Rorug03G0292000

26S proteasome non-atpase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004851
Physical Location & Seq
Reverse (-)
29858487 .. 29861910
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0292000.1

Sequence Viewer

Length: 1041 bp
ATGGTTGTACCATCTCCTTCTCCGATGAGAACCAAGAAAACCAAAGCAGTAGGGATTCCCACCATCGATCTTTCGAACTACAACCGGTCGAAGCTAGCGGAACTGGTCGTCGAAGCCTGCGAAGAATATGGTTTCTTCAAGGTGGTGAACCATGGCGTTCCAAAAGAGGTCATTGCCAGGATAGAAGAAGAAGGGTTTGATTTTTTTGCCAAACCCGCGGCGGAGAAGCAACGAGCTGGCCCGGCTTCTCCTTTCGGTTATGGAAGCAAAAACATTGGCCGCAATGGCGACATGGGCGAGCTCGAGTACCTTCTTCTTCAATCCAATCCTCTCTCCATTTCTGAAACATCCAATGCCGTCTCCAATGACCCTACAAAATTCAGCTGCGTTGTAAACGACTACATAGAAGCAGTTAAAGAATTGGCATGTGAGGTTCTTGATCTGGTCGGAGAAGGTCTATGGGTTTCGGACAAGTCTGTATTCAGTGGCTTCATCAGAGACGTCCAGAGTGACTCGATTCTCCGGCTGAACCACTATCCACCACTGGTGGATAAGGACCCATCCGCAAAACTAGCTCATCCTTGTGCGAATAACAATACTAGGATTGGATTTGGAGAGCATTCCGACCCCCAGATTTTGACCATTTTGAGATCCAACGATGTCGGAGGCCTTCAGATTTCTCTTTATGATGGCTTGTGGGTTCCAGTCCCGCCGGACTCGAATGAGTTTTTTGTCATGGTCGGTGATGTCTTGCAGGCTTTGACGAATGGGCGGTTTCAGAGTGTGAGGCACAGGGCATTGGCTAACTCTGTAACGAAGCCAAGAATGTCTATGGTCTACTTTGGGGCACCATCCCTAAACGCATGGATCTCGCCCCTTCCTGACATGGTGTCTCCCCAGAAGCCAAGTCTGTATAAACCATTCACATGGGGTCAATACAAGAAAGCTGCATACACTACACGTTTGGGAGATTCTCGTCTTGACAATTTCAAAATTACAAATAATCCATCTACATGCTTGGACAATCTTGTAGATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0001101 GO:0001653 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009555 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009743 GO:0009744 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010015 GO:0010029 GO:0010033 GO:0010053 GO:0010054 GO:0010150 GO:0010498 GO:0016020 GO:0016043 GO:0016049 GO:0019538 GO:0019941 GO:0021700 GO:0022414 GO:0022607 GO:0022622 GO:0023052 GO:0030154 GO:0030163 GO:0031593 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0033993 GO:0034285 GO:0034622 GO:0035966 GO:0038023 GO:0040007 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043248 GO:0043632 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048229 GO:0048364 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048468 GO:0048469 GO:0048528 GO:0048580 GO:0048588 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051788 GO:0060089 GO:0060560 GO:0061458 GO:0065003 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080147 GO:0090558 GO:0090567 GO:0090627 GO:0090693 GO:0090696 GO:0090697 GO:0090698 GO:0097305 GO:0099402 GO:0140030 GO:1900140 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
UIM VWA_2

Protein Analysis

346

Amino Acids

38.17

Weight (kDa)

5.82

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 19 - 89 7.4e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 171 - 282 2.8e-25 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 847
AccI GTMKAC 1 cut(s) 837
AccII CGCG 1 cut(s) 218
AciI CCGC 8 cut(s) 98, 216, 218, 221, 280, 564, 710, 772
AclWI GGATC 2 cut(s) 645, 875
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 656
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 2 cut(s) 9, 308
AflIII ACRYGT 1 cut(s) 959
AgeI ACCGGT 1 cut(s) 84
AgsI TTSAA 3 cut(s) 139, 320, 991
AhdI GACNNNNNGTC 1 cut(s) 889
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 6 cut(s) 94, 236, 301, 384, 575, 947
AluI AGCT 6 cut(s) 94, 236, 301, 384, 575, 947
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 3 cut(s) 364, 492, 897
AlwI GGATC 2 cut(s) 645, 875
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 3 cut(s) 238, 277, 667
ApeKI GCWGC 2 cut(s) 384, 947
ApoI RAATTY 1 cut(s) 377
AsiGI ACCGGT 1 cut(s) 84
AspS9I GGNCC 2 cut(s) 239, 556
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 157, 755
AsuII TTCGAA 1 cut(s) 74
AsuNHI GCTAGC 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 556
BaeGI GKGCMC 1 cut(s) 850
BanI GGYRCC 1 cut(s) 847
BanII GRGCYC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 371, 934
BccI CCATC 6 cut(s) 19, 71, 568, 683, 859, 1015
BceAI ACGGC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 1 cut(s) 178
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 3 cut(s) 364, 492, 897
BfaI CTAG 3 cut(s) 95, 572, 600
BglI GCCNNNNNGGC 1 cut(s) 285
BisI GCNGC 4 cut(s) 219, 280, 385, 948
BlsI GCNGC 4 cut(s) 220, 281, 386, 949
Bme1390I CCNGG 2 cut(s) 178, 242
Bme18I GGWCC 1 cut(s) 556
BmeRI GACNNNNNGTC 1 cut(s) 889
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 239, 556
BmiI GGNNCC 3 cut(s) 558, 702, 849
BmrFI CCNGG 2 cut(s) 178, 242
BmtI GCTAGC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 2 cut(s) 151, 216
BsaWI WCCGGW 1 cut(s) 84
Bse118I RCCGGY 1 cut(s) 84
Bse1I ACTGG 3 cut(s) 108, 549, 704
Bse3DI GCAATG 2 cut(s) 171, 289
BseBI CCWGG 1 cut(s) 178
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 151, 216
BseGI GGATG 4 cut(s) 347, 560, 577, 851
BseMI GCAATG 2 cut(s) 171, 289
BseNI ACTGG 3 cut(s) 108, 549, 704
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 2 cut(s) 371, 934
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 3 cut(s) 240, 279, 669
BshNI GGYRCC 1 cut(s) 847
BshTI ACCGGT 1 cut(s) 84
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 4 cut(s) 85, 242, 523, 713
BslFI GGGAC 1 cut(s) 692
BsmAI GTCTC 3 cut(s) 364, 492, 897
BsmBI CGTCTC 2 cut(s) 364, 492
BsmFI GGGAC 1 cut(s) 692
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 3 cut(s) 240, 279, 669
BsoBI CYCGRG 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 303, 850
Bsp143I GATC 5 cut(s) 67, 439, 650, 867, 1033
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 8 cut(s) 98, 216, 218, 221, 280, 564, 710, 772
BspANI GGCC 3 cut(s) 240, 279, 669
BspDI ATCGAT 1 cut(s) 66
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 3 cut(s) 558, 702, 849
BspOI GCTAGC 1 cut(s) 98
BspPI GGATC 2 cut(s) 645, 875
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 847
BsrDI GCAATG 2 cut(s) 171, 289
BsrFI RCCGGY 1 cut(s) 84
BsrI ACTGG 3 cut(s) 108, 549, 704
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 2 cut(s) 151, 216
BssMI GATC 5 cut(s) 67, 439, 650, 867, 1033
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 501
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 5 cut(s) 96, 118, 238, 299, 756
BstDSI CCRYGG 2 cut(s) 151, 216
BstF5I GGATG 4 cut(s) 347, 560, 577, 851
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 5 cut(s) 70, 442, 653, 870, 1036
BstMAI GTCTC 3 cut(s) 364, 492, 897
BstMBI GATC 5 cut(s) 67, 439, 650, 867, 1033
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 5 cut(s) 215, 242, 285, 294, 572
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 2 cut(s) 429, 1017
BstSCI CCNGG 2 cut(s) 176, 240
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 371, 934
BstX2I RGATCY 2 cut(s) 650, 867
BstXI CCANNNNNNTGG 1 cut(s) 927
BstYI RGATCY 2 cut(s) 650, 867
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 240, 279, 669
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 2 cut(s) 151, 216
BtsCI GGATG 4 cut(s) 347, 560, 577, 851
BtsIMutI CAGTG 2 cut(s) 490, 542
Cac8I GCNNGC 5 cut(s) 96, 118, 238, 299, 756
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 239, 556
Cfr42I CCGCGG 1 cut(s) 219
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 2 cut(s) 8, 307
CspAI ACCGGT 1 cut(s) 84
CviAII CATG 8 cut(s) 152, 292, 426, 736, 864, 886, 927, 1014
CviQI GTAC 2 cut(s) 8, 307
DpnI GATC 5 cut(s) 69, 441, 652, 869, 1035
DpnII GATC 5 cut(s) 67, 439, 650, 867, 1033
DriI GACNNNNNGTC 1 cut(s) 889
EaeI YGGCCR 1 cut(s) 277
Eam1105I GACNNNNNGTC 1 cut(s) 889
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 151
Esp3I CGTCTC 2 cut(s) 364, 492
FaeI CATG 8 cut(s) 155, 295, 429, 739, 867, 889, 930, 1017
FaqI GGGAC 1 cut(s) 692
FatI CATG 8 cut(s) 151, 291, 425, 735, 863, 885, 926, 1013
FauI CCCGC 2 cut(s) 223, 717
FblI GTMKAC 1 cut(s) 837
Fnu4HI GCNGC 4 cut(s) 219, 280, 385, 948
FokI GGATG 4 cut(s) 334, 547, 564, 838
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 4 cut(s) 219, 280, 385, 948
FspBI CTAG 3 cut(s) 95, 572, 600
GluI GCNGC 4 cut(s) 219, 280, 385, 948
HaeIII GGCC 3 cut(s) 240, 279, 669
HapII CCGG 4 cut(s) 85, 242, 523, 713
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 8 cut(s) 155, 295, 429, 739, 867, 889, 930, 1017
HinfI GANTC 5 cut(s) 55, 512, 517, 716, 971
HpaII CCGG 4 cut(s) 85, 242, 523, 713
HphI GGTGA 2 cut(s) 157, 755
Hpy166II GTNNAC 3 cut(s) 148, 394, 838
Hpy188I TCNGA 9 cut(s) 24, 343, 449, 469, 497, 625, 665, 675, 780
Hpy188III TCNNGA 4 cut(s) 437, 505, 881, 980
Hpy8I GTNNAC 3 cut(s) 148, 394, 838
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 6 cut(s) 27, 185, 320, 446, 680, 887
HpyCH4IV ACGT 2 cut(s) 501, 961
HpyCH4V TGCA 2 cut(s) 754, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 242, 285, 294, 572
HpySE526I ACGT 2 cut(s) 501, 961
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 8 cut(s) 155, 295, 429, 739, 867, 889, 930, 1017
KspI CCGCGG 1 cut(s) 219
Kzo9I GATC 5 cut(s) 67, 439, 650, 867, 1033
Lsp1109I GCAGC 2 cut(s) 371, 934
MaeI CTAG 3 cut(s) 95, 572, 600
MaeII ACGT 2 cut(s) 501, 961
MaeIII GTNAC 2 cut(s) 509, 811
MalI GATC 5 cut(s) 69, 441, 652, 869, 1035
MboI GATC 5 cut(s) 67, 439, 650, 867, 1033
MboII GAAGA 6 cut(s) 127, 134, 197, 200, 305, 308
MflI RGATCY 2 cut(s) 650, 867
MhlI GDGCHC 2 cut(s) 303, 850
MluCI AATT 4 cut(s) 377, 419, 985, 993
MlyI GAGTC 2 cut(s) 506, 710
MmeI TCCRAC 4 cut(s) 427, 643, 648, 678
MnlI CCTC 5 cut(s) 160, 339, 424, 659, 780
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 3 cut(s) 582, 925, 1012
MspA1I CMGCKG 2 cut(s) 218, 384
MspI CCGG 4 cut(s) 85, 242, 523, 713
MspR9I CCNGG 2 cut(s) 178, 242
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 5 cut(s) 215, 242, 285, 294, 572
NciI CCSGG 1 cut(s) 242
NcoI CCATGG 1 cut(s) 151
NdeII GATC 5 cut(s) 67, 439, 650, 867, 1033
NheI GCTAGC 1 cut(s) 94
NlaIII CATG 8 cut(s) 155, 295, 429, 739, 867, 889, 930, 1017
NlaIV GGNNCC 3 cut(s) 558, 702, 849
NmuCI GTSAC 1 cut(s) 509
NspI RCATGY 2 cut(s) 429, 1017
NspV TTCGAA 1 cut(s) 74
PaeR7I CTCGAG 1 cut(s) 302
PceI AGGCCT 1 cut(s) 669
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 619
PfeI GAWTC 3 cut(s) 55, 517, 971
PinAI ACCGGT 1 cut(s) 84
PkrI GCNGC 4 cut(s) 220, 281, 386, 949
PleI GAGTC 2 cut(s) 506, 710
PpsI GAGTC 2 cut(s) 506, 710
PpuMI RGGWCCY 1 cut(s) 556
Psp124BI GAGCTC 1 cut(s) 303
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 558, 702, 849
PspPI GGNCC 2 cut(s) 239, 556
PspPPI RGGWCCY 1 cut(s) 556
PspXI VCTCGAGB 1 cut(s) 302
PsuI RGATCY 2 cut(s) 650, 867
PvuII CAGCTG 1 cut(s) 384
RsaI GTAC 2 cut(s) 9, 308
RsaNI GTAC 2 cut(s) 8, 307
RseI CAYNNNNRTG 3 cut(s) 582, 925, 1012
SacI GAGCTC 1 cut(s) 303
SacII CCGCGG 1 cut(s) 219
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 4 cut(s) 219, 280, 385, 948
Sau3AI GATC 5 cut(s) 67, 439, 650, 867, 1033
Sau96I GGNCC 2 cut(s) 239, 556
SchI GAGTC 2 cut(s) 506, 710
ScrFI CCNGG 2 cut(s) 178, 242
SduI GDGCHC 2 cut(s) 303, 850
Sfr274I CTCGAG 1 cut(s) 302
Sfr303I CCGCGG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 74
SgrBI CCGCGG 1 cut(s) 219
SinI GGWCC 1 cut(s) 556
SlaI CTCGAG 1 cut(s) 302
SmiMI CAYNNNNRTG 3 cut(s) 582, 925, 1012
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 4 cut(s) 377, 419, 985, 993
SseBI AGGCCT 1 cut(s) 669
SsiI CCGC 8 cut(s) 98, 216, 218, 221, 280, 564, 710, 772
SspMI CTAG 3 cut(s) 95, 572, 600
SstI GAGCTC 1 cut(s) 303
StuI AGGCCT 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 176, 240
StyI CCWWGG 1 cut(s) 151
TaiI ACGT 2 cut(s) 504, 964
TaqI TCGA 7 cut(s) 66, 74, 89, 111, 303, 515, 719
TasI AATT 4 cut(s) 377, 419, 985, 993
TauI GCSGC 2 cut(s) 221, 282
TfiI GAWTC 3 cut(s) 55, 517, 971
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 2 cut(s) 490, 549
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 2 cut(s) 384, 947
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 1 cut(s) 481
TspRI CASTG 2 cut(s) 490, 549
VpaK11BI GGWCC 1 cut(s) 556
XapI RAATTY 1 cut(s) 377
XceI RCATGY 2 cut(s) 429, 1017
XhoI CTCGAG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 837
XspI CTAG 3 cut(s) 95, 572, 600
ZraI GACGTC 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.