Rh2CG023700

Chlororespiratory reduction 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0012411
Physical Location & Seq
Reverse (-)
1799203 .. 1802970
3768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG023700.1

Sequence Viewer

Length: 771 bp
ATGGCTGCAATCTTCCAAACACTGCTCCCCATTTGTTCTTCTTCAAGCTCAAGGCAGACTCCATGGAATACTCCCTCCAGACCCATCTCAGATTCCATGGCCTCTTTGCCCATTTCACACTTATATAGGCAGCAAGGGCAAGTAGCAGTCTCAGTTGCCTTCAACCCTTCTGGGAATTTTGACCTCCCTCTATATGATGATGAAGAAGACACATCCAAGGTTGAGCCTCCAATGCCACCAAGTGAAGGCAGATTTGAGATTGTTATTGACAATGATGTAATTCGTCGTCTCGACTTAACTCCATTTCATACTGTAACTGGCATTACTAATCCTTCATCAGCCAAGCCAAAAGAGTACATTGAGCGTACAATTGGCTTTACCATTAATTACACAAGAGAAGATCCTCGCGATCCTCGAGAGCTATCCGAGTTTCCTGATATAAGGCTATGGTTTTTGAGACTTGATGCTGCTTATCCTTGGTTGCCTGTGCTCTTGGATTGGCGAGCCGGAGAGCTAGCTCGTTATGCTGCTATGTTGGTGCCACATCAGATGAATATGAGGATGGGTGTTGTGTTCAATCCTGAGGCACTAGAACTGTTTGTTATGCAGAAAGTCTTTGTTGTGTACTTGTGGCTGAAGCAACACAACATTCCAAAGCCTAGATTGAAGACTAGTGACATGGCTAGAATGCTCGGGTTTGGTATTGGAGATGAGCTTTATGATTTGATTGATAAACATCCGATTTCCACATCATCAAAACACAAAGCCTAA

Protein Analysis

256

Amino Acids

29.25

Weight (kDa)

5.88

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Crr6 PF08847 89 - 242 2.9e-59 Chlororespiratory reduction 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47910 AT2G47910
fragaria_vesca FvH4_1g01870
malus_domestica MD02G1016600.v1.1 MD15G1161100.v1.1
prunus_persica Prupe.7G256300_v2.0.a1
pyrus_communis pycom02g01470 pycom02g01490
rosa_chinensis RchiOBHm_Chr2g0086841
rosa_laevigata RLG00000015809
rosa_multiflora Rmu_sc0007954.1_g000001
rosa_roxburghii Rroxscaffold_2G00154090
rosa_rugosa Rorug01G0470100.1 Rorug01G0470200
rosa_samantha Rh2AG022700 Rh2BG023500 Rh2CG023700 Rh2DG023300
rosa_wichuraiana Rw2G001800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AccII CGCG 1 cut(s) 408
AclWI GGATC 2 cut(s) 395, 404
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 1 cut(s) 656
AdeI CACNNNGTG 1 cut(s) 242
AfaI GTAC 3 cut(s) 356, 367, 626
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 4 cut(s) 45, 163, 577, 667
AhlI ACTAGT 1 cut(s) 671
AluBI AGCT 5 cut(s) 48, 421, 514, 518, 715
AluI AGCT 5 cut(s) 48, 421, 514, 518, 715
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 3 cut(s) 154, 293, 451
AlwI GGATC 2 cut(s) 395, 404
Ama87I CYCGRG 2 cut(s) 414, 692
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 4 cut(s) 5, 130, 467, 527
ApoI RAATTY 1 cut(s) 175
AseI ATTAAT 1 cut(s) 384
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 2 cut(s) 414, 692
AxyI CCTNAGG 1 cut(s) 582
BanI GGYRCC 1 cut(s) 538
BbsI GAAGAC 2 cut(s) 213, 674
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 3 cut(s) 142, 454, 514
BccI CCATC 2 cut(s) 92, 556
BcoDI GTCTC 3 cut(s) 154, 293, 451
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 5 cut(s) 515, 590, 660, 672, 684
BisI GCNGC 4 cut(s) 6, 131, 468, 528
BlsI GCNGC 4 cut(s) 7, 132, 469, 529
BmeT110I CYCGRG 2 cut(s) 414, 692
BmiI GGNNCC 1 cut(s) 540
BmsI GCATC 1 cut(s) 454
BmtI GCTAGC 1 cut(s) 518
BpiI GAAGAC 2 cut(s) 213, 674
BpmI CTGGAG 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 735
BsaJI CCNNGG 4 cut(s) 62, 96, 216, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 322
Bse21I CCTNAGG 1 cut(s) 582
Bse8I GATNNNNATC 1 cut(s) 735
BseDI CCNNGG 4 cut(s) 62, 96, 216, 476
BseGI GGATG 3 cut(s) 212, 567, 736
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMII CTCAG 3 cut(s) 102, 165, 573
BseNI ACTGG 1 cut(s) 322
BseXI GCAGC 3 cut(s) 142, 454, 514
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 2 cut(s) 414, 692
BsiSI CCGG 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 3 cut(s) 154, 293, 451
BsmBI CGTCTC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 1 cut(s) 101
BsoBI CYCGRG 2 cut(s) 414, 692
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 400, 409
Bsp19I CCATGG 2 cut(s) 62, 96
Bsp68I TCGCGA 1 cut(s) 408
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 3 cut(s) 101, 164, 574
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 540
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 2 cut(s) 395, 404
BspT107I GGYRCC 1 cut(s) 538
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 4 cut(s) 62, 96, 216, 476
BssMI GATC 2 cut(s) 400, 409
BssT1I CCWWGG 4 cut(s) 62, 96, 216, 476
Bst4CI ACNGT 2 cut(s) 313, 597
BstC8I GCNNGC 2 cut(s) 504, 516
BstDEI CTNAG 3 cut(s) 88, 151, 582
BstDSI CCRYGG 2 cut(s) 62, 96
BstF5I GGATG 3 cut(s) 212, 567, 736
BstFNI CGCG 1 cut(s) 408
BstKTI GATC 2 cut(s) 403, 412
BstMAI GTCTC 3 cut(s) 154, 293, 451
BstMBI GATC 2 cut(s) 400, 409
BstMWI GCNNNNNNNGC 3 cut(s) 136, 232, 524
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 3 cut(s) 142, 454, 514
BstV2I GAAGAC 2 cut(s) 213, 674
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
Bsu36I CCTNAGG 1 cut(s) 582
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 2 cut(s) 62, 96
BtsCI GGATG 3 cut(s) 212, 567, 736
BtsI GCAGTG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 20
BtuMI TCGCGA 1 cut(s) 408
Cac8I GCNNGC 2 cut(s) 504, 516
Csp6I GTAC 3 cut(s) 355, 366, 625
CviAII CATG 3 cut(s) 63, 97, 679
CviQI GTAC 3 cut(s) 355, 366, 625
DdeI CTNAG 3 cut(s) 88, 151, 582
DpnI GATC 2 cut(s) 402, 411
DpnII GATC 2 cut(s) 400, 409
DraIII CACNNNGTG 1 cut(s) 242
Eco130I CCWWGG 4 cut(s) 62, 96, 216, 476
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 582
Eco88I CYCGRG 2 cut(s) 414, 692
EcoT14I CCWWGG 4 cut(s) 62, 96, 216, 476
ErhI CCWWGG 4 cut(s) 62, 96, 216, 476
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 3 cut(s) 66, 100, 682
FatI CATG 3 cut(s) 62, 96, 678
Fnu4HI GCNGC 4 cut(s) 6, 131, 468, 528
FokI GGATG 3 cut(s) 199, 574, 723
Fsp4HI GCNGC 4 cut(s) 6, 131, 468, 528
FspBI CTAG 5 cut(s) 515, 590, 660, 672, 684
GluI GCNGC 4 cut(s) 6, 131, 468, 528
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 507
Hin1II CATG 3 cut(s) 66, 100, 682
HinfI GANTC 2 cut(s) 58, 92
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 4 cut(s) 91, 427, 549, 741
Hpy188III TCNNGA 6 cut(s) 78, 290, 407, 416, 434, 581
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 4 cut(s) 169, 177, 239, 342
HpyCH4III ACNGT 2 cut(s) 313, 597
HpyCH4V TGCA 2 cut(s) 8, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 232, 524
HpyF3I CTNAG 3 cut(s) 88, 151, 582
Hsp92II CATG 3 cut(s) 66, 100, 682
Kzo9I GATC 2 cut(s) 400, 409
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 7 cut(s) 91, 156, 303, 447, 498, 520, 594
Lsp1109I GCAGC 3 cut(s) 142, 454, 514
LweI GCATC 1 cut(s) 454
MaeI CTAG 5 cut(s) 515, 590, 660, 672, 684
MaeIII GTNAC 2 cut(s) 313, 674
MalI GATC 2 cut(s) 402, 411
MboI GATC 2 cut(s) 400, 409
MboII GAAGA 7 cut(s) 4, 30, 33, 215, 218, 410, 679
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 4 cut(s) 175, 279, 369, 385
MlyI GAGTC 1 cut(s) 52
MnlI CCTC 9 cut(s) 85, 112, 194, 198, 237, 414, 423, 552, 577
MseI TTAA 2 cut(s) 296, 384
MspI CCGG 1 cut(s) 507
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 693
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 3 cut(s) 136, 232, 524
NcoI CCATGG 2 cut(s) 62, 96
NdeII GATC 2 cut(s) 400, 409
NheI GCTAGC 1 cut(s) 514
NlaIII CATG 3 cut(s) 66, 100, 682
NlaIV GGNNCC 1 cut(s) 540
NmuCI GTSAC 1 cut(s) 674
NruI TCGCGA 1 cut(s) 408
PaeR7I CTCGAG 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PctI GAATGC 1 cut(s) 693
PfeI GAWTC 1 cut(s) 92
PkrI GCNGC 4 cut(s) 7, 132, 469, 529
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PshBI ATTAAT 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 540
PsuI RGATCY 1 cut(s) 400
RruI TCGCGA 1 cut(s) 408
RsaI GTAC 3 cut(s) 356, 367, 626
RsaNI GTAC 3 cut(s) 355, 366, 625
SaqAI TTAA 2 cut(s) 296, 384
SatI GCNGC 4 cut(s) 6, 131, 468, 528
Sau3AI GATC 2 cut(s) 400, 409
SchI GAGTC 1 cut(s) 52
SduI GDGCHC 1 cut(s) 492
SetI ASST 7 cut(s) 50, 186, 222, 423, 516, 520, 717
SfaNI GCATC 1 cut(s) 454
Sfr274I CTCGAG 1 cut(s) 414
SlaI CTCGAG 1 cut(s) 414
SmlI CTYRAG 2 cut(s) 49, 414
SmoI CTYRAG 2 cut(s) 49, 414
SpeI ACTAGT 1 cut(s) 671
Sse9I AATT 4 cut(s) 175, 279, 369, 385
SspMI CTAG 5 cut(s) 515, 590, 660, 672, 684
StyI CCWWGG 4 cut(s) 62, 96, 216, 476
TaaI ACNGT 2 cut(s) 313, 597
TaqI TCGA 2 cut(s) 291, 415
TasI AATT 4 cut(s) 175, 279, 369, 385
TatI WGTACW 2 cut(s) 354, 624
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 2 cut(s) 296, 384
Tru9I TTAA 2 cut(s) 296, 384
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 4 cut(s) 5, 130, 467, 527
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 4 cut(s) 216, 296, 324, 566
TspRI CASTG 1 cut(s) 27
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 414
XspI CTAG 5 cut(s) 515, 590, 660, 672, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.