Rh1CG148600

Histidine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0016956
Physical Location & Seq
Reverse (-)
32722723 .. 32724594
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG148600.1

Sequence Viewer

Length: 366 bp
ATGTCTCCTTTGAAGGAGGTTATCGACAGAATTCATGGTCAAGTTGCAAAGATTGAGGATGATCTCAAGGGTACTCGTGACTTTGCAAAAGAACAAATGACAATGAGAAAGAAGGCATTTTCGATAATAGAAGATGTTTTTGAGAGGCATGGTGCCACAGCCCTGGATACTCCAGTTTTTGAATTGAGAGACACTTTCATGGGCAAATATGGGGAAGACTCAAAATTGATTTATGATCTTGTTGACCAGGGAGGAGAGCTTTCCTCACTGCGATATGATTTCACTGTTCCATTTGCTAGGTATGTTGCTATGAATGGCATCACATCCTTTAAAAGGTACCAGATTTCTAGGCTATCTATGTGCTAA

Protein Analysis

121

Amino Acids

13.93

Weight (kDa)

6.11

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_His PF13393 24 - 105 1.6e-09 Histidyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 152, 336
AcsI RAATTY 1 cut(s) 30
AfaI GTAC 2 cut(s) 73, 338
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 2 cut(s) 13, 182
AjnI CCWGG 2 cut(s) 162, 246
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
Alw26I GTCTC 2 cut(s) 9, 183
ApoI RAATTY 1 cut(s) 30
Asp718I GGTACC 1 cut(s) 336
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BanI GGYRCC 2 cut(s) 152, 336
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 222
BciT130I CCWGG 2 cut(s) 164, 248
BciVI GTATCC 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 9, 183
BfaI CTAG 2 cut(s) 297, 348
BfuI GTATCC 1 cut(s) 160
Bme1390I CCNGG 2 cut(s) 164, 248
BmiI GGNNCC 2 cut(s) 154, 338
BmrFI CCNGG 2 cut(s) 164, 248
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 162, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 2 cut(s) 164, 248
BseDI CCNNGG 2 cut(s) 162, 247
BseGI GGATG 2 cut(s) 64, 323
BseLI CCNNNNNNNGG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 267
BshNI GGYRCC 2 cut(s) 152, 336
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 2 cut(s) 9, 183
Bsp143I GATC 2 cut(s) 61, 235
BspLI GGNNCC 2 cut(s) 154, 338
BspT107I GGYRCC 2 cut(s) 152, 336
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 162, 247
BssMI GATC 2 cut(s) 61, 235
BssSI CACGAG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 164, 248
Bst4CI ACNGT 1 cut(s) 286
BstENI CCTNNNNNAGG 1 cut(s) 331
BstF5I GGATG 2 cut(s) 64, 323
BstKTI GATC 2 cut(s) 64, 238
BstMAI GTCTC 2 cut(s) 9, 183
BstMBI GATC 2 cut(s) 61, 235
BstNI CCWGG 2 cut(s) 164, 248
BstSCI CCNGG 2 cut(s) 162, 246
BstV2I GAAGAC 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 163
BsuI GTATCC 1 cut(s) 160
BtsCI GGATG 2 cut(s) 64, 323
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 2 cut(s) 266, 282
Csp6I GTAC 2 cut(s) 72, 337
CviAII CATG 3 cut(s) 35, 149, 199
CviJI RGCY 3 cut(s) 161, 259, 352
CviKI_1 RGCY 3 cut(s) 161, 259, 352
CviQI GTAC 2 cut(s) 72, 337
DpnI GATC 2 cut(s) 63, 237
DpnII GATC 2 cut(s) 61, 235
DraI TTTAAA 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 162, 246
FaeI CATG 3 cut(s) 38, 152, 202
FaiI YATR 9 cut(s) 36, 150, 200, 210, 234, 276, 303, 311, 359
FatI CATG 3 cut(s) 34, 148, 198
FokI GGATG 2 cut(s) 71, 310
FspBI CTAG 2 cut(s) 297, 348
GsuI CTGGAG 1 cut(s) 156
Hin1II CATG 3 cut(s) 38, 152, 202
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 1 cut(s) 218
Hpy166II GTNNAC 1 cut(s) 244
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 7, 106
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 2 cut(s) 47, 86
Hsp92II CATG 3 cut(s) 38, 152, 202
KpnI GGTACC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 61, 235
LpnPI CCDG 6 cut(s) 149, 176, 186, 233, 260, 353
LweI GCATC 1 cut(s) 327
MaeI CTAG 2 cut(s) 297, 348
MaeIII GTNAC 1 cut(s) 77
MalI GATC 2 cut(s) 63, 237
MboI GATC 2 cut(s) 61, 235
MboII GAAGA 2 cut(s) 143, 227
MluCI AATT 3 cut(s) 30, 182, 224
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 5 cut(s) 10, 49, 138, 245, 274
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 164, 248
MvaI CCWGG 2 cut(s) 164, 248
NdeII GATC 2 cut(s) 61, 235
NlaIII CATG 3 cut(s) 38, 152, 202
NlaIV GGNNCC 2 cut(s) 154, 338
NmuCI GTSAC 1 cut(s) 77
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 2 cut(s) 162, 246
PspGI CCWGG 2 cut(s) 162, 246
PspN4I GGNNCC 2 cut(s) 154, 338
RsaI GTAC 2 cut(s) 73, 338
RsaNI GTAC 2 cut(s) 72, 337
RseI CAYNNNNRTG 1 cut(s) 197
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 2 cut(s) 61, 235
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 164, 248
SetI ASST 4 cut(s) 21, 261, 302, 338
SfaNI GCATC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 3 cut(s) 30, 182, 224
SspMI CTAG 2 cut(s) 297, 348
StyD4I CCNGG 2 cut(s) 162, 246
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 2 cut(s) 24, 122
TasI AATT 3 cut(s) 30, 182, 224
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 2 cut(s) 273, 289
TseFI GTSAC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 23, 187, 326
TspRI CASTG 2 cut(s) 273, 289
XagI CCTNNNNNAGG 1 cut(s) 331
XapI RAATTY 1 cut(s) 30
XspI CTAG 2 cut(s) 297, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.