Rorug06G0302900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013191
Physical Location & Seq
Reverse (-)
45425149 .. 45427478
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0302900.1

Sequence Viewer

Length: 672 bp
ATGGAAGCCACTCTGTTCACCTCGACGCCCCCATCCCCGGCCTTATCCCTAACCCGACCCGCCAGCCCATTCCTCTCCCTCCCCTCCCCTCCCCACCGCCGCCGCCTCCGCCTCCCCACCATCCGCTCCGCCATCTCCCGCACCAAGAAAGAAGAGACCGTCGAAACCATCAAGACCAATCTCGACAACTGCTACCTCATGGCCGGTATCAGCTACAAAGGCCTCACCGTCAAGCAATTCCAGGACCTCCGCAAAGCCCTACCTGACTCCACCAAGCTCATCGTCGCCAAGAACACTCTCGTCTACAAAGCCATCGAAGGAACCCCCTGGGAGGCTCTCAAGCCTTGCATGACCGGGATGAACGCCTGGCTGTTCGTCCACAGCGAGGAGATCCCGCCGGCGATTAAGCCCTACCGGGATTTCCAGAAGGAACGGAAGCTCGAGAACAATGACTTCACCGGAGCGGTGTTCGAGGGCAAGTTTTACGGCCCCGGAGACTTCAAGGCGCTGGAGACAATGCCGACGAGGCTGGAGATTTATGCCAAGTTGCTTGGCTCCCTCAAGGGGCCGGCGTCCAACCTCGTCGGGACGATTCAGGCGCCGGCGAGGGAGCTGGTCATGACTTTGCAGGCGTATGTGCAGAAATTGGAGGAGAGCGGCGGCGGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

24.79

Weight (kDa)

9.45

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L10 PF00466 47 - 141 1e-19 Ribosomal protein L10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19530 AT1G19530
fragaria_vesca FvH4_2g39630
malus_domestica MD08G1029700.v1.1
prunus_persica Prupe.1G377400_v2.0.a1 Prupe.2G019400_v2.0.a1 Prupe.2G019400_v2.0.a1 Prupe.2G019400_v2.0.a1
pyrus_communis pycom08g02360
rosa_chinensis RchiOBHm_Chr6g0301271
rosa_laevigata RLG00000011265
rosa_multiflora Rmu_sc0013354.1_g000014
rosa_roxburghii Rroxscaffold_7G00166970
rosa_rugosa Rorug06G0302900
rosa_samantha Rh6AG416500 Rh6DG416200 Rh6DG416300
rosa_wichuraiana Rw6G036050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 598
AccBSI CCGCTC 3 cut(s) 126, 464, 657
AccI GTMKAC 1 cut(s) 303
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 1 cut(s) 201
AcyI GRCGYC 3 cut(s) 26, 572, 599
AfiI CCNNNNNNNGG 4 cut(s) 37, 331, 385, 564
AgsI TTSAA 1 cut(s) 502
AjnI CCWGG 3 cut(s) 240, 326, 365
AluBI AGCT 4 cut(s) 213, 277, 439, 613
AluI AGCT 4 cut(s) 213, 277, 439, 613
Alw26I GTCTC 3 cut(s) 149, 489, 506
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 5 cut(s) 39, 201, 220, 487, 566
AspLEI GCGC 2 cut(s) 508, 601
AspS9I GGNCC 3 cut(s) 244, 488, 566
AsuC2I CCSGG 4 cut(s) 38, 355, 416, 492
AsuHPI GGTGA 3 cut(s) 10, 217, 448
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 598
BccI CCATC 5 cut(s) 40, 128, 140, 176, 320
BceAI ACGGC 1 cut(s) 502
BciT130I CCWGG 3 cut(s) 242, 328, 367
BcnI CCSGG 4 cut(s) 38, 355, 416, 492
BcoDI GTCTC 3 cut(s) 149, 489, 506
BfoI RGCGCY 2 cut(s) 509, 602
BglI GCCNNNNNGGC 1 cut(s) 526
BisI GCNGC 4 cut(s) 100, 103, 658, 661
BlsI GCNGC 4 cut(s) 101, 104, 659, 662
Bme1390I CCNGG 7 cut(s) 38, 242, 328, 355, 367, 416, 492
Bme18I GGWCC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 440
BmgT120I GGNCC 3 cut(s) 244, 488, 566
BmiI GGNNCC 5 cut(s) 322, 490, 556, 567, 600
BmrFI CCNGG 7 cut(s) 38, 242, 328, 355, 367, 416, 492
BpmI CTGGAG 2 cut(s) 530, 551
BpuEI CTTGAG 2 cut(s) 323, 545
BpuMI CCSGG 4 cut(s) 38, 355, 416, 492
BsaHI GRCGYC 3 cut(s) 26, 572, 599
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 4 cut(s) 36, 326, 327, 490
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 331, 385, 564
Bse118I RCCGGY 4 cut(s) 203, 397, 568, 601
BseBI CCWGG 3 cut(s) 242, 328, 367
BseDI CCNNGG 4 cut(s) 36, 326, 327, 490
BseGI GGATG 3 cut(s) 32, 120, 363
BseLI CCNNNNNNNGG 4 cut(s) 37, 331, 385, 564
BseRI GAGGAG 2 cut(s) 401, 665
BsgI GTGCAG 1 cut(s) 659
BshFI GGCC 5 cut(s) 41, 203, 222, 489, 568
BshNI GGYRCC 1 cut(s) 598
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 9 cut(s) 38, 204, 354, 398, 415, 459, 492, 569, 602
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 4 cut(s) 37, 331, 385, 564
BsmAI GTCTC 3 cut(s) 149, 489, 506
BsmFI GGGAC 1 cut(s) 601
BsnI GGCC 5 cut(s) 41, 203, 222, 489, 568
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 440
Bsp143I GATC 1 cut(s) 390
BspANI GGCC 5 cut(s) 41, 203, 222, 489, 568
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 5 cut(s) 322, 490, 556, 567, 600
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 149
BsrBI CCGCTC 3 cut(s) 126, 464, 657
BsrFI RCCGGY 4 cut(s) 203, 397, 568, 601
BssAI RCCGGY 4 cut(s) 203, 397, 568, 601
BssECI CCNNGG 4 cut(s) 36, 326, 327, 490
BssMI GATC 1 cut(s) 390
BssNI GRCGYC 3 cut(s) 26, 572, 599
Bst2UI CCWGG 3 cut(s) 242, 328, 367
Bst4CI ACNGT 2 cut(s) 160, 229
Bst6I CTCTTC 1 cut(s) 147
BstACI GRCGYC 3 cut(s) 26, 572, 599
BstC8I GCNNGC 6 cut(s) 64, 399, 570, 603, 630, 665
BstF5I GGATG 3 cut(s) 32, 120, 363
BstH2I RGCGCY 2 cut(s) 509, 602
BstHHI GCGC 2 cut(s) 508, 601
BstKTI GATC 1 cut(s) 393
BstMAI GTCTC 3 cut(s) 149, 489, 506
BstMBI GATC 1 cut(s) 390
BstMWI GCNNNNNNNGC 3 cut(s) 108, 219, 526
BstNI CCWGG 3 cut(s) 242, 328, 367
BstSCI CCNGG 7 cut(s) 36, 240, 326, 353, 365, 414, 490
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BsuRI GGCC 5 cut(s) 41, 203, 222, 489, 568
BtsCI GGATG 3 cut(s) 32, 120, 363
Cac8I GCNNGC 6 cut(s) 64, 399, 570, 603, 630, 665
CciI TCATGA 1 cut(s) 618
CfoI GCGC 2 cut(s) 508, 601
Cfr10I RCCGGY 4 cut(s) 203, 397, 568, 601
Cfr13I GGNCC 3 cut(s) 244, 488, 566
CseI GACGC 2 cut(s) 34, 561
CviAII CATG 3 cut(s) 199, 349, 619
DinI GGCGCC 1 cut(s) 600
DpnI GATC 1 cut(s) 392
DpnII GATC 1 cut(s) 390
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
EciI GGCGGA 2 cut(s) 98, 118
Eco147I AGGCCT 1 cut(s) 222
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 244
EcoRII CCWGG 3 cut(s) 240, 326, 365
EgeI GGCGCC 1 cut(s) 600
EheI GGCGCC 1 cut(s) 600
FaeI CATG 3 cut(s) 202, 352, 622
FaiI YATR 5 cut(s) 200, 350, 540, 620, 636
FaqI GGGAC 1 cut(s) 601
FatI CATG 3 cut(s) 198, 348, 618
FauI CCCGC 4 cut(s) 67, 146, 402, 656
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 4 cut(s) 100, 103, 658, 661
FokI GGATG 3 cut(s) 19, 107, 370
Fsp4HI GCNGC 4 cut(s) 100, 103, 658, 661
GlaI GCGC 2 cut(s) 507, 600
GluI GCNGC 4 cut(s) 100, 103, 658, 661
GsuI CTGGAG 2 cut(s) 530, 551
HaeII RGCGCY 2 cut(s) 509, 602
HaeIII GGCC 5 cut(s) 41, 203, 222, 489, 568
HapII CCGG 9 cut(s) 38, 204, 354, 398, 415, 459, 492, 569, 602
HgaI GACGC 2 cut(s) 34, 561
HhaI GCGC 2 cut(s) 508, 601
Hin1I GRCGYC 3 cut(s) 26, 572, 599
Hin1II CATG 3 cut(s) 202, 352, 622
Hin6I GCGC 2 cut(s) 506, 599
HinP1I GCGC 2 cut(s) 506, 599
HinfI GANTC 2 cut(s) 266, 592
HpaII CCGG 9 cut(s) 38, 204, 354, 398, 415, 459, 492, 569, 602
HphI GGTGA 3 cut(s) 10, 217, 448
Hpy166II GTNNAC 3 cut(s) 18, 304, 379
Hpy188III TCNNGA 6 cut(s) 172, 182, 424, 442, 586, 619
Hpy8I GTNNAC 3 cut(s) 18, 304, 379
Hpy99I CGWCG 5 cut(s) 28, 164, 287, 526, 587
HpyAV CCTTC 2 cut(s) 311, 421
HpyCH4III ACNGT 2 cut(s) 160, 229
HpyCH4V TGCA 3 cut(s) 348, 628, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 219, 526
Hsp92I GRCGYC 3 cut(s) 26, 572, 599
Hsp92II CATG 3 cut(s) 202, 352, 622
HspAI GCGC 2 cut(s) 506, 599
KasI GGCGCC 1 cut(s) 598
KroI GCCGGC 3 cut(s) 397, 568, 601
KroNI GCCGGC 3 cut(s) 399, 570, 603
Kzo9I GATC 1 cut(s) 390
LmnI GCTCC 4 cut(s) 131, 461, 560, 610
MalI GATC 1 cut(s) 392
MbiI CCGCTC 3 cut(s) 126, 464, 657
MboI GATC 1 cut(s) 390
MboII GAAGA 1 cut(s) 164
MflI RGATCY 1 cut(s) 390
MluCI AATT 2 cut(s) 236, 644
Mly113I GGCGCC 1 cut(s) 599
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 600
MreI CGCCGGCG 2 cut(s) 397, 601
MroNI GCCGGC 3 cut(s) 397, 568, 601
MseI TTAA 1 cut(s) 405
MspI CCGG 9 cut(s) 38, 204, 354, 398, 415, 459, 492, 569, 602
MspR9I CCNGG 7 cut(s) 38, 242, 328, 355, 367, 416, 492
MvaI CCWGG 3 cut(s) 242, 328, 367
MwoI GCNNNNNNNGC 3 cut(s) 108, 219, 526
NaeI GCCGGC 3 cut(s) 399, 570, 603
NarI GGCGCC 1 cut(s) 599
NciI CCSGG 4 cut(s) 38, 355, 416, 492
NdeII GATC 1 cut(s) 390
NgoMIV GCCGGC 3 cut(s) 397, 568, 601
NlaIII CATG 3 cut(s) 202, 352, 622
NlaIV GGNNCC 5 cut(s) 322, 490, 556, 567, 600
PaeR7I CTCGAG 1 cut(s) 440
PagI TCATGA 1 cut(s) 618
PasI CCCWGGG 1 cut(s) 327
PceI AGGCCT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdiI GCCGGC 3 cut(s) 399, 570, 603
PfeI GAWTC 1 cut(s) 592
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 4 cut(s) 101, 104, 659, 662
PleI GAGTC 1 cut(s) 260
PluTI GGCGCC 1 cut(s) 602
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 3 cut(s) 240, 326, 365
PspGI CCWGG 3 cut(s) 240, 326, 365
PspN4I GGNNCC 5 cut(s) 322, 490, 556, 567, 600
PspPI GGNCC 3 cut(s) 244, 488, 566
PspPPI RGGWCCY 1 cut(s) 244
PsuI RGATCY 1 cut(s) 390
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 4 cut(s) 100, 103, 658, 661
Sau3AI GATC 1 cut(s) 390
Sau96I GGNCC 3 cut(s) 244, 488, 566
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 7 cut(s) 38, 242, 328, 355, 367, 416, 492
SetI ASST 9 cut(s) 23, 198, 215, 249, 265, 279, 441, 582, 615
SfoI GGCGCC 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 440
SgrAI CRCCGGYG 2 cut(s) 397, 601
SinI GGWCC 1 cut(s) 244
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 3 cut(s) 338, 440, 560
SmoI CTYRAG 3 cut(s) 338, 440, 560
Sse9I AATT 2 cut(s) 236, 644
SseBI AGGCCT 1 cut(s) 222
SspDI GGCGCC 1 cut(s) 598
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 7 cut(s) 36, 240, 326, 353, 365, 414, 490
TaaI ACNGT 2 cut(s) 160, 229
TaqI TCGA 6 cut(s) 23, 162, 183, 315, 441, 471
TasI AATT 2 cut(s) 236, 644
TauI GCSGC 4 cut(s) 102, 105, 660, 663
TfiI GAWTC 1 cut(s) 592
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TspDTI ATGAA 1 cut(s) 374
TspGWI ACGGA 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.