Rh7DG004900

Rhodanese-like domain-containing protein 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008087
Physical Location & Seq
Forward (+)
437244 .. 438459
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG004900.1

Sequence Viewer

Length: 864 bp
ATGAGAGGGTGTTTCCCGGCGCCGGCTGTCATCTCCGGTGCGGCGAGCTCCGCAATCACTCAAATTGTTTGGGGAAGAAGAAAAGATCCGTTGCTTCAATTCTCAGTCTGGTCCTCAACAACTGCAACTTCGTCTTGCTTAGTCTATAATAGCAATAAGCATGCTGAGCGGAGACGAAGGCTGTGGCTTTCGTTATCAGAAGCAACAAGTAGAGGGAGAGGTGTGAGCGTTGGACTAGAGCAACAATGCGAGTGTGAAAGTGAAAGCAGAGAAGATAATGAATGGGTGGTGGTCAATTTTTACCAGTTTGTGTTGATCAAAGACGCGGAGGCACAAGTGGCCAAGCATCTCAATTTCTTGAAGGGGCGTGATATACGTGGGCGGATATATGTGAATGAACAAGGGATAAATGCGCAGTACAGTGGGCCGGCAAGAGACGCGCTTGCTTATGTTGAATGGGTGAGAGAGGATGAGAGATTTGCCAAATGCTTGGTTCAGATTTCTGCAGCACCAACTGGGCATGCCTTTCCAAAACTCAGGCTGCGGTATAAGCCTTCACTGGTTCAGTTGGAAGGAGGTATTAATCATCTTCCTTTGCTGGACCCTTCAATGCGAGCTACACCTCTAGCACCATCCGTGTGGAGGAAAAGATTAGAGACAGCCAACGGCAGCACTACTGCTGATGCATCAACTCAAAATCCTAGACCAAACTATATTCTATTGGATGTTAGAAACGGTTATGAGTGGGATATTGGTCATTTCAGTGGGGCTCGACGGCCAGATGTGGACTGCTTCAGGAGCACTTCGTTTGGGCTATGCCAACCAGAGGTCTGCTGCCTCAACACGCGTAGATCTAGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese Rhodanese_C

Protein Analysis

287

Amino Acids

32.26

Weight (kDa)

9.21

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 95 - 185 1.1e-16 UPF0176 acylphosphatase like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 19
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 3 cut(s) 326, 440, 847
AciI CCGC 6 cut(s) 41, 51, 169, 326, 382, 544
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 2 cut(s) 339, 776
AcuI CTGAAG 1 cut(s) 778
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 22, 642, 826
AflIII ACRYGT 1 cut(s) 845
AgsI TTSAA 4 cut(s) 98, 361, 455, 609
AluBI AGCT 2 cut(s) 48, 617
AluI AGCT 2 cut(s) 48, 617
Alw21I GWGCWC 2 cut(s) 50, 803
Alw26I GTCTC 3 cut(s) 166, 429, 650
AlwI GGATC 1 cut(s) 80
AoxI GGCC 3 cut(s) 339, 425, 776
ApeKI GCWGC 4 cut(s) 506, 541, 669, 834
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 3 cut(s) 22, 415, 442
AspS9I GGNCC 3 cut(s) 111, 425, 601
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 472
AvaII GGWCC 2 cut(s) 111, 601
BalI TGGCCA 1 cut(s) 341
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 2 cut(s) 50, 772
Bbv12I GWGCWC 2 cut(s) 50, 803
BbvI GCAGC 4 cut(s) 518, 528, 681, 821
BccI CCATC 1 cut(s) 640
BceAI ACGGC 2 cut(s) 682, 791
BclI TGATCA 1 cut(s) 315
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 3 cut(s) 166, 429, 650
BfaI CTAG 4 cut(s) 236, 626, 702, 855
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 23
BglII AGATCT 1 cut(s) 851
BisI GCNGC 5 cut(s) 42, 507, 542, 670, 835
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 5 cut(s) 43, 508, 543, 671, 836
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 2 cut(s) 111, 601
BmgT120I GGNCC 3 cut(s) 111, 425, 601
BmiI GGNNCC 2 cut(s) 21, 603
BmrFI CCNGG 1 cut(s) 17
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 3 cut(s) 355, 673, 695
BmuI ACTGGG 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 377
BsaHI GRCGYC 1 cut(s) 20
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 642, 826
Bse118I RCCGGY 2 cut(s) 22, 427
Bse1I ACTGG 3 cut(s) 304, 520, 564
BseGI GGATG 3 cut(s) 475, 632, 730
BseLI CCNNNNNNNGG 3 cut(s) 22, 642, 826
BseMII CTCAG 3 cut(s) 117, 156, 550
BseNI ACTGG 3 cut(s) 304, 520, 564
BseXI GCAGC 4 cut(s) 518, 528, 681, 821
Bsh1236I CGCG 3 cut(s) 326, 440, 847
BshFI GGCC 3 cut(s) 341, 427, 778
BshNI GGYRCC 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 50, 803
BsiSI CCGG 4 cut(s) 17, 23, 36, 428
BslI CCNNNNNNNGG 3 cut(s) 22, 642, 826
BsmAI GTCTC 3 cut(s) 166, 429, 650
BsmBI CGTCTC 2 cut(s) 166, 429
BsnI GGCC 3 cut(s) 341, 427, 778
Bsp1286I GDGCHC 3 cut(s) 50, 772, 803
Bsp143I GATC 3 cut(s) 85, 315, 851
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 6 cut(s) 41, 51, 169, 326, 382, 544
BspANI GGCC 3 cut(s) 341, 427, 778
BspCNI CTCAG 3 cut(s) 116, 157, 549
BspFNI CGCG 3 cut(s) 326, 440, 847
BspLI GGNNCC 2 cut(s) 21, 603
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 19
BsrBI CCGCTC 1 cut(s) 169
BsrFI RCCGGY 2 cut(s) 22, 427
BsrI ACTGG 3 cut(s) 304, 520, 564
BssAI RCCGGY 2 cut(s) 22, 427
BssMI GATC 3 cut(s) 85, 315, 851
BssNI GRCGYC 1 cut(s) 20
Bst4CI ACNGT 2 cut(s) 422, 737
BstACI GRCGYC 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 7 cut(s) 24, 46, 162, 429, 444, 522, 615
BstDEI CTNAG 4 cut(s) 103, 139, 165, 536
BstF5I GGATG 3 cut(s) 475, 632, 730
BstFNI CGCG 3 cut(s) 326, 440, 847
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 3 cut(s) 22, 415, 442
BstKTI GATC 3 cut(s) 88, 318, 854
BstMAI GTCTC 3 cut(s) 166, 429, 650
BstMBI GATC 3 cut(s) 85, 315, 851
BstMWI GCNNNNNNNGC 6 cut(s) 50, 166, 338, 437, 550, 798
BstNSI RCATGY 2 cut(s) 164, 524
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 504
BstUI CGCG 3 cut(s) 326, 440, 847
BstV1I GCAGC 4 cut(s) 518, 528, 681, 821
BstX2I RGATCY 2 cut(s) 85, 851
BstXI CCANNNNNNTGG 2 cut(s) 490, 639
BstYI RGATCY 2 cut(s) 85, 851
BsuRI GGCC 3 cut(s) 341, 427, 778
BtsCI GGATG 3 cut(s) 475, 632, 730
BtsIMutI CAGTG 3 cut(s) 427, 557, 769
Cac8I GCNNGC 7 cut(s) 24, 46, 162, 429, 444, 522, 615
CfoI GCGC 3 cut(s) 22, 415, 442
Cfr10I RCCGGY 2 cut(s) 22, 427
Cfr13I GGNCC 3 cut(s) 111, 425, 601
CseI GACGC 2 cut(s) 332, 446
Csp6I GTAC 1 cut(s) 418
CviAII CATG 2 cut(s) 161, 521
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 4 cut(s) 103, 139, 165, 536
DinI GGCGCC 1 cut(s) 21
DpnI GATC 3 cut(s) 87, 317, 853
DpnII GATC 3 cut(s) 85, 315, 851
EaeI YGGCCR 2 cut(s) 339, 776
EciI GGCGGA 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 50, 772
Eco47I GGWCC 2 cut(s) 111, 601
Eco53kI GAGCTC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 2 cut(s) 50, 772
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
Esp3I CGTCTC 2 cut(s) 166, 429
FaeI CATG 2 cut(s) 164, 524
FatI CATG 2 cut(s) 160, 520
FbaI TGATCA 1 cut(s) 315
Fnu4HI GCNGC 5 cut(s) 42, 507, 542, 670, 835
FokI GGATG 3 cut(s) 482, 619, 737
FriOI GRGCYC 2 cut(s) 50, 772
Fsp4HI GCNGC 5 cut(s) 42, 507, 542, 670, 835
FspBI CTAG 4 cut(s) 236, 626, 702, 855
FspI TGCGCA 1 cut(s) 414
GlaI GCGC 3 cut(s) 21, 414, 441
GluI GCNGC 5 cut(s) 42, 507, 542, 670, 835
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 3 cut(s) 341, 427, 778
HapII CCGG 4 cut(s) 17, 23, 36, 428
HgaI GACGC 2 cut(s) 332, 446
HhaI GCGC 3 cut(s) 22, 415, 442
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 2 cut(s) 164, 524
Hin6I GCGC 3 cut(s) 20, 413, 440
HinP1I GCGC 3 cut(s) 20, 413, 440
HpaII CCGG 4 cut(s) 17, 23, 36, 428
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 2 cut(s) 199, 498
Hpy188III TCNNGA 2 cut(s) 358, 796
Hpy8I GTNNAC 1 cut(s) 787
Hpy99I CGWCG 1 cut(s) 777
HpyAV CCTTC 5 cut(s) 171, 355, 564, 566, 615
HpyCH4III ACNGT 2 cut(s) 422, 737
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 3 cut(s) 125, 506, 686
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 166, 338, 437, 550, 798
HpyF3I CTNAG 4 cut(s) 103, 139, 165, 536
HpySE526I ACGT 1 cut(s) 376
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 2 cut(s) 164, 524
HspAI GCGC 3 cut(s) 20, 413, 440
KasI GGCGCC 1 cut(s) 19
KroI GCCGGC 2 cut(s) 22, 427
KroNI GCCGGC 2 cut(s) 24, 429
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 3 cut(s) 85, 315, 851
LmnI GCTCC 2 cut(s) 53, 798
Lsp1109I GCAGC 4 cut(s) 518, 528, 681, 821
LweI GCATC 3 cut(s) 355, 673, 695
MaeI CTAG 4 cut(s) 236, 626, 702, 855
MaeII ACGT 1 cut(s) 376
MalI GATC 3 cut(s) 87, 317, 853
MbiI CCGCTC 1 cut(s) 169
MboI GATC 3 cut(s) 85, 315, 851
MboII GAAGA 4 cut(s) 87, 90, 284, 581
MflI RGATCY 2 cut(s) 85, 851
MhlI GDGCHC 3 cut(s) 50, 772, 803
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 4 cut(s) 63, 98, 295, 352
MluI ACGCGT 1 cut(s) 845
MluNI TGGCCA 1 cut(s) 341
Mly113I GGCGCC 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 211, 549
Mox20I TGGCCA 1 cut(s) 341
Mph1103I ATGCAT 1 cut(s) 688
MroNI GCCGGC 2 cut(s) 22, 427
MscI TGGCCA 1 cut(s) 341
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 2 cut(s) 637, 762
Msp20I TGGCCA 1 cut(s) 341
MspI CCGG 4 cut(s) 17, 23, 36, 428
MspR9I CCNGG 1 cut(s) 17
MvnI CGCG 3 cut(s) 326, 440, 847
MwoI GCNNNNNNNGC 6 cut(s) 50, 166, 338, 437, 550, 798
NaeI GCCGGC 2 cut(s) 24, 429
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 1 cut(s) 17
NdeII GATC 3 cut(s) 85, 315, 851
NgoMIV GCCGGC 2 cut(s) 22, 427
NlaIII CATG 2 cut(s) 164, 524
NlaIV GGNNCC 2 cut(s) 21, 603
NsbI TGCGCA 1 cut(s) 414
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 2 cut(s) 164, 524
PaeI GCATGC 2 cut(s) 164, 524
PdiI GCCGGC 2 cut(s) 24, 429
PkrI GCNGC 5 cut(s) 43, 508, 543, 671, 836
PluTI GGCGCC 1 cut(s) 23
Ppu21I YACGTR 1 cut(s) 377
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 50
PspN4I GGNNCC 2 cut(s) 21, 603
PspPI GGNCC 3 cut(s) 111, 425, 601
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 2 cut(s) 85, 851
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 2 cut(s) 637, 762
SacI GAGCTC 1 cut(s) 50
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 5 cut(s) 42, 507, 542, 670, 835
Sau3AI GATC 3 cut(s) 85, 315, 851
Sau96I GGNCC 3 cut(s) 111, 425, 601
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 3 cut(s) 50, 772, 803
SetI ASST 7 cut(s) 50, 223, 379, 580, 619, 625, 831
SfaNI GCATC 3 cut(s) 355, 673, 695
SfcI CTRYAG 1 cut(s) 504
SfoI GGCGCC 1 cut(s) 21
SinI GGWCC 2 cut(s) 111, 601
SmiMI CAYNNNNRTG 2 cut(s) 637, 762
SphI GCATGC 2 cut(s) 164, 524
Sse9I AATT 4 cut(s) 63, 98, 295, 352
SsiI CCGC 6 cut(s) 41, 51, 169, 326, 382, 544
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 4 cut(s) 236, 626, 702, 855
SstI GAGCTC 1 cut(s) 50
StyD4I CCNGG 1 cut(s) 15
TaaI ACNGT 2 cut(s) 422, 737
TaiI ACGT 1 cut(s) 379
TaqI TCGA 1 cut(s) 772
TasI AATT 4 cut(s) 63, 98, 295, 352
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 3 cut(s) 427, 564, 769
TseI GCWGC 4 cut(s) 506, 541, 669, 834
TspDTI ATGAA 2 cut(s) 294, 411
TspGWI ACGGA 2 cut(s) 78, 625
TspRI CASTG 3 cut(s) 427, 564, 769
VpaK11BI GGWCC 2 cut(s) 111, 601
VspI ATTAAT 1 cut(s) 582
XceI RCATGY 2 cut(s) 164, 524
XspI CTAG 4 cut(s) 236, 626, 702, 855
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.