Rh7AG474400

Histidine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0016956
Physical Location & Seq
Forward (+)
65858605 .. 65860379
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG474400.1

Sequence Viewer

Length: 348 bp
ATGTCTCCTTTGAAGGAGGTTATCGACAGAATTCATGGTCAAGTTGCAAAGATTGAGGATGATCTCAAGGGTACTCGTGACTTTGCAAAAGAACAAATGACAATGAGAAAGAAGGAATTTTCGATAATAGAAGATGTTTCCGAGAGGCTTGGTGCCACAGCCCTGGATACTCCAGTTTTTGAATTGAGAGACACTCTCATGGGCAAATATGGGGAAGACTCAAAATTGATTTATGATCTTGTTGACCAGGGAGGAGAGCTTTCCTCACTACGATATGATTTCACTGTTCCATTTGCTAGGTATGTTGCTATGAATGGCATCACATCCTTTAAAAGGACAAGAGGCTAG

Protein Analysis

115

Amino Acids

13.1

Weight (kDa)

5.45

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_His PF13393 24 - 104 4.7e-07 Histidyl-tRNA synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AcsI RAATTY 2 cut(s) 30, 116
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 2 cut(s) 13, 182
AjnI CCWGG 2 cut(s) 162, 246
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
Alw26I GTCTC 2 cut(s) 9, 183
ApoI RAATTY 2 cut(s) 30, 116
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BanI GGYRCC 1 cut(s) 152
BauI CACGAG 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 222
BciT130I CCWGG 2 cut(s) 164, 248
BciVI GTATCC 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 9, 183
BfaI CTAG 2 cut(s) 297, 346
BfuI GTATCC 1 cut(s) 160
Bme1390I CCNGG 2 cut(s) 164, 248
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 2 cut(s) 164, 248
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 6 cut(s) 178, 210, 180, 212, 248, 280
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 162, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 2 cut(s) 164, 248
BseDI CCNNGG 2 cut(s) 162, 247
BseGI GGATG 2 cut(s) 64, 323
BseLI CCNNNNNNNGG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 2 cut(s) 9, 183
Bsp143I GATC 2 cut(s) 61, 235
BspLI GGNNCC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 152
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 162, 247
BssMI GATC 2 cut(s) 61, 235
BssSI CACGAG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 75
Bst2UI CCWGG 2 cut(s) 164, 248
Bst4CI ACNGT 1 cut(s) 286
BstENI CCTNNNNNAGG 1 cut(s) 331
BstF5I GGATG 2 cut(s) 64, 323
BstKTI GATC 2 cut(s) 64, 238
BstMAI GTCTC 2 cut(s) 9, 183
BstMBI GATC 2 cut(s) 61, 235
BstNI CCWGG 2 cut(s) 164, 248
BstSCI CCNGG 2 cut(s) 162, 246
BstV2I GAAGAC 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 163
BsuI GTATCC 1 cut(s) 160
BtsCI GGATG 2 cut(s) 64, 323
BtsIMutI CAGTG 1 cut(s) 282
Csp6I GTAC 1 cut(s) 72
CviAII CATG 2 cut(s) 35, 199
CviJI RGCY 4 cut(s) 148, 161, 259, 345
CviKI_1 RGCY 4 cut(s) 148, 161, 259, 345
CviQI GTAC 1 cut(s) 72
DpnI GATC 2 cut(s) 63, 237
DpnII GATC 2 cut(s) 61, 235
DraI TTTAAA 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 162, 246
FaeI CATG 2 cut(s) 38, 202
FaiI YATR 7 cut(s) 36, 200, 210, 234, 276, 303, 311
FatI CATG 2 cut(s) 34, 198
FokI GGATG 2 cut(s) 71, 310
FspBI CTAG 2 cut(s) 297, 346
GsuI CTGGAG 1 cut(s) 156
Hin1II CATG 2 cut(s) 38, 202
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 1 cut(s) 218
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 7, 106
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 2 cut(s) 47, 86
Hsp92II CATG 2 cut(s) 38, 202
Kzo9I GATC 2 cut(s) 61, 235
LpnPI CCDG 5 cut(s) 149, 176, 186, 233, 260
LweI GCATC 1 cut(s) 327
MaeI CTAG 2 cut(s) 297, 346
MaeIII GTNAC 1 cut(s) 77
MalI GATC 2 cut(s) 63, 237
MboI GATC 2 cut(s) 61, 235
MboII GAAGA 2 cut(s) 143, 227
MluCI AATT 4 cut(s) 30, 116, 182, 224
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 6 cut(s) 10, 49, 138, 245, 274, 335
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 164, 248
MvaI CCWGG 2 cut(s) 164, 248
NdeII GATC 2 cut(s) 61, 235
NlaIII CATG 2 cut(s) 38, 202
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 77
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 2 cut(s) 162, 246
PspGI CCWGG 2 cut(s) 162, 246
PspN4I GGNNCC 1 cut(s) 154
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
RseI CAYNNNNRTG 1 cut(s) 197
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 2 cut(s) 61, 235
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 164, 248
SetI ASST 3 cut(s) 21, 261, 302
SfaNI GCATC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 4 cut(s) 30, 116, 182, 224
SspMI CTAG 2 cut(s) 297, 346
StyD4I CCNGG 2 cut(s) 162, 246
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 2 cut(s) 24, 122
TasI AATT 4 cut(s) 30, 116, 182, 224
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 2 cut(s) 23, 326
TspRI CASTG 1 cut(s) 289
XagI CCTNNNNNAGG 1 cut(s) 331
XapI RAATTY 2 cut(s) 30, 116
XspI CTAG 2 cut(s) 297, 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.