Rorug05G0522900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007110
Physical Location & Seq
Forward (+)
70837457 .. 70840335
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0522900.1

Sequence Viewer

Length: 1104 bp
ATGGCAACCGCCGAAAAAGTTGAAGACCTCCCAAAGCTGCCGTCGGAGATCATCCGCGGGATTCTTGCGTGGCTGCCGGTGAAGTCGTTATGCCGATTCAGGTGTGTCAAAAAGTCTTGGAATTCTCTAATCCTTGATCCTCACTTTGTGAAGTTGCACTATGACAAAGCCATTGAGCATGAGGATGTGTTCTACCAAAGAAGAAGAGTCTTCATTAGTGATATGTGGACGCTTTACTCTATCGACCTCGATGAGTGTCTCAATCGTATTAATCTTGATGATTATTCTGGGAATTGTGTTTTAGTAGACAATAAATATCTTCAAGATTTTATGGGTTTGGTGATTCATAGCAATGGCTTGTTGTTCATGCGGTCATGTTATCATGAATTTAGTTTGTTTAACCCCGCCATCAGCCAGACCAAGAATTTACCTGTCCCACCAACGTATGATACTTGGTCTGATGAAGTGTGTGGGTTTGGATTTGATCTGTCTACCCTAGATTACAAGGTGGTTCATGGGCTTTTCTGTCAAGATGAACTTAGGTTTAGTGTTTATTCATTGAAAACCGGTTCTTGGCGAGTGATTCAAAGTGGATACCCCTACAAATATCGAACACCGGCGATGGATGGGAGACTGGTGAATGGAGGAGTTCATTGGTTGGTTCGTAGAGTCCATGACAGATCATCTATGGTGATCGTATCTTTTGTTTTAGCAGAGGAGGATGTTCGTGAGATTCCTCTGCCGCCTGATTTTAATATTGTTGAAGGTGAAGATGAATATTATCTCAGCGTCTTCGAGGAATGCTTATGTGTGGGTAAAAAGGATGATCAAGAGTTTTGGGTCATGAAAGAATACGGAGTGGCCAAATCCTGGACTAAAATAAGCATCTCCCTACCCTTTTCTGAAGGGTTACATTCCGGTTTTGTGAAGAAAAATCATCATCTATTGCTGCTGCAAGATCAAATGCAACAGCGGTTGGTTATGTACAACTTTGATAAAAAAAGCTTTCGCAATCTATCAATTCGTGGAATTCGCGAACCTTCTTCTGCTGGGTTTTACCTGGAGAGCCTTTTGTCACCCAATAACTATAGCATCTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

367

Amino Acids

42.72

Weight (kDa)

6.02

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 50 1.8e-07 F-box domain
F-box-like PF12937 11 - 48 1.1e-06 F-box-like
b-prop_At3g26010-like PF24750 86 - 249 2.9e-06 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 106 - 358 3.3e-22 F-box associated beta propeller domain
FBA_3 PF08268 115 - 346 1.8e-17 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 870
AccI GTMKAC 2 cut(s) 306, 491
AccII CGCG 2 cut(s) 57, 1035
AciI CCGC 7 cut(s) 9, 55, 57, 370, 405, 743, 973
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 4 cut(s) 121, 386, 424, 1029
AcuI CTGAAG 1 cut(s) 924
AdeI CACNNNGTG 1 cut(s) 148
AfaI GTAC 1 cut(s) 986
AfiI CCNNNNNNNGG 2 cut(s) 573, 870
AgeI ACCGGT 1 cut(s) 566
AgsI TTSAA 5 cut(s) 23, 323, 562, 587, 764
AjnI CCWGG 2 cut(s) 869, 1059
AluBI AGCT 2 cut(s) 37, 1005
AluI AGCT 2 cut(s) 37, 1005
Alw26I GTCTC 2 cut(s) 263, 625
AlwI GGATC 1 cut(s) 131
AoxI GGCC 1 cut(s) 861
ApeKI GCWGC 4 cut(s) 37, 73, 949, 952
ApoI RAATTY 4 cut(s) 121, 386, 424, 1029
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AseI ATTAAT 1 cut(s) 270
AsiGI ACCGGT 1 cut(s) 566
AsuHPI GGTGA 6 cut(s) 91, 352, 649, 703, 779, 1068
BalI TGGCCA 1 cut(s) 863
BbsI GAAGAC 3 cut(s) 30, 202, 784
BbvI GCAGC 4 cut(s) 24, 60, 936, 939
BccI CCATC 3 cut(s) 416, 616, 620
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 2 cut(s) 871, 1061
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 826
BcoDI GTCTC 2 cut(s) 263, 625
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 5 cut(s) 38, 74, 743, 950, 953
BlsI GCNGC 5 cut(s) 39, 75, 744, 951, 954
Bme1390I CCNGG 2 cut(s) 871, 1061
BmrFI CCNGG 2 cut(s) 871, 1061
BmsI GCATC 1 cut(s) 894
BpiI GAAGAC 3 cut(s) 30, 202, 784
BpmI CTGGAG 1 cut(s) 1082
BsaJI CCNNGG 1 cut(s) 55
BsaWI WCCGGW 2 cut(s) 566, 917
Bsc4I CCNNNNNNNGG 2 cut(s) 573, 870
Bse118I RCCGGY 3 cut(s) 76, 566, 616
Bse1I ACTGG 1 cut(s) 639
Bse3DI GCAATG 1 cut(s) 358
BseBI CCWGG 2 cut(s) 871, 1061
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 5 cut(s) 51, 190, 631, 727, 829
BseLI CCNNNNNNNGG 2 cut(s) 573, 870
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 799
BseNI ACTGG 1 cut(s) 639
BseRI GAGGAG 2 cut(s) 660, 731
BseXI GCAGC 4 cut(s) 24, 60, 936, 939
BseYI CCCAGC 1 cut(s) 1049
Bsh1236I CGCG 2 cut(s) 57, 1035
BshFI GGCC 1 cut(s) 863
BshTI ACCGGT 1 cut(s) 566
BsiSI CCGG 4 cut(s) 77, 567, 617, 918
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 2 cut(s) 573, 870
BsmAI GTCTC 2 cut(s) 263, 625
BsmFI GGGAC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 1 cut(s) 863
Bsp1407I TGTACA 1 cut(s) 984
Bsp143I GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
Bsp68I TCGCGA 1 cut(s) 1035
BspACI CCGC 7 cut(s) 9, 55, 57, 370, 405, 743, 973
BspANI GGCC 1 cut(s) 863
BspCNI CTCAG 1 cut(s) 798
BspFNI CGCG 2 cut(s) 57, 1035
BspHI TCATGA 2 cut(s) 382, 843
BspPI GGATC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 358
BsrFI RCCGGY 3 cut(s) 76, 566, 616
BsrGI TGTACA 1 cut(s) 984
BsrI ACTGG 1 cut(s) 639
BssAI RCCGGY 3 cut(s) 76, 566, 616
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
Bst2UI CCWGG 2 cut(s) 871, 1061
Bst6I CTCTTC 1 cut(s) 199
BstAUI TGTACA 1 cut(s) 984
BstDEI CTNAG 2 cut(s) 539, 785
BstDSI CCRYGG 1 cut(s) 55
BstF5I GGATG 5 cut(s) 51, 190, 631, 727, 829
BstFNI CGCG 2 cut(s) 57, 1035
BstKTI GATC 7 cut(s) 51, 139, 487, 683, 696, 829, 961
BstMAI GTCTC 2 cut(s) 263, 625
BstMBI GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
BstNI CCWGG 2 cut(s) 871, 1061
BstSCI CCNGG 2 cut(s) 869, 1059
BstSFI CTRYAG 1 cut(s) 1087
BstUI CGCG 2 cut(s) 57, 1035
BstV1I GCAGC 4 cut(s) 24, 60, 936, 939
BstV2I GAAGAC 3 cut(s) 30, 202, 784
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 1 cut(s) 863
BtgI CCRYGG 1 cut(s) 55
BtgZI GCGATG 1 cut(s) 635
BtsCI GGATG 5 cut(s) 51, 190, 631, 727, 829
BtuMI TCGCGA 1 cut(s) 1035
CciI TCATGA 2 cut(s) 382, 843
Cfr10I RCCGGY 3 cut(s) 76, 566, 616
Cfr42I CCGCGG 1 cut(s) 58
CseI GACGC 2 cut(s) 238, 778
Csp6I GTAC 1 cut(s) 985
CspAI ACCGGT 1 cut(s) 566
CviAII CATG 7 cut(s) 179, 367, 375, 383, 515, 674, 844
CviJI RGCY 9 cut(s) 37, 73, 170, 357, 414, 520, 863, 1005, 1068
CviKI_1 RGCY 9 cut(s) 37, 73, 170, 357, 414, 520, 863, 1005, 1068
CviQI GTAC 1 cut(s) 985
DdeI CTNAG 2 cut(s) 539, 785
DpnI GATC 7 cut(s) 50, 138, 486, 682, 695, 828, 960
DpnII GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
DraIII CACNNNGTG 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 861
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 924
EcoRI GAATTC 2 cut(s) 121, 1029
EcoRII CCWGG 2 cut(s) 869, 1059
FaeI CATG 7 cut(s) 182, 370, 378, 386, 518, 677, 847
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 1 cut(s) 419
FatI CATG 7 cut(s) 178, 366, 374, 382, 514, 673, 843
FauI CCCGC 2 cut(s) 50, 412
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 2 cut(s) 306, 491
Fnu4HI GCNGC 5 cut(s) 38, 74, 743, 950, 953
FokI GGATG 5 cut(s) 38, 197, 638, 734, 836
Fsp4HI GCNGC 5 cut(s) 38, 74, 743, 950, 953
FspBI CTAG 1 cut(s) 497
GluI GCNGC 5 cut(s) 38, 74, 743, 950, 953
GsaI CCCAGC 1 cut(s) 1053
GsuI CTGGAG 1 cut(s) 1082
HaeIII GGCC 1 cut(s) 863
HapII CCGG 4 cut(s) 77, 567, 617, 918
HgaI GACGC 2 cut(s) 238, 778
Hin1II CATG 7 cut(s) 182, 370, 378, 386, 518, 677, 847
HindIII AAGCTT 1 cut(s) 1003
HinfI GANTC 7 cut(s) 61, 96, 207, 343, 583, 669, 733
HpaII CCGG 4 cut(s) 77, 567, 617, 918
HphI GGTGA 6 cut(s) 91, 352, 649, 703, 779, 1068
Hpy166II GTNNAC 3 cut(s) 228, 307, 492
Hpy188I TCNGA 3 cut(s) 46, 460, 904
Hpy188III TCNNGA 8 cut(s) 275, 323, 383, 530, 728, 830, 844, 1034
Hpy8I GTNNAC 3 cut(s) 228, 307, 492
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 758, 899, 1050
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 3 cut(s) 157, 955, 967
HpyF3I CTNAG 2 cut(s) 539, 785
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 7 cut(s) 182, 370, 378, 386, 518, 677, 847
Ksp22I TGATCA 1 cut(s) 826
KspI CCGCGG 1 cut(s) 58
Kzo9I GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
Lsp1109I GCAGC 4 cut(s) 24, 60, 936, 939
LweI GCATC 1 cut(s) 894
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 909, 1074
MalI GATC 7 cut(s) 50, 138, 486, 682, 695, 828, 960
MboI GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
MboII GAAGA 9 cut(s) 35, 202, 213, 216, 311, 782, 784, 940, 1035
MlsI TGGCCA 1 cut(s) 863
MluCI AATT 6 cut(s) 121, 292, 386, 424, 1020, 1029
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 2 cut(s) 216, 678
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 9 cut(s) 38, 150, 175, 257, 638, 709, 712, 747, 790
Mox20I TGGCCA 1 cut(s) 863
MscI TGGCCA 1 cut(s) 863
MseI TTAA 3 cut(s) 270, 399, 753
MslI CAYNNNNRTG 2 cut(s) 183, 351
Msp20I TGGCCA 1 cut(s) 863
MspA1I CMGCKG 2 cut(s) 57, 973
MspI CCGG 4 cut(s) 77, 567, 617, 918
MspR9I CCNGG 2 cut(s) 871, 1061
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 2 cut(s) 871, 1061
MvnI CGCG 2 cut(s) 57, 1035
NdeII GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
NlaIII CATG 7 cut(s) 182, 370, 378, 386, 518, 677, 847
NmuCI GTSAC 1 cut(s) 1074
NruI TCGCGA 1 cut(s) 1035
PagI TCATGA 2 cut(s) 382, 843
PctI GAATGC 1 cut(s) 806
PfeI GAWTC 5 cut(s) 61, 96, 343, 583, 733
PflMI CCANNNNNTGG 1 cut(s) 870
PfoI TCCNGGA 1 cut(s) 869
PinAI ACCGGT 1 cut(s) 566
PkrI GCNGC 5 cut(s) 39, 75, 744, 951, 954
PleI GAGTC 2 cut(s) 215, 677
PpsI GAGTC 2 cut(s) 215, 677
PshBI ATTAAT 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 869, 1059
PspFI CCCAGC 1 cut(s) 1049
PspGI CCWGG 2 cut(s) 869, 1059
RruI TCGCGA 1 cut(s) 1035
RsaI GTAC 1 cut(s) 986
RsaNI GTAC 1 cut(s) 985
RseI CAYNNNNRTG 2 cut(s) 183, 351
SacII CCGCGG 1 cut(s) 58
SaqAI TTAA 3 cut(s) 270, 399, 753
SatI GCNGC 5 cut(s) 38, 74, 743, 950, 953
Sau3AI GATC 7 cut(s) 48, 136, 484, 680, 693, 826, 958
SchI GAGTC 2 cut(s) 216, 678
ScrFI CCNGG 2 cut(s) 871, 1061
SfaNI GCATC 1 cut(s) 894
SfcI CTRYAG 1 cut(s) 1087
Sfr303I CCGCGG 1 cut(s) 58
SgrAI CRCCGGYG 1 cut(s) 616
SgrBI CCGCGG 1 cut(s) 58
SmiMI CAYNNNNRTG 2 cut(s) 183, 351
Sse9I AATT 6 cut(s) 121, 292, 386, 424, 1020, 1029
SsiI CCGC 7 cut(s) 9, 55, 57, 370, 405, 743, 973
SspI AATATT 2 cut(s) 757, 779
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 869, 1059
TaiI ACGT 1 cut(s) 446
TaqI TCGA 4 cut(s) 243, 249, 610, 795
TasI AATT 6 cut(s) 121, 292, 386, 424, 1020, 1029
TatI WGTACW 1 cut(s) 984
TauI GCSGC 1 cut(s) 745
TfiI GAWTC 5 cut(s) 61, 96, 343, 583, 733
Tru1I TTAA 3 cut(s) 270, 399, 753
Tru9I TTAA 3 cut(s) 270, 399, 753
TseFI GTSAC 1 cut(s) 1074
TseI GCWGC 4 cut(s) 37, 73, 949, 952
Tsp45I GTSAC 1 cut(s) 1074
TspGWI ACGGA 1 cut(s) 870
Van91I CCANNNNNTGG 1 cut(s) 870
VspI ATTAAT 1 cut(s) 270
XapI RAATTY 4 cut(s) 121, 386, 424, 1029
XmiI GTMKAC 2 cut(s) 306, 491
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.