Rorug05G0241700

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007136
Physical Location & Seq
Reverse (-)
25773269 .. 25779215
5947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0241700.1

Sequence Viewer

Length: 2628 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTTTCGTCTCCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACTCCGACGACGACGCCGCCGCCGCCGTCTCTTCTTCTTCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCATCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTGTCGCGCGCCAAGCCCGACCCGGTGGACCGCTCGGCGTCGTCGGATCATATGACCAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTGCGGTTCAAATCGGACGGCGTCGAACACTTTAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCCGAGGCCGAGACCAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCTCCGTCGCCGCCGAATAGGCCGCCGTCCGGCCGGCGGTGCGGCGACGAGATTCGGTTAGAGGTGGAGGAGCAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGCGGTGGCGGTGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGCGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATCAAGAGTGTTGCGAGTACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACGTCGTCGGAGAAGGGGGGCAGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGTCGTTTCATGGGCCCGAGAGAGTGAGGGTCAGGCAGTATGGCAAGTCTTGTAAGGAGCTAACGGCTGTGTACAAGACGCAGGAGATTCAGGCGCATCATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGCAAGTATCTGGCGAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGATACCGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTGGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTTTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTCGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGTACCTGCTCTCATCTTCTATGGATAAAACAGTGCGCCTGTGGGATCTGTCTAAAAAGTCTTGTTTGTGGACGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGCATATGGAGCATCCATGATCACCAAGTGGTTGATTGGAATGATACGCATGAGATGGTAACTGCTGCTTGCTACACTCCTGATGGTCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAATGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCAGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTATCGATGGTATTGATCTAGTTCACAAGTTCAAGGGATTTCGTAACACAAATAGCCAAATCTCAGCAACCCTCACAGCAAACGGTAAATATGTGGTATCTGCCAGCGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCGCGCCCTAGCAGAAGCAAAAGTGTTACTGTTACACGGTCGTATGAGCATTTTCATAGTCACAATGTATCAGTGGCCGTCTCTTGGCCTGGTGTTGGTGATTCATGGGGACTACAAGATGGAGATCAAAATGGACTTGGACTTGACAACAATCTTGATGAGGTTTCTACAGCCAATCATCCCCCTACTCCTGTTGAGGAGGCTAACGGCAAGGATGGTTCACGATCAGCATCGGGTTGCACCAATAGCCCTCTCCATGGAACAATTACTAGTGCATCCAACAGTTACTTCTTTGATAGAATTTCGGCAACGTGGCCTGAGGAAAAACTTCCTCTAGCTACTAAGAAACGAAGCCCTCGTGTAAGTTTTGATTTTAGCAATGGTGTAAGCCAGAACATTCCGGCCTGGGGTATGGTGATTGTAACCGCTGGCTTGGGAGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATTTAA

Protein Analysis

875

Amino Acids

96.22

Weight (kDa)

6.28

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 384 - 425 1.8e-07 THOC3 beta-propeller domain
WD40 PF00400 388 - 421 2.6e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 390 - 431 1.9e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 495 - 576 2e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 496 - 622 3.6e-15 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 497 - 713 1.1e-35 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 497 - 559 1.7e-07 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 497 - 533 1.5e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 498 - 597 2.5e-12 CDC20/Fizzy WD40 domain
EIF3I PF24805 498 - 698 9.3e-10 EIF3I
Beta-prop_THOC3 PF25174 501 - 544 1e-06 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 502 - 558 4.1e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_IFT122_1st PF23381 502 - 619 1.8e-06 IFT122 first beta-propeller
Beta-prop_Aladin PF25460 504 - 622 5e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 505 - 711 7.7e-23 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 508 - 709 9.1e-12 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 519 - 623 3.7e-09 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 520 - 709 9.8e-09 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 548 - 717 7.5e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 559 - 720 2.3e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 579 - 711 2.5e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1255
AatII GACGTC 2 cut(s) 222, 1421
Acc36I ACCTGC 2 cut(s) 1255, 1566
AccBSI CCGCTC 2 cut(s) 333, 558
AccII CGCG 4 cut(s) 273, 307, 309, 2146
AccIII TCCGGA 1 cut(s) 382
AclWI GGATC 5 cut(s) 233, 354, 1357, 1605, 1702
AcoI YGGCCR 3 cut(s) 486, 607, 2217
AcsI RAATTY 3 cut(s) 505, 2442, 2602
AcuI CTGAAG 1 cut(s) 1338
AcyI GRCGYC 5 cut(s) 126, 219, 338, 411, 1418
AdeI CACNNNGTG 1 cut(s) 1741
AfaI GTAC 6 cut(s) 215, 679, 979, 1154, 1556, 1965
AfeI AGCGCT 1 cut(s) 176
AfiI CCNNNNNNNGG 9 cut(s) 202, 277, 605, 612, 1827, 2226, 2237, 2399, 2549
AflII CTTAAG 1 cut(s) 752
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 4 cut(s) 400, 937, 955, 2029
AhdI GACNNNNNGTC 1 cut(s) 1027
AhlI ACTAGT 2 cut(s) 1966, 2411
AjiI CACGTC 1 cut(s) 1026
AjnI CCWGG 4 cut(s) 1074, 1945, 2230, 2546
AjuI GAANNNNNNNTTGG 2 cut(s) 2414, 2446
AluBI AGCT 7 cut(s) 751, 1056, 1141, 1209, 1833, 2138, 2480
AluI AGCT 7 cut(s) 751, 1056, 1141, 1209, 1833, 2138, 2480
Alw21I GWGCWC 1 cut(s) 1810
Alw26I GTCTC 8 cut(s) 73, 145, 269, 491, 1375, 1460, 1554, 2227
AlwI GGATC 5 cut(s) 233, 354, 1357, 1605, 1702
AlwNI CAGNNNCTG 2 cut(s) 832, 1558
Ama87I CYCGRG 1 cut(s) 1097
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 176
ApaI GGGCCC 1 cut(s) 1098
ApeKI GCWGC 3 cut(s) 431, 1778, 1833
ApoI RAATTY 3 cut(s) 505, 2442, 2602
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
Asp700I GAANNNNTTC 1 cut(s) 2469
AspLEI GCGC 7 cut(s) 177, 309, 311, 431, 1177, 1590, 2148
AsuC2I CCSGG 4 cut(s) 13, 296, 323, 769
AsuHPI GGTGA 8 cut(s) 727, 1250, 1279, 1301, 1727, 2252, 2569, 2594
AsuII TTCGAA 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 1097
AvaII GGWCC 4 cut(s) 328, 572, 765, 1542
AxyI CCTNAGG 1 cut(s) 2460
BaeGI GKGCMC 1 cut(s) 1098
BanII GRGCYC 2 cut(s) 237, 1098
BauI CACGAG 2 cut(s) 1986, 2499
BbsI GAAGAC 2 cut(s) 30, 36
Bbv12I GWGCWC 1 cut(s) 1810
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 418, 1765, 1820
BceAI ACGGC 8 cut(s) 122, 362, 424, 442, 586, 1161, 2204, 2365
BciT130I CCWGG 4 cut(s) 1076, 1947, 2232, 2548
BciVI GTATCC 1 cut(s) 1267
BclI TGATCA 1 cut(s) 1732
BcnI CCSGG 4 cut(s) 13, 296, 323, 769
BcoDI GTCTC 8 cut(s) 73, 145, 269, 491, 1375, 1460, 1554, 2227
BcuI ACTAGT 2 cut(s) 1966, 2411
BfaI CTAG 7 cut(s) 441, 1811, 1967, 2015, 2151, 2412, 2477
BfmI CTRYAG 3 cut(s) 1889, 1977, 2310
BfoI RGCGCY 2 cut(s) 178, 432
BfrI CTTAAG 1 cut(s) 752
BfuAI ACCTGC 2 cut(s) 1255, 1566
BfuI GTATCC 1 cut(s) 1267
BglI GCCNNNNNGGC 2 cut(s) 288, 595
BmcAI AGTACT 2 cut(s) 979, 1965
Bme1390I CCNGG 8 cut(s) 13, 296, 323, 769, 1076, 1947, 2232, 2548
Bme18I GGWCC 4 cut(s) 328, 572, 765, 1542
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmeT110I CYCGRG 1 cut(s) 1097
BmgBI CACGTC 1 cut(s) 1026
BmiI GGNNCC 6 cut(s) 205, 477, 483, 766, 1096, 1819
BmrFI CCNGG 8 cut(s) 13, 296, 323, 769, 1076, 1947, 2232, 2548
BmrI ACTGGG 1 cut(s) 996
BmuI ACTGGG 1 cut(s) 996
BpiI GAAGAC 2 cut(s) 30, 36
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu10I CCTNAGC 2 cut(s) 284, 1707
Bpu14I TTCGAA 1 cut(s) 899
BpuMI CCSGG 4 cut(s) 13, 296, 323, 769
Bsa29I ATCGAT 1 cut(s) 2001
BsaBI GATNNNNATC 2 cut(s) 2265, 2371
BsaHI GRCGYC 5 cut(s) 126, 219, 338, 411, 1418
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 6 cut(s) 12, 489, 783, 1507, 2398, 2547
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 9 cut(s) 202, 277, 605, 612, 1827, 2226, 2237, 2399, 2549
Bse118I RCCGGY 4 cut(s) 609, 869, 1235, 1836
Bse1I ACTGG 4 cut(s) 991, 1657, 1933, 1936
Bse21I CCTNAGG 1 cut(s) 2460
Bse3DI GCAATG 2 cut(s) 574, 2527
Bse8I GATNNNNATC 2 cut(s) 2265, 2371
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 4 cut(s) 1076, 1947, 2232, 2548
BseCI ATCGAT 1 cut(s) 2001
BseDI CCNNGG 6 cut(s) 12, 489, 783, 1507, 2398, 2547
BseGI GGATG 9 cut(s) 434, 744, 1084, 1219, 1653, 1725, 2320, 2362, 2417
BseJI GATNNNNATC 2 cut(s) 2265, 2371
BseLI CCNNNNNNNGG 9 cut(s) 202, 277, 605, 612, 1827, 2226, 2237, 2399, 2549
BseMI GCAATG 2 cut(s) 574, 2527
BseMII CTCAG 5 cut(s) 298, 1565, 1971, 2073, 2451
BseNI ACTGG 4 cut(s) 991, 1657, 1933, 1936
BsePI GCGCGC 1 cut(s) 307
BseSI GKGCMC 1 cut(s) 1098
BseX3I CGGCCG 2 cut(s) 486, 607
BseXI GCAGC 3 cut(s) 418, 1765, 1820
Bsh1236I CGCG 4 cut(s) 273, 307, 309, 2146
Bsh1285I CGRYCG 5 cut(s) 58, 253, 489, 610, 2183
BshVI ATCGAT 1 cut(s) 2001
BsiEI CGRYCG 5 cut(s) 58, 253, 489, 610, 2183
BsiHKAI GWGCWC 1 cut(s) 1810
BsiHKCI CYCGRG 1 cut(s) 1097
BslFI GGGAC 4 cut(s) 34, 778, 1034, 2265
BslI CCNNNNNNNGG 9 cut(s) 202, 277, 605, 612, 1827, 2226, 2237, 2399, 2549
BsmAI GTCTC 8 cut(s) 73, 145, 269, 491, 1375, 1460, 1554, 2227
BsmBI CGTCTC 5 cut(s) 73, 145, 269, 1375, 2227
BsmFI GGGAC 4 cut(s) 34, 778, 1034, 2265
BsmI GAATGC 1 cut(s) 907
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 1 cut(s) 1097
Bsp119I TTCGAA 1 cut(s) 899
Bsp120I GGGCCC 1 cut(s) 1094
Bsp1286I GDGCHC 3 cut(s) 237, 1098, 1810
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 1152
Bsp19I CCATGG 2 cut(s) 783, 2398
BspCNI CTCAG 5 cut(s) 297, 1564, 1970, 2072, 2452
BspDI ATCGAT 1 cut(s) 2001
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 4 cut(s) 273, 307, 309, 2146
BspLI GGNNCC 6 cut(s) 205, 477, 483, 766, 1096, 1819
BspMAI CTGCAG 2 cut(s) 1893, 1981
BspMI ACCTGC 2 cut(s) 1255, 1566
BspPI GGATC 5 cut(s) 233, 354, 1357, 1605, 1702
BspT104I TTCGAA 1 cut(s) 899
BspTI CTTAAG 1 cut(s) 752
BspTNI GGTCTC 1 cut(s) 491
BsrBI CCGCTC 2 cut(s) 333, 558
BsrDI GCAATG 2 cut(s) 574, 2527
BsrFI RCCGGY 4 cut(s) 609, 869, 1235, 1836
BsrGI TGTACA 1 cut(s) 1152
BsrI ACTGG 4 cut(s) 991, 1657, 1933, 1936
BssAI RCCGGY 4 cut(s) 609, 869, 1235, 1836
BssECI CCNNGG 6 cut(s) 12, 489, 783, 1507, 2398, 2547
BssHII GCGCGC 1 cut(s) 307
BssNI GRCGYC 5 cut(s) 126, 219, 338, 411, 1418
BssSI CACGAG 2 cut(s) 1986, 2499
BssT1I CCWWGG 3 cut(s) 783, 1507, 2398
Bst2BI CACGAG 2 cut(s) 1986, 2499
Bst2UI CCWGG 4 cut(s) 1076, 1947, 2232, 2548
Bst4CI ACNGT 6 cut(s) 982, 1585, 2081, 2173, 2181, 2426
Bst6I CTCTTC 3 cut(s) 148, 935, 1400
BstACI GRCGYC 5 cut(s) 126, 219, 338, 411, 1418
BstAFI CTTAAG 1 cut(s) 752
BstAUI TGTACA 1 cut(s) 1152
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 9 cut(s) 173, 237, 309, 556, 611, 871, 1783, 2101, 2572
BstDEI CTNAG 7 cut(s) 284, 1551, 1707, 1957, 2059, 2460, 2484
BstDSI CCRYGG 2 cut(s) 783, 2398
BstEII GGTNACC 1 cut(s) 1289
BstF5I GGATG 9 cut(s) 434, 744, 1084, 1219, 1653, 1725, 2320, 2362, 2417
BstFNI CGCG 4 cut(s) 273, 307, 309, 2146
BstH2I RGCGCY 2 cut(s) 178, 432
BstHHI GCGC 7 cut(s) 177, 309, 311, 431, 1177, 1590, 2148
BstMAI GTCTC 8 cut(s) 73, 145, 269, 491, 1375, 1460, 1554, 2227
BstMCI CGRYCG 5 cut(s) 58, 253, 489, 610, 2183
BstNI CCWGG 4 cut(s) 1076, 1947, 2232, 2548
BstPI GGTNACC 1 cut(s) 1289
BstSCI CCNGG 8 cut(s) 11, 294, 321, 767, 1074, 1945, 2230, 2546
BstSFI CTRYAG 3 cut(s) 1889, 1977, 2310
BstSLI GKGCMC 1 cut(s) 1098
BstUI CGCG 4 cut(s) 273, 307, 309, 2146
BstV1I GCAGC 3 cut(s) 418, 1765, 1820
BstV2I GAAGAC 2 cut(s) 30, 36
BstX2I RGATCY 3 cut(s) 225, 1349, 1597
BstXI CCANNNNNNTGG 1 cut(s) 2606
BstYI RGATCY 3 cut(s) 225, 1349, 1597
BstZI CGGCCG 2 cut(s) 486, 607
Bsu15I ATCGAT 1 cut(s) 2001
Bsu36I CCTNAGG 1 cut(s) 2460
BsuI GTATCC 1 cut(s) 1267
BsuTUI ATCGAT 1 cut(s) 2001
BtgI CCRYGG 2 cut(s) 783, 2398
BtrI CACGTC 1 cut(s) 1026
BtsCI GGATG 9 cut(s) 434, 744, 1084, 1219, 1653, 1725, 2320, 2362, 2417
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 4 cut(s) 1015, 1062, 1590, 2220
BveI ACCTGC 2 cut(s) 1255, 1566
Cac8I GCNNGC 9 cut(s) 173, 237, 309, 556, 611, 871, 1783, 2101, 2572
CaiI CAGNNNCTG 2 cut(s) 832, 1558
CfoI GCGC 7 cut(s) 177, 309, 311, 431, 1177, 1590, 2148
Cfr10I RCCGGY 4 cut(s) 609, 869, 1235, 1836
ClaI ATCGAT 1 cut(s) 2001
CseI GACGC 4 cut(s) 134, 327, 400, 1168
Csp6I GTAC 6 cut(s) 214, 678, 978, 1153, 1555, 1964
CviQI GTAC 6 cut(s) 214, 678, 978, 1153, 1555, 1964
DdeI CTNAG 7 cut(s) 284, 1551, 1707, 1957, 2059, 2460, 2484
DraIII CACNNNGTG 1 cut(s) 1741
DriI GACNNNNNGTC 1 cut(s) 1027
EaeI YGGCCR 3 cut(s) 486, 607, 2217
EagI CGGCCG 2 cut(s) 486, 607
Eam1104I CTCTTC 3 cut(s) 148, 935, 1400
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EarI CTCTTC 3 cut(s) 148, 935, 1400
EclXI CGGCCG 2 cut(s) 486, 607
Eco130I CCWWGG 3 cut(s) 783, 1507, 2398
Eco24I GRGCYC 2 cut(s) 237, 1098
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 4 cut(s) 328, 572, 765, 1542
Eco47III AGCGCT 1 cut(s) 176
Eco52I CGGCCG 2 cut(s) 486, 607
Eco57I CTGAAG 1 cut(s) 1338
Eco81I CCTNAGG 1 cut(s) 2460
Eco88I CYCGRG 1 cut(s) 1097
Eco91I GGTNACC 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 1289
EcoRII CCWGG 4 cut(s) 1074, 1945, 2230, 2546
EcoT14I CCWWGG 3 cut(s) 783, 1507, 2398
EcoT38I GRGCYC 2 cut(s) 237, 1098
ErhI CCWWGG 3 cut(s) 783, 1507, 2398
Esp3I CGTCTC 5 cut(s) 73, 145, 269, 1375, 2227
FalI AAGNNNNNCTT 2 cut(s) 1193, 1225
FaqI GGGAC 4 cut(s) 34, 778, 1034, 2265
FauI CCCGC 3 cut(s) 176, 244, 914
FauNDI CATATG 2 cut(s) 351, 1718
FbaI TGATCA 1 cut(s) 1732
FokI GGATG 9 cut(s) 421, 751, 1091, 1226, 1640, 1712, 2307, 2369, 2404
FriOI GRGCYC 2 cut(s) 237, 1098
FspBI CTAG 7 cut(s) 441, 1811, 1967, 2015, 2151, 2412, 2477
GlaI GCGC 7 cut(s) 176, 308, 310, 430, 1176, 1589, 2147
HaeII RGCGCY 2 cut(s) 178, 432
HgaI GACGC 4 cut(s) 134, 327, 400, 1168
HhaI GCGC 7 cut(s) 177, 309, 311, 431, 1177, 1590, 2148
Hin1I GRCGYC 5 cut(s) 126, 219, 338, 411, 1418
Hin6I GCGC 7 cut(s) 175, 307, 309, 429, 1175, 1588, 2146
HinP1I GCGC 7 cut(s) 175, 307, 309, 429, 1175, 1588, 2146
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HindIII AAGCTT 1 cut(s) 749
HphI GGTGA 8 cut(s) 727, 1250, 1279, 1301, 1727, 2252, 2569, 2594
Hpy166II GTNNAC 7 cut(s) 328, 678, 780, 1153, 1623, 2020, 2363
Hpy8I GTNNAC 7 cut(s) 328, 678, 780, 1153, 1623, 2020, 2363
HpyAV CCTTC 5 cut(s) 585, 697, 996, 1030, 1279
HpyCH4III ACNGT 6 cut(s) 982, 1585, 2081, 2173, 2181, 2426
HpyCH4IV ACGT 6 cut(s) 219, 262, 1025, 1418, 1625, 2453
HpyF3I CTNAG 7 cut(s) 284, 1551, 1707, 1957, 2059, 2460, 2484
HpySE526I ACGT 6 cut(s) 219, 262, 1025, 1418, 1625, 2453
Hsp92I GRCGYC 5 cut(s) 126, 219, 338, 411, 1418
HspAI GCGC 7 cut(s) 175, 307, 309, 429, 1175, 1588, 2146
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 2 cut(s) 609, 869
KroNI GCCGGC 2 cut(s) 611, 871
Ksp22I TGATCA 1 cut(s) 1732
LmnI GCTCC 8 cut(s) 101, 481, 487, 646, 1005, 1138, 1722, 1830
Lsp1109I GCAGC 3 cut(s) 418, 1765, 1820
MaeI CTAG 7 cut(s) 441, 1811, 1967, 2015, 2151, 2412, 2477
MaeII ACGT 6 cut(s) 219, 262, 1025, 1418, 1625, 2453
MbiI CCGCTC 2 cut(s) 333, 558
MflI RGATCY 3 cut(s) 225, 1349, 1597
MhlI GDGCHC 3 cut(s) 237, 1098, 1810
MluCI AATT 8 cut(s) 157, 505, 1321, 1923, 2406, 2442, 2586, 2602
MlyI GAGTC 4 cut(s) 56, 107, 868, 1998
MmeI TCCRAC 8 cut(s) 135, 141, 273, 324, 1011, 1409, 1795, 2445
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 2 cut(s) 609, 869
MroXI GAANNNNTTC 1 cut(s) 2469
MseI TTAA 5 cut(s) 423, 753, 1200, 1662, 2626
MslI CAYNNNNRTG 1 cut(s) 1518
MspA1I CMGCKG 2 cut(s) 288, 2570
MspCI CTTAAG 1 cut(s) 752
MspR9I CCNGG 8 cut(s) 13, 296, 323, 769, 1076, 1947, 2232, 2548
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 4 cut(s) 1076, 1947, 2232, 2548
MvnI CGCG 4 cut(s) 273, 307, 309, 2146
NaeI GCCGGC 2 cut(s) 611, 871
NciI CCSGG 4 cut(s) 13, 296, 323, 769
NcoI CCATGG 2 cut(s) 783, 2398
NdeI CATATG 2 cut(s) 351, 1718
NgoMIV GCCGGC 2 cut(s) 609, 869
NlaIV GGNNCC 6 cut(s) 205, 477, 483, 766, 1096, 1819
NmeAIII GCCGAG 3 cut(s) 314, 514, 520
NmuCI GTSAC 4 cut(s) 860, 982, 1289, 2201
NspV TTCGAA 1 cut(s) 899
PaqCI CACCTGC 1 cut(s) 1255
PauI GCGCGC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 2497
PctI GAATGC 1 cut(s) 907
PdiI GCCGGC 2 cut(s) 611, 871
PdmI GAANNNNTTC 1 cut(s) 2469
PflFI GACNNNGTC 1 cut(s) 410
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 4 cut(s) 55, 107, 867, 1997
PpsI GAGTC 4 cut(s) 55, 107, 867, 1997
Psp6I CCWGG 4 cut(s) 1074, 1945, 2230, 2546
PspEI GGTNACC 1 cut(s) 1289
PspGI CCWGG 4 cut(s) 1074, 1945, 2230, 2546
PspN4I GGNNCC 6 cut(s) 205, 477, 483, 766, 1096, 1819
PspOMI GGGCCC 1 cut(s) 1094
PstI CTGCAG 2 cut(s) 1893, 1981
PstNI CAGNNNCTG 2 cut(s) 832, 1558
PsuI RGATCY 3 cut(s) 225, 1349, 1597
PsyI GACNNNGTC 1 cut(s) 410
PteI GCGCGC 1 cut(s) 307
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 6 cut(s) 215, 679, 979, 1154, 1556, 1965
RsaNI GTAC 6 cut(s) 214, 678, 978, 1153, 1555, 1964
RseI CAYNNNNRTG 1 cut(s) 1518
SaqAI TTAA 5 cut(s) 423, 753, 1200, 1662, 2626
ScaI AGTACT 2 cut(s) 979, 1965
SchI GAGTC 4 cut(s) 56, 107, 868, 1998
ScrFI CCNGG 8 cut(s) 13, 296, 323, 769, 1076, 1947, 2232, 2548
SduI GDGCHC 3 cut(s) 237, 1098, 1810
SfcI CTRYAG 3 cut(s) 1889, 1977, 2310
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 4 cut(s) 328, 572, 765, 1542
SmiMI CAYNNNNRTG 1 cut(s) 1518
SmlI CTYRAG 1 cut(s) 752
SmoI CTYRAG 1 cut(s) 752
SpeI ACTAGT 2 cut(s) 1966, 2411
Sse9I AATT 8 cut(s) 157, 505, 1321, 1923, 2406, 2442, 2586, 2602
SspMI CTAG 7 cut(s) 441, 1811, 1967, 2015, 2151, 2412, 2477
StyD4I CCNGG 8 cut(s) 11, 294, 321, 767, 1074, 1945, 2230, 2546
StyI CCWWGG 3 cut(s) 783, 1507, 2398
TaaI ACNGT 6 cut(s) 982, 1585, 2081, 2173, 2181, 2426
TaiI ACGT 6 cut(s) 222, 265, 1028, 1421, 1628, 2456
TaqI TCGA 5 cut(s) 54, 414, 899, 1518, 2001
TasI AATT 8 cut(s) 157, 505, 1321, 1923, 2406, 2442, 2586, 2602
TatI WGTACW 4 cut(s) 677, 977, 1152, 1963
Tru1I TTAA 5 cut(s) 423, 753, 1200, 1662, 2626
Tru9I TTAA 5 cut(s) 423, 753, 1200, 1662, 2626
TscAI CASTG 4 cut(s) 1015, 1069, 1590, 2220
TseFI GTSAC 4 cut(s) 860, 982, 1289, 2201
TseI GCWGC 3 cut(s) 431, 1778, 1833
Tsp45I GTSAC 4 cut(s) 860, 982, 1289, 2201
TspDTI ATGAA 7 cut(s) 1079, 1244, 1491, 1675, 2148, 2186, 2235
TspGWI ACGGA 2 cut(s) 570, 1357
TspRI CASTG 4 cut(s) 1015, 1069, 1590, 2220
Tth111I GACNNNGTC 1 cut(s) 410
Vha464I CTTAAG 1 cut(s) 752
VpaK11BI GGWCC 4 cut(s) 328, 572, 765, 1542
XapI RAATTY 3 cut(s) 505, 2442, 2602
XmnI GAANNNNTTC 1 cut(s) 2469
XspI CTAG 7 cut(s) 441, 1811, 1967, 2015, 2151, 2412, 2477
ZraI GACGTC 2 cut(s) 220, 1419
ZrmI AGTACT 2 cut(s) 979, 1965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.