTTTGTGTGTGGAAGCCGAGTGTGAAGTAGTACAAGGAAAGCCGCTCCCGACGCTGTGGAACTCTCTTCTAAGCTTGTTATCAGTTTTTCGAAAGGTAATGTGGTGCAAGTCTCAAGATGCAAAGATTAGCGTTCTCACAGATGTCAGTGGCGTCATCAAACCTTCAAGGATCACTCTCCTTCTTGGTCCCCCGGGGTGTGGTAAAACCACCTTCTTATTGGCACTTGCCGGAAAACTGGATAAATCCCTGAAGGTTGCAGGGGAGATTTCATACAATGGTTACAAGCTAGATGAGTTTGTTCCTCAAAAAACTTCAGCGTACATAAGCCAACACGACCTTCACGTACCTGAGATGACTGTAAGAGAAACAATCGAGTTTTCAGCCCGCTGTCAAGGTGTTGGAAGCAGAGCTGATATAATGCTAGAGGTTAGCAGGAGAGAAAAGGAAGCAGGAATTGTTCCTGATCCAGATATTGACACCTACATGAAGGCAATCTCAGTTGAAGGACAAAAGAAAAACCTCCAAACTGACTATGTTTTGAAGATGCTAGGACTAGATATCTGCAGTGACACAATGGTTGGTGATGCATTACAAAGAGGTATTTCGGGTGGCCAAAAGAAAAGGCTGACAACAGGAGAGATGATTGTCGGTCCCACAAAAGCGCTATTTATGGATGAAATATCAACTGGATTAGACAGCTCTACGACTTTTCAGATAGTCACATATCTACAGCAGTTGGTGCACATAACAGATACAACTGCATTGGTGTCACTTCTACAACCAGCACCTGAGACATTCGATTTATTTGATGATGTGATACTAATGGCAGAGGGGAAGATAGTTTACCATGGTCCTCGGAGTCATGCACTTCAGTTTTTCGAAGATTGTGGTTTTAAGTGCCCACCAAGGAAGGGTGCTGCAGATTATCTTCAGGAGGTAATCTCAAAGAAGGATCAAGCACAATACTGGAAGCATGATAACATTCCCTATGATTATGTTTCTGTGGACAAATTCTCTCAGCTTTTCAGAGCAAGTTACTTGGGAAAGAAGTTAACTGATGAGCTCTCAAAACCATATGAGAAATCACCGGGCCAGGATAAGGCTTTATCATTTAGCATATACTCTGTCAGAAAATGGGAATTGTTCAAAGCTTGCATGGGTAGAGAGCTGCTTCTTATGAAACGAAATTCCTATGTTTATGTATTCAAAACAGCGCAGCTTGTCATCACTGCACTTTTGACAACGACAGTATTCATACGTACTCAGATGGATGTGGATTTGACAAGTGCAAATTATTTTATGGGTGCATTGTTCTATACACTTGTGAGACTTATGACCAATGGAGTTGCAGAACTGTCCTTGACTATCACTAGACTACCAGTGGTTGATAAGCAAAGAGCCTTCTATCTTTATCCAGCATGGTCCTATTCAATTCCAGCCTCTCTCCTGAAGATTCCATTTTCACTGAGTGATGCGGTGTTGTGGACAGCAATAACTTACTATGGTGTTGGGTATAGCCCTGAAATAAAAAGGTTCTTCTGCCAGCTCCTTGTGCTGTTTGCTCTGCATCTTGCATCAACATCCATGTGTCGTTTGATTGCAGTAATTTTCCGATCCATGGTTGTTGCAACAACTATTGGTACTTTAATCTTGGTACTAATGTTTGTATTTGGAGGCTTCATTTTGCCACCACCCTCATTACCTGTTTGGTTGAAGTGGGGATTCTGGTGTTCTCCTATGACTTATGGGGAAATAGCTGCAAGTCTGAATGAATTCCGTGCTCCTCGATGGCAAAAGGTTTCAAAGGGAAACACAACCTTGGGGAATGAAATTCTAACCAGTCATGGTTTGAACTTTGGTAGCTACTTCTACTGGATATCAGTAGGGGCTTTATTCGGGTTCACAGCTATTTTCGATCTTGGATTTGTTTTAGCCTTAACTTACTTAAAATCTCCAAAGATGTCTCGTGCTATTATTTCGAAAAAAAGATTATCTCAACTCAAAGGACAAGATTCTTGGAACAAGACCGGTGCAAAGTCAGATAATGTATCAACTCCAGCTGATTTTTCCCAAACCATGGAAGAAACAGGGAAGATGGTTCTGCCATTTGAGCCGCTGACAATCTCATTTAAGGATGTGCAGTACAGTGTTGACATCCCACCGGAAATGAAAAGGCACGGTTTCGATCAGAAAAAACTTCAGCTGCTTAAAGATATTACAGGAGCATTTAGGCCGGGAGTTCTTACAGCATTGATGGGAGTCAGTGGGGCTGGGAAAACAACTCTCATGGATGTTCTTTCGGGAAGGAAAACTGGAGGCACTATTGAAGGAGATATAAGAATAGGAGGCTATCCCAAAGTCCAGAAGACATTCGCTAGAATATCAGGTTACTGTGAACAATTTGATATACATTCTCCACACATCACAGTAGGAGAGTCGGTTATGTTTTCAGCTTGGTTGAGGTTGGCACCCGAAATTGATCAAGACACAAAAGCTAAATTTGTAGAAGAAGTTATTGAAACAATTGAATTGGAGGATATAAGAGATTCTTTAGTTGGCATTCCTGGCCAAAGTGGCCTATCAACCGAACAGCGCAAAAGGCTAACAATTGCAGTTGAGCTTGTTTCCAATCCATCAATAATATTTATGGATGAACCTACATCAGGACTAGATGCCAGAGCAGCGGCAATTGTCATGCGTGCTGTGAAGAATGTAGTTGCCACAGGAAGAACAACTATTTGCACAATCCACCAACCTAGCATTGATATCTTTGAGGCTTTTGATGAGTTAATTCTGATGAAAACGGGAGGGCAAATTATCTATTCTGGAAGTCTGGGCCATCATTCGAGTAATCTCATTGAATATTTTGAGGGTATTCCTGGTGTACCAAAGATCAAAGATAACTATAACCCCGCAACATGGATGTTAGAAGTTACTTCCTCTTCAGTAGAAGCAGAACTCGGTTTAGATTTTGCGAACACTTATAAAGAATCTACTCTGTATAAAGACACAGTTGAATTGGTGAGACAGATAAGTGAACCAAAGCCAGGTCCAAGAGCTTTGCAATTTTCCACCCAATTTCCACAAAATAGCTGGGTGCAGTTTAGGGCATGCTTATGGAAACAACACTTATCCTACTGGAGAAGTCCTGAATACAATCTAGCACGTTTCATGTTCATGACTGTTGCCTCAGTGTTGTTTGGTGCAATCTTCTGGCAAAAGGGGAAGGAAATAAATAATGAGCAGGATTTGCTGACTATACTTGGGTCCATGTACATTGCTGTAATATTCCTGGGAATAAACAATTGTTCAACAGTTCTTCCTTATGTGGCAACTGAGCGCACCGTTTTGTACCGCGAAAGATTTGCTGGGATGTACTCATCAAAGGCTTATTCCTTTGCACAGGTGGCCATGGAAATACCTTATACAATGTTGCAAGCAATTTTATATGTGGTCATTACATATCCTACGATAGGCTACTATTGGACTGCTTCCAAGGTTTTTTGGTACTTCTATGCAACCTTCTGTACATTTCTATACTTTGTATACCTTGGGATGTTGTTAGTTTCTTTGAGCTCAGACATGGAAGTAGCTTCGATATTGGCCACTGCAATCTACACCATATTGAATCTTTTCTCCGGCTTCCTCATGCCTGGACCGAAAATTCCGAAGTGGTGGATTTGGTGCTACTGGATCTGTCCTACATCATGGTCACTAAATGGCCTCCTGACTTCACAATATGGAGACATGGACAAAGAGATATTGATTTTTGGAGAGCATAAAACAGTTGCTTCCTTCCTACAAGATTATTATGGTTTTCATCATGATCTTTTACCCCTTGTGGCTATTGCTCTGATTACCTTCCCAATTGCTTTTGCTTTTCTCTTTGCCTATTTTATAGGGAGATTAAATTTCCAAAGGCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005911 GO:0006355 GO:0006464 GO:0006807 GO:0006808 GO:0007154 GO:0007584 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016597 GO:0018175 GO:0018177 GO:0019219 GO:0019222 GO:0019538 GO:0030054 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031406 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0036094 GO:0036211 GO:0042221 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071704 GO:0080090 GO:0090293 GO:1901564 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT ACT_6

Protein Analysis

1437

Amino Acids

161.81

Weight (kDa)

8.12

Isoelectric Point (pI)

37.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	174 - 355	1.6e-16	ABC transporter
ABC2_membrane	PF01061	510 - 721	4.3e-45	ABC-2 type transporter
PDR_assoc	PF08370	726 - 788	4.9e-23	Plant PDR ABC transporter associated
ABC_tran	PF00005	865 - 1017	2.2e-20	ABC transporter
ABC2_membrane	PF01061	1163 - 1376	3.4e-58	ABC-2 type transporter
ABC2_membrane_3	PF12698	1213 - 1421	8.8e-07	ABC-2 family transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0008235)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69040 AT1G69040
fragaria_vesca	FvH4_4g29340 FvH4_4g29340 FvH4_4g29340 FvH4_4g29340
malus_domestica	MD13G1056800.v1.1 MD16G1056200.v1.1
prunus_persica	Prupe.1G293100_v2.0.a1 Prupe.1G293100_v2.0.a1 Prupe.1G293100_v2.0.a1
pyrus_communis	pycom13g05070 pycom16g04920
rosa_chinensis	RchiOBHm_Chr4g0437401
rosa_laevigata	RLG00000006439
rosa_multiflora	Rmu_sc0006744.1_g000001
rosa_roxburghii	Rroxscaffold_5G00378650
rosa_rugosa	Rorug04G0301100 Rorug04G0301200
rosa_samantha	Rh4AG355400 Rh4BG365700 Rh4CG379900
rosa_wichuraiana	Rw4G031200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3372
AatII	GACGTC	1 cut(s)	30
AccB1I	GGYRCC	1 cut(s)	2854
AccB7I	CCANNNNNTGG	1 cut(s)	2464
AccBSI	CCGCTC	1 cut(s)	430
AccI	GTMKAC	1 cut(s)	3933
AccII	CGCG	1 cut(s)	3744
AciI	CCGC	7 cut(s)	428, 772, 1862, 2501, 3071, 3300, 3742
AclWI	GGATC	5 cut(s)	563, 845, 1347, 1995, 4088
AcoI	YGGCCR	4 cut(s)	997, 2953, 3795, 3990
AcsI	RAATTY	7 cut(s)	1397, 1573, 2159, 2217, 2885, 4050, 4297
AcuI	CTGAAG	7 cut(s)	656, 684, 1241, 1301, 1856, 2570, 3315
AcyI	GRCGYC	2 cut(s)	27, 537
AdeI	CACNNNGTG	1 cut(s)	1856
AfeI	AGCGCT	1 cut(s)	1050
AfiI	CCNNNNNNNGG	9 cut(s)	584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
AgeI	ACCGGT	1 cut(s)	2414
AjnI	CCWGG	6 cut(s)	1479, 2950, 3265, 3433, 3678, 4039
AjuI	GAANNNNNNNTTGG	2 cut(s)	708, 740
Alw21I	GWGCWC	4 cut(s)	1131, 1452, 2170, 3965
Alw26I	GTCTC	7 cut(s)	18, 501, 1172, 1708, 2355, 3406, 4125
Alw44I	GTGCAC	1 cut(s)	1127
AlwI	GGATC	5 cut(s)	563, 845, 1347, 1995, 4088
AlwNI	CAGNNNCTG	1 cut(s)	1175
Ama87I	CYCGRG	1 cut(s)	577
Aor51HI	AGCGCT	1 cut(s)	1050
AoxI	GGCC	9 cut(s)	997, 1477, 2618, 2953, 2962, 3223, 3795, 3990, 4108
ApaLI	GTGCAC	1 cut(s)	1127
ApeKI	GCWGC	9 cut(s)	109, 119, 313, 1304, 1555, 1603, 2144, 2590, 3068
ApoI	RAATTY	7 cut(s)	1397, 1573, 2159, 2217, 2885, 4050, 4297
AsiGI	ACCGGT	1 cut(s)	2414
Asp700I	GAANNNNTTC	3 cut(s)	2159, 2756, 4019
AspLEI	GCGC	4 cut(s)	1051, 1603, 2982, 3729
AsuC2I	CCSGG	4 cut(s)	578, 579, 1476, 2622
AsuHPI	GGTGA	5 cut(s)	188, 385, 980, 1464, 3421
AsuII	TTCGAA	3 cut(s)	475, 1266, 2366
AvaI	CYCGRG	1 cut(s)	577
AvaII	GGWCC	8 cut(s)	156, 572, 1037, 1238, 1809, 3437, 3654, 4043
BaeGI	GKGCMC	2 cut(s)	1131, 1289
BaeI	ACNNNNGTAYC	4 cut(s)	370, 403, 2033, 2066
BalI	TGGCCA	4 cut(s)	999, 2955, 3797, 3992
BanI	GGYRCC	1 cut(s)	2854
BanII	GRGCYC	2 cut(s)	1452, 3965
BarI	GAAGNNNNNNTAC	2 cut(s)	1214, 1246
BauI	CACGAG	1 cut(s)	2352
BbsI	GAAGAC	1 cut(s)	2759
Bbv12I	GWGCWC	4 cut(s)	1131, 1452, 2170, 3965
BbvCI	CCTCAGC	1 cut(s)	152
BbvI	GCAGC	9 cut(s)	96, 106, 300, 1291, 1542, 1615, 2131, 2577, 3080
BccI	CCATC	6 cut(s)	1648, 2169, 2476, 2635, 3028, 3234
BcgI	CGANNNNNNTGC	4 cut(s)	194, 228, 3734, 3768
BciT130I	CCWGG	6 cut(s)	1481, 2952, 3267, 3435, 3680, 4041
BclI	TGATCA	1 cut(s)	2866
BcnI	CCSGG	4 cut(s)	578, 579, 1476, 2622
BcoDI	GTCTC	7 cut(s)	18, 501, 1172, 1708, 2355, 3406, 4125
BfaI	CTAG	9 cut(s)	674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
BfmI	CTRYAG	4 cut(s)	110, 949, 1115, 1305
BfoI	RGCGCY	1 cut(s)	1052
BglI	GCCNNNNNGGC	1 cut(s)	2961
BlpI	GCTNAGC	1 cut(s)	105
Bme18I	GGWCC	8 cut(s)	156, 572, 1037, 1238, 1809, 3437, 3654, 4043
BmeT110I	CYCGRG	1 cut(s)	577
BmiI	GGNNCC	4 cut(s)	574, 1039, 2856, 3655
BmsI	GCATC	8 cut(s)	271, 493, 921, 961, 1849, 1963, 1970, 3049
BpiI	GAAGAC	1 cut(s)	2759
BplI	GAGNNNNNCTC	2 cut(s)	1313, 1345
BpmI	CTGGAG	3 cut(s)	2427, 2721, 3547
Bpu10I	CCTNAGC	1 cut(s)	152
Bpu1102I	GCTNAGC	1 cut(s)	105
Bpu14I	TTCGAA	3 cut(s)	475, 1266, 2366
BpuEI	CTTGAG	1 cut(s)	483
BpuMI	CCSGG	4 cut(s)	578, 579, 1476, 2622
BsaAI	YACGTR	2 cut(s)	730, 1646
BsaHI	GRCGYC	2 cut(s)	27, 537
BsaWI	WCCGGW	2 cut(s)	2414, 2548
BsaXI	ACNNNNNCTCC	4 cut(s)	362, 392, 2811, 2841
Bsc4I	CCNNNNNNNGG	9 cut(s)	584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
Bse118I	RCCGGY	1 cut(s)	2414
Bse3DI	GCAATG	1 cut(s)	3663
BseBI	CCWGG	6 cut(s)	1481, 2952, 3267, 3435, 3680, 4041
BseLI	CCNNNNNNNGG	9 cut(s)	584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
BseMI	GCAATG	1 cut(s)	3663
BseRI	GAGGAG	2 cut(s)	104, 2160
BseSI	GKGCMC	2 cut(s)	1131, 1289
BseXI	GCAGC	9 cut(s)	96, 106, 300, 1291, 1542, 1615, 2131, 2577, 3080
BseYI	CCCAGC	3 cut(s)	2657, 3480, 3755
BsgI	GTGCAG	4 cut(s)	234, 1602, 2546, 3506
Bsh1236I	CGCG	1 cut(s)	3744
BshFI	GGCC	9 cut(s)	999, 1479, 2620, 2955, 2964, 3225, 3797, 3992, 4110
BshNI	GGYRCC	1 cut(s)	2854
BshTI	ACCGGT	1 cut(s)	2414
BsiHKAI	GWGCWC	4 cut(s)	1131, 1452, 2170, 3965
BsiHKCI	CYCGRG	1 cut(s)	577
BsiSI	CCGG	7 cut(s)	578, 615, 1475, 2415, 2549, 2621, 4026
BslFI	GGGAC	2 cut(s)	558, 1023
BslI	CCNNNNNNNGG	9 cut(s)	584, 1298, 1486, 2005, 2464, 3050, 3306, 3434, 3860
BsmAI	GTCTC	7 cut(s)	18, 501, 1172, 1708, 2355, 3406, 4125
BsmBI	CGTCTC	1 cut(s)	18
BsmFI	GGGAC	2 cut(s)	558, 1023
BsmI	GAATGC	1 cut(s)	2946
BsnI	GGCC	9 cut(s)	999, 1479, 2620, 2955, 2964, 3225, 3797, 3992, 4110
BsoBI	CYCGRG	1 cut(s)	577
Bsp119I	TTCGAA	3 cut(s)	475, 1266, 2366
Bsp1286I	GDGCHC	5 cut(s)	1131, 1289, 1452, 2170, 3965
Bsp1407I	TGTACA	2 cut(s)	3660, 3914
Bsp1720I	GCTNAGC	1 cut(s)	105
Bsp19I	CCATGG	4 cut(s)	1234, 2004, 2463, 3798
BspACI	CCGC	7 cut(s)	428, 772, 1862, 2501, 3071, 3300, 3742
BspANI	GGCC	9 cut(s)	999, 1479, 2620, 2955, 2964, 3225, 3797, 3992, 4110
BspFNI	CGCG	1 cut(s)	3744
BspHI	TCATGA	2 cut(s)	3564, 4210
BspLI	GGNNCC	4 cut(s)	574, 1039, 2856, 3655
BspMAI	CTGCAG	3 cut(s)	114, 953, 1309
BspPI	GGATC	5 cut(s)	563, 845, 1347, 1995, 4088
BspT104I	TTCGAA	3 cut(s)	475, 1266, 2366
BspT107I	GGYRCC	1 cut(s)	2854
BsrBI	CCGCTC	1 cut(s)	430
BsrDI	GCAATG	1 cut(s)	3663
BsrFI	RCCGGY	1 cut(s)	2414
BsrGI	TGTACA	2 cut(s)	3660, 3914
BssAI	RCCGGY	1 cut(s)	2414
BssNAI	GTATAC	1 cut(s)	3934
BssNI	GRCGYC	2 cut(s)	27, 537
BssSI	CACGAG	1 cut(s)	2352
BssT1I	CCWWGG	8 cut(s)	1234, 1292, 2004, 2205, 2463, 3798, 3882, 3937
Bst1107I	GTATAC	1 cut(s)	3934
Bst2BI	CACGAG	1 cut(s)	2352
Bst2UI	CCWGG	6 cut(s)	1481, 2952, 3267, 3435, 3680, 4041
Bst6I	CTCTTC	3 cut(s)	201, 456, 3334
BstACI	GRCGYC	2 cut(s)	27, 537
BstAPI	GCANNNNNTGC	2 cut(s)	1126, 3637
BstAUI	TGTACA	2 cut(s)	3660, 3914
BstBAI	YACGTR	2 cut(s)	730, 1646
BstBI	TTCGAA	3 cut(s)	475, 1266, 2366
BstC8I	GCNNGC	6 cut(s)	772, 1540, 1931, 3087, 3499, 3825
BstDSI	CCRYGG	4 cut(s)	1234, 2004, 2463, 3798
BstENI	CCTNNNNNAGG	2 cut(s)	3048, 3858
BstFNI	CGCG	1 cut(s)	3744
BstH2I	RGCGCY	1 cut(s)	1052
BstHHI	GCGC	4 cut(s)	1051, 1603, 2982, 3729
BstMAI	GTCTC	7 cut(s)	18, 501, 1172, 1708, 2355, 3406, 4125
BstNI	CCWGG	6 cut(s)	1481, 2952, 3267, 3435, 3680, 4041
BstNSI	RCATGY	1 cut(s)	3501
BstSFI	CTRYAG	4 cut(s)	110, 949, 1115, 1305
BstSLI	GKGCMC	2 cut(s)	1131, 1289
BstSNI	TACGTA	1 cut(s)	1646
BstUI	CGCG	1 cut(s)	3744
BstV1I	GCAGC	9 cut(s)	96, 106, 300, 1291, 1542, 1615, 2131, 2577, 3080
BstV2I	GAAGAC	1 cut(s)	2759
BstX2I	RGATCY	1 cut(s)	4080
BstYI	RGATCY	1 cut(s)	4080
BstZ17I	GTATAC	1 cut(s)	3934
BsuRI	GGCC	9 cut(s)	999, 1479, 2620, 2955, 2964, 3225, 3797, 3992, 4110
BtgI	CCRYGG	4 cut(s)	1234, 2004, 2463, 3798
BtsI	GCAGTG	4 cut(s)	119, 958, 1614, 3993
Cac8I	GCNNGC	6 cut(s)	772, 1540, 1931, 3087, 3499, 3825
CaiI	CAGNNNCTG	1 cut(s)	1175
CciI	TCATGA	2 cut(s)	3564, 4210
CfoI	GCGC	4 cut(s)	1051, 1603, 2982, 3729
Cfr10I	RCCGGY	1 cut(s)	2414
Cfr9I	CCCGGG	1 cut(s)	577
CseI	GACGC	2 cut(s)	445, 526
CspAI	ACCGGT	1 cut(s)	2414
DraIII	CACNNNGTG	1 cut(s)	1856
EaeI	YGGCCR	4 cut(s)	997, 2953, 3795, 3990
Eam1104I	CTCTTC	3 cut(s)	201, 456, 3334
EarI	CTCTTC	3 cut(s)	201, 456, 3334
Ecl136II	GAGCTC	2 cut(s)	1450, 3963
Eco105I	TACGTA	1 cut(s)	1646
Eco130I	CCWWGG	8 cut(s)	1234, 1292, 2004, 2205, 2463, 3798, 3882, 3937
Eco24I	GRGCYC	2 cut(s)	1452, 3965
Eco32I	GATATC	3 cut(s)	946, 2265, 3154
Eco47I	GGWCC	8 cut(s)	156, 572, 1037, 1238, 1809, 3437, 3654, 4043
Eco47III	AGCGCT	1 cut(s)	1050
Eco53kI	GAGCTC	2 cut(s)	1450, 3963
Eco57I	CTGAAG	7 cut(s)	656, 684, 1241, 1301, 1856, 2570, 3315
Eco88I	CYCGRG	1 cut(s)	577
EcoICRI	GAGCTC	2 cut(s)	1450, 3963
EcoNI	CCTNNNNNAGG	2 cut(s)	3048, 3858
EcoO109I	RGGNCCY	1 cut(s)	156
EcoRI	GAATTC	1 cut(s)	2159
EcoRII	CCWGG	6 cut(s)	1479, 2950, 3265, 3433, 3678, 4039
EcoRV	GATATC	3 cut(s)	946, 2265, 3154
EcoT14I	CCWWGG	8 cut(s)	1234, 1292, 2004, 2205, 2463, 3798, 3882, 3937
EcoT22I	ATGCAT	1 cut(s)	976
EcoT38I	GRGCYC	2 cut(s)	1452, 3965
ErhI	CCWWGG	8 cut(s)	1234, 1292, 2004, 2205, 2463, 3798, 3882, 3937
Esp3I	CGTCTC	1 cut(s)	18
FalI	AAGNNNNNCTT	2 cut(s)	2920, 2952
FaqI	GGGAC	2 cut(s)	558, 1023
FauI	CCCGC	2 cut(s)	779, 3307
FauNDI	CATATG	1 cut(s)	1462
FbaI	TGATCA	1 cut(s)	2866
FblI	GTMKAC	1 cut(s)	3933
FriOI	GRGCYC	2 cut(s)	1452, 3965
FspBI	CTAG	9 cut(s)	674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
GlaI	GCGC	4 cut(s)	1050, 1602, 2981, 3728
GsaI	CCCAGC	3 cut(s)	2661, 3484, 3759
GsuI	CTGGAG	3 cut(s)	2427, 2721, 3547
HaeII	RGCGCY	1 cut(s)	1052
HaeIII	GGCC	9 cut(s)	999, 1479, 2620, 2955, 2964, 3225, 3797, 3992, 4110
HapII	CCGG	7 cut(s)	578, 615, 1475, 2415, 2549, 2621, 4026
HgaI	GACGC	2 cut(s)	445, 526
HhaI	GCGC	4 cut(s)	1051, 1603, 2982, 3729
Hin1I	GRCGYC	2 cut(s)	27, 537
Hin6I	GCGC	4 cut(s)	1049, 1601, 2980, 3727
HinP1I	GCGC	4 cut(s)	1049, 1601, 2980, 3727
HincII	GTYRAC	2 cut(s)	1440, 2539
HindII	GTYRAC	2 cut(s)	1440, 2539
HindIII	AAGCTT	3 cut(s)	51, 457, 1536
HpaI	GTTAAC	1 cut(s)	1440
HpaII	CCGG	7 cut(s)	578, 615, 1475, 2415, 2549, 2621, 4026
HphI	GGTGA	5 cut(s)	188, 385, 980, 1464, 3421
Hpy99I	CGWCG	1 cut(s)	439
HpyCH4IV	ACGT	4 cut(s)	27, 729, 1645, 3553
HpySE526I	ACGT	4 cut(s)	27, 729, 1645, 3553
Hsp92I	GRCGYC	2 cut(s)	27, 537
HspAI	GCGC	4 cut(s)	1049, 1601, 2980, 3727
Ksp22I	TGATCA	1 cut(s)	2866
KspAI	GTTAAC	1 cut(s)	1440
LmnI	GCTCC	6 cut(s)	4, 248, 435, 1938, 2173, 2609
Lsp1109I	GCAGC	9 cut(s)	96, 106, 300, 1291, 1542, 1615, 2131, 2577, 3080
LweI	GCATC	8 cut(s)	271, 493, 921, 961, 1849, 1963, 1970, 3049
MaeI	CTAG	9 cut(s)	674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
MaeII	ACGT	4 cut(s)	27, 729, 1645, 3553
MaeIII	GTNAC	9 cut(s)	235, 665, 953, 1105, 1155, 1421, 2774, 3319, 4098
MbiI	CCGCTC	1 cut(s)	430
MfeI	CAATTG	5 cut(s)	2910, 2994, 3075, 3691, 4254
MflI	RGATCY	1 cut(s)	4080
MhlI	GDGCHC	5 cut(s)	1131, 1289, 1452, 2170, 3965
MlsI	TGGCCA	4 cut(s)	999, 2955, 3797, 3992
MluNI	TGGCCA	4 cut(s)	999, 2955, 3797, 3992
MlyI	GAGTC	4 cut(s)	71, 1255, 2655, 2831
MmeI	TCCRAC	1 cut(s)	766
Mox20I	TGGCCA	4 cut(s)	999, 2955, 3797, 3992
Mph1103I	ATGCAT	1 cut(s)	976
MroXI	GAANNNNTTC	3 cut(s)	2159, 2756, 4019
MscI	TGGCCA	4 cut(s)	999, 2955, 3797, 3992
MslI	CAYNNNNRTG	4 cut(s)	869, 1972, 3502, 3563
Msp20I	TGGCCA	4 cut(s)	999, 2955, 3797, 3992
MspA1I	CMGCKG	6 cut(s)	109, 774, 2447, 2503, 2590, 3071
MspI	CCGG	7 cut(s)	578, 615, 1475, 2415, 2549, 2621, 4026
MunI	CAATTG	5 cut(s)	2910, 2994, 3075, 3691, 4254
Mva1269I	GAATGC	1 cut(s)	2946
MvaI	CCWGG	6 cut(s)	1481, 2952, 3267, 3435, 3680, 4041
MvnI	CGCG	1 cut(s)	3744
NciI	CCSGG	4 cut(s)	578, 579, 1476, 2622
NcoI	CCATGG	4 cut(s)	1234, 2004, 2463, 3798
NdeI	CATATG	1 cut(s)	1462
NlaIV	GGNNCC	4 cut(s)	574, 1039, 2856, 3655
NmeAIII	GCCGAG	1 cut(s)	427
NmuCI	GTSAC	4 cut(s)	953, 1105, 1155, 4098
NsiI	ATGCAT	1 cut(s)	976
NspI	RCATGY	1 cut(s)	3501
NspV	TTCGAA	3 cut(s)	475, 1266, 2366
PaeI	GCATGC	1 cut(s)	3501
PagI	TCATGA	2 cut(s)	3564, 4210
PcsI	WCGNNNNNNNCGW	2 cut(s)	180, 726
PctI	GAATGC	1 cut(s)	2946
PdmI	GAANNNNTTC	3 cut(s)	2159, 2756, 4019
PfeI	GAWTC	6 cut(s)	1840, 2109, 2399, 2933, 3377, 4015
PflMI	CCANNNNNTGG	1 cut(s)	2464
PinAI	ACCGGT	1 cut(s)	2414
PleI	GAGTC	4 cut(s)	71, 1254, 2654, 2830
PpsI	GAGTC	4 cut(s)	71, 1254, 2654, 2830
Ppu21I	YACGTR	2 cut(s)	730, 1646
PpuMI	RGGWCCY	1 cut(s)	156
PsiI	TTATAA	1 cut(s)	3372
Psp124BI	GAGCTC	2 cut(s)	1452, 3965
Psp5II	RGGWCCY	1 cut(s)	156
Psp6I	CCWGG	6 cut(s)	1479, 2950, 3265, 3433, 3678, 4039
PspFI	CCCAGC	3 cut(s)	2657, 3480, 3755
PspGI	CCWGG	6 cut(s)	1479, 2950, 3265, 3433, 3678, 4039
PspN4I	GGNNCC	4 cut(s)	574, 1039, 2856, 3655
PspPPI	RGGWCCY	1 cut(s)	156
PstI	CTGCAG	3 cut(s)	114, 953, 1309
PstNI	CAGNNNCTG	1 cut(s)	1175
PsuI	RGATCY	1 cut(s)	4080
PvuII	CAGCTG	3 cut(s)	109, 2447, 2590
RseI	CAYNNNNRTG	4 cut(s)	869, 1972, 3502, 3563
SacI	GAGCTC	2 cut(s)	1452, 3965
SchI	GAGTC	4 cut(s)	71, 1255, 2655, 2831
SduI	GDGCHC	5 cut(s)	1131, 1289, 1452, 2170, 3965
SfaNI	GCATC	8 cut(s)	271, 493, 921, 961, 1849, 1963, 1970, 3049
SfcI	CTRYAG	4 cut(s)	110, 949, 1115, 1305
SfiI	GGCCNNNNNGGCC	1 cut(s)	2961
SfuI	TTCGAA	3 cut(s)	475, 1266, 2366
SinI	GGWCC	8 cut(s)	156, 572, 1037, 1238, 1809, 3437, 3654, 4043
SmaI	CCCGGG	1 cut(s)	579
SmiMI	CAYNNNNRTG	4 cut(s)	869, 1972, 3502, 3563
SmlI	CTYRAG	1 cut(s)	498
SmoI	CTYRAG	1 cut(s)	498
SnaBI	TACGTA	1 cut(s)	1646
SphI	GCATGC	1 cut(s)	3501
SsiI	CCGC	7 cut(s)	428, 772, 1862, 2501, 3071, 3300, 3742
SspI	AATATT	3 cut(s)	3030, 3251, 3675
SspMI	CTAG	9 cut(s)	674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
SstI	GAGCTC	2 cut(s)	1452, 3965
StyI	CCWWGG	8 cut(s)	1234, 1292, 2004, 2205, 2463, 3798, 3882, 3937
TaiI	ACGT	4 cut(s)	30, 732, 1648, 3556
TaqII	GACCGA	2 cut(s)	1025, 4060
TatI	WGTACW	5 cut(s)	415, 2529, 3660, 3762, 3914
TauI	GCSGC	3 cut(s)	430, 2503, 3074
TfiI	GAWTC	6 cut(s)	1840, 2109, 2399, 2933, 3377, 4015
TseFI	GTSAC	4 cut(s)	953, 1105, 1155, 4098
TseI	GCWGC	9 cut(s)	109, 119, 313, 1304, 1555, 1603, 2144, 2590, 3068
Tsp45I	GTSAC	4 cut(s)	953, 1105, 1155, 4098
TspGWI	ACGGA	1 cut(s)	2153
TspMI	CCCGGG	1 cut(s)	577
Van91I	CCANNNNNTGG	1 cut(s)	2464
VneI	GTGCAC	1 cut(s)	1127
VpaK11BI	GGWCC	8 cut(s)	156, 572, 1037, 1238, 1809, 3437, 3654, 4043
XagI	CCTNNNNNAGG	2 cut(s)	3048, 3858
XapI	RAATTY	7 cut(s)	1397, 1573, 2159, 2217, 2885, 4050, 4297
XceI	RCATGY	1 cut(s)	3501
XcmI	CCANNNNNNNNNTGG	2 cut(s)	601, 3477
XmaI	CCCGGG	1 cut(s)	577
XmiI	GTMKAC	1 cut(s)	3933
XmnI	GAANNNNTTC	3 cut(s)	2159, 2756, 4019
XspI	CTAG	9 cut(s)	674, 809, 935, 941, 1758, 2763, 3056, 3144, 3548
ZraI	GACGTC	1 cut(s)	28
Zsp2I	ATGCAT	1 cut(s)	976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.