Rorug04G0409400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004788
Physical Location & Seq
Reverse (-)
57177513 .. 57185216
7704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0409400.1

Sequence Viewer

Length: 4014 bp
ATGGCGGCGTCGCAGACCATCGACGTCCGGAATCTCAATCGCCACAACCGCGAGCTCGTCCTCAAGAAGGCCCTCGCCACTGACGATCAGGACAACCAGCACCTCCTCGCCGGAATAAAAGAACGCCTCGACAGAGTGGGATTGGAGGTTCCGGCAGTAGAAGTACGCTTCCAGAATCTGAATATCGTGGCAGACGTTCAAACAGGATCAAGAGCTTTGCCTACTCTCATCAACTATATTCGTGATGGTATAGAGGGTATTTTAACTAGTTTCAGGATTGCTAAACCCAAGAGACGGTCTTTAACGATTTTGAACAACATCAGCGGCCTTATCAAACCCGGAAGGATGACTTTGCTTTTAGGACCCCCAAGTTCTGGCAAATCCACTTTGCTCATGACTCTTGCAGGAAAACTAGACTGCAACTTAAAGAATAGTGGTACCATCACATATAATGGCCATAAGCTTGATGAGTTCTGCGTTCAAAGAACATCTGCTTACATAAGCCAAACAGATAATCACATTGCAGAACTCACCGTAAGAGAAACCTTGGACTTTGCAGCTAGAGTTCAAGGTACAAGCGAAGGTTTTGCAGCGTGCTTGAAAGACCTTGTAAAACTGGAGCAGGAAAGACACATAACTCCAGATCCAAATATTGATGCCTTCATGAAGGCATCATCTGTTGGTAAAATTTCAACAGATTATGTTCTTAAAGTGCTCGGTCTTGATGTCTGTTCAGATACAATTGTCGGAAACAACATGATAAGAGGGGTTTCTGGGGGACAGAGGAAAAGGGTTACTACAGGTGAAATGACAGTTGGACCAAGAAAAGCCCTGTTTATGGATGAAATATCTACTGGACTGGATAGTTCTACTACATACCAAATTGTAAAATGTATTGGGAATTTTGTTCATCAGATGGAAGCTACAGTACTAATGGCTCTGCTTCAACCTGCACCTGAGACATTTGAATTGTTTGATGATCTAGTGCTCCTATCTGAAGGTCACGTGGTTTATCAAGGCCCTCGAGCAGAAGTGTTGGAGTTCTTCAAGTCATTAGGATTCAAACTACCCCCACGAAAGGGTATTGCGGATTTTCTTCAAGAGGTAACATCTAAAAAGGATCAAGCTCAGTACTGGGCTGATTGTTCGAAACCCTACACATACCTTTCTGTTTCAGAAATTGCAGAAGCCTTTAAGGAGTCCAGGTTTGGAAGATCTGTGGATGACACTCTCTCTGCTCCATATCATAAATCTAGCAGTCTTCCTTCGGCTTTGCCAAAAACTAAATATGCAACCTCAAAATGGGAGCTTTGTAAAGCATGCTTTTCGCGAGAACTACTGTTGATCAGCAGGCATAGGTTTCTTTACACATTTAGGACCTGCCAGGTTGCATTTGTTGGATTTGTGACATGCACAACGTTTCCTAGATGGCAATTACACCCCACAGATGAAATAAATGGCAACCTTTATCTTTCTTGCTTGTTCTTTGGGTTAGTGCACATGATGTTCAATGGGCTTTCCGAGCTACCTCTTATGATATCTCAGCTCCCAGTCTTTTACAAGCAAAGGGATAATTACTTTCATCCTGCGTGGGCATGGTCTGGTGTAAATTTGCTTCTGCGCATACCTTACTCTATTATTGAAGCTGTTGTATGGTCTTGTGTCGTATACTACACAGTTGGTTTTGCCCCTGGTGCTGGGAAATTTTTCCGTTTCATGTTTTTGCTATTTTCTGTGCACCAAATGGCTTTGGGTCTCTTCCGGACAGTGGCATCTCTTGCAAGAGATATGGTCATTGCCAATACATTTGGATCAGCTGCATTACTTGTTATATTTTTGTTGGGTGGATTCGTTATTCCCAAAGATAGCATTAAGCCATGGTGGCAATGGGCCTTTTGGGTATCACCACTATCATATGGACAACGCGCTCTTTCTGTCAACGAATTTACTGCTGTAAGGTGGATCAAGATAGCTCCTTTAACAAACAGAACAATTGGGCAAAATGTCCTCCAAGAACACAACTTACCAAATGGTGATTATTGGTATTGGATTGGAGTTGGTGCTCTGTTAGCTTATGCATTGCTTTTCAATTGCACAGTGACTCTGGCCTTGATGTACCTAAATCCACTAAGGAAGTACCAGACAGTGCTTCCACTTGATGACACAGAAGGGAGTCCTGCTGCAGATGGGGGTAAAAATAAGACTGTGTCTAGCCCAAAATCAGCTGGAGGTAACAGCCCAGTAAAGGGAATGATCTTACCATTTCAGCCATTAACAATGACTTTCCACAATGTTACCTACTCTGTTCACAAGCCAAAGGAGATGAAGTCACAAGCTTTAGTTGGGTCTAGTGGAGCTGGAAAGACCACTTTGATGGACGTCCTTGCTGGTAGGAAAACTGGTGGATACATAGAAGGAGATATTAAGATATCTGGTTACCCGAGAGAGCAACGTACTTTTGCTAGAGTAGCAGGATATGTTGAGCAAAATGACATACATTCTCCTCAAGTTACAGTTGAGGAGAGTCTATGGTTTTCTTCTGCTCTTCGCCTTCCAAAGGAAGTCAGCACAGAGACAAGACTCGTGTTTGTCGAAGAAGTAATGGAACTAGTAGAGCTTGATACTCTAAGGCATGCTTTGGTTGGTGTGCCAGGCAGTTCAGGCTTATCAACAGAGCAAAGAAAACGCTTAACAATCGCTGTTGAGCTTGTCGCCAACCCTTCAATTATCTTTATGGATGAACCCACATCTGGACTGGACGCAAGGGCAGCAGCCATTGTGATGAGGACTGTTCGTAATACTGTTGATACAGGAAGAACGGTGGTCTGCACTATACACCAACCAAGTATCGACATTTTTGAGGCATTTGACGAGCTTCTTCTTATGAAACGAGGGGGGCAAGTTATATATGGAGGAAAGCTAGGCCAGCACTCCCAGTCAATGATAAACTACTTTCAGGAGATTAATGGAATCACTCCGATTCCAAGCGGGTACAATCCCGCAACCTGGATGCTGGAGGTAACCACACCGGCTTGTGAACAGAGACTCGGAGAAGACTTCACTACCATATACAGGAATTCAGAGCAGTACAGGGAAGTGGAAGAGTCCATCAAGCAGTTGAGTACTCCTCCAACCGACTCAAAACCATTAAAGTTTGCTTCCACATATTCACAGAACACATTGGCTCAATTTAGGAACTGCTTATGGAAACAGAATCTCGTGTATTGGAGAAGTCCACAATATAATGCGATGAGGTTAATCTTTACTACCCTTGCTGCATTGATACTGGGTTCGGCATTTTGGGATGTCGGTACAGAAAGGGACTCGCTCCAATCACTGATGATGGTTATGGGAGCTCTATATTCTGCTGGTTTGTTTCTTGGGGTAAATAACGCTTCTTCAGTTCAGCCGATTGTTTCGACTGAGAGGACGGTGTTTTATCGAGAGAAAGCAGCTGGAATGTATTCTCCTCTGGCTTATGCAGCGGCCCAGGGCCTTATAGAGATCCCATACATTGCTGTGCAGACAATAGTATACGGGGTGATCACATACTTCATGATCAACTTTGAAAGGACAATCACAAAATTCTTGCTCTATATTCTGATCATGTTCCTCACTTTCGTATATTTCACTCTTTTCGGCATGGCGGCTGTTGCCGTCACAGCCTCTCAACCCCTGGCAGCTGCCATCTCTGCTGCATTTTACTCATTGTGGAATCTATTCTCTGGTTTCCTCGTCCCCAAACCAAACATCCCGGTATGGTGGCTTTGGTTTTACTACATCTCCCCAGTTGCATGGACACTCAGAGGTATTATCACTTCCCAACTTGGTGATGTGGAGACCATTCTTGTCGGACCTGCGTTTAAGGGCACCTTGAAAGAATACTTGGAAGTCAGCCTTGGCTATGGCCCTGGGATGATTGGAGTTTCAGTAGCTGTGCTTCTGGGCTTCAACGTCCTTTTGTTCAGTATCTTTGCCTTCTCCATCAAATTTCTCAACTTCCAGAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1337

Amino Acids

149.03

Weight (kDa)

8.77

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 104 - 282 7.4e-17 ABC transporter
ABC2_membrane_7 PF19055 316 - 366 5.5e-06 ABC-2 type transporter
ABC2_membrane PF01061 437 - 649 2.9e-49 ABC-2 type transporter
PDR_assoc PF08370 654 - 716 5.5e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 777 - 917 8.6e-19 ABC transporter
ABC2_membrane PF01061 1063 - 1275 6.7e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 27, 2382
Acc16I TGCGCA 1 cut(s) 1622
Acc36I ACCTGC 3 cut(s) 958, 1388, 3865
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 2 cut(s) 437, 3869
AccB7I CCANNNNNTGG 1 cut(s) 374
AccI GTMKAC 2 cut(s) 1668, 3534
AccII CGCG 3 cut(s) 51, 1330, 1926
AccIII TCCGGA 2 cut(s) 27, 1761
AciI CCGC 8 cut(s) 5, 49, 324, 1088, 2988, 3000, 3485, 3647
AclI AACGTT 1 cut(s) 1418
AclWI GGATC 6 cut(s) 214, 638, 1128, 1819, 1970, 3499
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 8 cut(s) 687, 901, 1609, 1703, 1943, 3076, 3584, 3989
AcuI CTGAAG 2 cut(s) 1017, 3384
AcvI CACGTG 1 cut(s) 1006
AcyI GRCGYC 3 cut(s) 8, 24, 2379
AhlI ACTAGT 2 cut(s) 266, 2608
AjnI CCWGG 8 cut(s) 1202, 1383, 1690, 2650, 3005, 3489, 3675, 3910
AjuI GAANNNNNNNTTGG 6 cut(s) 798, 830, 3869, 3882, 3901, 3914
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
Alw21I GWGCWC 7 cut(s) 57, 717, 990, 1500, 1740, 2065, 3358
Alw26I GTCTC 6 cut(s) 286, 953, 1760, 2567, 3037, 3833
Alw44I GTGCAC 2 cut(s) 1496, 1736
AlwI GGATC 6 cut(s) 214, 638, 1128, 1819, 1970, 3499
AlwNI CAGNNNCTG 2 cut(s) 178, 3935
Ama87I CYCGRG 2 cut(s) 1023, 2440
Aor13HI TCCGGA 2 cut(s) 27, 1761
ApaLI GTGCAC 2 cut(s) 1496, 1736
ApoI RAATTY 8 cut(s) 687, 901, 1609, 1703, 1943, 3076, 3584, 3989
AseI ATTAAT 1 cut(s) 2964
Asp700I GAANNNNTTC 3 cut(s) 1706, 3463, 3719
Asp718I GGTACC 1 cut(s) 437
AspLEI GCGC 2 cut(s) 1623, 1928
AsuC2I CCSGG 2 cut(s) 339, 3755
AsuHPI GGTGA 6 cut(s) 523, 815, 1896, 2045, 3553, 3842
AsuII TTCGAA 1 cut(s) 1148
AvaI CYCGRG 2 cut(s) 1023, 2440
AvaII GGWCC 4 cut(s) 362, 818, 1377, 3854
BaeGI GKGCMC 3 cut(s) 1500, 1740, 3872
BaeI ACNNNNGTAYC 2 cut(s) 729, 762
BalI TGGCCA 1 cut(s) 456
BanI GGYRCC 2 cut(s) 437, 3869
BanII GRGCYC 2 cut(s) 57, 3358
BarI GAAGNNNNNNTAC 8 cut(s) 912, 944, 1599, 1631, 3802, 3834, 3924, 3956
BauI CACGAG 2 cut(s) 2582, 3218
BbrPI CACGTG 1 cut(s) 1006
BbsI GAAGAC 2 cut(s) 1253, 3060
Bbv12I GWGCWC 7 cut(s) 57, 717, 990, 1500, 1740, 2065, 3358
BceAI ACGGC 1 cut(s) 3641
BcgI CGANNNNNNTGC 6 cut(s) 569, 603, 1308, 1342, 1931, 1965
BciT130I CCWGG 8 cut(s) 1204, 1385, 1692, 2652, 3007, 3491, 3677, 3912
BciVI GTATCC 1 cut(s) 2399
BclI TGATCA 4 cut(s) 1344, 3543, 3558, 3603
BcnI CCSGG 2 cut(s) 339, 3755
BcoDI GTCTC 6 cut(s) 286, 953, 1760, 2567, 3037, 3833
BcuI ACTAGT 2 cut(s) 266, 2608
BfmI CTRYAG 3 cut(s) 798, 924, 2181
BfuAI ACCTGC 3 cut(s) 958, 1388, 3865
BfuI GTATCC 1 cut(s) 2399
BglI GCCNNNNNGGC 1 cut(s) 1882
BglII AGATCT 1 cut(s) 1214
BmcAI AGTACT 3 cut(s) 930, 1133, 3124
Bme18I GGWCC 4 cut(s) 362, 818, 1377, 3854
BmeT110I CYCGRG 2 cut(s) 1023, 2440
BmiI GGNNCC 4 cut(s) 150, 364, 439, 3871
BmrI ACTGGG 6 cut(s) 1144, 1544, 2234, 2929, 3296, 3782
BmsI GCATC 4 cut(s) 646, 680, 1781, 3000
BmuI ACTGGG 6 cut(s) 1144, 1544, 2234, 2929, 3296, 3782
BpiI GAAGAC 2 cut(s) 1253, 3060
BplI GAGNNNNNCTC 2 cut(s) 3112, 3144
BpmI CTGGAG 4 cut(s) 624, 638, 2247, 3035
Bpu14I TTCGAA 1 cut(s) 1148
BpuEI CTTGAG 2 cut(s) 47, 2490
BpuMI CCSGG 2 cut(s) 339, 3755
BsaAI YACGTR 1 cut(s) 1006
BsaHI GRCGYC 3 cut(s) 8, 24, 2379
BsaI GGTCTC 2 cut(s) 1760, 3833
BsaJI CCNNGG 9 cut(s) 546, 1690, 1877, 3489, 3490, 3675, 3898, 3910, 3911
BsaWI WCCGGW 2 cut(s) 27, 1761
BsaXI ACNNNNNCTCC 8 cut(s) 1190, 1220, 2312, 2342, 3767, 3797, 3915, 3945
Bse118I RCCGGY 1 cut(s) 3028
Bse3DI GCAATG 5 cut(s) 519, 1794, 1892, 2078, 3513
BseAI TCCGGA 2 cut(s) 27, 1761
BseBI CCWGG 8 cut(s) 1204, 1385, 1692, 2652, 3007, 3491, 3677, 3912
BseDI CCNNGG 9 cut(s) 546, 1690, 1877, 3489, 3490, 3675, 3898, 3910, 3911
BseGI GGATG 9 cut(s) 351, 847, 1228, 1582, 2743, 3015, 3310, 3750, 3921
BseMI GCAATG 5 cut(s) 519, 1794, 1892, 2078, 3513
BseMII CTCAG 5 cut(s) 948, 1142, 1556, 3414, 3817
BseRI GAGGAG 5 cut(s) 95, 2493, 2534, 3117, 3459
BseSI GKGCMC 3 cut(s) 1500, 1740, 3872
BseYI CCCAGC 1 cut(s) 1697
BsgI GTGCAG 3 cut(s) 936, 2812, 3542
Bsh1236I CGCG 3 cut(s) 51, 1330, 1926
BshNI GGYRCC 2 cut(s) 437, 3869
BsiHKAI GWGCWC 7 cut(s) 57, 717, 990, 1500, 1740, 2065, 3358
BsiHKCI CYCGRG 2 cut(s) 1023, 2440
BsiSI CCGG 7 cut(s) 28, 111, 152, 339, 1762, 3029, 3755
BslFI GGGAC 3 cut(s) 792, 3335, 3722
BsmAI GTCTC 6 cut(s) 286, 953, 1760, 2567, 3037, 3833
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 3 cut(s) 792, 3335, 3722
Bso31I GGTCTC 2 cut(s) 1760, 3833
BsoBI CYCGRG 2 cut(s) 1023, 2440
Bsp119I TTCGAA 1 cut(s) 1148
Bsp1286I GDGCHC 8 cut(s) 57, 717, 990, 1500, 1740, 2065, 3358, 3872
Bsp13I TCCGGA 2 cut(s) 27, 1761
Bsp19I CCATGG 1 cut(s) 1877
Bsp68I TCGCGA 1 cut(s) 1330
BspACI CCGC 8 cut(s) 5, 49, 324, 1088, 2988, 3000, 3485, 3647
BspCNI CTCAG 5 cut(s) 949, 1141, 1555, 3415, 3816
BspEI TCCGGA 2 cut(s) 27, 1761
BspFNI CGCG 3 cut(s) 51, 1330, 1926
BspHI TCATGA 3 cut(s) 393, 663, 3555
BspLI GGNNCC 4 cut(s) 150, 364, 439, 3871
BspMAI CTGCAG 1 cut(s) 2185
BspMI ACCTGC 3 cut(s) 958, 1388, 3865
BspPI GGATC 6 cut(s) 214, 638, 1128, 1819, 1970, 3499
BspQI GCTCTTC 1 cut(s) 2550
BspT104I TTCGAA 1 cut(s) 1148
BspT107I GGYRCC 2 cut(s) 437, 3869
BspTNI GGTCTC 2 cut(s) 1760, 3833
BsrDI GCAATG 5 cut(s) 519, 1794, 1892, 2078, 3513
BsrFI RCCGGY 1 cut(s) 3028
BssAI RCCGGY 1 cut(s) 3028
BssECI CCNNGG 9 cut(s) 546, 1690, 1877, 3489, 3490, 3675, 3898, 3910, 3911
BssNAI GTATAC 2 cut(s) 1669, 3535
BssNI GRCGYC 3 cut(s) 8, 24, 2379
BssSI CACGAG 2 cut(s) 2582, 3218
BssT1I CCWWGG 3 cut(s) 546, 1877, 3898
Bst1107I GTATAC 2 cut(s) 1669, 3535
Bst2BI CACGAG 2 cut(s) 2582, 3218
Bst2UI CCWGG 8 cut(s) 1204, 1385, 1692, 2652, 3007, 3491, 3677, 3912
Bst6I CTCTTC 3 cut(s) 1763, 2550, 3096
BstACI GRCGYC 3 cut(s) 8, 24, 2379
BstAPI GCANNNNNTGC 1 cut(s) 1778
BstBAI YACGTR 1 cut(s) 1006
BstBI TTCGAA 1 cut(s) 1148
BstC8I GCNNGC 6 cut(s) 53, 595, 1321, 1352, 2634, 2927
BstDEI CTNAG 7 cut(s) 957, 1128, 1542, 2129, 2627, 3423, 3803
BstDSI CCRYGG 1 cut(s) 1877
BstEII GGTNACC 2 cut(s) 2435, 3019
BstENI CCTNNNNNAGG 2 cut(s) 65, 2555
BstF5I GGATG 9 cut(s) 351, 847, 1228, 1582, 2743, 3015, 3310, 3750, 3921
BstFNI CGCG 3 cut(s) 51, 1330, 1926
BstHHI GCGC 2 cut(s) 1623, 1928
BstMAI GTCTC 6 cut(s) 286, 953, 1760, 2567, 3037, 3833
BstNI CCWGG 8 cut(s) 1204, 1385, 1692, 2652, 3007, 3491, 3677, 3912
BstNSI RCATGY 3 cut(s) 1323, 1413, 2636
BstPI GGTNACC 2 cut(s) 2435, 3019
BstSFI CTRYAG 3 cut(s) 798, 924, 2181
BstSLI GKGCMC 3 cut(s) 1500, 1740, 3872
BstUI CGCG 3 cut(s) 51, 1330, 1926
BstV2I GAAGAC 2 cut(s) 1253, 3060
BstX2I RGATCY 3 cut(s) 643, 1214, 3504
BstXI CCANNNNNNTGG 2 cut(s) 2374, 3795
BstYI RGATCY 3 cut(s) 643, 1214, 3504
BstZ17I GTATAC 2 cut(s) 1669, 3535
BsuI GTATCC 1 cut(s) 2399
BtgI CCRYGG 1 cut(s) 1877
BtgZI GCGATG 1 cut(s) 3263
BtsCI GGATG 9 cut(s) 351, 847, 1228, 1582, 2743, 3015, 3310, 3750, 3921
BtsIMutI CAGTG 5 cut(s) 78, 1773, 2103, 2151, 3335
BtuMI TCGCGA 1 cut(s) 1330
BveI ACCTGC 3 cut(s) 958, 1388, 3865
Cac8I GCNNGC 6 cut(s) 53, 595, 1321, 1352, 2634, 2927
CaiI CAGNNNCTG 2 cut(s) 178, 3935
CciI TCATGA 3 cut(s) 393, 663, 3555
CfoI GCGC 2 cut(s) 1623, 1928
Cfr10I RCCGGY 1 cut(s) 3028
CseI GACGC 1 cut(s) 2768
DdeI CTNAG 7 cut(s) 957, 1128, 1542, 2129, 2627, 3423, 3803
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 3 cut(s) 1763, 2550, 3096
EarI CTCTTC 3 cut(s) 1763, 2550, 3096
Ecl136II GAGCTC 2 cut(s) 55, 3356
Eco130I CCWWGG 3 cut(s) 546, 1877, 3898
Eco24I GRGCYC 2 cut(s) 57, 3358
Eco31I GGTCTC 2 cut(s) 1760, 3833
Eco32I GATATC 2 cut(s) 1539, 2430
Eco47I GGWCC 4 cut(s) 362, 818, 1377, 3854
Eco53kI GAGCTC 2 cut(s) 55, 3356
Eco57I CTGAAG 2 cut(s) 1017, 3384
Eco72I CACGTG 1 cut(s) 1006
Eco88I CYCGRG 2 cut(s) 1023, 2440
Eco91I GGTNACC 2 cut(s) 2435, 3019
EcoICRI GAGCTC 2 cut(s) 55, 3356
EcoNI CCTNNNNNAGG 2 cut(s) 65, 2555
EcoO109I RGGNCCY 5 cut(s) 70, 362, 1019, 1377, 3493
EcoO65I GGTNACC 2 cut(s) 2435, 3019
EcoRI GAATTC 1 cut(s) 3076
EcoRII CCWGG 8 cut(s) 1202, 1383, 1690, 2650, 3005, 3489, 3675, 3910
EcoRV GATATC 2 cut(s) 1539, 2430
EcoT14I CCWWGG 3 cut(s) 546, 1877, 3898
EcoT22I ATGCAT 1 cut(s) 2080
EcoT38I GRGCYC 2 cut(s) 57, 3358
ErhI CCWWGG 3 cut(s) 546, 1877, 3898
Esp3I CGTCTC 1 cut(s) 286
FalI AAGNNNNNCTT 8 cut(s) 334, 366, 2620, 2652, 3857, 3889, 3882, 3914
FaqI GGGAC 3 cut(s) 792, 3335, 3722
FauI CCCGC 2 cut(s) 2981, 3007
FauNDI CATATG 1 cut(s) 1915
FbaI TGATCA 4 cut(s) 1344, 3543, 3558, 3603
FblI GTMKAC 2 cut(s) 1668, 3534
FokI GGATG 9 cut(s) 358, 854, 1235, 1569, 2750, 3022, 3317, 3737, 3928
FriOI GRGCYC 2 cut(s) 57, 3358
FspI TGCGCA 1 cut(s) 1622
GlaI GCGC 2 cut(s) 1622, 1927
GsaI CCCAGC 1 cut(s) 1701
GsuI CTGGAG 4 cut(s) 624, 638, 2247, 3035
HapII CCGG 7 cut(s) 28, 111, 152, 339, 1762, 3029, 3755
HgaI GACGC 1 cut(s) 2768
HhaI GCGC 2 cut(s) 1623, 1928
Hin1I GRCGYC 3 cut(s) 8, 24, 2379
Hin6I GCGC 2 cut(s) 1621, 1926
HinP1I GCGC 2 cut(s) 1621, 1926
HincII GTYRAC 1 cut(s) 1939
HindII GTYRAC 1 cut(s) 1939
HindIII AAGCTT 2 cut(s) 461, 2334
HpaII CCGG 7 cut(s) 28, 111, 152, 339, 1762, 3029, 3755
HphI GGTGA 6 cut(s) 523, 815, 1896, 2045, 3553, 3842
Hpy166II GTNNAC 8 cut(s) 1498, 1669, 1738, 1939, 2308, 3038, 3236, 3535
Hpy8I GTNNAC 8 cut(s) 1498, 1669, 1738, 1939, 2308, 3038, 3236, 3535
Hpy99I CGWCG 2 cut(s) 13, 26
HpyCH4IV ACGT 7 cut(s) 24, 195, 1005, 1418, 2379, 2452, 3954
HpyF3I CTNAG 7 cut(s) 957, 1128, 1542, 2129, 2627, 3423, 3803
HpySE526I ACGT 7 cut(s) 24, 195, 1005, 1418, 2379, 2452, 3954
Hsp92I GRCGYC 3 cut(s) 8, 24, 2379
HspAI GCGC 2 cut(s) 1621, 1926
Kpn2I TCCGGA 2 cut(s) 27, 1761
KpnI GGTACC 1 cut(s) 441
Ksp22I TGATCA 4 cut(s) 1344, 3543, 3558, 3603
LguI GCTCTTC 1 cut(s) 2550
LmnI GCTCC 9 cut(s) 619, 993, 1243, 1306, 1551, 1978, 2354, 3333, 3353
LweI GCATC 4 cut(s) 646, 680, 1781, 3000
MaeII ACGT 7 cut(s) 24, 195, 1005, 1418, 2379, 2452, 3954
MfeI CAATTG 3 cut(s) 741, 1992, 2089
MflI RGATCY 3 cut(s) 643, 1214, 3504
MhlI GDGCHC 8 cut(s) 57, 717, 990, 1500, 1740, 2065, 3358, 3872
MlsI TGGCCA 1 cut(s) 456
MluNI TGGCCA 1 cut(s) 456
MmeI TCCRAC 6 cut(s) 727, 796, 1017, 1378, 3155, 3832
Mox20I TGGCCA 1 cut(s) 456
Mph1103I ATGCAT 1 cut(s) 2080
MroI TCCGGA 2 cut(s) 27, 1761
MroXI GAANNNNTTC 3 cut(s) 1706, 3463, 3719
MscI TGGCCA 1 cut(s) 456
MslI CAYNNNNRTG 3 cut(s) 2372, 2780, 3518
Msp20I TGGCCA 1 cut(s) 456
MspA1I CMGCKG 6 cut(s) 324, 1817, 2225, 3455, 3485, 3683
MspI CCGG 7 cut(s) 28, 111, 152, 339, 1762, 3029, 3755
MunI CAATTG 3 cut(s) 741, 1992, 2089
MvaI CCWGG 8 cut(s) 1204, 1385, 1692, 2652, 3007, 3491, 3677, 3912
MvnI CGCG 3 cut(s) 51, 1330, 1926
NciI CCSGG 2 cut(s) 339, 3755
NcoI CCATGG 1 cut(s) 1877
NdeI CATATG 1 cut(s) 1915
NlaIV GGNNCC 4 cut(s) 150, 364, 439, 3871
NmuCI GTSAC 5 cut(s) 1001, 1405, 2098, 2328, 3658
NruI TCGCGA 1 cut(s) 1330
NsbI TGCGCA 1 cut(s) 1622
NsiI ATGCAT 1 cut(s) 2080
NspI RCATGY 3 cut(s) 1323, 1413, 2636
NspV TTCGAA 1 cut(s) 1148
PaeI GCATGC 2 cut(s) 1323, 2636
PaeR7I CTCGAG 1 cut(s) 1023
PagI TCATGA 3 cut(s) 393, 663, 3555
PasI CCCWGGG 2 cut(s) 3490, 3911
PciSI GCTCTTC 1 cut(s) 2550
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 192, 2589
PdmI GAANNNNTTC 3 cut(s) 1706, 3463, 3719
PfeI GAWTC 8 cut(s) 31, 175, 1059, 1848, 2970, 2980, 3214, 3715
PflFI GACNNNGTC 1 cut(s) 2206
PflMI CCANNNNNTGG 1 cut(s) 374
PmaCI CACGTG 1 cut(s) 1006
PmlI CACGTG 1 cut(s) 1006
Ppu21I YACGTR 1 cut(s) 1006
PpuMI RGGWCCY 2 cut(s) 362, 1377
PshBI ATTAAT 1 cut(s) 2964
Psp124BI GAGCTC 2 cut(s) 57, 3358
Psp1406I AACGTT 1 cut(s) 1418
Psp5II RGGWCCY 2 cut(s) 362, 1377
Psp6I CCWGG 8 cut(s) 1202, 1383, 1690, 2650, 3005, 3489, 3675, 3910
PspCI CACGTG 1 cut(s) 1006
PspEI GGTNACC 2 cut(s) 2435, 3019
PspFI CCCAGC 1 cut(s) 1697
PspGI CCWGG 8 cut(s) 1202, 1383, 1690, 2650, 3005, 3489, 3675, 3910
PspN4I GGNNCC 4 cut(s) 150, 364, 439, 3871
PspPPI RGGWCCY 2 cut(s) 362, 1377
PspXI VCTCGAGB 1 cut(s) 1023
PsrI GAACNNNNNNTAC 2 cut(s) 2007, 2039
PstI CTGCAG 1 cut(s) 2185
PstNI CAGNNNCTG 2 cut(s) 178, 3935
PsuI RGATCY 3 cut(s) 643, 1214, 3504
PsyI GACNNNGTC 1 cut(s) 2206
PvuII CAGCTG 4 cut(s) 1817, 2225, 3455, 3683
RruI TCGCGA 1 cut(s) 1330
RseI CAYNNNNRTG 3 cut(s) 2372, 2780, 3518
SacI GAGCTC 2 cut(s) 57, 3358
SapI GCTCTTC 1 cut(s) 2550
ScaI AGTACT 3 cut(s) 930, 1133, 3124
SduI GDGCHC 8 cut(s) 57, 717, 990, 1500, 1740, 2065, 3358, 3872
SfaNI GCATC 4 cut(s) 646, 680, 1781, 3000
SfcI CTRYAG 3 cut(s) 798, 924, 2181
Sfr274I CTCGAG 1 cut(s) 1023
SfuI TTCGAA 1 cut(s) 1148
SinI GGWCC 4 cut(s) 362, 818, 1377, 3854
SlaI CTCGAG 1 cut(s) 1023
SmiMI CAYNNNNRTG 3 cut(s) 2372, 2780, 3518
SmlI CTYRAG 3 cut(s) 62, 1023, 2505
SmoI CTYRAG 3 cut(s) 62, 1023, 2505
SpeI ACTAGT 2 cut(s) 266, 2608
SphI GCATGC 2 cut(s) 1323, 2636
SsiI CCGC 8 cut(s) 5, 49, 324, 1088, 2988, 3000, 3485, 3647
SspI AATATT 1 cut(s) 652
SstI GAGCTC 2 cut(s) 57, 3358
StyI CCWWGG 3 cut(s) 546, 1877, 3898
TaiI ACGT 7 cut(s) 27, 198, 1008, 1421, 2382, 2455, 3957
TaqI TCGA 8 cut(s) 21, 129, 1024, 1148, 2592, 2850, 3419, 3442
TaqII GACCGA 1 cut(s) 707
TatI WGTACW 4 cut(s) 928, 1131, 3087, 3122
TauI GCSGC 4 cut(s) 8, 327, 3488, 3650
TfiI GAWTC 8 cut(s) 31, 175, 1059, 1848, 2970, 2980, 3214, 3715
TscAI CASTG 5 cut(s) 85, 1773, 2103, 2151, 3342
TseFI GTSAC 5 cut(s) 1001, 1405, 2098, 2328, 3658
Tsp45I GTSAC 5 cut(s) 1001, 1405, 2098, 2328, 3658
TspGWI ACGGA 1 cut(s) 1700
TspRI CASTG 5 cut(s) 85, 1773, 2103, 2151, 3342
Tth111I GACNNNGTC 1 cut(s) 2206
Van91I CCANNNNNTGG 1 cut(s) 374
VneI GTGCAC 2 cut(s) 1496, 1736
VpaK11BI GGWCC 4 cut(s) 362, 818, 1377, 3854
VspI ATTAAT 1 cut(s) 2964
XagI CCTNNNNNAGG 2 cut(s) 65, 2555
XapI RAATTY 8 cut(s) 687, 901, 1609, 1703, 1943, 3076, 3584, 3989
XceI RCATGY 3 cut(s) 1323, 1413, 2636
XcmI CCANNNNNNNNNTGG 2 cut(s) 1884, 2752
XhoI CTCGAG 1 cut(s) 1023
XmiI GTMKAC 2 cut(s) 1668, 3534
XmnI GAANNNNTTC 3 cut(s) 1706, 3463, 3719
ZraI GACGTC 2 cut(s) 25, 2380
ZrmI AGTACT 3 cut(s) 930, 1133, 3124
Zsp2I ATGCAT 1 cut(s) 2080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.