Rh1AG159400

Histidine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016956
Physical Location & Seq
Reverse (-)
30213163 .. 30225524
12362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG159400.1

Sequence Viewer

Length: 564 bp
ATGTCTCCTTTGAAGGAGGTTATCGACAGAATTCATGGTCAAGTTGCAAAGATTGAGGATGATCTCAAGGGTACTCGTGACTTTGCAAAAGAACAAATGACAATGAGAAAGAAGGCATTTTCGATAATAGAAGATGTTTTTGAGAGGCATGGTGCCACAGCCCTGGATACTCCAGTTTTTGAATTGAGAGACACTTTCATGGGCAAATATGGGGAAGACTCAAAATTGATTTATGATCTTGTTGACCAGGGAGGAGAGCTTTCCTCACTGCGATATGATTTCACTGTTCCATTTGCTAGAGATGGACTGTCACATAATTATTGTCCTCACCTTGTAAGAGACACCATCTTCCGGCCCTTTAGGTTCTTTTTGCTCACTGGGGACTTCTTATTAGCACCCAAAGAAAACTCCCAGGCAGCCATTCCTCCATTATCATTCCCTCTAAAAGCAGATAAAACAAATGGACCTCCACTACCGTCATATACATTATGCCCAAAAGCTTGCCTAAATGCCACTCCCAACCTCCGAACTGCATCACTATCCACCACACTCCCAGACGGTTGA

Protein Analysis

187

Amino Acids

20.94

Weight (kDa)

6.08

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_His PF13393 24 - 100 1.3e-07 Histidyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AcsI RAATTY 1 cut(s) 30
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 351
AgsI TTSAA 2 cut(s) 13, 182
AjnI CCWGG 3 cut(s) 162, 246, 411
AluBI AGCT 2 cut(s) 259, 500
AluI AGCT 2 cut(s) 259, 500
Alw26I GTCTC 3 cut(s) 9, 183, 333
AoxI GGCC 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 416
ApoI RAATTY 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 354, 464
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 464
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BanI GGYRCC 1 cut(s) 152
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 428
BccI CCATC 2 cut(s) 296, 353
BciT130I CCWGG 3 cut(s) 164, 248, 413
BciVI GTATCC 1 cut(s) 160
BcoDI GTCTC 3 cut(s) 9, 183, 333
BfaI CTAG 1 cut(s) 297
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 1 cut(s) 417
BlsI GCNGC 1 cut(s) 418
Bme1390I CCNGG 3 cut(s) 164, 248, 413
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 354, 464
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 3 cut(s) 164, 248, 413
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 1 cut(s) 542
BmuI ACTGGG 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 162, 247, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 351
Bse1I ACTGG 2 cut(s) 173, 382
BseBI CCWGG 3 cut(s) 164, 248, 413
BseDI CCNNGG 3 cut(s) 162, 247, 411
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 173, 382
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 428
BshFI GGCC 1 cut(s) 355
BshNI GGYRCC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 352
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 351
BsmAI GTCTC 3 cut(s) 9, 183, 333
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 1 cut(s) 355
Bsp143I GATC 2 cut(s) 61, 235
BspANI GGCC 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 152
BsrI ACTGG 2 cut(s) 173, 382
BssECI CCNNGG 3 cut(s) 162, 247, 411
BssMI GATC 2 cut(s) 61, 235
BssSI CACGAG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 164, 248, 413
Bst4CI ACNGT 4 cut(s) 286, 309, 477, 560
BstC8I GCNNGC 1 cut(s) 502
BstF5I GGATG 1 cut(s) 64
BstKTI GATC 2 cut(s) 64, 238
BstMAI GTCTC 3 cut(s) 9, 183, 333
BstMBI GATC 2 cut(s) 61, 235
BstNI CCWGG 3 cut(s) 164, 248, 413
BstSCI CCNGG 3 cut(s) 162, 246, 411
BstV1I GCAGC 1 cut(s) 428
BstV2I GAAGAC 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 163
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 355
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 3 cut(s) 266, 282, 375
Cac8I GCNNGC 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 354, 464
Csp6I GTAC 1 cut(s) 72
CviAII CATG 3 cut(s) 35, 149, 199
CviJI RGCY 5 cut(s) 161, 259, 355, 419, 500
CviKI_1 RGCY 5 cut(s) 161, 259, 355, 419, 500
CviQI GTAC 1 cut(s) 72
DpnI GATC 2 cut(s) 63, 237
DpnII GATC 2 cut(s) 61, 235
Eco47I GGWCC 1 cut(s) 464
EcoRI GAATTC 1 cut(s) 30
EcoRII CCWGG 3 cut(s) 162, 246, 411
FaeI CATG 3 cut(s) 38, 152, 202
FaqI GGGAC 1 cut(s) 395
FatI CATG 3 cut(s) 34, 148, 198
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 1 cut(s) 417
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 417
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 1 cut(s) 355
HapII CCGG 1 cut(s) 352
Hin1II CATG 3 cut(s) 38, 152, 202
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 1 cut(s) 218
HpaII CCGG 1 cut(s) 352
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 527
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 7, 106
HpyCH4III ACNGT 4 cut(s) 286, 309, 477, 560
HpyCH4V TGCA 3 cut(s) 47, 86, 533
Hsp92II CATG 3 cut(s) 38, 152, 202
Kzo9I GATC 2 cut(s) 61, 235
LpnPI CCDG 9 cut(s) 149, 176, 186, 233, 260, 363, 365, 398, 425
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 1 cut(s) 542
MaeI CTAG 1 cut(s) 297
MaeIII GTNAC 2 cut(s) 77, 309
MalI GATC 2 cut(s) 63, 237
MboI GATC 2 cut(s) 61, 235
MboII GAAGA 3 cut(s) 143, 227, 340
MluCI AATT 4 cut(s) 30, 182, 224, 316
MlyI GAGTC 1 cut(s) 212
MslI CAYNNNNRTG 1 cut(s) 197
MspI CCGG 1 cut(s) 352
MspR9I CCNGG 3 cut(s) 164, 248, 413
MvaI CCWGG 3 cut(s) 164, 248, 413
NdeII GATC 2 cut(s) 61, 235
NlaIII CATG 3 cut(s) 38, 152, 202
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 2 cut(s) 77, 309
PkrI GCNGC 1 cut(s) 418
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 3 cut(s) 162, 246, 411
PspGI CCWGG 3 cut(s) 162, 246, 411
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 2 cut(s) 354, 464
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
RseI CAYNNNNRTG 1 cut(s) 197
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 2 cut(s) 61, 235
Sau96I GGNCC 2 cut(s) 354, 464
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 3 cut(s) 164, 248, 413
SetI ASST 7 cut(s) 21, 261, 333, 365, 469, 502, 525
SfaNI GCATC 1 cut(s) 542
SinI GGWCC 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 4 cut(s) 30, 182, 224, 316
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 162, 246, 411
TaaI ACNGT 4 cut(s) 286, 309, 477, 560
TaqI TCGA 2 cut(s) 24, 122
TasI AATT 4 cut(s) 30, 182, 224, 316
TscAI CASTG 3 cut(s) 273, 289, 382
TseFI GTSAC 2 cut(s) 77, 309
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 2 cut(s) 77, 309
TspDTI ATGAA 2 cut(s) 23, 187
TspRI CASTG 3 cut(s) 273, 289, 382
VpaK11BI GGWCC 1 cut(s) 464
XapI RAATTY 1 cut(s) 30
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.