Rorug06G0226600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012026
Physical Location & Seq
Forward (+)
36381347 .. 36388159
6813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0226600.1

Sequence Viewer

Length: 1584 bp
ATGGCTGGTAGTGCGGCATCGTGTGCTGAGAGGGCGACCAGTGATATGCTGATTGGTCCAGACTGGGCAATAAACATTGAGTTATGCGACATTATCAATATGGAGCCCGGGCAAGCAAAAGATGCCTTGAAGATACTAAAGAAGCGCCTGGGCAGTAAAAATCCAAAAATACAACTTCTGGCTCTTTTTGCACTGGAGACTTTGAGCAAAAACTGTGGCGAGAATATTTTTCACTATATCGTTGAGCGTGATATCTTGCATGATATGGTCAAAATAGTAAAGAAGAAGCCAGACTTGAATGTTAGGGAAAAAATACTTATTTTAATAGATACCTGGCAAGAAGCTTTTGGGGGCGCAAGGGGAAGATATCCTCAATACTATGCAGCATATAACGAACTGAGGTCTGCTGGAGTTGAATTTCCACCACGAGAAGAGAACAGTGTACCGCTTTTTACTCCACCACAGACCCAGCCAGTAGTTCCTCCTGTTACATCATATGATGATGCTGCTATTCAGGCTTCTCTGCAGTCTGATGCTTCTGATCTTAGCTTGCCAGAGATTCAAAATGCTCGAGGAATTGCTGATGTCTTGATGGAAATGCTTAATGCCTTGGATGCTAATAATCCCGAGGGTCTGAAGGAAGAAGTTATTGTTGACCTTGTTGATCAGTGCCGTTCGTACCAAAAGCGTGTCATGCTTCTTGTGAATAACACTGCAGATGAGTCCCTTCTGTTTCAGGGATTGGCACTGAACGATGATCTGCAGAAGGTACTTAGTCGGCATGATGACATGGTGAAAGGAACTACAACAGGAGTAAGAGGACCGGAATCTTCTGTGCCACTTGTGAATGTGAACCATGAGGATGATGAGTCGGAGGATGAATTTGCCCAACTAGCTCCCAGGTCATCGAGAGATAATTCACAGGGGCAGGGCCAGAGATCAGCCAACTCCAATACTGAACCAGTGCGAGCCAGCCCACTTCTTCCTCCTCCACCCTCTTCAAAAAGGCCAATAACCCTTGGTCCTGGTGTAATTGATTATCTCAGTGGTGAAGCCTACAAATCTGGAGGATCCCCTGCAACTTCAGAATCCACGTCCTTTGCAGTTCCCGCTCATTCGAGCCCCAATTCTAGTTCACGGTTGACTCCATTGTCTTCCTCAGCCCCTTCATCCCATTCCACAAACACGACTCCATCACCTTTGTTCACTGGAAAGCCTATTTACGATGAACCTGCTCCAAGAAGCAAATCCATTGATGGGCTGCCTCCAGCTCCTTGGGATGCACAGGCTCCTGGAGGTTCTGTGTCTCCTGGAGGTTCTCAATCTCCTGTCAGCCTTCCACCTCCACCATCAAGGTATAATCAGAGACAACAGTTTTTTGAGCAGCAAGCAGGTGGTGCTCCTCAAACAAGTAGTGGGTCTGGTTTGTCTTATGACAGTTTAGTGGGACAGACTCAGAACCTCTCTCTTAATTCATCTACCCCAACGAAACAAGTAAAACAGGAAGATGCCCTTTTCAAAGACCTCGTCGATTTTGCGAAAGCCAAGTCATCTTCGTCTGCAAACCCCAATAGATCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

527

Amino Acids

56.68

Weight (kDa)

5.06

Isoelectric Point (pI)

58.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VHS PF00790 10 - 134 5.2e-27 VHS domain
GAT PF03127 194 - 267 1.5e-19 GAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47950 AT2G47950 AT3G62990
fragaria_vesca FvH4_2g24610
prunus_persica Prupe.1G143800_v2.0.a1 Prupe.8G251300_v2.0.a1
pyrus_communis pycom13g15110 pycom16g14850
rosa_chinensis RchiOBHm_Chr6g0292431
rosa_laevigata RLG00000012025
rosa_multiflora Rmu_sc0012226.1_g000006 Rmu_sc0021303.1_g000002
rosa_roxburghii Rroxscaffold_7G00175070
rosa_rugosa Rorug06G0226600
rosa_samantha Rh6AG339900 Rh6BG347100 Rh6CG354000 Rh6DG339900
rosa_wichuraiana Rw6G029580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1382
AasI GACNNNNNNGTC 1 cut(s) 1150
Acc36I ACCTGC 2 cut(s) 1240, 1382
AccBSI CCGCTC 1 cut(s) 1112
AciI CCGC 3 cut(s) 14, 446, 1110
AclWI GGATC 2 cut(s) 1065, 1078
AcsI RAATTY 2 cut(s) 416, 881
AcuI CTGAAG 2 cut(s) 656, 1068
AfaI GTAC 3 cut(s) 444, 680, 771
AfiI CCNNNNNNNGG 1 cut(s) 1257
AgsI TTSAA 6 cut(s) 130, 298, 416, 563, 1002, 1519
AjiI CACGTC 1 cut(s) 1095
AjnI CCWGG 6 cut(s) 147, 332, 899, 1024, 1291, 1309
AjuI GAANNNNNNNTTGG 1 cut(s) 1562
AluBI AGCT 4 cut(s) 344, 549, 896, 1271
AluI AGCT 4 cut(s) 344, 549, 896, 1271
Alw21I GWGCWC 1 cut(s) 1402
Alw26I GTCTC 3 cut(s) 191, 1311, 1360
AlwI GGATC 2 cut(s) 1065, 1078
Ama87I CYCGRG 3 cut(s) 107, 570, 626
AoxI GGCC 2 cut(s) 931, 1007
ApeKI GCWGC 4 cut(s) 383, 506, 1261, 1384
ApoI RAATTY 2 cut(s) 416, 881
ArsI GACNNNNNNTTYG 4 cut(s) 1512, 1532, 1544, 1564
AspLEI GCGC 2 cut(s) 147, 356
AspS9I GGNCC 4 cut(s) 56, 821, 931, 1022
AsuC2I CCSGG 2 cut(s) 108, 109
AsuHPI GGTGA 3 cut(s) 805, 1061, 1188
AvaI CYCGRG 3 cut(s) 107, 570, 626
AvaII GGWCC 3 cut(s) 56, 821, 1022
BamHI GGATCC 1 cut(s) 1070
BanII GRGCYC 2 cut(s) 108, 1124
BauI CACGAG 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 1146
Bbv12I GWGCWC 1 cut(s) 1402
BbvCI CCTCAGC 1 cut(s) 1159
BbvI GCAGC 4 cut(s) 395, 493, 1248, 1396
BccI CCATC 4 cut(s) 586, 1201, 1250, 1357
BceAI ACGGC 1 cut(s) 657
BcgI CGANNNNNNTGC 2 cut(s) 1214, 1248
BciT130I CCWGG 6 cut(s) 149, 334, 901, 1026, 1293, 1311
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 2 cut(s) 108, 109
BcoDI GTCTC 3 cut(s) 191, 1311, 1360
BfaI CTAG 2 cut(s) 893, 1131
BfmI CTRYAG 3 cut(s) 524, 714, 761
BfoI RGCGCY 1 cut(s) 148
BfuAI ACCTGC 2 cut(s) 1240, 1382
BisI GCNGC 5 cut(s) 15, 384, 507, 1262, 1385
BlsI GCNGC 5 cut(s) 16, 385, 508, 1263, 1386
Bme1390I CCNGG 8 cut(s) 108, 109, 149, 334, 901, 1026, 1293, 1311
Bme18I GGWCC 3 cut(s) 56, 821, 1022
BmeT110I CYCGRG 3 cut(s) 107, 570, 626
BmgBI CACGTC 1 cut(s) 1095
BmgT120I GGNCC 4 cut(s) 56, 821, 931, 1022
BmiI GGNNCC 3 cut(s) 105, 1072, 1290
BmrFI CCNGG 8 cut(s) 108, 109, 149, 334, 901, 1026, 1293, 1311
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 7 cut(s) 26, 112, 493, 523, 604, 1270, 1498
BmuI ACTGGG 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 1146
BpmI CTGGAG 6 cut(s) 215, 429, 1086, 1251, 1314, 1332
Bpu10I CCTNAGC 1 cut(s) 1159
BpuMI CCSGG 2 cut(s) 108, 109
BsaJI CCNNGG 7 cut(s) 107, 148, 609, 627, 899, 1018, 1274
BsaWI WCCGGW 1 cut(s) 823
BsaXI ACNNNNNCTCC 2 cut(s) 1287, 1317
Bsc4I CCNNNNNNNGG 1 cut(s) 1257
Bse1I ACTGG 6 cut(s) 39, 68, 198, 473, 962, 1213
BseBI CCWGG 6 cut(s) 149, 334, 901, 1026, 1293, 1311
BseDI CCNNGG 7 cut(s) 107, 148, 609, 627, 899, 1018, 1274
BseGI GGATG 5 cut(s) 619, 868, 883, 1169, 1285
BseLI CCNNNNNNNGG 1 cut(s) 1257
BseMII CTCAG 5 cut(s) 18, 389, 1057, 1173, 1469
BseNI ACTGG 6 cut(s) 39, 68, 198, 473, 962, 1213
BseRI GAGGAG 2 cut(s) 978, 1392
BseXI GCAGC 4 cut(s) 395, 493, 1248, 1396
BseYI CCCAGC 1 cut(s) 468
BshFI GGCC 2 cut(s) 933, 1009
BsiHKAI GWGCWC 1 cut(s) 1402
BsiHKCI CYCGRG 3 cut(s) 107, 570, 626
BsiSI CCGG 2 cut(s) 108, 824
BslFI GGGAC 2 cut(s) 709, 1461
BslI CCNNNNNNNGG 1 cut(s) 1257
BsmAI GTCTC 3 cut(s) 191, 1311, 1360
BsmFI GGGAC 2 cut(s) 709, 1461
BsnI GGCC 2 cut(s) 933, 1009
BsoBI CYCGRG 3 cut(s) 107, 570, 626
Bsp1286I GDGCHC 3 cut(s) 108, 1124, 1402
Bsp143I GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
BspACI CCGC 3 cut(s) 14, 446, 1110
BspANI GGCC 2 cut(s) 933, 1009
BspCNI CTCAG 5 cut(s) 19, 390, 1056, 1172, 1468
BspLI GGNNCC 3 cut(s) 105, 1072, 1290
BspMAI CTGCAG 3 cut(s) 528, 718, 765
BspMI ACCTGC 2 cut(s) 1240, 1382
BspPI GGATC 2 cut(s) 1065, 1078
BsrBI CCGCTC 1 cut(s) 1112
BsrI ACTGG 6 cut(s) 39, 68, 198, 473, 962, 1213
BssECI CCNNGG 7 cut(s) 107, 148, 609, 627, 899, 1018, 1274
BssMI GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
BssSI CACGAG 1 cut(s) 426
BssT1I CCWWGG 3 cut(s) 609, 1018, 1274
Bst2BI CACGAG 1 cut(s) 426
Bst2UI CCWGG 6 cut(s) 149, 334, 901, 1026, 1293, 1311
Bst4CI ACNGT 5 cut(s) 215, 440, 1140, 1374, 1439
Bst6I CTCTTC 2 cut(s) 426, 1003
BstAPI GCANNNNNTGC 3 cut(s) 23, 122, 1397
BstC8I GCNNGC 5 cut(s) 114, 551, 969, 973, 1389
BstDEI CTNAG 7 cut(s) 27, 398, 545, 773, 1043, 1159, 1455
BstF5I GGATG 5 cut(s) 619, 868, 883, 1169, 1285
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 2 cut(s) 147, 356
BstKTI GATC 6 cut(s) 544, 667, 760, 941, 1073, 1577
BstMAI GTCTC 3 cut(s) 191, 1311, 1360
BstMBI GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
BstNI CCWGG 6 cut(s) 149, 334, 901, 1026, 1293, 1311
BstSCI CCNGG 8 cut(s) 106, 107, 147, 332, 899, 1024, 1291, 1309
BstSFI CTRYAG 3 cut(s) 524, 714, 761
BstV1I GCAGC 4 cut(s) 395, 493, 1248, 1396
BstV2I GAAGAC 1 cut(s) 1146
BstX2I RGATCY 1 cut(s) 1070
BstXI CCANNNNNNTGG 1 cut(s) 1275
BstYI RGATCY 1 cut(s) 1070
BsuRI GGCC 2 cut(s) 933, 1009
BtrI CACGTC 1 cut(s) 1095
BtsCI GGATG 5 cut(s) 619, 868, 883, 1169, 1285
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 9 cut(s) 46, 191, 445, 674, 711, 746, 969, 1051, 1206
BveI ACCTGC 2 cut(s) 1240, 1382
Cac8I GCNNGC 5 cut(s) 114, 551, 969, 973, 1389
CfoI GCGC 2 cut(s) 147, 356
Cfr13I GGNCC 4 cut(s) 56, 821, 931, 1022
Cfr9I CCCGGG 1 cut(s) 107
Csp6I GTAC 3 cut(s) 443, 679, 770
CspCI CAANNNNNGTGG 4 cut(s) 196, 231, 1081, 1116
CviAII CATG 5 cut(s) 260, 694, 782, 790, 857
CviQI GTAC 3 cut(s) 443, 679, 770
DdeI CTNAG 7 cut(s) 27, 398, 545, 773, 1043, 1159, 1455
DpnI GATC 6 cut(s) 543, 666, 759, 940, 1072, 1576
DpnII GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
DrdI GACNNNNNNGTC 1 cut(s) 1150
DseDI GACNNNNNNGTC 1 cut(s) 1150
Eam1104I CTCTTC 2 cut(s) 426, 1003
EarI CTCTTC 2 cut(s) 426, 1003
Eco130I CCWWGG 3 cut(s) 609, 1018, 1274
Eco24I GRGCYC 2 cut(s) 108, 1124
Eco32I GATATC 2 cut(s) 253, 368
Eco47I GGWCC 3 cut(s) 56, 821, 1022
Eco57I CTGAAG 2 cut(s) 656, 1068
Eco88I CYCGRG 3 cut(s) 107, 570, 626
EcoRII CCWGG 6 cut(s) 147, 332, 899, 1024, 1291, 1309
EcoRV GATATC 2 cut(s) 253, 368
EcoT14I CCWWGG 3 cut(s) 609, 1018, 1274
EcoT38I GRGCYC 2 cut(s) 108, 1124
ErhI CCWWGG 3 cut(s) 609, 1018, 1274
FaeI CATG 5 cut(s) 263, 697, 785, 793, 860
FalI AAGNNNNNCTT 4 cut(s) 278, 310, 1497, 1529
FaqI GGGAC 2 cut(s) 709, 1461
FatI CATG 5 cut(s) 259, 693, 781, 789, 856
FauI CCCGC 1 cut(s) 1117
FauNDI CATATG 1 cut(s) 496
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 5 cut(s) 15, 384, 507, 1262, 1385
FokI GGATG 5 cut(s) 626, 875, 890, 1156, 1292
FriOI GRGCYC 2 cut(s) 108, 1124
Fsp4HI GCNGC 5 cut(s) 15, 384, 507, 1262, 1385
FspBI CTAG 2 cut(s) 893, 1131
GlaI GCGC 2 cut(s) 146, 355
GluI GCNGC 5 cut(s) 15, 384, 507, 1262, 1385
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 6 cut(s) 215, 429, 1086, 1251, 1314, 1332
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 2 cut(s) 933, 1009
HapII CCGG 2 cut(s) 108, 824
HhaI GCGC 2 cut(s) 147, 356
Hin1II CATG 5 cut(s) 263, 697, 785, 793, 860
Hin6I GCGC 2 cut(s) 145, 354
HinP1I GCGC 2 cut(s) 145, 354
HincII GTYRAC 2 cut(s) 655, 1143
HindII GTYRAC 2 cut(s) 655, 1143
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 8 cut(s) 559, 722, 827, 869, 1088, 1144, 1189, 1453
HpaII CCGG 2 cut(s) 108, 824
HphI GGTGA 3 cut(s) 805, 1061, 1188
Hpy166II GTNNAC 6 cut(s) 443, 655, 853, 1136, 1143, 1206
Hpy188I TCNGA 8 cut(s) 532, 541, 636, 874, 1087, 1365, 1458, 1583
Hpy188III TCNNGA 5 cut(s) 59, 589, 626, 909, 1065
Hpy8I GTNNAC 6 cut(s) 443, 655, 853, 1136, 1143, 1206
Hpy99I CGWCG 1 cut(s) 1532
HpyAV CCTTC 5 cut(s) 631, 737, 760, 1176, 1346
HpyCH4III ACNGT 5 cut(s) 215, 440, 1140, 1374, 1439
HpyCH4IV ACGT 1 cut(s) 1094
HpyF3I CTNAG 7 cut(s) 27, 398, 545, 773, 1043, 1159, 1455
HpySE526I ACGT 1 cut(s) 1094
Hsp92II CATG 5 cut(s) 263, 697, 785, 793, 860
HspAI GCGC 2 cut(s) 145, 354
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
LmnI GCTCC 6 cut(s) 103, 901, 1240, 1276, 1294, 1405
Lsp1109I GCAGC 4 cut(s) 395, 493, 1248, 1396
LweI GCATC 7 cut(s) 26, 112, 493, 523, 604, 1270, 1498
MaeI CTAG 2 cut(s) 893, 1131
MaeII ACGT 1 cut(s) 1094
MaeIII GTNAC 1 cut(s) 487
MalI GATC 6 cut(s) 543, 666, 759, 940, 1072, 1576
MbiI CCGCTC 1 cut(s) 1112
MboI GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
MflI RGATCY 1 cut(s) 1070
MhlI GDGCHC 3 cut(s) 108, 1124, 1402
MluCI AATT 7 cut(s) 416, 576, 881, 916, 1032, 1126, 1471
MlyI GAGTC 5 cut(s) 731, 878, 1138, 1183, 1447
MmeI TCCRAC 1 cut(s) 852
MseI TTAA 3 cut(s) 323, 603, 1470
MslI CAYNNNNRTG 1 cut(s) 861
MspI CCGG 2 cut(s) 108, 824
MspR9I CCNGG 8 cut(s) 108, 109, 149, 334, 901, 1026, 1293, 1311
MvaI CCWGG 6 cut(s) 149, 334, 901, 1026, 1293, 1311
NciI CCSGG 2 cut(s) 108, 109
NdeI CATATG 1 cut(s) 496
NdeII GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
NlaIII CATG 5 cut(s) 263, 697, 785, 793, 860
NlaIV GGNNCC 3 cut(s) 105, 1072, 1290
PaeR7I CTCGAG 1 cut(s) 570
PaqCI CACCTGC 1 cut(s) 1382
PfeI GAWTC 3 cut(s) 559, 827, 1088
PflFI GACNNNGTC 1 cut(s) 1526
PfoI TCCNGGA 2 cut(s) 1291, 1309
PkrI GCNGC 5 cut(s) 16, 385, 508, 1263, 1386
PleI GAGTC 5 cut(s) 730, 877, 1138, 1183, 1447
PpsI GAGTC 5 cut(s) 730, 877, 1138, 1183, 1447
Psp6I CCWGG 6 cut(s) 147, 332, 899, 1024, 1291, 1309
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 6 cut(s) 147, 332, 899, 1024, 1291, 1309
PspN4I GGNNCC 3 cut(s) 105, 1072, 1290
PspPI GGNCC 4 cut(s) 56, 821, 931, 1022
PspXI VCTCGAGB 1 cut(s) 570
PstI CTGCAG 3 cut(s) 528, 718, 765
PsuI RGATCY 1 cut(s) 1070
PsyI GACNNNGTC 1 cut(s) 1526
RsaI GTAC 3 cut(s) 444, 680, 771
RsaNI GTAC 3 cut(s) 443, 679, 770
RseI CAYNNNNRTG 1 cut(s) 861
SaqAI TTAA 3 cut(s) 323, 603, 1470
SatI GCNGC 5 cut(s) 15, 384, 507, 1262, 1385
Sau3AI GATC 6 cut(s) 541, 664, 757, 938, 1070, 1574
Sau96I GGNCC 4 cut(s) 56, 821, 931, 1022
SchI GAGTC 5 cut(s) 731, 878, 1138, 1183, 1447
ScrFI CCNGG 8 cut(s) 108, 109, 149, 334, 901, 1026, 1293, 1311
SduI GDGCHC 3 cut(s) 108, 1124, 1402
SfaNI GCATC 7 cut(s) 26, 112, 493, 523, 604, 1270, 1498
SfcI CTRYAG 3 cut(s) 524, 714, 761
Sfr274I CTCGAG 1 cut(s) 570
SinI GGWCC 3 cut(s) 56, 821, 1022
SlaI CTCGAG 1 cut(s) 570
SmaI CCCGGG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SrfI GCCCGGGC 1 cut(s) 109
Sse9I AATT 7 cut(s) 416, 576, 881, 916, 1032, 1126, 1471
SsiI CCGC 3 cut(s) 14, 446, 1110
SspI AATATT 1 cut(s) 226
SspMI CTAG 2 cut(s) 893, 1131
StyD4I CCNGG 8 cut(s) 106, 107, 147, 332, 899, 1024, 1291, 1309
StyI CCWWGG 3 cut(s) 609, 1018, 1274
TaaI ACNGT 5 cut(s) 215, 440, 1140, 1374, 1439
TaiI ACGT 1 cut(s) 1097
TaqI TCGA 4 cut(s) 571, 908, 1118, 1530
TasI AATT 7 cut(s) 416, 576, 881, 916, 1032, 1126, 1471
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 3 cut(s) 559, 827, 1088
Tru1I TTAA 3 cut(s) 323, 603, 1470
Tru9I TTAA 3 cut(s) 323, 603, 1470
TscAI CASTG 9 cut(s) 46, 198, 445, 674, 718, 753, 969, 1051, 1213
TseI GCWGC 4 cut(s) 383, 506, 1261, 1384
TspDTI ATGAA 4 cut(s) 894, 1158, 1242, 1464
TspMI CCCGGG 1 cut(s) 107
TspRI CASTG 9 cut(s) 46, 198, 445, 674, 718, 753, 969, 1051, 1213
Tth111I GACNNNGTC 1 cut(s) 1526
VpaK11BI GGWCC 3 cut(s) 56, 821, 1022
XapI RAATTY 2 cut(s) 416, 881
XhoI CTCGAG 1 cut(s) 570
XmaI CCCGGG 1 cut(s) 107
XspI CTAG 2 cut(s) 893, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.