Rh2BG561700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005195
Physical Location & Seq
Reverse (-)
77249935 .. 77252143
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG561700.1

Sequence Viewer

Length: 1710 bp
ATGAACGGCACTACCAACATCAAACTCCTGAGGAGATCACTTTGCAGCACCACTGCCCATTTCAAACAACAACCTCTCATCCCACCTTTCACTTCTCTGCAATGTGGAGCCATTCTTCAGTCTCTCACCAACACCAAGTCTCTCCCCAAAGGCCAGAAACTCCACGCCCTCATGCTCACTTGTGGGAATCTTCTACGTAACACCTATCTCAGCTCCAAGCTCGCTTCTTTTTACGCCAGCTGCGGTAATATGGGCCAAGCCCACCTGGTTTTTGACGGCGTTGTGGTTAAAAATTCCTTCTTGTGGAATTTCATGATTAGGGGTTACGCTTGCAATGGGTTTGCGTTGGATTCGATTGTAATGTACCGTGAAATGGTTGGTCTTGGGATAAAGGGGGATAATTTCACGTACCCTTTTGTTCTTAAAGCGTGCGGTGATGTGTTGGCTGTGGAAATTGGGAGGAGGGTTCATGCTGAGGTGGTGGTTAGTGGGTTTGAGTCGAATGTTTATGTGGGTAATGCTCTTGTTGCAATGTACTCAAAGTTTGGGGATATGGGATATGCGTATGAGGTGTTCGATAGAATGCCTGAGAGAGATTTGACATCCTGGAACACTATGGTTTCGGGTTATGTGAAGAATGGTAATCCGAGAGAGGCTTTAGCAGTGTTTGAGAAAATGGGGAAGTTTGGATTAAGGACTGATGGGACAACTTTGCTGGGAATTGTTTCGGCTTGTGGTGAGTTGACGGCATTGAAGCAGGGGAAGGCAATTCATGCGTGTGTGGTGAGGAAAAGTGGTCAAGTATGGAATGAGTTTTTGACAAATTCTCTTATTGAAATGTATTGTAAGTGCAAGTGTGTGGCTTATTCGAGGCGGTTATTTGATGGGGAGGAAGGGAAAGATACTGTGTCATGGAATTCTATGATTAGAGGTTATGAGAGGAATGGTGATGCATTTGAAAGTTTAAGGCTTTTCTGTCGAATGGTTATGGAAGGTGCAGAGGTTGATGAAGTAACAATTGTGACTATGCTTACTTGTTGTGACCAGATCAGCGCCTTGCAATTTGGCATGTCTGTTCATTCATACCTTGTTAAGAAAGGGTTTGGTGCAAATGTCATAGTGGGAACTGCTCTTATAGACATGTATTCTAAATGTGGAAGTTTGTCTTGTTCGCGTCGTTTTTTTGATGACATGCCTAGAAAAAACTTGGTTTCTTGGAGTGCTATGATTTCAGGATATGGAGCTCATGGGAGGGGAGAAGAGTCCATCTCCTGTTATCATGAACTCATAGCAAACAAGTTTAGTCCTGATGAGGGTGTCCTTACTTCTGTTTTGTCAGCATGTAGCCATGCAGGCCTAGTCAATGAGGGTAAAGATATGTTTAATAGAATGACCCGAGAATACAAAGTGAAGCCAACGGTTGCTCACTATTCATGTCTGGTGGATCTTCTCGGCAGAGCAGGGCGCTTAGATGAAGCATATGAACTCATTAAGACTATTGAAGTAGAACCCAGCAGTGATATATGGGCTGCACTTCTCTCTGCTTGCAGGCTATATCCAAATGTCAAGCTGGCAGAGATTTCAGCACAGAAGATTTTCGAAATGAACCCAGAGGGAGTGGGTAGTTACATTTGTCTTTCCAATATCTATGCCGCTGAGAAGCGATGGGATGATGTGGAAAGAGTGAGAGCCATGAGAGTAGTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

569

Amino Acids

63.08

Weight (kDa)

8.36

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 199 - 245 1.5e-07 PPR repeat family
PPR PF01535 202 - 231 2.6e-09 PPR repeat
PPR_2 PF13041 300 - 347 1.2e-08 PPR repeat family
PPR PF01535 303 - 332 2.9e-07 PPR repeat
PPR_1 PF12854 470 - 498 9.1e-06 PPR repeat
E_motif PF20431 519 - 564 2.3e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1174
AciI CCGC 4 cut(s) 243, 432, 874, 1653
AclWI GGATC 1 cut(s) 1452
AcsI RAATTY 4 cut(s) 292, 307, 823, 916
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 3 cut(s) 365, 410, 536
AfiI CCNNNNNNNGG 3 cut(s) 303, 373, 1313
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 5 cut(s) 64, 754, 836, 959, 1502
AjnI CCWGG 2 cut(s) 264, 605
AluBI AGCT 5 cut(s) 213, 220, 240, 1244, 1570
AluI AGCT 5 cut(s) 213, 220, 240, 1244, 1570
Alw21I GWGCWC 1 cut(s) 1246
Alw26I GTCTC 2 cut(s) 126, 144
AlwI GGATC 1 cut(s) 1452
Ama87I CYCGRG 1 cut(s) 1395
AoxI GGCC 3 cut(s) 151, 253, 1354
ApeKI GCWGC 3 cut(s) 45, 240, 1529
ApoI RAATTY 4 cut(s) 292, 307, 823, 916
Asp700I GAANNNNTTC 2 cut(s) 724, 1595
AspLEI GCGC 2 cut(s) 1055, 1467
AspS9I GGNCC 1 cut(s) 253
AsuHPI GGTGA 5 cut(s) 118, 446, 749, 796, 959
AsuII TTCGAA 1 cut(s) 1599
AvaI CYCGRG 1 cut(s) 1395
AxyI CCTNAGG 1 cut(s) 29
BanII GRGCYC 1 cut(s) 1246
Bbv12I GWGCWC 1 cut(s) 1246
BbvCI CCTCAGC 1 cut(s) 474
BbvI GCAGC 3 cut(s) 57, 227, 1516
BccI CCATC 4 cut(s) 695, 878, 1274, 1659
BceAI ACGGC 3 cut(s) 22, 292, 762
BciT130I CCWGG 2 cut(s) 266, 607
BcoDI GTCTC 2 cut(s) 126, 144
BfaI CTAG 2 cut(s) 1197, 1358
BfoI RGCGCY 2 cut(s) 1056, 1468
BglI GCCNNNNNGGC 1 cut(s) 1353
BisI GCNGC 4 cut(s) 46, 241, 1530, 1653
BlsI GCNGC 4 cut(s) 47, 242, 1531, 1654
Bme1390I CCNGG 2 cut(s) 266, 607
BmeT110I CYCGRG 1 cut(s) 1395
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 2 cut(s) 266, 607
BmsI GCATC 1 cut(s) 940
BplI GAGNNNNNCTC 2 cut(s) 1253, 1285
Bpu10I CCTNAGC 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 1599
BsaAI YACGTR 2 cut(s) 197, 408
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 373, 1313
Bse21I CCTNAGG 1 cut(s) 29
Bse3DI GCAATG 3 cut(s) 107, 340, 537
BseBI CCWGG 2 cut(s) 266, 607
BseGI GGATG 3 cut(s) 78, 602, 1675
BseLI CCNNNNNNNGG 3 cut(s) 303, 373, 1313
BseMI GCAATG 3 cut(s) 107, 340, 537
BseMII CTCAG 5 cut(s) 20, 223, 465, 579, 1647
BseRI GAGGAG 2 cut(s) 46, 475
BseXI GCAGC 3 cut(s) 57, 227, 1516
BseYI CCCAGC 2 cut(s) 715, 1511
BsgI GTGCAG 2 cut(s) 1017, 1515
Bsh1236I CGCG 1 cut(s) 1174
BshFI GGCC 3 cut(s) 153, 255, 1356
BsiHKAI GWGCWC 1 cut(s) 1246
BsiHKCI CYCGRG 1 cut(s) 1395
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 3 cut(s) 303, 373, 1313
BsmAI GTCTC 2 cut(s) 126, 144
BsmFI GGGAC 1 cut(s) 718
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 3 cut(s) 153, 255, 1356
BsoBI CYCGRG 1 cut(s) 1395
Bsp119I TTCGAA 1 cut(s) 1599
Bsp1286I GDGCHC 1 cut(s) 1246
Bsp143I GATC 3 cut(s) 35, 1047, 1444
BspACI CCGC 4 cut(s) 243, 432, 874, 1653
BspANI GGCC 3 cut(s) 153, 255, 1356
BspCNI CTCAG 5 cut(s) 21, 222, 466, 580, 1648
BspFNI CGCG 1 cut(s) 1174
BspHI TCATGA 2 cut(s) 312, 1279
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 1 cut(s) 1452
BspT104I TTCGAA 1 cut(s) 1599
BsrDI GCAATG 3 cut(s) 107, 340, 537
BssMI GATC 3 cut(s) 35, 1047, 1444
Bst2UI CCWGG 2 cut(s) 266, 607
Bst4CI ACNGT 3 cut(s) 368, 907, 1420
Bst6I CTCTTC 1 cut(s) 1254
BstAPI GCANNNNNTGC 1 cut(s) 773
BstBAI YACGTR 2 cut(s) 197, 408
BstBI TTCGAA 1 cut(s) 1599
BstC8I GCNNGC 8 cut(s) 222, 238, 331, 430, 1354, 1546, 1550, 1572
BstDEI CTNAG 6 cut(s) 29, 209, 474, 588, 1468, 1656
BstF5I GGATG 3 cut(s) 78, 602, 1675
BstFNI CGCG 1 cut(s) 1174
BstH2I RGCGCY 2 cut(s) 1056, 1468
BstHHI GCGC 2 cut(s) 1055, 1467
BstKTI GATC 3 cut(s) 38, 1050, 1447
BstMAI GTCTC 2 cut(s) 126, 144
BstMBI GATC 3 cut(s) 35, 1047, 1444
BstMWI GCNNNNNNNGC 3 cut(s) 527, 773, 1353
BstNI CCWGG 2 cut(s) 266, 607
BstNSI RCATGY 4 cut(s) 1072, 1144, 1195, 1344
BstSCI CCNGG 2 cut(s) 264, 605
BstSNI TACGTA 1 cut(s) 197
BstUI CGCG 1 cut(s) 1174
BstV1I GCAGC 3 cut(s) 57, 227, 1516
BstX2I RGATCY 1 cut(s) 1444
BstYI RGATCY 1 cut(s) 1444
Bsu36I CCTNAGG 1 cut(s) 29
BsuRI GGCC 3 cut(s) 153, 255, 1356
BtgZI GCGATG 1 cut(s) 1678
BtsCI GGATG 3 cut(s) 78, 602, 1675
BtsI GCAGTG 3 cut(s) 51, 669, 1522
BtsIMutI CAGTG 3 cut(s) 51, 669, 1522
Cac8I GCNNGC 8 cut(s) 222, 238, 331, 430, 1354, 1546, 1550, 1572
CciI TCATGA 2 cut(s) 312, 1279
CfoI GCGC 2 cut(s) 1055, 1467
Cfr13I GGNCC 1 cut(s) 253
CseI GACGC 1 cut(s) 1163
CsiI ACCWGGT 1 cut(s) 264
Csp6I GTAC 3 cut(s) 364, 409, 535
CviQI GTAC 3 cut(s) 364, 409, 535
DdeI CTNAG 6 cut(s) 29, 209, 474, 588, 1468, 1656
DpnI GATC 3 cut(s) 37, 1049, 1446
DpnII GATC 3 cut(s) 35, 1047, 1444
Eam1104I CTCTTC 1 cut(s) 1254
EarI CTCTTC 1 cut(s) 1254
Ecl136II GAGCTC 1 cut(s) 1244
Eco105I TACGTA 1 cut(s) 197
Eco147I AGGCCT 1 cut(s) 1356
Eco24I GRGCYC 1 cut(s) 1246
Eco53kI GAGCTC 1 cut(s) 1244
Eco57I CTGAAG 1 cut(s) 101
Eco81I CCTNAGG 1 cut(s) 29
Eco88I CYCGRG 1 cut(s) 1395
EcoICRI GAGCTC 1 cut(s) 1244
EcoRI GAATTC 1 cut(s) 916
EcoRII CCWGG 2 cut(s) 264, 605
EcoT22I ATGCAT 1 cut(s) 955
EcoT38I GRGCYC 1 cut(s) 1246
FalI AAGNNNNNCTT 2 cut(s) 1150, 1182
FaqI GGGAC 1 cut(s) 718
FauNDI CATATG 1 cut(s) 1480
Fnu4HI GCNGC 4 cut(s) 46, 241, 1530, 1653
FokI GGATG 3 cut(s) 65, 589, 1682
FriOI GRGCYC 1 cut(s) 1246
Fsp4HI GCNGC 4 cut(s) 46, 241, 1530, 1653
FspBI CTAG 2 cut(s) 1197, 1358
GlaI GCGC 2 cut(s) 1054, 1466
GluI GCNGC 4 cut(s) 46, 241, 1530, 1653
GsaI CCCAGC 2 cut(s) 719, 1515
HaeII RGCGCY 2 cut(s) 1056, 1468
HaeIII GGCC 3 cut(s) 153, 255, 1356
HgaI GACGC 1 cut(s) 1163
HhaI GCGC 2 cut(s) 1055, 1467
Hin6I GCGC 2 cut(s) 1053, 1465
HinP1I GCGC 2 cut(s) 1053, 1465
HincII GTYRAC 1 cut(s) 744
HindII GTYRAC 1 cut(s) 744
HinfI GANTC 4 cut(s) 187, 350, 497, 1262
HphI GGTGA 5 cut(s) 118, 446, 749, 796, 959
Hpy166II GTNNAC 1 cut(s) 744
Hpy188I TCNGA 2 cut(s) 648, 1709
Hpy188III TCNNGA 5 cut(s) 28, 313, 1233, 1280, 1307
Hpy8I GTNNAC 1 cut(s) 744
Hpy99I CGWCG 1 cut(s) 1179
HpyAV CCTTC 4 cut(s) 307, 757, 887, 986
HpyCH4III ACNGT 3 cut(s) 368, 907, 1420
HpyCH4IV ACGT 2 cut(s) 196, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 527, 773, 1353
HpyF3I CTNAG 6 cut(s) 29, 209, 474, 588, 1468, 1656
HpySE526I ACGT 2 cut(s) 196, 407
HspAI GCGC 2 cut(s) 1053, 1465
Kzo9I GATC 3 cut(s) 35, 1047, 1444
LmnI GCTCC 3 cut(s) 107, 218, 1241
Lsp1109I GCAGC 3 cut(s) 57, 227, 1516
LweI GCATC 1 cut(s) 940
MabI ACCWGGT 1 cut(s) 264
MaeI CTAG 2 cut(s) 1197, 1358
MaeII ACGT 2 cut(s) 196, 407
MaeIII GTNAC 6 cut(s) 197, 323, 1012, 1021, 1040, 1625
MalI GATC 3 cut(s) 37, 1049, 1446
MboI GATC 3 cut(s) 35, 1047, 1444
MboII GAAGA 6 cut(s) 107, 182, 646, 1271, 1439, 1603
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 1 cut(s) 1444
MhlI GDGCHC 1 cut(s) 1246
MlyI GAGTC 2 cut(s) 506, 1271
MmeI TCCRAC 1 cut(s) 327
Mph1103I ATGCAT 1 cut(s) 955
MroXI GAANNNNTTC 2 cut(s) 724, 1595
MseI TTAA 7 cut(s) 288, 423, 692, 965, 1092, 1383, 1491
MslI CAYNNNNRTG 1 cut(s) 777
MspA1I CMGCKG 2 cut(s) 240, 1655
MspR9I CCNGG 2 cut(s) 266, 607
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 2 cut(s) 266, 607
MvnI CGCG 1 cut(s) 1174
MwoI GCNNNNNNNGC 3 cut(s) 527, 773, 1353
NdeI CATATG 1 cut(s) 1480
NdeII GATC 3 cut(s) 35, 1047, 1444
NlaIV GGNNCC 1 cut(s) 109
NmeAIII GCCGAG 1 cut(s) 1431
NmuCI GTSAC 2 cut(s) 1021, 1040
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 4 cut(s) 1072, 1144, 1195, 1344
NspV TTCGAA 1 cut(s) 1599
PagI TCATGA 2 cut(s) 312, 1279
PceI AGGCCT 1 cut(s) 1356
PciI ACATGT 1 cut(s) 1140
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 2 cut(s) 724, 1595
PfeI GAWTC 2 cut(s) 187, 350
PfoI TCCNGGA 1 cut(s) 605
PkrI GCNGC 4 cut(s) 47, 242, 1531, 1654
PleI GAGTC 2 cut(s) 505, 1270
PpsI GAGTC 2 cut(s) 505, 1270
Ppu21I YACGTR 2 cut(s) 197, 408
PscI ACATGT 1 cut(s) 1140
Psp124BI GAGCTC 1 cut(s) 1246
Psp6I CCWGG 2 cut(s) 264, 605
PspFI CCCAGC 2 cut(s) 715, 1511
PspGI CCWGG 2 cut(s) 264, 605
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 253
PsrI GAACNNNNNNTAC 2 cut(s) 557, 589
PsuI RGATCY 1 cut(s) 1444
PvuII CAGCTG 1 cut(s) 240
RsaI GTAC 3 cut(s) 365, 410, 536
RsaNI GTAC 3 cut(s) 364, 409, 535
RseI CAYNNNNRTG 1 cut(s) 777
SacI GAGCTC 1 cut(s) 1246
SaqAI TTAA 7 cut(s) 288, 423, 692, 965, 1092, 1383, 1491
SatI GCNGC 4 cut(s) 46, 241, 1530, 1653
Sau3AI GATC 3 cut(s) 35, 1047, 1444
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 2 cut(s) 506, 1271
ScrFI CCNGG 2 cut(s) 266, 607
SduI GDGCHC 1 cut(s) 1246
SexAI ACCWGGT 1 cut(s) 264
SfaNI GCATC 1 cut(s) 940
SfuI TTCGAA 1 cut(s) 1599
SmiMI CAYNNNNRTG 1 cut(s) 777
SnaBI TACGTA 1 cut(s) 197
SseBI AGGCCT 1 cut(s) 1356
SsiI CCGC 4 cut(s) 243, 432, 874, 1653
SspMI CTAG 2 cut(s) 1197, 1358
SstI GAGCTC 1 cut(s) 1246
StuI AGGCCT 1 cut(s) 1356
StyD4I CCNGG 2 cut(s) 264, 605
TaaI ACNGT 3 cut(s) 368, 907, 1420
TaiI ACGT 2 cut(s) 199, 410
TaqI TCGA 6 cut(s) 353, 500, 576, 869, 979, 1599
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 1655
TfiI GAWTC 2 cut(s) 187, 350
Tru1I TTAA 7 cut(s) 288, 423, 692, 965, 1092, 1383, 1491
Tru9I TTAA 7 cut(s) 288, 423, 692, 965, 1092, 1383, 1491
TscAI CASTG 3 cut(s) 58, 669, 1522
TseFI GTSAC 2 cut(s) 1021, 1040
TseI GCWGC 3 cut(s) 45, 240, 1529
Tsp45I GTSAC 2 cut(s) 1021, 1040
TspRI CASTG 3 cut(s) 58, 669, 1522
XapI RAATTY 4 cut(s) 292, 307, 823, 916
XceI RCATGY 4 cut(s) 1072, 1144, 1195, 1344
XmnI GAANNNNTTC 2 cut(s) 724, 1595
XspI CTAG 2 cut(s) 1197, 1358
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.