Rh6CG472300

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0017464
Physical Location & Seq
Forward (+)
64222955 .. 64228518
5564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG472300.1

Sequence Viewer

Length: 603 bp
ATGTCTGGCTACCTTCCTGAAAAAACCATTCGTACAAGGATTAATGGAACATCAAAAGCTCTAGCCATATCACACTACCTTGTTCCTGAAGATATCATTCGTGAAATTAGTCAGATTCAGCACAGTGAGCAAGGCATAGAGCGCTCTATGATCGATAAGGATAAGATCGTCTCCCATCACTTGTCAGCCAGGGATGGCCGATTCGTCGTCGTTTTGGAGAAACCGTGCTATATTCAGTTCGAGTACTTGGTGTACTACGAGACCCGCATCACCGGAAAGCTGAGCTACGGCGCGATCACGGATCTGAAGGGCATTCAGGTACAAAGATTGTTCTTTTGGTTCGACGTGGATAAGATCAGGGTCGATTTGCCGCCGTCCGATAGTATTTACTTCACCGTTGGGATCATGAAGAAAAGCTCGATGTCGATCAGTTCCAGAATGTTCATTCTTGCCGGGACGGGCTTTCGGAAACAAAGGGAGGTGGAATCAGAAAGGATAGAGAAGATGCCGGAGGAACATGCAGCCGTGAACCTGCTTTCTAACTGTGCTCGAGGCTCGACATGGCTCCAGCATGGTCACACATCGACAAAACACATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

200

Amino Acids

22.98

Weight (kDa)

8.8

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 51 - 139 4.1e-21 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 540
AccII CGCG 1 cut(s) 293
AciI CCGC 2 cut(s) 265, 371
AclWI GGATC 2 cut(s) 309, 410
AcoI YGGCCR 1 cut(s) 196
AcuI CTGAAG 2 cut(s) 108, 326
AfaI GTAC 4 cut(s) 34, 245, 254, 321
AfeI AGCGCT 1 cut(s) 143
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 4 cut(s) 59, 280, 285, 417
AluI AGCT 4 cut(s) 59, 280, 285, 417
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 2 cut(s) 175, 254
AlwI GGATC 2 cut(s) 309, 410
Ama87I CYCGRG 1 cut(s) 549
Aor51HI AGCGCT 1 cut(s) 143
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 521
AseI ATTAAT 1 cut(s) 42
AspLEI GCGC 2 cut(s) 144, 293
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 2 cut(s) 262, 385
AvaI CYCGRG 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 1 cut(s) 533
BccI CCATC 2 cut(s) 183, 188
BceAI ACGGC 3 cut(s) 304, 358, 509
BciT130I CCWGG 1 cut(s) 190
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 175, 254
BfaI CTAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 145
BfuAI ACCTGC 1 cut(s) 540
BisI GCNGC 2 cut(s) 371, 522
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 2 cut(s) 372, 523
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 2 cut(s) 190, 454
BmeT110I CYCGRG 1 cut(s) 549
BmgBI CACGTC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 190, 454
BmsI GCATC 2 cut(s) 276, 495
BpmI CTGGAG 1 cut(s) 551
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 454
Bsa29I ATCGAT 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 189
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bse8I GATNNNNATC 1 cut(s) 425
BseBI CCWGG 1 cut(s) 190
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 199
BseJI GATNNNNATC 1 cut(s) 425
BseMII CTCAG 1 cut(s) 272
BseXI GCAGC 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 1 cut(s) 198
BshVI ATCGAT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 3 cut(s) 273, 453, 509
BslFI GGGAC 1 cut(s) 469
BsmAI GTCTC 2 cut(s) 175, 254
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 469
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 2 cut(s) 265, 371
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 1 cut(s) 273
BspDI ATCGAT 1 cut(s) 153
BspFNI CGCG 1 cut(s) 293
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 2 cut(s) 309, 410
BspTNI GGTCTC 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 4 cut(s) 125, 225, 397, 545
BstDEI CTNAG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 2 cut(s) 144, 293
BstKTI GATC 7 cut(s) 153, 168, 297, 304, 357, 405, 429
BstMAI GTCTC 2 cut(s) 175, 254
BstMBI GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
BstMWI GCNNNNNNNGC 2 cut(s) 127, 141
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 188, 452
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 1 cut(s) 533
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 1 cut(s) 198
BsuTUI ATCGAT 1 cut(s) 153
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 540
CciI TCATGA 1 cut(s) 405
CfoI GCGC 2 cut(s) 144, 293
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 4 cut(s) 33, 244, 253, 320
CviAII CATG 4 cut(s) 406, 518, 561, 572
CviQI GTAC 4 cut(s) 33, 244, 253, 320
DdeI CTNAG 1 cut(s) 281
DpnI GATC 7 cut(s) 152, 167, 296, 303, 356, 404, 428
DpnII GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
EaeI YGGCCR 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 94
Eco47III AGCGCT 1 cut(s) 143
Eco57I CTGAAG 2 cut(s) 108, 326
Eco88I CYCGRG 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 188
EcoRV GATATC 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 4 cut(s) 409, 521, 564, 575
FaiI YATR 8 cut(s) 68, 137, 149, 231, 407, 519, 562, 573
FaqI GGGAC 1 cut(s) 469
FatI CATG 4 cut(s) 405, 517, 560, 571
FauI CCCGC 1 cut(s) 272
Fnu4HI GCNGC 2 cut(s) 371, 522
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 2 cut(s) 371, 522
FspBI CTAG 1 cut(s) 62
GlaI GCGC 2 cut(s) 143, 292
GluI GCNGC 2 cut(s) 371, 522
GsuI CTGGAG 1 cut(s) 551
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 1 cut(s) 198
HapII CCGG 3 cut(s) 273, 453, 509
HhaI GCGC 2 cut(s) 144, 293
Hin1II CATG 4 cut(s) 409, 521, 564, 575
Hin6I GCGC 2 cut(s) 142, 291
HinP1I GCGC 2 cut(s) 142, 291
HinfI GANTC 3 cut(s) 115, 201, 485
HpaII CCGG 3 cut(s) 273, 453, 509
HphI GGTGA 2 cut(s) 262, 385
Hpy166II GTNNAC 2 cut(s) 253, 529
Hpy188I TCNGA 5 cut(s) 114, 306, 379, 468, 490
Hpy188III TCNNGA 5 cut(s) 17, 86, 101, 406, 435
Hpy8I GTNNAC 2 cut(s) 253, 529
Hpy99I CGWCG 3 cut(s) 209, 212, 347
HpyAV CCTTC 2 cut(s) 23, 301
HpyCH4III ACNGT 4 cut(s) 125, 225, 397, 545
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 1 cut(s) 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 141
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 4 cut(s) 409, 521, 564, 575
HspAI GCGC 2 cut(s) 142, 291
Kzo9I GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
LmnI GCTCC 1 cut(s) 570
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 2 cut(s) 276, 495
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 575
MalI GATC 7 cut(s) 152, 167, 296, 303, 356, 404, 428
MboI GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
MboII GAAGA 3 cut(s) 101, 421, 514
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 1 cut(s) 105
MnlI CCTC 3 cut(s) 472, 505, 545
MseI TTAA 1 cut(s) 42
MspI CCGG 3 cut(s) 273, 453, 509
MspR9I CCNGG 2 cut(s) 190, 454
Mva1269I GAATGC 1 cut(s) 312
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 127, 141
NciI CCSGG 1 cut(s) 454
NdeII GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
NlaIII CATG 4 cut(s) 409, 521, 564, 575
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 575
NspI RCATGY 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 549
PagI TCATGA 1 cut(s) 405
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 3 cut(s) 115, 201, 485
PkrI GCNGC 2 cut(s) 372, 523
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 566
PspXI VCTCGAGB 1 cut(s) 549
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 4 cut(s) 34, 245, 254, 321
RsaNI GTAC 4 cut(s) 33, 244, 253, 320
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 2 cut(s) 371, 522
Sau3AI GATC 7 cut(s) 150, 165, 294, 301, 354, 402, 426
ScaI AGTACT 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 190, 454
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 2 cut(s) 276, 495
Sfr274I CTCGAG 1 cut(s) 549
SlaI CTCGAG 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
Sse9I AATT 1 cut(s) 105
SsiI CCGC 2 cut(s) 265, 371
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 188, 452
TaaI ACNGT 4 cut(s) 125, 225, 397, 545
TaiI ACGT 1 cut(s) 348
TasI AATT 1 cut(s) 105
TatI WGTACW 2 cut(s) 243, 252
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 3 cut(s) 115, 201, 485
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 2 cut(s) 422, 433
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 1 cut(s) 130
VspI ATTAAT 1 cut(s) 42
XceI RCATGY 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 549
XspI CTAG 1 cut(s) 62
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.