Rh2AG022700

Chlororespiratory reduction 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012411
Physical Location & Seq
Reverse (-)
1618892 .. 1622668
3777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG022700.1

Sequence Viewer

Length: 612 bp
ATGTATCAACTGTCTCAGACCATATCAGTTCATAGCTCACACCCCTTTGCAATGGCTGCAATCTTCCAAACTCTGCTCCCCATTTGTTCTTCTTCAAGCTCAAGGCAGACTCCATGGAATACTCCCTCCAGACCCATCTCAGATTCCATGGCCTCTTTGCCCATTTCACACTTCTATAGGCAGCAAGGGCAAGTAGCAGTCTCAGTTGCCTTCAACCCTTCTGGGAATTTTGACCTCCCTCTATATGATGATGAAGAAGACACATCCAAGGTTGAGCCTCCAATGCCACCAAGTGAAGGCAGATTTGAGATTGTTATTGACAATGATGTAATTCGTCGTCTCGACTTAACTCCATTTCATACTGTAACTGGCATTACTAATCCTTCATCAGCCAAGCCAAAAGAGTACCTTGAGCGTACAATTGGCTTTACCATTAATTACACAAGAGAAGATCCTCGCGATCCTCGAGAGCTATCCGAGTTTCCTGATATAAGGCTATGGTTTTTGAGACTTGATGCTGCTTATCCTTGGTTGCCTGTGCTCTTGGATTGGCGAGCCGGAGAGCTAGCTCGTTATGCTGCTATGTTGGTGCCACATCAGGTAATAGACTAA

Protein Analysis

203

Amino Acids

23.08

Weight (kDa)

5.07

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Crr6 PF08847 106 - 201 2.6e-35 Chlororespiratory reduction 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47910 AT2G47910
fragaria_vesca FvH4_1g01870
malus_domestica MD02G1016600.v1.1 MD15G1161100.v1.1
prunus_persica Prupe.7G256300_v2.0.a1
pyrus_communis pycom02g01470 pycom02g01490
rosa_chinensis RchiOBHm_Chr2g0086841
rosa_laevigata RLG00000015809
rosa_multiflora Rmu_sc0007954.1_g000001
rosa_roxburghii Rroxscaffold_2G00154090
rosa_rugosa Rorug01G0470100.1 Rorug01G0470200
rosa_samantha Rh2AG022700 Rh2BG023500 Rh2CG023700 Rh2DG023300
rosa_wichuraiana Rw2G001800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 589
AccII CGCG 1 cut(s) 459
AclWI GGATC 2 cut(s) 446, 455
AcsI RAATTY 1 cut(s) 226
AdeI CACNNNGTG 1 cut(s) 293
AfaI GTAC 2 cut(s) 407, 418
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 2 cut(s) 96, 214
AluBI AGCT 5 cut(s) 36, 99, 472, 565, 569
AluI AGCT 5 cut(s) 36, 99, 472, 565, 569
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 4 cut(s) 18, 205, 344, 502
AlwI GGATC 2 cut(s) 446, 455
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 4 cut(s) 56, 181, 518, 578
ApoI RAATTY 1 cut(s) 226
AseI ATTAAT 1 cut(s) 435
AsuNHI GCTAGC 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 465
BanI GGYRCC 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 4 cut(s) 43, 193, 505, 565
BccI CCATC 1 cut(s) 143
BcoDI GTCTC 4 cut(s) 18, 205, 344, 502
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 4 cut(s) 57, 182, 519, 579
BlsI GCNGC 4 cut(s) 58, 183, 520, 580
BmeT110I CYCGRG 1 cut(s) 465
BmiI GGNNCC 1 cut(s) 591
BmsI GCATC 1 cut(s) 505
BmtI GCTAGC 1 cut(s) 569
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 112
BpuEI CTTGAG 2 cut(s) 85, 431
BsaJI CCNNGG 4 cut(s) 113, 147, 267, 527
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 57
BseDI CCNNGG 4 cut(s) 113, 147, 267, 527
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 3 cut(s) 29, 153, 216
BseNI ACTGG 1 cut(s) 373
BseXI GCAGC 4 cut(s) 43, 193, 505, 565
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 543
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 1 cut(s) 558
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 4 cut(s) 18, 205, 344, 502
BsmBI CGTCTC 1 cut(s) 344
BsnI GGCC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 2 cut(s) 451, 460
Bsp19I CCATGG 2 cut(s) 113, 147
Bsp68I TCGCGA 1 cut(s) 459
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 3 cut(s) 28, 152, 215
BspFNI CGCG 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 591
BspOI GCTAGC 1 cut(s) 569
BspPI GGATC 2 cut(s) 446, 455
BspT107I GGYRCC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 57
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 4 cut(s) 113, 147, 267, 527
BssMI GATC 2 cut(s) 451, 460
BssT1I CCWWGG 4 cut(s) 113, 147, 267, 527
Bst4CI ACNGT 2 cut(s) 12, 364
BstAPI GCANNNNNTGC 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 555, 567
BstDEI CTNAG 3 cut(s) 15, 139, 202
BstDSI CCRYGG 2 cut(s) 113, 147
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 459
BstKTI GATC 2 cut(s) 454, 463
BstMAI GTCTC 4 cut(s) 18, 205, 344, 502
BstMBI GATC 2 cut(s) 451, 460
BstMWI GCNNNNNNNGC 4 cut(s) 56, 187, 283, 575
BstSFI CTRYAG 1 cut(s) 175
BstUI CGCG 1 cut(s) 459
BstV1I GCAGC 4 cut(s) 43, 193, 505, 565
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 2 cut(s) 113, 147
BtsCI GGATG 1 cut(s) 263
BtuMI TCGCGA 1 cut(s) 459
Cac8I GCNNGC 2 cut(s) 555, 567
Csp6I GTAC 2 cut(s) 406, 417
CviAII CATG 2 cut(s) 114, 148
CviQI GTAC 2 cut(s) 406, 417
DdeI CTNAG 3 cut(s) 15, 139, 202
DpnI GATC 2 cut(s) 453, 462
DpnII GATC 2 cut(s) 451, 460
DraIII CACNNNGTG 1 cut(s) 293
Eco130I CCWWGG 4 cut(s) 113, 147, 267, 527
Eco88I CYCGRG 1 cut(s) 465
EcoT14I CCWWGG 4 cut(s) 113, 147, 267, 527
ErhI CCWWGG 4 cut(s) 113, 147, 267, 527
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 2 cut(s) 117, 151
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 2 cut(s) 113, 147
Fnu4HI GCNGC 4 cut(s) 57, 182, 519, 579
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 4 cut(s) 57, 182, 519, 579
FspBI CTAG 1 cut(s) 566
GluI GCNGC 4 cut(s) 57, 182, 519, 579
GsuI CTGGAG 1 cut(s) 112
HaeIII GGCC 1 cut(s) 152
HapII CCGG 1 cut(s) 558
Hin1II CATG 2 cut(s) 117, 151
HinfI GANTC 2 cut(s) 109, 143
HpaII CCGG 1 cut(s) 558
Hpy188I TCNGA 3 cut(s) 18, 142, 478
Hpy188III TCNNGA 5 cut(s) 129, 341, 458, 467, 485
Hpy99I CGWCG 1 cut(s) 339
HpyAV CCTTC 4 cut(s) 220, 228, 290, 393
HpyCH4III ACNGT 2 cut(s) 12, 364
HpyCH4V TGCA 2 cut(s) 50, 59
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 187, 283, 575
HpyF3I CTNAG 3 cut(s) 15, 139, 202
Hsp92II CATG 2 cut(s) 117, 151
Kzo9I GATC 2 cut(s) 451, 460
LmnI GCTCC 1 cut(s) 81
LpnPI CCDG 7 cut(s) 142, 207, 354, 498, 549, 571, 584
Lsp1109I GCAGC 4 cut(s) 43, 193, 505, 565
LweI GCATC 1 cut(s) 505
MaeI CTAG 1 cut(s) 566
MaeIII GTNAC 1 cut(s) 364
MalI GATC 2 cut(s) 453, 462
MboI GATC 2 cut(s) 451, 460
MboII GAAGA 6 cut(s) 55, 81, 84, 266, 269, 461
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 543
MluCI AATT 4 cut(s) 226, 330, 420, 436
MlyI GAGTC 1 cut(s) 103
MnlI CCTC 7 cut(s) 136, 163, 245, 249, 288, 465, 474
MseI TTAA 2 cut(s) 347, 435
MspI CCGG 1 cut(s) 558
MunI CAATTG 1 cut(s) 420
MvnI CGCG 1 cut(s) 459
MwoI GCNNNNNNNGC 4 cut(s) 56, 187, 283, 575
NcoI CCATGG 2 cut(s) 113, 147
NdeII GATC 2 cut(s) 451, 460
NheI GCTAGC 1 cut(s) 565
NlaIII CATG 2 cut(s) 117, 151
NlaIV GGNNCC 1 cut(s) 591
NruI TCGCGA 1 cut(s) 459
PaeR7I CTCGAG 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PfeI GAWTC 1 cut(s) 143
PkrI GCNGC 4 cut(s) 58, 183, 520, 580
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 435
PspN4I GGNNCC 1 cut(s) 591
PsuI RGATCY 1 cut(s) 451
RruI TCGCGA 1 cut(s) 459
RsaI GTAC 2 cut(s) 407, 418
RsaNI GTAC 2 cut(s) 406, 417
SaqAI TTAA 2 cut(s) 347, 435
SatI GCNGC 4 cut(s) 57, 182, 519, 579
Sau3AI GATC 2 cut(s) 451, 460
SchI GAGTC 1 cut(s) 103
SduI GDGCHC 1 cut(s) 543
SetI ASST 9 cut(s) 38, 101, 237, 273, 411, 474, 567, 571, 603
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 175
Sfr274I CTCGAG 1 cut(s) 465
SlaI CTCGAG 1 cut(s) 465
SmlI CTYRAG 3 cut(s) 100, 410, 465
SmoI CTYRAG 3 cut(s) 100, 410, 465
Sse9I AATT 4 cut(s) 226, 330, 420, 436
SspMI CTAG 1 cut(s) 566
StyI CCWWGG 4 cut(s) 113, 147, 267, 527
TaaI ACNGT 2 cut(s) 12, 364
TaqI TCGA 2 cut(s) 342, 466
TasI AATT 4 cut(s) 226, 330, 420, 436
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 2 cut(s) 347, 435
Tru9I TTAA 2 cut(s) 347, 435
TseI GCWGC 4 cut(s) 56, 181, 518, 578
TspDTI ATGAA 4 cut(s) 20, 267, 347, 375
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 465
XspI CTAG 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.