Rh1BG422000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004851
Physical Location & Seq
Forward (+)
54174038 .. 54187167
13130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG422000.1

Sequence Viewer

Length: 507 bp
ATGCTCCCTCACCAGCACTACATTGGGAAGCACCCTAAGAACCTCCCTCACCGAATCATAATCCCCGAGGCTAAGATTGAAACGTACGGATCCATGAGTACCATAGGAGACATCCAGCTGCACGCACTAGTTGTTGATGCCAATGAGGAACATGAAGCACTGCTCAAACAGGAGCCTCAAGTGGAGAGTGAAGAGGAGATACTTGAGGCAAACAAAGAACTCAATGAAGACGTAAAGCCCGAGCTCAAGGAATTTGCACGATGTGGTATTGAGTTGATCATTGACCATTCTTTTGTGGACTACACTTGTTCTAGCTCCACTGATAGTTGTCTGCAATGGGTTTCAATAACATCTACAACAAAGATACAGGTGCTTCAAAAAAATGAGGCCTTGACTGGACAAGAAGGCATTGATAGGGGAATGATCTGCGTCAATTTGTTTGTTTATTACCTTGACCCCTGGCAAGGATCCAAGGATAGCATGCTTGGTGAGCTTACAAGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

19.02

Weight (kDa)

4.71

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 84, 97, 462, 475
AcsI RAATTY 1 cut(s) 251
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 2 cut(s) 86, 100
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 80, 345, 377
AhlI ACTAGT 1 cut(s) 127
AjnI CCWGG 1 cut(s) 458
AluBI AGCT 5 cut(s) 118, 244, 315, 493, 501
AluI AGCT 5 cut(s) 118, 244, 315, 493, 501
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 4 cut(s) 84, 97, 462, 475
Ama87I CYCGRG 2 cut(s) 65, 239
AoxI GGCC 1 cut(s) 387
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AsuHPI GGTGA 2 cut(s) 41, 500
AvaI CYCGRG 2 cut(s) 65, 239
BamHI GGATCC 2 cut(s) 89, 467
BanII GRGCYC 1 cut(s) 246
BarI GAAGNNNNNNTAC 2 cut(s) 183, 215
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 105
BciT130I CCWGG 1 cut(s) 460
BclI TGATCA 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 102
BcuI ACTAGT 1 cut(s) 127
BfaI CTAG 2 cut(s) 128, 312
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
Bme1390I CCNGG 1 cut(s) 460
BmeT110I CYCGRG 2 cut(s) 65, 239
BmiI GGNNCC 3 cut(s) 91, 174, 469
BmrFI CCNGG 1 cut(s) 460
BmsI GCATC 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 234
BpuEI CTTGAG 3 cut(s) 162, 224, 230
BsaJI CCNNGG 3 cut(s) 66, 458, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse1I ACTGG 1 cut(s) 400
Bse3DI GCAATG 1 cut(s) 341
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 3 cut(s) 66, 458, 471
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 341
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 105
BsgI GTGCAG 1 cut(s) 104
BshFI GGCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 2 cut(s) 65, 239
BsiWI CGTACG 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 102
BsnI GGCC 1 cut(s) 389
BsoBI CYCGRG 2 cut(s) 65, 239
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 4 cut(s) 89, 276, 423, 467
BspANI GGCC 1 cut(s) 389
BspLI GGNNCC 3 cut(s) 91, 174, 469
BspPI GGATC 4 cut(s) 84, 97, 462, 475
BsrDI GCAATG 1 cut(s) 341
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 3 cut(s) 66, 458, 471
BssMI GATC 4 cut(s) 89, 276, 423, 467
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 123, 482
BstDEI CTNAG 2 cut(s) 36, 72
BstF5I GGATG 1 cut(s) 111
BstKTI GATC 4 cut(s) 92, 279, 426, 470
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 4 cut(s) 89, 276, 423, 467
BstMWI GCNNNNNNNGC 1 cut(s) 490
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 1 cut(s) 484
BstSCI CCNGG 1 cut(s) 458
BstV1I GCAGC 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 2 cut(s) 89, 467
BstYI RGATCY 2 cut(s) 89, 467
BsuRI GGCC 1 cut(s) 389
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 2 cut(s) 158, 318
Cac8I GCNNGC 2 cut(s) 123, 482
CseI GACGC 1 cut(s) 418
Csp6I GTAC 2 cut(s) 85, 99
CviAII CATG 3 cut(s) 94, 152, 481
CviJI RGCY 9 cut(s) 71, 118, 175, 238, 244, 315, 389, 493, 501
CviKI_1 RGCY 9 cut(s) 71, 118, 175, 238, 244, 315, 389, 493, 501
CviQI GTAC 2 cut(s) 85, 99
DdeI CTNAG 2 cut(s) 36, 72
DpnI GATC 4 cut(s) 91, 278, 425, 469
DpnII GATC 4 cut(s) 89, 276, 423, 467
DraIII CACNNNGTG 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 389
Eco24I GRGCYC 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 244
Eco88I CYCGRG 2 cut(s) 65, 239
EcoICRI GAGCTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 3 cut(s) 97, 155, 484
FaiI YATR 5 cut(s) 59, 95, 104, 153, 482
FatI CATG 3 cut(s) 93, 151, 480
FbaI TGATCA 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 119
FspBI CTAG 2 cut(s) 128, 312
GluI GCNGC 1 cut(s) 119
HaeIII GGCC 1 cut(s) 389
HgaI GACGC 1 cut(s) 418
Hin1II CATG 3 cut(s) 97, 155, 484
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 1 cut(s) 54
HphI GGTGA 2 cut(s) 41, 500
Hpy166II GTNNAC 1 cut(s) 298
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 1 cut(s) 398
HpyCH4IV ACGT 2 cut(s) 83, 231
HpyCH4V TGCA 3 cut(s) 121, 257, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 490
HpyF3I CTNAG 2 cut(s) 36, 72
HpySE526I ACGT 2 cut(s) 83, 231
Hsp92II CATG 3 cut(s) 97, 155, 484
Ksp22I TGATCA 1 cut(s) 276
Kzo9I GATC 4 cut(s) 89, 276, 423, 467
LmnI GCTCC 3 cut(s) 9, 172, 320
LpnPI CCDG 7 cut(s) 26, 128, 155, 353, 381, 445, 472
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 128, 312
MaeII ACGT 2 cut(s) 83, 231
MalI GATC 4 cut(s) 91, 278, 425, 469
MboI GATC 4 cut(s) 89, 276, 423, 467
MboII GAAGA 2 cut(s) 203, 239
MflI RGATCY 2 cut(s) 89, 467
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 2 cut(s) 251, 433
MnlI CCTC 9 cut(s) 18, 53, 57, 61, 139, 186, 187, 199, 379
MspA1I CMGCKG 1 cut(s) 118
MspR9I CCNGG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 1 cut(s) 490
NdeII GATC 4 cut(s) 89, 276, 423, 467
NlaIII CATG 3 cut(s) 97, 155, 484
NlaIV GGNNCC 3 cut(s) 91, 174, 469
NspI RCATGY 1 cut(s) 484
PaeI GCATGC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 54
Pfl23II CGTACG 1 cut(s) 84
PkrI GCNGC 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 458
PspGI CCWGG 1 cut(s) 458
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 91, 174, 469
PsuI RGATCY 2 cut(s) 89, 467
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 2 cut(s) 86, 100
RsaNI GTAC 2 cut(s) 85, 99
SacI GAGCTC 1 cut(s) 246
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 4 cut(s) 89, 276, 423, 467
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 1 cut(s) 127
SmlI CTYRAG 3 cut(s) 177, 203, 245
SmoI CTYRAG 3 cut(s) 177, 203, 245
SpeI ACTAGT 1 cut(s) 127
SphI GCATGC 1 cut(s) 484
Sse9I AATT 2 cut(s) 251, 433
SseBI AGGCCT 1 cut(s) 389
SspMI CTAG 2 cut(s) 128, 312
SstI GAGCTC 1 cut(s) 246
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 471
TaiI ACGT 2 cut(s) 86, 234
TasI AATT 2 cut(s) 251, 433
TfiI GAWTC 1 cut(s) 54
TscAI CASTG 2 cut(s) 165, 325
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 168, 240
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 2 cut(s) 165, 325
XapI RAATTY 1 cut(s) 251
XceI RCATGY 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
XspI CTAG 2 cut(s) 128, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.