Rh2DG050900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007913
Physical Location & Seq
Forward (+)
3643468 .. 3646641
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG050900.1

Sequence Viewer

Length: 2547 bp
ATGCAACACCAAAATATGCCTCAGGACGGTCTAAAATCAGCTTTGTACAGATCATTCATCACATGTGACGATCCTAAAGGAGTTGTGGATTGTGGGATGATAATGAGGAGATCCAAAAGCAGCGGTGGTTCACAGATTATGGATAAGCAGAAGAAGATCGAAGTCCAAAGAAATTCGAAGAACTCAGTTAGGACATCACCATCTTCATTGGGGTTGATCTCCCAAGAGGAGTTTCAGAGCTCTTCATCATCTTCTTCTTTTCAGCTCATGGAAGTTTCTAGAGGAGCTCAGAGGCTGAACCACATGATTGGTTCCTGGTCGAATGAGAAGAAGAGATTTGATCATGGCCAGTCTAAAGACATTGCAAAAGATTTGCTAAAAGGAGCGCTTGATTTGCAGGAGAGTCTTGTTATGCTTGGGAAGTTGCAAGAAGCTTCACAGTACATGAGTAACTTGAACAAAAAGCACATGGGACAGCCGGAAAGAGCTTACTCGAATCGATATGGAGATCAAGGTTATGTGAAGCCAAGGCTTTCTTCTGATGGATACTCTTCAAGGAATAGTAATGAGGAGCTTAAGAAGGTGATCAGAGACAGCCTTGTGAGGCAAAAATTGTTCGAAAATACAAGCACTGCTGAGAAGGGTTGTGCTTATTCTCCTCAAAGATATAGGGATTCATATTCAGACAGTACTCCTTCCACAAGCTCAAGCCAGTCTTCTATGTATCATAATTCTAGTGAGTATTCCAGCATTGTATCACAAAAGGCGAAAGGTCCAAGTCTGATTGCCAAACTCATGGGAATAGAAGAGTACCCTTTAAAATCTGTGCAGGAGGGTGAGAAGGTTTCAAAACAGCAGAGGCCTGCATTTGAAATTGATAGGCCAAAGGTGAGGAAGCCTCTGATTGCATCTCAAAGTGTTGATCCAGAAAAGAGGACCTTAAAGGAAGTTCTTGACACGATGCGGTTTAAAGGGCTTCTGAGAAACAACTCTTTTGAAGAGCCCAAGTTTGAGATTCATCATTCCAATCAATTGGAATTGGAGAAAAGGTTCAATGGTGATAGTCCACCAATTGTGCTGATCAAGCCTATTTCTGTTGAATCCCCAGAAATGGAAGCAAATGCACCAATTGTTCGGGGAGAGGAAGCTTTCTACAACAGAAGGATGCTTAAGAAATTGAGATCAGAAGAAGAGGTTTCTCCCAAAACAGCGAGTGGTAAAGAGGGAGCTTTGAAGTCTGACAAAGTGCAGCAAAAACTTGAAGTAGAAGAGACATCAATTAGAAAGACTAGCAAAGAAGAAGGAGCTAAAAAGCATCGAGGGGCAGTGGACAAACATGGAGAAAAAGAAGCCAAGACGAAAGAAATGGCTTCGAGAAAGTTGAAAGCTTCTCAACCTGTAGTTCACAAGTCACAGAAGGAGGCAATTGACAAGAGGGTTGATAAGATCCAAAAGGTAGCTGCTGTTAACAGGAACTCAGCGGAGAAGGATAGAGTGATAGCTAAAACCGTGTCAAAGTCTCAAGATCAAGACATTGTGACATCTGCCAAGGCAAGAAAGCCTGAATCTGGATCAAACATCAAAAGGACTCCAGTTTCTCGACAACCAAGTATTGTGACAAGCACAAGTTCAAAACGTTTAACAGTACAGAATGCAGAGTGTACTTCCAGCGAACGCAGAAGGAATCATTTGAGGAAGGAAAAGCCAGTCAAGGAGCCTACAGGAGCTAAATCAGTTGCCAAAAATGTTGTGAGCGAAGAAATTGTCAGGAGAATTGATACCAGTGATAAAAGTGAGTCTTCTCTGATCAGCTCTGAAAATACGCCTATACTAACAAGCTCCAATACTGATTCTAGAGATCAACTCCTTAAAGAAGAGGAAAAGGAAACCTATGGATCCCAGAGTGAAGGGCACTGCAATAGTTTCCAAAGTTCTCTTTCTGAAGCTACATCGCGGAGCTCCATGCAAGAAACAAACCCTGAAACTGCAGTAGAAGTTTCTGGAGACATCAGTCAGAGTGGAAAGGATGGCAAAACAGTCAGAAGTGGTGAAAATCTCAGAGAATTTCTTTTGAGTAATGCATCATTTCTGAGTCATGCCGAAGAGTATTTTGATCTTGATGTGAATGGCTCTACAATCTTACAAACATCTTCTGTCTCCAATTTCGGAGTGTCGAATTCAAGACTAGTTTTAGACTGTGCAAGTGAACTAGTCGAGTGCAAAATATTTCAAGCTTCACAAACAGTTGATCCTCTGTCACTAACATTCAAAGGCAAGTCAAAAGTTTCCATATCGTTGGACAAGTTGGTGGAAGATATTTGGAATGAGATCGAGAATCTGAGAAGTTACAGCAAGCTTGCTGGTGATAACCTTCTTGCAGATAGTCTGTATGCAATGTTGGAGAGAGATGTAATGTGCAAAGGAGTAGGAAAAGGAACATGGGATTTGGGTTGGAGAAATGGATTTTCCGGGGATGAAGGTGAGCAAGTAGTGAGTGACATAGAGAGACTGATTTTCAATGGACTGATTGAGGAGGTCTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

848

Amino Acids

94.55

Weight (kDa)

8.96

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 723 - 845 1.3e-08 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1954
AciI CCGC 4 cut(s) 123, 964, 1481, 1954
AclI AACGTT 1 cut(s) 1636
AclWI GGATC 8 cut(s) 65, 105, 917, 1441, 1579, 1890, 1903, 2243
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 3 cut(s) 172, 2063, 2176
AcuI CTGAAG 1 cut(s) 1962
AfaI GTAC 6 cut(s) 47, 443, 691, 812, 1647, 1663
AfeI AGCGCT 1 cut(s) 387
AfiI CCNNNNNNNGG 3 cut(s) 26, 1111, 1568
AflII CTTAAG 2 cut(s) 575, 1169
AflIII ACRYGT 1 cut(s) 62
AhlI ACTAGT 2 cut(s) 2185, 2209
AjnI CCWGG 1 cut(s) 314
AjuI GAANNNNNNNTTGG 4 cut(s) 216, 248, 520, 552
AloI GAACNNNNNNTCC 2 cut(s) 1034, 1066
Alw21I GWGCWC 3 cut(s) 242, 289, 1961
Alw26I GTCTC 6 cut(s) 585, 1265, 1524, 1998, 2161, 2500
AlwI GGATC 8 cut(s) 65, 105, 917, 1441, 1579, 1890, 1903, 2243
AlwNI CAGNNNCTG 1 cut(s) 295
Aor51HI AGCGCT 1 cut(s) 387
AoxI GGCC 3 cut(s) 346, 860, 881
ApeKI GCWGC 3 cut(s) 120, 1249, 1460
ApoI RAATTY 3 cut(s) 172, 2063, 2176
ArsI GACNNNNNNTTYG 2 cut(s) 1749, 1781
Asp700I GAANNNNTTC 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 2 cut(s) 773, 936
AsuC2I CCSGG 1 cut(s) 2470
AsuHPI GGTGA 8 cut(s) 189, 595, 848, 901, 1070, 2060, 2375, 2492
AsuII TTCGAA 2 cut(s) 176, 618
AvaII GGWCC 2 cut(s) 773, 936
AxyI CCTNAGG 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 1914
BalI TGGCCA 1 cut(s) 348
BamHI GGATCC 1 cut(s) 1895
BanII GRGCYC 4 cut(s) 242, 289, 1005, 1961
BbsI GAAGAC 2 cut(s) 708, 1791
Bbv12I GWGCWC 3 cut(s) 242, 289, 1961
BbvI GCAGC 3 cut(s) 132, 1261, 1447
BccI CCATC 3 cut(s) 208, 536, 2021
BciT130I CCWGG 1 cut(s) 316
BciVI GTATCC 1 cut(s) 539
BclI TGATCA 4 cut(s) 340, 585, 1080, 1806
BcnI CCSGG 1 cut(s) 2470
BcoDI GTCTC 6 cut(s) 585, 1265, 1524, 1998, 2161, 2500
BcuI ACTAGT 2 cut(s) 2185, 2209
BfaI CTAG 6 cut(s) 279, 735, 1290, 1854, 2186, 2210
BfmI CTRYAG 3 cut(s) 1398, 1719, 1986
BfoI RGCGCY 1 cut(s) 389
BfrI CTTAAG 2 cut(s) 575, 1169
BfuI GTATCC 1 cut(s) 539
BisI GCNGC 3 cut(s) 121, 1250, 1461
BlsI GCNGC 3 cut(s) 122, 1251, 1462
BmcAI AGTACT 1 cut(s) 691
Bme1390I CCNGG 2 cut(s) 316, 2470
Bme18I GGWCC 2 cut(s) 773, 936
BmgT120I GGNCC 2 cut(s) 773, 936
BmiI GGNNCC 3 cut(s) 313, 1716, 1897
BmrFI CCNGG 2 cut(s) 316, 2470
BmsI GCATC 5 cut(s) 917, 951, 1155, 1324, 2090
BoxI GACNNNNGTC 1 cut(s) 2010
BpiI GAAGAC 2 cut(s) 708, 1791
BplI GAGNNNNNCTC 4 cut(s) 883, 915, 1848, 1880
BpmI CTGGAG 2 cut(s) 1575, 2022
Bpu14I TTCGAA 2 cut(s) 176, 618
BpuEI CTTGAG 2 cut(s) 691, 1506
BpuMI CCSGG 1 cut(s) 2470
Bsa29I ATCGAT 1 cut(s) 499
BsaJI CCNNGG 3 cut(s) 527, 1548, 2469
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 1111, 1568
Bse1I ACTGG 5 cut(s) 349, 712, 1592, 1706, 1782
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 2 cut(s) 360, 2400
BseBI CCWGG 1 cut(s) 316
BseCI ATCGAT 1 cut(s) 499
BseDI CCNNGG 3 cut(s) 527, 1548, 2469
BseGI GGATG 4 cut(s) 102, 1170, 2032, 2479
BseLI CCNNNNNNNGG 3 cut(s) 26, 1111, 1568
BseMI GCAATG 2 cut(s) 360, 2400
BseMII CTCAG 9 cut(s) 35, 198, 302, 627, 971, 1491, 2071, 2081, 2330
BseNI ACTGG 5 cut(s) 349, 712, 1592, 1706, 1782
BseRI GAGGAG 6 cut(s) 121, 242, 297, 584, 648, 2546
BseSI GKGCMC 1 cut(s) 1914
BseXI GCAGC 3 cut(s) 132, 1261, 1447
BsgI GTGCAG 2 cut(s) 848, 1268
Bsh1236I CGCG 1 cut(s) 1954
BshFI GGCC 3 cut(s) 348, 862, 883
BshVI ATCGAT 1 cut(s) 499
BsiHKAI GWGCWC 3 cut(s) 242, 289, 1961
BsiSI CCGG 2 cut(s) 479, 2469
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 3 cut(s) 26, 1111, 1568
BsmAI GTCTC 6 cut(s) 585, 1265, 1524, 1998, 2161, 2500
BsmFI GGGAC 1 cut(s) 486
BsmI GAATGC 1 cut(s) 1657
BsnI GGCC 3 cut(s) 348, 862, 883
Bsp119I TTCGAA 2 cut(s) 176, 618
Bsp1286I GDGCHC 5 cut(s) 242, 289, 1005, 1914, 1961
Bsp1407I TGTACA 1 cut(s) 45
BspACI CCGC 4 cut(s) 123, 964, 1481, 1954
BspANI GGCC 3 cut(s) 348, 862, 883
BspCNI CTCAG 9 cut(s) 34, 197, 301, 628, 972, 1490, 2070, 2082, 2331
BspDI ATCGAT 1 cut(s) 499
BspFNI CGCG 1 cut(s) 1954
BspLI GGNNCC 3 cut(s) 313, 1716, 1897
BspMAI CTGCAG 1 cut(s) 1990
BspPI GGATC 8 cut(s) 65, 105, 917, 1441, 1579, 1890, 1903, 2243
BspQI GCTCTTC 2 cut(s) 247, 993
BspT104I TTCGAA 2 cut(s) 176, 618
BspTI CTTAAG 2 cut(s) 575, 1169
BsrDI GCAATG 2 cut(s) 360, 2400
BsrGI TGTACA 1 cut(s) 45
BsrI ACTGG 5 cut(s) 349, 712, 1592, 1706, 1782
BssECI CCNNGG 3 cut(s) 527, 1548, 2469
BssT1I CCWWGG 2 cut(s) 527, 1548
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 8 cut(s) 29, 441, 689, 1510, 1645, 2038, 2198, 2245
Bst6I CTCTTC 9 cut(s) 247, 326, 556, 801, 993, 1185, 1263, 1869, 2097
BstAFI CTTAAG 2 cut(s) 575, 1169
BstAUI TGTACA 1 cut(s) 45
BstBI TTCGAA 2 cut(s) 176, 618
BstC8I GCNNGC 3 cut(s) 864, 2354, 2358
BstDEI CTNAG 9 cut(s) 21, 184, 288, 636, 980, 1477, 2057, 2090, 2339
BstF5I GGATG 4 cut(s) 102, 1170, 2032, 2479
BstFNI CGCG 1 cut(s) 1954
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 1 cut(s) 388
BstMAI GTCTC 6 cut(s) 585, 1265, 1524, 1998, 2161, 2500
BstMWI GCNNNNNNNGC 2 cut(s) 394, 1084
BstNI CCWGG 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 66
BstPAI GACNNNNGTC 1 cut(s) 2010
BstSCI CCNGG 2 cut(s) 314, 2468
BstSFI CTRYAG 3 cut(s) 1398, 1719, 1986
BstSLI GKGCMC 1 cut(s) 1914
BstUI CGCG 1 cut(s) 1954
BstV1I GCAGC 3 cut(s) 132, 1261, 1447
BstV2I GAAGAC 2 cut(s) 708, 1791
BstX2I RGATCY 3 cut(s) 110, 1446, 1895
BstXI CCANNNNNNTGG 4 cut(s) 308, 796, 1033, 2296
BstYI RGATCY 3 cut(s) 110, 1446, 1895
Bsu15I ATCGAT 1 cut(s) 499
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 3 cut(s) 348, 862, 883
BsuTUI ATCGAT 1 cut(s) 499
BtgZI GCGATG 1 cut(s) 1935
BtsCI GGATG 4 cut(s) 102, 1170, 2032, 2479
BtsI GCAGTG 3 cut(s) 630, 1332, 1912
BtsIMutI CAGTG 4 cut(s) 630, 1332, 1789, 1912
Cac8I GCNNGC 3 cut(s) 864, 2354, 2358
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 1 cut(s) 388
Cfr13I GGNCC 2 cut(s) 773, 936
ClaI ATCGAT 1 cut(s) 499
Csp6I GTAC 6 cut(s) 46, 442, 690, 811, 1646, 1662
CviQI GTAC 6 cut(s) 46, 442, 690, 811, 1646, 1662
DdeI CTNAG 9 cut(s) 21, 184, 288, 636, 980, 1477, 2057, 2090, 2339
DraI TTTAAA 2 cut(s) 819, 970
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 9 cut(s) 247, 326, 556, 801, 993, 1185, 1263, 1869, 2097
EarI CTCTTC 9 cut(s) 247, 326, 556, 801, 993, 1185, 1263, 1869, 2097
Ecl136II GAGCTC 3 cut(s) 240, 287, 1959
Eco130I CCWWGG 2 cut(s) 527, 1548
Eco147I AGGCCT 1 cut(s) 862
Eco24I GRGCYC 4 cut(s) 242, 289, 1005, 1961
Eco47I GGWCC 2 cut(s) 773, 936
Eco47III AGCGCT 1 cut(s) 387
Eco53kI GAGCTC 3 cut(s) 240, 287, 1959
Eco57I CTGAAG 1 cut(s) 1962
Eco81I CCTNAGG 1 cut(s) 21
EcoICRI GAGCTC 3 cut(s) 240, 287, 1959
EcoO109I RGGNCCY 1 cut(s) 936
EcoRI GAATTC 1 cut(s) 2176
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 2 cut(s) 527, 1548
EcoT22I ATGCAT 1 cut(s) 2083
EcoT38I GRGCYC 4 cut(s) 242, 289, 1005, 1961
ErhI CCWWGG 2 cut(s) 527, 1548
FaqI GGGAC 1 cut(s) 486
FbaI TGATCA 4 cut(s) 340, 585, 1080, 1806
Fnu4HI GCNGC 3 cut(s) 121, 1250, 1461
FokI GGATG 4 cut(s) 109, 1177, 2039, 2486
FriOI GRGCYC 4 cut(s) 242, 289, 1005, 1961
Fsp4HI GCNGC 3 cut(s) 121, 1250, 1461
FspBI CTAG 6 cut(s) 279, 735, 1290, 1854, 2186, 2210
GlaI GCGC 1 cut(s) 387
GluI GCNGC 3 cut(s) 121, 1250, 1461
GsuI CTGGAG 2 cut(s) 1575, 2022
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 3 cut(s) 348, 862, 883
HapII CCGG 2 cut(s) 479, 2469
HhaI GCGC 1 cut(s) 388
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HincII GTYRAC 1 cut(s) 1468
HindII GTYRAC 1 cut(s) 1468
HindIII AAGCTT 5 cut(s) 432, 1146, 1386, 2232, 2354
HpaI GTTAAC 1 cut(s) 1468
HpaII CCGG 2 cut(s) 479, 2469
HphI GGTGA 8 cut(s) 189, 595, 848, 901, 1070, 2060, 2375, 2492
Hpy166II GTNNAC 7 cut(s) 131, 1067, 1330, 1405, 1468, 1662, 2207
Hpy8I GTNNAC 7 cut(s) 131, 1067, 1330, 1405, 1468, 1662, 2207
HpyCH4III ACNGT 8 cut(s) 29, 441, 689, 1510, 1645, 2038, 2198, 2245
HpyCH4IV ACGT 1 cut(s) 1636
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 1084
HpyF3I CTNAG 9 cut(s) 21, 184, 288, 636, 980, 1477, 2057, 2090, 2339
HpySE526I ACGT 1 cut(s) 1636
HspAI GCGC 1 cut(s) 386
Ksp22I TGATCA 4 cut(s) 340, 585, 1080, 1806
KspAI GTTAAC 1 cut(s) 1468
LguI GCTCTTC 2 cut(s) 247, 993
Lsp1109I GCAGC 3 cut(s) 132, 1261, 1447
LweI GCATC 5 cut(s) 917, 951, 1155, 1324, 2090
MaeI CTAG 6 cut(s) 279, 735, 1290, 1854, 2186, 2210
MaeII ACGT 1 cut(s) 1636
MaeIII GTNAC 8 cut(s) 65, 449, 1410, 1537, 1615, 2256, 2345, 2495
MfeI CAATTG 4 cut(s) 1031, 1071, 1128, 1425
MflI RGATCY 3 cut(s) 110, 1446, 1895
MhlI GDGCHC 5 cut(s) 242, 289, 1005, 1914, 1961
MlsI TGGCCA 1 cut(s) 348
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 4 cut(s) 412, 1582, 1805, 2101
MmeI TCCRAC 3 cut(s) 2277, 2379, 2432
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 1 cut(s) 2083
MroXI GAANNNNTTC 1 cut(s) 1049
MscI TGGCCA 1 cut(s) 348
MseI TTAA 8 cut(s) 576, 818, 941, 969, 1170, 1467, 1640, 1869
Msp20I TGGCCA 1 cut(s) 348
MspA1I CMGCKG 2 cut(s) 123, 1481
MspCI CTTAAG 2 cut(s) 575, 1169
MspI CCGG 2 cut(s) 479, 2469
MspR9I CCNGG 2 cut(s) 316, 2470
MunI CAATTG 4 cut(s) 1031, 1071, 1128, 1425
Mva1269I GAATGC 1 cut(s) 1657
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 1954
MwoI GCNNNNNNNGC 2 cut(s) 394, 1084
NciI CCSGG 1 cut(s) 2470
NlaIV GGNNCC 3 cut(s) 313, 1716, 1897
NmuCI GTSAC 6 cut(s) 65, 1410, 1537, 1615, 2256, 2495
NsiI ATGCAT 1 cut(s) 2083
NspI RCATGY 1 cut(s) 66
NspV TTCGAA 2 cut(s) 176, 618
PceI AGGCCT 1 cut(s) 862
PciI ACATGT 1 cut(s) 62
PciSI GCTCTTC 2 cut(s) 247, 993
PctI GAATGC 1 cut(s) 1657
PdmI GAANNNNTTC 1 cut(s) 1049
PfeI GAWTC 8 cut(s) 496, 674, 1015, 1100, 1565, 1684, 1850, 2335
PkrI GCNGC 3 cut(s) 122, 1251, 1462
PleI GAGTC 4 cut(s) 411, 1582, 1804, 2100
PpsI GAGTC 4 cut(s) 411, 1582, 1804, 2100
PpuMI RGGWCCY 1 cut(s) 936
PscI ACATGT 1 cut(s) 62
PshAI GACNNNNGTC 1 cut(s) 2010
Psp124BI GAGCTC 3 cut(s) 242, 289, 1961
Psp1406I AACGTT 1 cut(s) 1636
Psp5II RGGWCCY 1 cut(s) 936
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 3 cut(s) 313, 1716, 1897
PspPI GGNCC 2 cut(s) 773, 936
PspPPI RGGWCCY 1 cut(s) 936
PstI CTGCAG 1 cut(s) 1990
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 3 cut(s) 110, 1446, 1895
RsaI GTAC 6 cut(s) 47, 443, 691, 812, 1647, 1663
RsaNI GTAC 6 cut(s) 46, 442, 690, 811, 1646, 1662
SacI GAGCTC 3 cut(s) 242, 289, 1961
SapI GCTCTTC 2 cut(s) 247, 993
SaqAI TTAA 8 cut(s) 576, 818, 941, 969, 1170, 1467, 1640, 1869
SatI GCNGC 3 cut(s) 121, 1250, 1461
Sau96I GGNCC 2 cut(s) 773, 936
ScaI AGTACT 1 cut(s) 691
SchI GAGTC 4 cut(s) 412, 1582, 1805, 2101
ScrFI CCNGG 2 cut(s) 316, 2470
SduI GDGCHC 5 cut(s) 242, 289, 1005, 1914, 1961
SfaNI GCATC 5 cut(s) 917, 951, 1155, 1324, 2090
SfcI CTRYAG 3 cut(s) 1398, 1719, 1986
SfuI TTCGAA 2 cut(s) 176, 618
SinI GGWCC 2 cut(s) 773, 936
SmlI CTYRAG 4 cut(s) 575, 706, 1169, 1521
SmoI CTYRAG 4 cut(s) 575, 706, 1169, 1521
SpeI ACTAGT 2 cut(s) 2185, 2209
SseBI AGGCCT 1 cut(s) 862
SsiI CCGC 4 cut(s) 123, 964, 1481, 1954
SspI AATATT 1 cut(s) 2226
SspMI CTAG 6 cut(s) 279, 735, 1290, 1854, 2186, 2210
SstI GAGCTC 3 cut(s) 242, 289, 1961
StuI AGGCCT 1 cut(s) 862
StyD4I CCNGG 2 cut(s) 314, 2468
StyI CCWWGG 2 cut(s) 527, 1548
TaaI ACNGT 8 cut(s) 29, 441, 689, 1510, 1645, 2038, 2198, 2245
TaiI ACGT 1 cut(s) 1639
TatI WGTACW 5 cut(s) 45, 441, 689, 1645, 1661
TfiI GAWTC 8 cut(s) 496, 674, 1015, 1100, 1565, 1684, 1850, 2335
Tru1I TTAA 8 cut(s) 576, 818, 941, 969, 1170, 1467, 1640, 1869
Tru9I TTAA 8 cut(s) 576, 818, 941, 969, 1170, 1467, 1640, 1869
TscAI CASTG 4 cut(s) 637, 1332, 1789, 1919
TseFI GTSAC 6 cut(s) 65, 1410, 1537, 1615, 2256, 2495
TseI GCWGC 3 cut(s) 120, 1249, 1460
Tsp45I GTSAC 6 cut(s) 65, 1410, 1537, 1615, 2256, 2495
TspDTI ATGAA 6 cut(s) 46, 195, 234, 666, 1007, 2490
TspRI CASTG 4 cut(s) 637, 1332, 1789, 1919
Vha464I CTTAAG 2 cut(s) 575, 1169
VpaK11BI GGWCC 2 cut(s) 773, 936
XapI RAATTY 3 cut(s) 172, 2063, 2176
XbaI TCTAGA 2 cut(s) 278, 1853
XceI RCATGY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 1049
XspI CTAG 6 cut(s) 279, 735, 1290, 1854, 2186, 2210
ZrmI AGTACT 1 cut(s) 691
Zsp2I ATGCAT 1 cut(s) 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.