Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0009938

Physical Location & Seq

Genomic Coordinates Forward (+)

38545936 .. 38554387

Gene Structure 8452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG305900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGAGCAGCCAAGTTGGTCTGAGGAGGACCAGTCTCACCCAGAAGCGCGGCCCCTCCACCGTCACTAAGAAATCGGTCCCAAGCGAGAACGGAGCCATCTCTAATGGCACCACCAGCAAACCCTCGTCTCCTCCTGCCCTTGCGACTGCTGGTGGAGAGAGGACAGTGAAGAAGCTGAGGTTGTCGAAAGCCCTCACAATCCCCGAGGGGACCACTGTATCCGATGCTTGCCGGAGGATGGCTGCTCGGCGTGTGGATGCTGTGCTTTTGACTGATGCAAATGCATTGCTTTCTGGAATTGTGACTGACAAGGACATTGCCAGTAGGGTCATTGCAGAGGGATTGAGACCGGAGCAGACAATTGTATCAAAGATCATGACGCGGAATCCTATTTTTGTTAATGCTGACTCATTGGCCATGGAAGCTCTACAGAAGATGATCCAGGGTAAATTTAGGCACCTTCCTGTTGTAGAGAATGGTGAGGTTATTGCCTTGTTGGATATCACAAAATGCCTCTATGATGCTATATCAAGAATGGAAAAGGCAGCTGAGCAGGGGAGTGCCATTGCTGCTGCAGTTGAAGGAGTTGAACGCCAGTGGGGAGCCAATTTTTCTGCTCCATATGCTTTTCTGGAAACTCTGAGGGAGCGAATGTTCAAGCCTTCATTGTCAACCATCATTGGTGAAAACACAAAGGTTGCAATTGTATCCCCATCAGATCCTGTCTATGTTGCGGCAAAAAGGATGCGGGACTTTCGGGTTAATTCAGTTATAATTGTCATGGGGAACAAGATTCAAGGGATACTCACTTCAAAGGATATTTTAATGCGAGTCGTGGCACAAAATCTTTCTCCTGAGTTGACTCTGGTGGAAAAGGTAATGACGCCGAACCCTGAATGTGCTACATTAGAGACTACAATTCTTGATGCACTGCATATAATGCATGAAGGGAAGTTCTTGCATCTTCCTGTCTTAGACAGAGATGAATCTGTTGCTGCATGTGTGGATGTTCTGCAGATAACTCACGCGGCAATCTCTATGGTTGAAAGTAGTTCAGGAAGCGTTAATGATATGGCAAACACAATGATGCAAAAGTTCTGGGATTCAGCACTTGCTTTAGAGCCGGCTGATGACTGTGATACTCAAAGTGAAATGTCTGCACTAATGGCTTCTGATGCGGCAGAACAAGCCAAGTTTACTTATCCTTCTATTGGTCTTGGAAATTCATTCGCCTTCAAGTTTGAAGATCGAAAAGGTCGAGTGCATCGACTCAATAGTGGCACAGAAAATTTAGATGAGCTTGTATCTGCTGTCATGCGAAGGACTGGTGGTGCTAAGGATAATGATCGTCCTCAGATTTTGTATGAAGATGATGAAGGTGATAGAGTTCTACTTGCAAGTGATGATGATCTTGTTAGCGCTGTCAGCCATGCTCGAGCAGTGGGACAGAAGGTAGTGTTTATGCTTCATATATTATTTTTTTCTAGAGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS PB1

Protein Analysis

497

Amino Acids

53.94

Weight (kDa)

6.14

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBS	PF00571	62 - 110	3.3e-07	CBS domain
CBS	PF00571	122 - 168	2.7e-09	CBS domain
CBS	PF00571	230 - 278	8.7e-07	CBS domain
CBS	PF00571	290 - 329	6.6e-06	CBS domain
PB1	PF00564	410 - 489	1.1e-12	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009938)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52950 AT3G52950
fragaria_vesca	FvH4_1g25510 FvH4_1g25510
malus_domestica	MD08G1219400.v1.1 MD15G1408000.v1.1
prunus_persica	Prupe.1G553500_v2.0.a1
pyrus_communis	pycom08g19040 pycom15g36410
rosa_chinensis	RchiOBHm_Chr2g0123641
rosa_laevigata	RLG00000018765
rosa_multiflora	Rmu_co8197906.1_g000001 Rmu_sc0004915.1_g000016
rosa_roxburghii	Rroxscaffold_2G00120490
rosa_rugosa	Rorug02G0246700 Rorug02G0246800
rosa_samantha	Rh2AG305900 Rh2BG313900 Rh2CG292200 Rh2DG329700
rosa_wichuraiana	Rw2G024530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	773
AccB1I	GGYRCC	2 cut(s)	107, 456
AccII	CGCG	3 cut(s)	48, 382, 1028
AciI	CCGC	6 cut(s)	48, 382, 734, 748, 1028, 1178
AclWI	GGATC	2 cut(s)	433, 713
AcoI	YGGCCR	1 cut(s)	414
AcsI	RAATTY	3 cut(s)	449, 1222, 1288
AcyI	GRCGYC	1 cut(s)	884
AfeI	AGCGCT	1 cut(s)	1420
AfiI	CCNNNNNNNGG	2 cut(s)	238, 1211
AgsI	TTSAA	8 cut(s)	581, 590, 658, 797, 813, 1046, 1237, 1244
AjnI	CCWGG	1 cut(s)	441
AloI	GAACNNNNNNTCC	2 cut(s)	638, 670
AluBI	AGCT	4 cut(s)	175, 425, 548, 1300
AluI	AGCT	4 cut(s)	175, 425, 548, 1300
Alw26I	GTCTC	4 cut(s)	38, 132, 340, 905
AlwI	GGATC	2 cut(s)	433, 713
AlwNI	CAGNNNCTG	1 cut(s)	722
Ama87I	CYCGRG	2 cut(s)	203, 1434
Aor51HI	AGCGCT	1 cut(s)	1420
AoxI	GGCC	2 cut(s)	49, 414
ApeKI	GCWGC	6 cut(s)	6, 242, 545, 569, 572, 995
ApoI	RAATTY	3 cut(s)	449, 1222, 1288
AspLEI	GCGC	2 cut(s)	48, 1421
AspS9I	GGNCC	4 cut(s)	27, 50, 76, 210
AsuHPI	GGTGA	4 cut(s)	28, 491, 695, 1391
AvaI	CYCGRG	2 cut(s)	203, 1434
AvaII	GGWCC	3 cut(s)	27, 76, 210
BaeI	ACNNNNGTAYC	4 cut(s)	201, 234, 348, 381
BalI	TGGCCA	1 cut(s)	416
BanI	GGYRCC	2 cut(s)	107, 456
BbvCI	CCTCAGC	1 cut(s)	176
BbvI	GCAGC	6 cut(s)	18, 229, 556, 557, 559, 982
BccI	CCATC	4 cut(s)	104, 232, 683, 721
BciT130I	CCWGG	1 cut(s)	443
BciVI	GTATCC	3 cut(s)	229, 718, 795
BcoDI	GTCTC	4 cut(s)	38, 132, 340, 905
BfaI	CTAG	1 cut(s)	1485
BfmI	CTRYAG	3 cut(s)	428, 573, 1013
BfoI	RGCGCY	1 cut(s)	1422
BfuI	GTATCC	3 cut(s)	229, 718, 795
BlpI	GCTNAGC	1 cut(s)	549
Bme1390I	CCNGG	1 cut(s)	443
Bme18I	GGWCC	3 cut(s)	27, 76, 210
BmeT110I	CYCGRG	2 cut(s)	203, 1434
BmgT120I	GGNCC	4 cut(s)	27, 50, 76, 210
BmiI	GGNNCC	7 cut(s)	52, 78, 94, 109, 211, 458, 604
BmrFI	CCNGG	1 cut(s)	443
Bpu10I	CCTNAGC	2 cut(s)	176, 1335
Bpu1102I	GCTNAGC	1 cut(s)	549
BsaBI	GATNNNNATC	2 cut(s)	1344, 1407
BsaHI	GRCGYC	1 cut(s)	884
BsaI	GGTCTC	1 cut(s)	340
BsaJI	CCNNGG	3 cut(s)	204, 417, 442
BsaWI	WCCGGW	1 cut(s)	349
BsaXI	ACNNNNNCTCC	4 cut(s)	16, 46, 638, 668
Bsc4I	CCNNNNNNNGG	2 cut(s)	238, 1211
Bse118I	RCCGGY	1 cut(s)	1123
Bse1I	ACTGG	4 cut(s)	30, 321, 595, 1330
Bse3DI	GCAATG	4 cut(s)	284, 315, 330, 564
Bse8I	GATNNNNATC	2 cut(s)	1344, 1407
BseBI	CCWGG	1 cut(s)	443
BseDI	CCNNGG	3 cut(s)	204, 417, 442
BseGI	GGATG	4 cut(s)	243, 262, 750, 1012
BseJI	GATNNNNATC	2 cut(s)	1344, 1407
BseLI	CCNNNNNNNGG	2 cut(s)	238, 1211
BseMI	GCAATG	4 cut(s)	284, 315, 330, 564
BseMII	CTCAG	6 cut(s)	11, 167, 540, 632, 846, 1367
BseNI	ACTGG	4 cut(s)	30, 321, 595, 1330
BseRI	GAGGAG	2 cut(s)	37, 120
BseXI	GCAGC	6 cut(s)	18, 229, 556, 557, 559, 982
BsgI	GTGCAG	1 cut(s)	1143
Bsh1236I	CGCG	3 cut(s)	48, 382, 1028
BshFI	GGCC	2 cut(s)	51, 416
BshNI	GGYRCC	2 cut(s)	107, 456
BsiHKCI	CYCGRG	2 cut(s)	203, 1434
BsiSI	CCGG	3 cut(s)	232, 350, 1124
BslFI	GGGAC	4 cut(s)	62, 223, 764, 1458
BslI	CCNNNNNNNGG	2 cut(s)	238, 1211
BsmAI	GTCTC	4 cut(s)	38, 132, 340, 905
BsmBI	CGTCTC	1 cut(s)	132
BsmFI	GGGAC	4 cut(s)	62, 223, 764, 1458
BsnI	GGCC	2 cut(s)	51, 416
Bso31I	GGTCTC	1 cut(s)	340
BsoBI	CYCGRG	2 cut(s)	203, 1434
Bsp143I	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
Bsp1720I	GCTNAGC	1 cut(s)	549
Bsp19I	CCATGG	1 cut(s)	417
BspACI	CCGC	6 cut(s)	48, 382, 734, 748, 1028, 1178
BspANI	GGCC	2 cut(s)	51, 416
BspCNI	CTCAG	6 cut(s)	12, 168, 541, 633, 847, 1366
BspFNI	CGCG	3 cut(s)	48, 382, 1028
BspHI	TCATGA	1 cut(s)	375
BspLI	GGNNCC	7 cut(s)	52, 78, 94, 109, 211, 458, 604
BspMAI	CTGCAG	2 cut(s)	577, 1017
BspPI	GGATC	2 cut(s)	433, 713
BspT107I	GGYRCC	2 cut(s)	107, 456
BspTNI	GGTCTC	1 cut(s)	340
BsrDI	GCAATG	4 cut(s)	284, 315, 330, 564
BsrFI	RCCGGY	1 cut(s)	1123
BsrI	ACTGG	4 cut(s)	30, 321, 595, 1330
BssAI	RCCGGY	1 cut(s)	1123
BssECI	CCNNGG	3 cut(s)	204, 417, 442
BssMI	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
BssNI	GRCGYC	1 cut(s)	884
BssT1I	CCWWGG	1 cut(s)	417
Bst2UI	CCWGG	1 cut(s)	443
Bst4CI	ACNGT	4 cut(s)	61, 166, 217, 1136
BstACI	GRCGYC	1 cut(s)	884
BstAPI	GCANNNNNTGC	1 cut(s)	940
BstC8I	GCNNGC	2 cut(s)	229, 1125
BstDEI	CTNAG	9 cut(s)	20, 66, 176, 549, 641, 855, 973, 1335, 1353
BstDSI	CCRYGG	1 cut(s)	417
BstF5I	GGATG	4 cut(s)	243, 262, 750, 1012
BstFNI	CGCG	3 cut(s)	48, 382, 1028
BstH2I	RGCGCY	1 cut(s)	1422
BstHHI	GCGC	2 cut(s)	48, 1421
BstKTI	GATC	6 cut(s)	375, 441, 721, 1249, 1348, 1411
BstMAI	GTCTC	4 cut(s)	38, 132, 340, 905
BstMBI	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
BstMWI	GCNNNNNNNGC	9 cut(s)	114, 422, 569, 623, 940, 1166, 1175, 1187, 1425
BstNI	CCWGG	1 cut(s)	443
BstNSI	RCATGY	1 cut(s)	1002
BstSCI	CCNGG	1 cut(s)	441
BstSFI	CTRYAG	3 cut(s)	428, 573, 1013
BstUI	CGCG	3 cut(s)	48, 382, 1028
BstV1I	GCAGC	6 cut(s)	18, 229, 556, 557, 559, 982
BstX2I	RGATCY	1 cut(s)	718
BstYI	RGATCY	1 cut(s)	718
BsuI	GTATCC	3 cut(s)	229, 718, 795
BsuRI	GGCC	2 cut(s)	51, 416
BtgI	CCRYGG	1 cut(s)	417
BtsCI	GGATG	4 cut(s)	243, 262, 750, 1012
BtsI	GCAGTG	2 cut(s)	929, 1446
BtsIMutI	CAGTG	5 cut(s)	171, 213, 602, 929, 1446
Cac8I	GCNNGC	2 cut(s)	229, 1125
CaiI	CAGNNNCTG	1 cut(s)	722
CciI	TCATGA	1 cut(s)	375
CfoI	GCGC	2 cut(s)	48, 1421
Cfr10I	RCCGGY	1 cut(s)	1123
Cfr13I	GGNCC	4 cut(s)	27, 50, 76, 210
CseI	GACGC	2 cut(s)	388, 892
CviAII	CATG	7 cut(s)	376, 418, 781, 944, 999, 1315, 1430
DdeI	CTNAG	9 cut(s)	20, 66, 176, 549, 641, 855, 973, 1335, 1353
DpnI	GATC	6 cut(s)	374, 440, 720, 1248, 1347, 1410
DpnII	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
EaeI	YGGCCR	1 cut(s)	414
Eco130I	CCWWGG	1 cut(s)	417
Eco31I	GGTCTC	1 cut(s)	340
Eco32I	GATATC	1 cut(s)	502
Eco47I	GGWCC	3 cut(s)	27, 76, 210
Eco47III	AGCGCT	1 cut(s)	1420
Eco88I	CYCGRG	2 cut(s)	203, 1434
EcoRII	CCWGG	1 cut(s)	441
EcoRV	GATATC	1 cut(s)	502
EcoT14I	CCWWGG	1 cut(s)	417
EcoT22I	ATGCAT	2 cut(s)	286, 945
ErhI	CCWWGG	1 cut(s)	417
Esp3I	CGTCTC	1 cut(s)	132
FaeI	CATG	7 cut(s)	379, 421, 784, 947, 1002, 1318, 1433
FaqI	GGGAC	4 cut(s)	62, 223, 764, 1458
FatI	CATG	7 cut(s)	375, 417, 780, 943, 998, 1314, 1429
FauI	CCCGC	1 cut(s)	741
FauNDI	CATATG	1 cut(s)	622
FokI	GGATG	4 cut(s)	250, 269, 757, 1019
FspBI	CTAG	1 cut(s)	1485
GlaI	GCGC	2 cut(s)	47, 1420
HaeII	RGCGCY	1 cut(s)	1422
HaeIII	GGCC	2 cut(s)	51, 416
HapII	CCGG	3 cut(s)	232, 350, 1124
HgaI	GACGC	2 cut(s)	388, 892
HhaI	GCGC	2 cut(s)	48, 1421
Hin1I	GRCGYC	1 cut(s)	884
Hin1II	CATG	7 cut(s)	379, 421, 784, 947, 1002, 1318, 1433
Hin6I	GCGC	2 cut(s)	46, 1419
HinP1I	GCGC	2 cut(s)	46, 1419
HincII	GTYRAC	2 cut(s)	672, 861
HindII	GTYRAC	2 cut(s)	672, 861
HinfI	GANTC	8 cut(s)	385, 407, 793, 831, 862, 986, 1103, 1269
HpaII	CCGG	3 cut(s)	232, 350, 1124
HphI	GGTGA	4 cut(s)	28, 491, 695, 1391
Hpy166II	GTNNAC	3 cut(s)	672, 861, 1197
Hpy188I	TCNGA	6 cut(s)	21, 223, 642, 718, 1174, 1356
Hpy188III	TCNNGA	8 cut(s)	294, 376, 531, 632, 854, 923, 1056, 1485
Hpy8I	GTNNAC	3 cut(s)	672, 861, 1197
HpyAV	CCTTC	9 cut(s)	470, 575, 672, 941, 1215, 1243, 1314, 1370, 1444
HpyCH4III	ACNGT	4 cut(s)	61, 166, 217, 1136
HpyF10VI	GCNNNNNNNGC	9 cut(s)	114, 422, 569, 623, 940, 1166, 1175, 1187, 1425
HpyF3I	CTNAG	9 cut(s)	20, 66, 176, 549, 641, 855, 973, 1335, 1353
Hsp92I	GRCGYC	1 cut(s)	884
Hsp92II	CATG	7 cut(s)	379, 421, 784, 947, 1002, 1318, 1433
HspAI	GCGC	2 cut(s)	46, 1419
KroI	GCCGGC	1 cut(s)	1123
KroNI	GCCGGC	1 cut(s)	1125
Kzo9I	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
LmnI	GCTCC	5 cut(s)	92, 352, 602, 622, 646
Lsp1109I	GCAGC	6 cut(s)	18, 229, 556, 557, 559, 982
MaeI	CTAG	1 cut(s)	1485
MaeIII	GTNAC	2 cut(s)	61, 301
MalI	GATC	6 cut(s)	374, 440, 720, 1248, 1347, 1410
MboI	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
MboII	GAAGA	5 cut(s)	181, 445, 956, 1256, 1379
MfeI	CAATTG	2 cut(s)	360, 702
MflI	RGATCY	1 cut(s)	718
MlsI	TGGCCA	1 cut(s)	416
MluNI	TGGCCA	1 cut(s)	416
MlyI	GAGTC	4 cut(s)	401, 840, 856, 1263
MmeI	TCCRAC	1 cut(s)	477
Mox20I	TGGCCA	1 cut(s)	416
Mph1103I	ATGCAT	2 cut(s)	286, 945
MroNI	GCCGGC	1 cut(s)	1123
MscI	TGGCCA	1 cut(s)	416
MseI	TTAA	5 cut(s)	399, 762, 824, 1065, 1492
MslI	CAYNNNNRTG	1 cut(s)	1085
Msp20I	TGGCCA	1 cut(s)	416
MspA1I	CMGCKG	1 cut(s)	548
MspI	CCGG	3 cut(s)	232, 350, 1124
MspR9I	CCNGG	1 cut(s)	443
MunI	CAATTG	2 cut(s)	360, 702
MvaI	CCWGG	1 cut(s)	443
MvnI	CGCG	3 cut(s)	48, 382, 1028
MwoI	GCNNNNNNNGC	9 cut(s)	114, 422, 569, 623, 940, 1166, 1175, 1187, 1425
NaeI	GCCGGC	1 cut(s)	1125
NcoI	CCATGG	1 cut(s)	417
NdeI	CATATG	1 cut(s)	622
NdeII	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
NgoMIV	GCCGGC	1 cut(s)	1123
NlaIII	CATG	7 cut(s)	379, 421, 784, 947, 1002, 1318, 1433
NlaIV	GGNNCC	7 cut(s)	52, 78, 94, 109, 211, 458, 604
NmeAIII	GCCGAG	1 cut(s)	226
NmuCI	GTSAC	2 cut(s)	61, 301
NsiI	ATGCAT	2 cut(s)	286, 945
NspI	RCATGY	1 cut(s)	1002
PaeR7I	CTCGAG	1 cut(s)	1434
PagI	TCATGA	1 cut(s)	375
PcsI	WCGNNNNNNNCGW	2 cut(s)	1255, 1264
PdiI	GCCGGC	1 cut(s)	1125
PfeI	GAWTC	4 cut(s)	385, 793, 986, 1103
PleI	GAGTC	4 cut(s)	401, 839, 856, 1263
PpsI	GAGTC	4 cut(s)	401, 839, 856, 1263
PsiI	TTATAA	1 cut(s)	773
Psp6I	CCWGG	1 cut(s)	441
PspGI	CCWGG	1 cut(s)	441
PspN4I	GGNNCC	7 cut(s)	52, 78, 94, 109, 211, 458, 604
PspPI	GGNCC	4 cut(s)	27, 50, 76, 210
PspXI	VCTCGAGB	1 cut(s)	1434
PstI	CTGCAG	2 cut(s)	577, 1017
PstNI	CAGNNNCTG	1 cut(s)	722
PsuI	RGATCY	1 cut(s)	718
PvuII	CAGCTG	1 cut(s)	548
RseI	CAYNNNNRTG	1 cut(s)	1085
SaqAI	TTAA	5 cut(s)	399, 762, 824, 1065, 1492
Sau3AI	GATC	6 cut(s)	372, 438, 718, 1246, 1345, 1408
Sau96I	GGNCC	4 cut(s)	27, 50, 76, 210
SchI	GAGTC	4 cut(s)	401, 840, 856, 1263
ScrFI	CCNGG	1 cut(s)	443
SfcI	CTRYAG	3 cut(s)	428, 573, 1013
Sfr274I	CTCGAG	1 cut(s)	1434
SinI	GGWCC	3 cut(s)	27, 76, 210
SlaI	CTCGAG	1 cut(s)	1434
SmiMI	CAYNNNNRTG	1 cut(s)	1085
SmlI	CTYRAG	1 cut(s)	1434
SmoI	CTYRAG	1 cut(s)	1434
SsiI	CCGC	6 cut(s)	48, 382, 734, 748, 1028, 1178
SspMI	CTAG	1 cut(s)	1485
StyD4I	CCNGG	1 cut(s)	441
StyI	CCWWGG	1 cut(s)	417
TaaI	ACNGT	4 cut(s)	61, 166, 217, 1136
TaqI	TCGA	5 cut(s)	185, 1249, 1258, 1267, 1435
TaqII	GACCGA	1 cut(s)	64
TauI	GCSGC	4 cut(s)	51, 737, 1031, 1181
TfiI	GAWTC	4 cut(s)	385, 793, 986, 1103
Tru1I	TTAA	5 cut(s)	399, 762, 824, 1065, 1492
Tru9I	TTAA	5 cut(s)	399, 762, 824, 1065, 1492
TscAI	CASTG	5 cut(s)	171, 220, 602, 936, 1446
TseFI	GTSAC	2 cut(s)	61, 301
TseI	GCWGC	6 cut(s)	6, 242, 545, 569, 572, 995
Tsp45I	GTSAC	2 cut(s)	61, 301
TspDTI	ATGAA	7 cut(s)	654, 960, 999, 1215, 1380, 1389, 1457
TspGWI	ACGGA	1 cut(s)	105
TspRI	CASTG	5 cut(s)	171, 220, 602, 936, 1446
VpaK11BI	GGWCC	3 cut(s)	27, 76, 210
XapI	RAATTY	3 cut(s)	449, 1222, 1288
XbaI	TCTAGA	1 cut(s)	1484
XceI	RCATGY	1 cut(s)	1002
XhoI	CTCGAG	1 cut(s)	1434
XspI	CTAG	1 cut(s)	1485
Zsp2I	ATGCAT	2 cut(s)	286, 945

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.