Rh1AG212700

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016431
Physical Location & Seq
Forward (+)
40639626 .. 40645099
5474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG212700.1

Sequence Viewer

Length: 828 bp
ATGGTCTCAAGGCCAATATGCAGATATGTTGATCCATTGACTATAGGGTCGCATCCAATAGGAATTTTTGTACTTGGCCTCTCATATGATTTAGTTAGCTTCAGGTGGTTGTTGCTGGTGATTAACAAGGCTTTCGGGTATGCCATGTCGCATTCCAGATTATCCAAATGTTTCCTCGTGGTCGTAACAATCACTTCAAGGAGTGGAAATAACAAAAGATGTGCTCCAAGTCCTTGGGCTGTTGAACAGAATTCAACCACTGGAATGGATGGTACAAAGTCCTCCAGCTTTCCATACTTTTGGAAAACTTGCTGCTATCAAGGAGACGAAAAGCTTTGTGAAGCAAACGAAGAAAAGGGTCAGGGCCGTATGGTATGGCAGCTTGAGAACAAAGGTGGTGAGATGAACCTGGCTGCTGGCATTCAGGTGGCTCAATTGGCCCTCAAACATCGCCAGAATAAGAAGCAGCAACAAAGGATTATAGTCTTTGCTGGAAGTGTAGCCCTTGATATCATTAATTTTGGTGAAGAAGATGAAGAAAAGTCAGAGAAGCTAGAAGCACTTCTTGCTGCTGTGAACAATAATGACACCAGCCACATTGTTCATGTTTCGGCTGGTCCAAGTGCTCTTTCTGATGTACTCATAAGTACACCTATCTTCACTGGAGATGGAGAAGGTGGGAGTGGGATTAAGATTAAGTCTATTGTTGAAGCTTCAAAGCCGTGGCCATCGGTGAGCATGTCCAGGTGGTTACTCACGGTGAATCATGCCTTACCATTTGCCTGTGAGCCACATGTTATGGATGATTCCATTTGTTTGCAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

275

Amino Acids

30.18

Weight (kDa)

6.65

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_2 PF13519 127 - 165 2.6e-06 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 1 cut(s) 725
AcsI RAATTY 2 cut(s) 63, 250
AcuI CTGAAG 1 cut(s) 85
AfaI GTAC 4 cut(s) 72, 274, 639, 649
AflIII ACRYGT 1 cut(s) 793
AgsI TTSAA 5 cut(s) 198, 245, 255, 710, 717
AjnI CCWGG 2 cut(s) 408, 743
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 6 cut(s) 99, 288, 334, 382, 553, 713
AluI AGCT 6 cut(s) 99, 288, 334, 382, 553, 713
Alw21I GWGCWC 2 cut(s) 226, 628
Alw26I GTCTC 2 cut(s) 10, 318
AlwI GGATC 1 cut(s) 26
AoxI GGCC 5 cut(s) 11, 76, 364, 438, 725
ApeKI GCWGC 5 cut(s) 312, 379, 413, 466, 569
ApoI RAATTY 2 cut(s) 63, 250
AseI ATTAAT 1 cut(s) 516
Asp700I GAANNNNTTC 1 cut(s) 561
AspS9I GGNCC 3 cut(s) 364, 439, 617
AsuHPI GGTGA 5 cut(s) 130, 410, 536, 745, 772
AvaII GGWCC 1 cut(s) 617
BalI TGGCCA 1 cut(s) 727
BauI CACGAG 1 cut(s) 176
Bbv12I GWGCWC 2 cut(s) 226, 628
BbvI GCAGC 5 cut(s) 299, 391, 400, 478, 556
BccI CCATC 3 cut(s) 263, 662, 736
BceAI ACGGC 2 cut(s) 351, 706
BciT130I CCWGG 2 cut(s) 410, 745
BcoDI GTCTC 2 cut(s) 10, 318
BfaI CTAG 1 cut(s) 554
BfmI CTRYAG 1 cut(s) 42
BisI GCNGC 5 cut(s) 313, 380, 414, 467, 570
BlsI GCNGC 5 cut(s) 314, 381, 415, 468, 571
Bme1390I CCNGG 2 cut(s) 410, 745
Bme18I GGWCC 1 cut(s) 617
BmgT120I GGNCC 3 cut(s) 364, 439, 617
BmrFI CCNGG 2 cut(s) 410, 745
BmsI GCATC 1 cut(s) 61
BpmI CTGGAG 2 cut(s) 268, 684
BpuEI CTTGAG 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 233, 722
Bse1I ACTGG 2 cut(s) 265, 667
BseBI CCWGG 2 cut(s) 410, 745
BseDI CCNNGG 2 cut(s) 233, 722
BseGI GGATG 3 cut(s) 52, 274, 808
BseNI ACTGG 2 cut(s) 265, 667
BseXI GCAGC 5 cut(s) 299, 391, 400, 478, 556
BshFI GGCC 5 cut(s) 13, 78, 366, 440, 727
BsiHKAI GWGCWC 2 cut(s) 226, 628
BsmAI GTCTC 2 cut(s) 10, 318
BsmBI CGTCTC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 151, 420
BsnI GGCC 5 cut(s) 13, 78, 366, 440, 727
Bso31I GGTCTC 1 cut(s) 10
Bsp1286I GDGCHC 2 cut(s) 226, 628
Bsp143I GATC 1 cut(s) 31
BspANI GGCC 5 cut(s) 13, 78, 366, 440, 727
BspPI GGATC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 2 cut(s) 265, 667
BssECI CCNNGG 2 cut(s) 233, 722
BssMI GATC 1 cut(s) 31
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 233
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 410, 745
Bst4CI ACNGT 1 cut(s) 760
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 418
BstDSI CCRYGG 1 cut(s) 722
BstF5I GGATG 3 cut(s) 52, 274, 808
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 2 cut(s) 10, 318
BstMBI GATC 1 cut(s) 31
BstMWI GCNNNNNNNGC 2 cut(s) 437, 566
BstNI CCWGG 2 cut(s) 410, 745
BstNSI RCATGY 2 cut(s) 742, 797
BstSCI CCNGG 2 cut(s) 408, 743
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 5 cut(s) 299, 391, 400, 478, 556
BstXI CCANNNNNNTGG 3 cut(s) 234, 265, 300
BsuRI GGCC 5 cut(s) 13, 78, 366, 440, 727
BtgI CCRYGG 1 cut(s) 722
BtgZI GCGATG 1 cut(s) 434
BtsCI GGATG 3 cut(s) 52, 274, 808
BtsIMutI CAGTG 2 cut(s) 258, 660
Cac8I GCNNGC 1 cut(s) 418
Cfr13I GGNCC 3 cut(s) 364, 439, 617
Csp6I GTAC 4 cut(s) 71, 273, 638, 648
CviAII CATG 5 cut(s) 145, 605, 739, 767, 794
CviQI GTAC 4 cut(s) 71, 273, 638, 648
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 725
Eco130I CCWWGG 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 10
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 617
Eco57I CTGAAG 1 cut(s) 85
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 408, 743
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 5 cut(s) 148, 608, 742, 770, 797
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FatI CATG 5 cut(s) 144, 604, 738, 766, 793
FauNDI CATATG 1 cut(s) 85
Fnu4HI GCNGC 5 cut(s) 313, 380, 414, 467, 570
FokI GGATG 3 cut(s) 39, 281, 815
Fsp4HI GCNGC 5 cut(s) 313, 380, 414, 467, 570
FspBI CTAG 1 cut(s) 554
GluI GCNGC 5 cut(s) 313, 380, 414, 467, 570
GsuI CTGGAG 2 cut(s) 268, 684
HaeIII GGCC 5 cut(s) 13, 78, 366, 440, 727
Hin1II CATG 5 cut(s) 148, 608, 742, 770, 797
HindIII AAGCTT 2 cut(s) 332, 711
HinfI GANTC 2 cut(s) 763, 806
HphI GGTGA 5 cut(s) 130, 410, 536, 745, 772
Hpy166II GTNNAC 2 cut(s) 577, 650
Hpy188I TCNGA 2 cut(s) 547, 634
Hpy188III TCNNGA 1 cut(s) 156
Hpy8I GTNNAC 2 cut(s) 577, 650
HpyAV CCTTC 1 cut(s) 668
HpyCH4III ACNGT 1 cut(s) 760
HpyCH4V TGCA 2 cut(s) 21, 820
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 566
Hsp92II CATG 5 cut(s) 148, 608, 742, 770, 797
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 5 cut(s) 299, 391, 400, 478, 556
LweI GCATC 1 cut(s) 61
MaeI CTAG 1 cut(s) 554
MaeIII GTNAC 2 cut(s) 184, 750
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 5 cut(s) 362, 539, 542, 548, 649
MfeI CAATTG 1 cut(s) 434
MhlI GDGCHC 2 cut(s) 226, 628
MlsI TGGCCA 1 cut(s) 727
MluCI AATT 4 cut(s) 63, 250, 434, 517
MluNI TGGCCA 1 cut(s) 727
MnlI CCTC 4 cut(s) 89, 185, 292, 452
Mox20I TGGCCA 1 cut(s) 727
MroXI GAANNNNTTC 1 cut(s) 561
MscI TGGCCA 1 cut(s) 727
MseI TTAA 4 cut(s) 123, 516, 690, 696
MslI CAYNNNNRTG 2 cut(s) 263, 425
Msp20I TGGCCA 1 cut(s) 727
MspR9I CCNGG 2 cut(s) 410, 745
MunI CAATTG 1 cut(s) 434
Mva1269I GAATGC 2 cut(s) 151, 420
MvaI CCWGG 2 cut(s) 410, 745
MwoI GCNNNNNNNGC 2 cut(s) 437, 566
NdeI CATATG 1 cut(s) 85
NdeII GATC 1 cut(s) 31
NlaIII CATG 5 cut(s) 148, 608, 742, 770, 797
NspI RCATGY 2 cut(s) 742, 797
PciI ACATGT 1 cut(s) 793
PctI GAATGC 2 cut(s) 151, 420
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 2 cut(s) 763, 806
PkrI GCNGC 5 cut(s) 314, 381, 415, 468, 571
PscI ACATGT 1 cut(s) 793
PshBI ATTAAT 1 cut(s) 516
Psp6I CCWGG 2 cut(s) 408, 743
PspGI CCWGG 2 cut(s) 408, 743
PspPI GGNCC 3 cut(s) 364, 439, 617
RsaI GTAC 4 cut(s) 72, 274, 639, 649
RsaNI GTAC 4 cut(s) 71, 273, 638, 648
RseI CAYNNNNRTG 2 cut(s) 263, 425
SaqAI TTAA 4 cut(s) 123, 516, 690, 696
SatI GCNGC 5 cut(s) 313, 380, 414, 467, 570
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 3 cut(s) 364, 439, 617
ScrFI CCNGG 2 cut(s) 410, 745
SduI GDGCHC 2 cut(s) 226, 628
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 42
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 2 cut(s) 263, 425
SmlI CTYRAG 2 cut(s) 7, 383
SmoI CTYRAG 2 cut(s) 7, 383
Sse9I AATT 4 cut(s) 63, 250, 434, 517
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 2 cut(s) 408, 743
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 1 cut(s) 760
TasI AATT 4 cut(s) 63, 250, 434, 517
TatI WGTACW 3 cut(s) 70, 637, 647
TfiI GAWTC 2 cut(s) 763, 806
Tru1I TTAA 4 cut(s) 123, 516, 690, 696
Tru9I TTAA 4 cut(s) 123, 516, 690, 696
TscAI CASTG 2 cut(s) 265, 667
TseI GCWGC 5 cut(s) 312, 379, 413, 466, 569
TspDTI ATGAA 3 cut(s) 419, 549, 593
TspRI CASTG 2 cut(s) 265, 667
VpaK11BI GGWCC 1 cut(s) 617
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 2 cut(s) 63, 250
XceI RCATGY 2 cut(s) 742, 797
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.