Rorug06G0405500

Rhodanese-like domain-containing protein 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008087
Physical Location & Seq
Forward (+)
54924739 .. 54935096
10358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0405500.1

Sequence Viewer

Length: 1080 bp
ATGAAAAAGAAGGGAAACTCGGATGGTGACCGCGTAGTGTTGGTGGAGTTGAAACAAATGTTTGGGGACTTAACTCTCAATGTGATTCTTAAAATGATTGCAGGGAAGCGATATTCTGTTGCGACCAATGAGGATGAGAAGAAAGAAGCGTGTCGTGTCCAGACTGCCTTGAGAGAGTTCTTCTATTTTGCAAGGCTGTTTGCGGTGGGTGATGCGATTCCTTATCTTCGGTGGTTGGATTTGGGTGGACATGAGAAGGCGATGAAGAAAACTGTAAAAGAAATGGACACCATTGTTGGGGGCTGGCTAGAAGAGCATAAGCGAAAAAGAGAACTCGGAGAAGAAGGAGATGATATAGAAGAGCCCGGTGACATCATGGACGCTTTGATTTTTGCCCTGGAAGGCGTAGACCTTGGTGGTTATGATGCTGATACTGTCAACAAAGCCACATGTTTGGATTTGATTTTAGGAGGTAGCGACACCACCATGGCTACATTGACATGGGCAGTATCACTATTATTGAACAATCGTCAAGTATTGAAACAAGCTCAAGATGAATTGGATGCAGAAGTCGGCAGAGAAAAAATTGTGAGCGAGTCGGATACAAGCAAGCTGGTGTATATCCAAGCCATAGTGAAAGAAACGCTGCGCTTATACCCTGCAGTACCATTATCAGCACAGCATGAGTTCACGGAGGACTGCACCGTGAATGGCTATCACGTCGAAAAGGGGACCCGCTTGATCACGAACCTTTGGAAGATTCAAACCAATCCACGAATCTGGCCTGACCCATTAGAATTCAAACCGGAGAGATTTCTCACCACTCACATGAACATGGATGTTAGGGGTCAGCATTTTGAACTAATCCCTTTCGGAAGTGGTAGAAGATCTTGTCCTGGGTTATCGTTTGCTCTTCAGATGGTACAATTTACTTTGGCTAGCTTTTTACATGCATTCGAAATCTCAACACCATCCAATGAGCTCGTTGATATGTCTGAGAGTTTTGGACTCACAAATGTTAAGGCCACCCCACTTAAAGTTTTCATCAAACCTCGCTTATGTACTAAACTTTATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese Rhodanese_C

Protein Analysis

359

Amino Acids

40.52

Weight (kDa)

5.46

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 14 - 336 1.8e-68 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 408
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 31, 203, 736
AcsI RAATTY 1 cut(s) 797
AcuI CTGAAG 1 cut(s) 899
AfaI GTAC 3 cut(s) 666, 924, 1063
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 6 cut(s) 52, 523, 541, 764, 802, 860
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 2 cut(s) 396, 895
AluBI AGCT 4 cut(s) 548, 613, 942, 982
AluI AGCT 4 cut(s) 548, 613, 942, 982
Alw21I GWGCWC 1 cut(s) 984
AoxI GGCC 2 cut(s) 782, 1023
ApeKI GCWGC 1 cut(s) 646
ApoI RAATTY 1 cut(s) 797
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 1 cut(s) 732
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 4 cut(s) 38, 221, 380, 811
AsuII TTCGAA 1 cut(s) 957
AsuNHI GCTAGC 1 cut(s) 938
AvaII GGWCC 1 cut(s) 732
BanII GRGCYC 2 cut(s) 366, 984
Bbv12I GWGCWC 1 cut(s) 984
BbvI GCAGC 1 cut(s) 633
BccI CCATC 3 cut(s) 17, 913, 979
BciT130I CCWGG 2 cut(s) 398, 897
BciVI GTATCC 1 cut(s) 595
BclI TGATCA 1 cut(s) 741
BcnI CCSGG 1 cut(s) 366
BfaI CTAG 2 cut(s) 308, 939
BfmI CTRYAG 1 cut(s) 660
BfuI GTATCC 1 cut(s) 595
BglII AGATCT 1 cut(s) 887
BisI GCNGC 1 cut(s) 647
BlsI GCNGC 1 cut(s) 648
Bme1390I CCNGG 3 cut(s) 366, 398, 897
Bme18I GGWCC 1 cut(s) 732
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 1 cut(s) 732
BmiI GGNNCC 2 cut(s) 733, 734
BmrFI CCNGG 3 cut(s) 366, 398, 897
BmsI GCATC 3 cut(s) 202, 415, 553
BmtI GCTAGC 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 957
BpuEI CTTGAG 2 cut(s) 190, 534
BpuMI CCSGG 1 cut(s) 366
BsaJI CCNNGG 4 cut(s) 396, 412, 486, 896
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 1 cut(s) 297
BseBI CCWGG 2 cut(s) 398, 897
BseDI CCNNGG 4 cut(s) 396, 412, 486, 896
BseGI GGATG 5 cut(s) 28, 139, 568, 844, 971
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 987
BseXI GCAGC 1 cut(s) 633
BsgI GTGCAG 1 cut(s) 685
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 784, 1025
BsiHKAI GWGCWC 1 cut(s) 984
BsiSI CCGG 2 cut(s) 366, 806
BslFI GGGAC 2 cut(s) 80, 745
BslI CCNNNNNNNGG 1 cut(s) 297
BsmFI GGGAC 2 cut(s) 80, 745
BsmI GAATGC 1 cut(s) 953
BsnI GGCC 2 cut(s) 784, 1025
Bsp119I TTCGAA 1 cut(s) 957
Bsp1286I GDGCHC 2 cut(s) 366, 984
Bsp143I GATC 2 cut(s) 741, 887
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 3 cut(s) 31, 203, 736
BspANI GGCC 2 cut(s) 784, 1025
BspCNI CTCAG 1 cut(s) 988
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 733, 734
BspMAI CTGCAG 1 cut(s) 664
BspOI GCTAGC 1 cut(s) 942
BspQI GCTCTTC 3 cut(s) 306, 354, 918
BspT104I TTCGAA 1 cut(s) 957
BssECI CCNNGG 4 cut(s) 396, 412, 486, 896
BssMI GATC 2 cut(s) 741, 887
BssT1I CCWWGG 2 cut(s) 412, 486
Bst2UI CCWGG 2 cut(s) 398, 897
Bst4CI ACNGT 3 cut(s) 274, 436, 706
Bst6I CTCTTC 3 cut(s) 306, 354, 918
BstBI TTCGAA 1 cut(s) 957
BstC8I GCNNGC 3 cut(s) 305, 611, 940
BstDEI CTNAG 1 cut(s) 996
BstDSI CCRYGG 1 cut(s) 486
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 5 cut(s) 28, 139, 568, 844, 971
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 2 cut(s) 744, 890
BstMBI GATC 2 cut(s) 741, 887
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstNI CCWGG 2 cut(s) 398, 897
BstNSI RCATGY 2 cut(s) 453, 953
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 3 cut(s) 364, 396, 895
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 887
BstXI CCANNNNNNTGG 2 cut(s) 454, 780
BstYI RGATCY 1 cut(s) 887
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 2 cut(s) 784, 1025
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 275
BtrI CACGTC 1 cut(s) 721
BtsCI GGATG 5 cut(s) 28, 139, 568, 844, 971
Cac8I GCNNGC 3 cut(s) 305, 611, 940
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 1 cut(s) 732
CseI GACGC 1 cut(s) 389
Csp6I GTAC 3 cut(s) 665, 923, 1062
CviAII CATG 9 cut(s) 251, 376, 450, 487, 501, 683, 829, 835, 950
CviQI GTAC 3 cut(s) 665, 923, 1062
DdeI CTNAG 1 cut(s) 996
DpnI GATC 2 cut(s) 743, 889
DpnII GATC 2 cut(s) 741, 887
Eam1104I CTCTTC 3 cut(s) 306, 354, 918
EarI CTCTTC 3 cut(s) 306, 354, 918
Ecl136II GAGCTC 1 cut(s) 982
Eco130I CCWWGG 2 cut(s) 412, 486
Eco24I GRGCYC 2 cut(s) 366, 984
Eco47I GGWCC 1 cut(s) 732
Eco53kI GAGCTC 1 cut(s) 982
Eco57I CTGAAG 1 cut(s) 899
Eco91I GGTNACC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 982
EcoO109I RGGNCCY 1 cut(s) 732
EcoO65I GGTNACC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 2 cut(s) 396, 895
EcoT14I CCWWGG 2 cut(s) 412, 486
EcoT22I ATGCAT 1 cut(s) 955
EcoT38I GRGCYC 2 cut(s) 366, 984
ErhI CCWWGG 2 cut(s) 412, 486
FaeI CATG 9 cut(s) 254, 379, 453, 490, 504, 686, 832, 838, 953
FaqI GGGAC 2 cut(s) 80, 745
FatI CATG 9 cut(s) 250, 375, 449, 486, 500, 682, 828, 834, 949
FauI CCCGC 1 cut(s) 743
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 1 cut(s) 647
FokI GGATG 5 cut(s) 35, 146, 575, 851, 958
FriOI GRGCYC 2 cut(s) 366, 984
Fsp4HI GCNGC 1 cut(s) 647
FspBI CTAG 2 cut(s) 308, 939
GlaI GCGC 1 cut(s) 650
GluI GCNGC 1 cut(s) 647
HaeIII GGCC 2 cut(s) 784, 1025
HapII CCGG 2 cut(s) 366, 806
HgaI GACGC 1 cut(s) 389
HhaI GCGC 1 cut(s) 651
Hin1II CATG 9 cut(s) 254, 379, 453, 490, 504, 686, 832, 838, 953
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 6 cut(s) 85, 217, 596, 760, 777, 1008
HpaII CCGG 2 cut(s) 366, 806
HphI GGTGA 4 cut(s) 38, 221, 380, 811
Hpy166II GTNNAC 4 cut(s) 248, 409, 439, 690
Hpy188I TCNGA 6 cut(s) 22, 338, 601, 875, 918, 997
Hpy188III TCNNGA 3 cut(s) 160, 551, 745
Hpy8I GTNNAC 4 cut(s) 248, 409, 439, 690
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 4 cut(s) 4, 250, 338, 395
HpyCH4III ACNGT 3 cut(s) 274, 436, 706
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 6 cut(s) 101, 191, 566, 662, 702, 953
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 1 cut(s) 996
HpySE526I ACGT 1 cut(s) 720
Hsp92II CATG 9 cut(s) 254, 379, 453, 490, 504, 686, 832, 838, 953
HspAI GCGC 1 cut(s) 649
KflI GGGWCCC 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 2 cut(s) 741, 887
LguI GCTCTTC 3 cut(s) 306, 354, 918
Lsp1109I GCAGC 1 cut(s) 633
LweI GCATC 3 cut(s) 202, 415, 553
MaeI CTAG 2 cut(s) 308, 939
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 2 cut(s) 26, 368
MalI GATC 2 cut(s) 743, 889
MboI GATC 2 cut(s) 741, 887
MflI RGATCY 1 cut(s) 887
MhlI GDGCHC 2 cut(s) 366, 984
MluCI AATT 4 cut(s) 557, 585, 797, 926
MlyI GAGTC 2 cut(s) 605, 1002
MmeI TCCRAC 2 cut(s) 216, 579
MnlI CCTC 4 cut(s) 124, 464, 688, 1062
Mph1103I ATGCAT 1 cut(s) 955
MseI TTAA 4 cut(s) 71, 90, 1020, 1035
MslI CAYNNNNRTG 4 cut(s) 485, 499, 827, 833
MspI CCGG 2 cut(s) 366, 806
MspR9I CCNGG 3 cut(s) 366, 398, 897
Mva1269I GAATGC 1 cut(s) 953
MvaI CCWGG 2 cut(s) 398, 897
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 313
NciI CCSGG 1 cut(s) 366
NcoI CCATGG 1 cut(s) 486
NdeII GATC 2 cut(s) 741, 887
NheI GCTAGC 1 cut(s) 938
NlaIII CATG 9 cut(s) 254, 379, 453, 490, 504, 686, 832, 838, 953
NlaIV GGNNCC 2 cut(s) 733, 734
NmuCI GTSAC 2 cut(s) 26, 368
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 2 cut(s) 453, 953
NspV TTCGAA 1 cut(s) 957
PciI ACATGT 1 cut(s) 449
PciSI GCTCTTC 3 cut(s) 306, 354, 918
PctI GAATGC 1 cut(s) 953
PfeI GAWTC 4 cut(s) 85, 217, 760, 777
PkrI GCNGC 1 cut(s) 648
PleI GAGTC 2 cut(s) 604, 1002
PpsI GAGTC 2 cut(s) 604, 1002
PpuMI RGGWCCY 1 cut(s) 732
PscI ACATGT 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 732
Psp6I CCWGG 2 cut(s) 396, 895
PspEI GGTNACC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 396, 895
PspN4I GGNNCC 2 cut(s) 733, 734
PspPI GGNCC 1 cut(s) 732
PspPPI RGGWCCY 1 cut(s) 732
PstI CTGCAG 1 cut(s) 664
PsuI RGATCY 1 cut(s) 887
RsaI GTAC 3 cut(s) 666, 924, 1063
RsaNI GTAC 3 cut(s) 665, 923, 1062
RseI CAYNNNNRTG 4 cut(s) 485, 499, 827, 833
SacI GAGCTC 1 cut(s) 984
SapI GCTCTTC 3 cut(s) 306, 354, 918
SaqAI TTAA 4 cut(s) 71, 90, 1020, 1035
SatI GCNGC 1 cut(s) 647
Sau3AI GATC 2 cut(s) 741, 887
Sau96I GGNCC 1 cut(s) 732
SchI GAGTC 2 cut(s) 605, 1002
ScrFI CCNGG 3 cut(s) 366, 398, 897
SduI GDGCHC 2 cut(s) 366, 984
SetI ASST 9 cut(s) 414, 475, 550, 615, 723, 753, 944, 984, 1054
SfaNI GCATC 3 cut(s) 202, 415, 553
SfcI CTRYAG 1 cut(s) 660
SfuI TTCGAA 1 cut(s) 957
SinI GGWCC 1 cut(s) 732
SmiMI CAYNNNNRTG 4 cut(s) 485, 499, 827, 833
SmlI CTYRAG 2 cut(s) 169, 549
SmoI CTYRAG 2 cut(s) 169, 549
Sse9I AATT 4 cut(s) 557, 585, 797, 926
SsiI CCGC 3 cut(s) 31, 203, 736
SspMI CTAG 2 cut(s) 308, 939
SstI GAGCTC 1 cut(s) 984
StyD4I CCNGG 3 cut(s) 364, 396, 895
StyI CCWWGG 2 cut(s) 412, 486
TaaI ACNGT 3 cut(s) 274, 436, 706
TaiI ACGT 1 cut(s) 723
TaqI TCGA 2 cut(s) 723, 957
TasI AATT 4 cut(s) 557, 585, 797, 926
TatI WGTACW 1 cut(s) 1061
TfiI GAWTC 4 cut(s) 85, 217, 760, 777
Tru1I TTAA 4 cut(s) 71, 90, 1020, 1035
Tru9I TTAA 4 cut(s) 71, 90, 1020, 1035
TseFI GTSAC 2 cut(s) 26, 368
TseI GCWGC 1 cut(s) 646
Tsp45I GTSAC 2 cut(s) 26, 368
TspDTI ATGAA 5 cut(s) 17, 278, 570, 845, 1033
TspGWI ACGGA 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 732
XapI RAATTY 1 cut(s) 797
XceI RCATGY 2 cut(s) 453, 953
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 2 cut(s) 308, 939
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.