Rorug06G0164400

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017464
Physical Location & Seq
Forward (+)
23396190 .. 23400126
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0164400.1

Sequence Viewer

Length: 534 bp
ATGATGAAAACTGCGATAATCCAATACGCGAGTAACTGCAGTTCAAATAAGGTCTTGAAATTCGGAATCTGGAGAGAGAGAGAGAGAGAGAGAGAAGAACCAGTGAATGAGATGAACCATAACCACCAAAGCCAGCAATCGAGCGAAGGCAGGCACGACGATGACGACGCATCTCTCTCTGAATTCCTCGCCTCCCTAATGGACTACACTCCTACCATACCCGACGAGCTTGTGGAGCATTACTTGGCCAAGAGCGGCTTCCAGTGTCCCGACGTTCGATTAATTAGGCTGGTAGCTGTTGCCACACAAAAGTTTGTCGCTGAGGTTGCAACTGATGCTCTACAGCAATCCAAAGCAAGACAGGCAGCAGTGGTGAAAGACAAAAGGGACAAACAGCAAAAGGATAAGCGCCCAATATTGACTATGGAAGACCTGTCAAAGGCATTGCGTGAGTATGGCGTCAATGTGAAGCACCAAGAATATTTTGCTGACAGCCCTTCAACTGGGATGGATCCTGCATCAAGAGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

177

Amino Acids

20.22

Weight (kDa)

5.61

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF10 PF03540 59 - 162 5.4e-39 Transcription initiation factor TFIID 23-30kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 255
AccII CGCG 1 cut(s) 29
AciI CCGC 1 cut(s) 255
AclWI GGATC 2 cut(s) 506, 519
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 2 cut(s) 59, 182
AcyI GRCGYC 1 cut(s) 459
AfiI CCNNNNNNNGG 2 cut(s) 439, 503
AgsI TTSAA 3 cut(s) 45, 58, 501
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 2 cut(s) 229, 296
AluI AGCT 2 cut(s) 229, 296
AlwI GGATC 2 cut(s) 506, 519
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 2 cut(s) 59, 182
AseI ATTAAT 1 cut(s) 281
AspLEI GCGC 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 385
BalI TGGCCA 1 cut(s) 248
BamHI GGATCC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 435
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 377
BccI CCATC 1 cut(s) 502
BfmI CTRYAG 2 cut(s) 37, 341
BfoI RGCGCY 1 cut(s) 412
BisI GCNGC 2 cut(s) 256, 366
BlsI GCNGC 2 cut(s) 257, 367
BmiI GGNNCC 1 cut(s) 513
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 3 cut(s) 179, 325, 527
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 459
Bsc4I CCNNNNNNNGG 2 cut(s) 439, 503
Bse1I ACTGG 3 cut(s) 101, 262, 508
Bse3DI GCAATG 1 cut(s) 443
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 2 cut(s) 439, 503
BseMI GCAATG 1 cut(s) 443
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 3 cut(s) 101, 262, 508
BseXI GCAGC 1 cut(s) 377
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 248
BslFI GGGAC 2 cut(s) 252, 401
BslI CCNNNNNNNGG 2 cut(s) 439, 503
BsmFI GGGAC 2 cut(s) 252, 401
BsnI GGCC 1 cut(s) 248
Bsp143I GATC 1 cut(s) 511
BspACI CCGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 1 cut(s) 513
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 2 cut(s) 506, 519
BsrBI CCGCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 443
BsrI ACTGG 3 cut(s) 101, 262, 508
BssMI GATC 1 cut(s) 511
BssNI GRCGYC 1 cut(s) 459
BstACI GRCGYC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 134, 152
BstDEI CTNAG 1 cut(s) 321
BstENI CCTNNNNNAGG 1 cut(s) 437
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 1 cut(s) 514
BstMBI GATC 1 cut(s) 511
BstMWI GCNNNNNNNGC 4 cut(s) 235, 326, 335, 362
BstSFI CTRYAG 2 cut(s) 37, 341
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 377
BstV2I GAAGAC 1 cut(s) 435
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuRI GGCC 1 cut(s) 248
BtsCI GGATG 1 cut(s) 513
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 3 cut(s) 108, 269, 375
Cac8I GCNNGC 2 cut(s) 134, 152
CfoI GCGC 1 cut(s) 411
CseI GACGC 2 cut(s) 176, 448
CviJI RGCY 7 cut(s) 132, 229, 248, 258, 289, 296, 495
CviKI_1 RGCY 7 cut(s) 132, 229, 248, 258, 289, 296, 495
DdeI CTNAG 1 cut(s) 321
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 437
EcoRI GAATTC 1 cut(s) 182
FaiI YATR 4 cut(s) 120, 218, 425, 456
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 2 cut(s) 252, 401
Fnu4HI GCNGC 2 cut(s) 256, 366
FokI GGATG 1 cut(s) 520
Fsp4HI GCNGC 2 cut(s) 256, 366
GlaI GCGC 1 cut(s) 410
GluI GCNGC 2 cut(s) 256, 366
GsuI CTGGAG 1 cut(s) 91
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 1 cut(s) 248
HgaI GACGC 2 cut(s) 176, 448
HhaI GCGC 1 cut(s) 411
Hin1I GRCGYC 1 cut(s) 459
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HinfI GANTC 1 cut(s) 66
HphI GGTGA 1 cut(s) 385
Hpy188I TCNGA 2 cut(s) 65, 181
Hpy188III TCNNGA 4 cut(s) 55, 70, 269, 522
Hpy99I CGWCG 4 cut(s) 161, 170, 227, 275
HpyAV CCTTC 2 cut(s) 140, 507
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 3 cut(s) 39, 329, 518
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 326, 335, 362
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 459
HspAI GCGC 1 cut(s) 409
Kzo9I GATC 1 cut(s) 511
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 3 cut(s) 179, 325, 527
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 32
MalI GATC 1 cut(s) 513
MbiI CCGCTC 1 cut(s) 255
MboI GATC 1 cut(s) 511
MboII GAAGA 2 cut(s) 107, 440
MflI RGATCY 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 3 cut(s) 59, 182, 282
MluNI TGGCCA 1 cut(s) 248
MnlI CCTC 4 cut(s) 197, 202, 316, 520
Mox20I TGGCCA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 248
MseI TTAA 1 cut(s) 281
MslI CAYNNNNRTG 1 cut(s) 159
Msp20I TGGCCA 1 cut(s) 248
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 235, 326, 335, 362
NdeII GATC 1 cut(s) 511
NlaIV GGNNCC 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 2 cut(s) 257, 367
PshBI ATTAAT 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 513
PsrI GAACNNNNNNTAC 2 cut(s) 25, 57
PstI CTGCAG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 2 cut(s) 256, 366
Sau3AI GATC 1 cut(s) 511
SetI ASST 6 cut(s) 54, 231, 276, 298, 327, 435
SfaNI GCATC 3 cut(s) 179, 325, 527
SfcI CTRYAG 2 cut(s) 37, 341
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 3 cut(s) 59, 182, 282
SsiI CCGC 1 cut(s) 255
SspI AATATT 2 cut(s) 417, 482
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 140, 277
TasI AATT 3 cut(s) 59, 182, 282
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 3 cut(s) 108, 269, 375
TseI GCWGC 1 cut(s) 365
TspDTI ATGAA 2 cut(s) 20, 128
TspRI CASTG 3 cut(s) 108, 269, 375
VspI ATTAAT 1 cut(s) 281
XagI CCTNNNNNAGG 1 cut(s) 437
XapI RAATTY 2 cut(s) 59, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.