Rorug06G0216600

Protein TIME FOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008448
Physical Location & Seq
Forward (+)
34615356 .. 34616750
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0216600.1

Sequence Viewer

Length: 900 bp
ATGTTCACAGCTCCAGTTCTCCAACAGATGCTGCACTCTCAATCCGGACAATTGGTCATGAACCAGAAGCCGTCGTGGAAGCCCCACGTCGAGATCGCTCCCAATTGCCCCCGCTGCGCGTCTGCCAACACCAAGTTCTGCTACTACAACAACTACAGCCTCTCGCAGCCGCGCTACTTCTGCAAAGGCTGCCGGAGATACTGGACCAAAGGCGGGTCCATGAGAAATGTGCCCGTCGGCGGCGGTTGCAGAAAGAACCGGCGCGGCAGGTCGGTCAGGCTCTCGCAGGCTGACCGGGTTTCGTTGAGTTACTATCGTCACAACAGCTCGAGTAGTAGCGGTGGTGATCTATCCGAGCAGCAGTCCACGGAAGGTGGAGCCAACGGATCGGATATCGACTTGGCTGCTGTGTTTGCTAAGTTCTTGAACCAAGACTCTAGCAACGAACATGATCCCAATTTGGTTGGATCAGAATCGATCCCCGATGATGTTCATGAGGCAAACGCATTATCTGATCACTCCCAAATTTCTTCGAACCACGCGGAAGATCTTCTTGAATCAGTGGAGCTACTTGAAGGACTCGTACTTCCTGATCAGGATCATCAACAATTACACGAAGGGGATAATATTCAGAATATTGTGGGAAATCAACACCATCATGATTTGAGTATTCATGAATTTGGGTTGCAGGGCTTGATAGGCGATGAGGATGTGTTTTGGACTGATACAACTGCAAGTTTGACAAGTAGTACTACTAGTTTCACATGGCAAGAGTTGGATAATTCATTCCCATCTAATGATGATGATCATATGAAGATATCGACCACCACGACGAATCTATGTAGCGATAACTGGAGCTCCTTTGATTTTTCAGGCTTGGAAGTTTTCTCAGGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

33.1

Weight (kDa)

5.02

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 34 - 88 9.1e-32 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 258
AccII CGCG 4 cut(s) 119, 172, 264, 542
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 8 cut(s) 112, 170, 213, 240, 243, 264, 339, 542
AclWI GGATC 5 cut(s) 394, 446, 472, 475, 606
AcsI RAATTY 2 cut(s) 525, 677
AfaI GTAC 2 cut(s) 585, 751
AfiI CCNNNNNNNGG 2 cut(s) 213, 239
AgsI TTSAA 3 cut(s) 427, 557, 575
AhdI GACNNNNNGTC 1 cut(s) 53
AhlI ACTAGT 1 cut(s) 755
AjiI CACGTC 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 4 cut(s) 11, 327, 568, 858
AluI AGCT 4 cut(s) 11, 327, 568, 858
Alw21I GWGCWC 1 cut(s) 860
AlwI GGATC 5 cut(s) 394, 446, 472, 475, 606
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 328
Aor13HI TCCGGA 1 cut(s) 44
ApeKI GCWGC 6 cut(s) 31, 114, 166, 189, 358, 404
ApoI RAATTY 2 cut(s) 525, 677
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 3 cut(s) 119, 174, 264
AspS9I GGNCC 2 cut(s) 204, 216
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 356
AsuII TTCGAA 1 cut(s) 533
AvaI CYCGRG 1 cut(s) 328
AvaII GGWCC 2 cut(s) 204, 216
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 860
BarI GAAGNNNNNNTAC 2 cut(s) 567, 599
Bbv12I GWGCWC 1 cut(s) 860
BbvI GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BccI CCATC 2 cut(s) 663, 799
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BclI TGATCA 3 cut(s) 514, 592, 805
BcnI CCSGG 1 cut(s) 296
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 2 cut(s) 438, 756
BfmI CTRYAG 1 cut(s) 154
BfuAI ACCTGC 1 cut(s) 258
BglII AGATCT 1 cut(s) 547
BisI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
BlsI GCNGC 9 cut(s) 33, 116, 168, 171, 191, 242, 266, 360, 406
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 204, 216
BmeRI GACNNNNNGTC 1 cut(s) 53
BmeT110I CYCGRG 1 cut(s) 328
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 204, 216
BmiI GGNNCC 2 cut(s) 217, 379
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 874
Bpu14I TTCGAA 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 476
BsaBI GATNNNNATC 3 cut(s) 472, 597, 804
BsaJI CCNNGG 1 cut(s) 366
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 239
Bse118I RCCGGY 1 cut(s) 258
Bse1I ACTGG 3 cut(s) 14, 206, 857
Bse8I GATNNNNATC 3 cut(s) 472, 597, 804
BseAI TCCGGA 1 cut(s) 44
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 3 cut(s) 472, 597, 804
BseLI CCNNNNNNNGG 2 cut(s) 213, 239
BseNI ACTGG 3 cut(s) 14, 206, 857
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BsgI GTGCAG 1 cut(s) 17
Bsh1236I CGCG 4 cut(s) 119, 172, 264, 542
BshVI ATCGAT 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 860
BsiHKCI CYCGRG 1 cut(s) 328
BsiSI CCGG 4 cut(s) 45, 193, 259, 295
BslI CCNNNNNNNGG 2 cut(s) 213, 239
BsoBI CYCGRG 1 cut(s) 328
Bsp119I TTCGAA 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 234, 860
Bsp13I TCCGGA 1 cut(s) 44
BspACI CCGC 8 cut(s) 112, 170, 213, 240, 243, 264, 339, 542
BspDI ATCGAT 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 44
BspFNI CGCG 4 cut(s) 119, 172, 264, 542
BspHI TCATGA 4 cut(s) 57, 493, 658, 673
BspLI GGNNCC 2 cut(s) 217, 379
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 5 cut(s) 394, 446, 472, 475, 606
BspT104I TTCGAA 1 cut(s) 533
BsrFI RCCGGY 1 cut(s) 258
BsrI ACTGG 3 cut(s) 14, 206, 857
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 417, 889
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 4 cut(s) 119, 172, 264, 542
BstHHI GCGC 3 cut(s) 119, 174, 264
BstMWI GCNNNNNNNGC 6 cut(s) 114, 180, 189, 246, 413, 699
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 4 cut(s) 119, 172, 264, 542
BstV1I GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 476
BsuTUI ATCGAT 1 cut(s) 476
BtgI CCRYGG 1 cut(s) 366
BtgZI GCGATG 1 cut(s) 717
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 288
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 4 cut(s) 57, 493, 658, 673
CfoI GCGC 3 cut(s) 119, 174, 264
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 204, 216
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 1 cut(s) 108
Csp6I GTAC 2 cut(s) 584, 750
CviAII CATG 7 cut(s) 58, 220, 449, 494, 659, 674, 765
CviQI GTAC 2 cut(s) 584, 750
DdeI CTNAG 2 cut(s) 417, 889
DriI GACNNNNNGTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 860
Eco32I GATATC 2 cut(s) 394, 819
Eco47I GGWCC 2 cut(s) 204, 216
Eco53kI GAGCTC 1 cut(s) 858
Eco88I CYCGRG 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 858
EcoRV GATATC 2 cut(s) 394, 819
EcoT38I GRGCYC 1 cut(s) 860
FaeI CATG 7 cut(s) 61, 223, 452, 497, 662, 677, 768
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FatI CATG 7 cut(s) 57, 219, 448, 493, 658, 673, 764
FauI CCCGC 2 cut(s) 119, 206
FauNDI CATATG 1 cut(s) 810
FbaI TGATCA 3 cut(s) 514, 592, 805
Fnu4HI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
FokI GGATG 1 cut(s) 722
FriOI GRGCYC 1 cut(s) 860
Fsp4HI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
FspBI CTAG 2 cut(s) 438, 756
GlaI GCGC 3 cut(s) 118, 173, 263
GluI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
GsuI CTGGAG 1 cut(s) 874
HapII CCGG 4 cut(s) 45, 193, 259, 295
HgaI GACGC 1 cut(s) 108
HhaI GCGC 3 cut(s) 119, 174, 264
Hin1II CATG 7 cut(s) 61, 223, 452, 497, 662, 677, 768
Hin6I GCGC 3 cut(s) 117, 172, 262
HinP1I GCGC 3 cut(s) 117, 172, 262
HinfI GANTC 5 cut(s) 434, 473, 557, 579, 835
HpaII CCGG 4 cut(s) 45, 193, 259, 295
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 2 cut(s) 6, 366
Hpy188I TCNGA 5 cut(s) 355, 391, 472, 514, 633
Hpy8I GTNNAC 2 cut(s) 6, 366
Hpy99I CGWCG 4 cut(s) 76, 92, 239, 835
HpyAV CCTTC 3 cut(s) 365, 569, 611
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 5 cut(s) 34, 183, 249, 688, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 180, 189, 246, 413, 699
HpyF3I CTNAG 2 cut(s) 417, 889
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 7 cut(s) 61, 223, 452, 497, 662, 677, 768
HspAI GCGC 3 cut(s) 117, 172, 262
Kpn2I TCCGGA 1 cut(s) 44
Ksp22I TGATCA 3 cut(s) 514, 592, 805
LmnI GCTCC 6 cut(s) 16, 103, 377, 565, 855, 863
Lsp1109I GCAGC 6 cut(s) 18, 101, 176, 178, 370, 391
LweI GCATC 1 cut(s) 18
MaeI CTAG 2 cut(s) 438, 756
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 2 cut(s) 308, 317
MboII GAAGA 4 cut(s) 522, 542, 557, 826
MfeI CAATTG 2 cut(s) 50, 103
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 2 cut(s) 234, 860
MluCI AATT 7 cut(s) 50, 103, 457, 525, 608, 677, 781
MlyI GAGTC 2 cut(s) 428, 573
MmeI TCCRAC 3 cut(s) 46, 445, 756
MnlI CCTC 3 cut(s) 170, 490, 700
MroI TCCGGA 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 4 cut(s) 45, 193, 259, 295
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 2 cut(s) 50, 103
MvnI CGCG 4 cut(s) 119, 172, 264, 542
MwoI GCNNNNNNNGC 6 cut(s) 114, 180, 189, 246, 413, 699
NciI CCSGG 1 cut(s) 296
NdeI CATATG 1 cut(s) 810
NlaIII CATG 7 cut(s) 61, 223, 452, 497, 662, 677, 768
NlaIV GGNNCC 2 cut(s) 217, 379
NmuCI GTSAC 1 cut(s) 317
NspV TTCGAA 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 328
PagI TCATGA 4 cut(s) 57, 493, 658, 673
PcsI WCGNNNNNNNCGW 1 cut(s) 827
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 3 cut(s) 473, 557, 835
PkrI GCNGC 9 cut(s) 33, 116, 168, 171, 191, 242, 266, 360, 406
PleI GAGTC 2 cut(s) 428, 573
PpsI GAGTC 2 cut(s) 428, 573
Psp124BI GAGCTC 1 cut(s) 860
PspN4I GGNNCC 2 cut(s) 217, 379
PspPI GGNCC 2 cut(s) 204, 216
PspXI VCTCGAGB 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 2 cut(s) 585, 751
RsaNI GTAC 2 cut(s) 584, 750
RseI CAYNNNNRTG 1 cut(s) 657
SacI GAGCTC 1 cut(s) 860
SatI GCNGC 9 cut(s) 32, 115, 167, 170, 190, 241, 265, 359, 405
Sau96I GGNCC 2 cut(s) 204, 216
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 2 cut(s) 428, 573
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 234, 860
SetI ASST 7 cut(s) 13, 90, 272, 329, 376, 570, 860
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 328
SfuI TTCGAA 1 cut(s) 533
SinI GGWCC 2 cut(s) 204, 216
SlaI CTCGAG 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 7 cut(s) 50, 103, 457, 525, 608, 677, 781
SsiI CCGC 8 cut(s) 112, 170, 213, 240, 243, 264, 339, 542
SspI AATATT 2 cut(s) 628, 637
SspMI CTAG 2 cut(s) 438, 756
SstI GAGCTC 1 cut(s) 860
StyD4I CCNGG 1 cut(s) 294
TaiI ACGT 1 cut(s) 90
TaqI TCGA 6 cut(s) 90, 329, 396, 476, 533, 821
TaqII GACCGA 1 cut(s) 262
TasI AATT 7 cut(s) 50, 103, 457, 525, 608, 677, 781
TatI WGTACW 1 cut(s) 749
TauI GCSGC 3 cut(s) 172, 243, 267
TfiI GAWTC 3 cut(s) 473, 557, 835
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 6 cut(s) 31, 114, 166, 189, 358, 404
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 6 cut(s) 74, 482, 662, 690, 774, 827
TspGWI ACGGA 2 cut(s) 383, 399
TspRI CASTG 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 204, 216
XapI RAATTY 2 cut(s) 525, 677
XhoI CTCGAG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 2 cut(s) 438, 756
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.