Rh5DG035400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004788
Physical Location & Seq
Reverse (-)
2499602 .. 2501485
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG035400.1

Sequence Viewer

Length: 612 bp
ATGGCGGAAATTCCCAAGTTGGATTTGGCTTCCCTTGATGAGGTTTATGGATTAGAAGAGAAAATGGGCACCCAGAAAATCTCAGTTTCGGATCATATAAATGGATTTCACTACACAGCTGATAAATCTGATAGCTTTGTGATTGACATGGAGAGTTTCAACCATGGCGTCACAGATAAAGATGTAACTGCAAATTCTAGAATCTCGTTGCAGAGAAATCTTTCAAGGAAATGGTCACAGAGAGTTGGAGAGAAGAAGATAAGCACCAATGGTAATTCAAATGATAGAGATATTTCAGGTTCAGCTATAACTTCACCCAAAGGTCCTATTACGCCCGAAAAGCCAGCGGTGGTTGCAGGGACTGCAGATCACCCGGGCAGCCCACAAGTCCACCATCAGATTACCGTCACAGCGGCCAACATTGGCACCACCACCGATGGCAGATGCGTTGCTAGGAGAAATAGTTTCCGGCGTTCTTCATCGTGGGCCGTTGATCCCAAGAGGGTTCTCTTCTTCTTTGCAACATTGTCAAGCATTGGTACAATCTTATTGATATACTTCACACTTACAATTGGCAAGTACAATATAGAGGAAAATAGTCTTGATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

22.33

Weight (kDa)

7.02

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 68, 425
AciI CCGC 3 cut(s) 5, 347, 413
AclWI GGATC 2 cut(s) 99, 488
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 2 cut(s) 9, 193
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 541, 581
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 3 cut(s) 160, 225, 279
AluBI AGCT 3 cut(s) 119, 135, 305
AluI AGCT 3 cut(s) 119, 135, 305
AlwI GGATC 2 cut(s) 99, 488
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 2 cut(s) 414, 486
ApeKI GCWGC 1 cut(s) 378
ApoI RAATTY 2 cut(s) 9, 193
Asp700I GAANNNNTTC 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 323, 486
AsuC2I CCSGG 2 cut(s) 374, 375
AsuHPI GGTGA 2 cut(s) 306, 362
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 71
BanI GGYRCC 2 cut(s) 68, 425
BbvI GCAGC 1 cut(s) 390
BccI CCATC 2 cut(s) 402, 431
BceAI ACGGC 1 cut(s) 473
BcnI CCSGG 2 cut(s) 374, 375
BfaI CTAG 2 cut(s) 198, 453
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 2 cut(s) 379, 414
BlsI GCNGC 2 cut(s) 380, 415
Bme1390I CCNGG 2 cut(s) 374, 375
Bme18I GGWCC 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 323, 486
BmiI GGNNCC 2 cut(s) 70, 427
BmrFI CCNGG 2 cut(s) 374, 375
BmsI GCATC 1 cut(s) 434
BpuMI CCSGG 2 cut(s) 374, 375
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 163, 373
BsaXI ACNNNNNCTCC 2 cut(s) 448, 478
Bsc4I CCNNNNNNNGG 1 cut(s) 40
BseDI CCNNGG 2 cut(s) 163, 373
BseLI CCNNNNNNNGG 1 cut(s) 40
BseMII CTCAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 1 cut(s) 390
BshFI GGCC 2 cut(s) 416, 488
BshNI GGYRCC 2 cut(s) 68, 425
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 2 cut(s) 374, 469
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 2 cut(s) 416, 488
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 3 cut(s) 91, 367, 493
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 3 cut(s) 5, 347, 413
BspANI GGCC 2 cut(s) 416, 488
BspCNI CTCAG 1 cut(s) 95
BspLI GGNNCC 2 cut(s) 70, 427
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 2 cut(s) 99, 488
BspT107I GGYRCC 2 cut(s) 68, 425
BssECI CCNNGG 2 cut(s) 163, 373
BssMI GATC 3 cut(s) 91, 367, 493
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 163
Bst4CI ACNGT 1 cut(s) 406
Bst6I CTCTTC 2 cut(s) 51, 515
BstACI GRCGYC 1 cut(s) 168
BstAPI GCANNNNNTGC 1 cut(s) 362
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 1 cut(s) 82
BstDSI CCRYGG 1 cut(s) 163
BstENI CCTNNNNNAGG 1 cut(s) 38
BstKTI GATC 3 cut(s) 94, 370, 496
BstMBI GATC 3 cut(s) 91, 367, 493
BstMWI GCNNNNNNNGC 3 cut(s) 340, 353, 362
BstSCI CCNGG 2 cut(s) 372, 373
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 390
BsuRI GGCC 2 cut(s) 416, 488
BtgI CCRYGG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 362
Cfr13I GGNCC 2 cut(s) 323, 486
Cfr9I CCCGGG 1 cut(s) 373
CseI GACGC 1 cut(s) 157
Csp6I GTAC 2 cut(s) 540, 580
CviAII CATG 2 cut(s) 148, 164
CviJI RGCY 8 cut(s) 29, 119, 135, 305, 343, 381, 416, 488
CviKI_1 RGCY 8 cut(s) 29, 119, 135, 305, 343, 381, 416, 488
CviQI GTAC 2 cut(s) 540, 580
DdeI CTNAG 1 cut(s) 82
DpnI GATC 3 cut(s) 93, 369, 495
DpnII GATC 3 cut(s) 91, 367, 493
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 51, 515
EarI CTCTTC 2 cut(s) 51, 515
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 2 cut(s) 151, 167
FaiI YATR 8 cut(s) 48, 96, 98, 149, 165, 308, 556, 587
FaqI GGGAC 1 cut(s) 373
FatI CATG 2 cut(s) 147, 163
Fnu4HI GCNGC 2 cut(s) 379, 414
Fsp4HI GCNGC 2 cut(s) 379, 414
FspBI CTAG 2 cut(s) 198, 453
GluI GCNGC 2 cut(s) 379, 414
HaeIII GGCC 2 cut(s) 416, 488
HapII CCGG 2 cut(s) 374, 469
HgaI GACGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 2 cut(s) 151, 167
HinfI GANTC 1 cut(s) 201
HpaII CCGG 2 cut(s) 374, 469
HphI GGTGA 2 cut(s) 306, 362
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 3 cut(s) 91, 130, 399
Hpy188III TCNNGA 2 cut(s) 198, 602
Hpy8I GTNNAC 1 cut(s) 391
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4V TGCA 5 cut(s) 191, 211, 356, 365, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 340, 353, 362
HpyF3I CTNAG 1 cut(s) 82
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 2 cut(s) 151, 167
Kzo9I GATC 3 cut(s) 91, 367, 493
LpnPI CCDG 6 cut(s) 86, 282, 342, 357, 387, 482
Lsp1109I GCAGC 1 cut(s) 390
LweI GCATC 1 cut(s) 434
MaeI CTAG 2 cut(s) 198, 453
MaeIII GTNAC 4 cut(s) 169, 184, 234, 406
MalI GATC 3 cut(s) 93, 369, 495
MboI GATC 3 cut(s) 91, 367, 493
MboII GAAGA 6 cut(s) 68, 265, 268, 468, 502, 505
MfeI CAATTG 1 cut(s) 570
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 4 cut(s) 9, 193, 274, 570
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 3 cut(s) 34, 495, 583
MroXI GAANNNNTTC 1 cut(s) 220
MslI CAYNNNNRTG 1 cut(s) 99
MspA1I CMGCKG 3 cut(s) 119, 347, 413
MspI CCGG 2 cut(s) 374, 469
MspR9I CCNGG 2 cut(s) 374, 375
MunI CAATTG 1 cut(s) 570
MwoI GCNNNNNNNGC 3 cut(s) 340, 353, 362
NciI CCSGG 2 cut(s) 374, 375
NcoI CCATGG 1 cut(s) 163
NdeII GATC 3 cut(s) 91, 367, 493
NlaIII CATG 2 cut(s) 151, 167
NlaIV GGNNCC 2 cut(s) 70, 427
NmuCI GTSAC 3 cut(s) 169, 234, 406
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 2 cut(s) 380, 415
PpuMI RGGWCCY 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 323
PspN4I GGNNCC 2 cut(s) 70, 427
PspPI GGNCC 2 cut(s) 323, 486
PspPPI RGGWCCY 1 cut(s) 323
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 2 cut(s) 541, 581
RsaNI GTAC 2 cut(s) 540, 580
RseI CAYNNNNRTG 1 cut(s) 99
SatI GCNGC 2 cut(s) 379, 414
Sau3AI GATC 3 cut(s) 91, 367, 493
Sau96I GGNCC 2 cut(s) 323, 486
ScrFI CCNGG 2 cut(s) 374, 375
SduI GDGCHC 1 cut(s) 71
SetI ASST 6 cut(s) 45, 121, 137, 301, 307, 325
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 1 cut(s) 323
SmaI CCCGGG 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 99
Sse9I AATT 4 cut(s) 9, 193, 274, 570
SsiI CCGC 3 cut(s) 5, 347, 413
SspMI CTAG 2 cut(s) 198, 453
StyD4I CCNGG 2 cut(s) 372, 373
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 406
TasI AATT 4 cut(s) 9, 193, 274, 570
TatI WGTACW 1 cut(s) 579
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 1 cut(s) 201
TseFI GTSAC 3 cut(s) 169, 234, 406
TseI GCWGC 1 cut(s) 378
Tsp45I GTSAC 3 cut(s) 169, 234, 406
TspDTI ATGAA 1 cut(s) 468
TspMI CCCGGG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 323
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 2 cut(s) 9, 193
XbaI TCTAGA 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XmaI CCCGGG 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 220
XspI CTAG 2 cut(s) 198, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.