Rh2DG290900

threonine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016243
Physical Location & Seq
Reverse (-)
34160290 .. 34168399
8110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG290900.1

Sequence Viewer

Length: 1113 bp
ATGCAAACGATGGCGTTCCCCCAGAAGACGATGTCGACGACGTTGCCTTCCCCATGGAGATGCTCACTCGACGATCTCGGTGTGGATGGTGTCTTCGGATTCGGCTGCCGTGATCTGCTTTCTGGTTCGCTCCGGGATATGGATTTCAATCTAGTTGGGAACTTACAGCTGTCTAACAAAGAAGTGAGAGAAAGGGAACCTATCGGAGTAAGTGAGCAGGGAAAATTCTTAAAGATGTCAGTGTACAATCTCCCAAGTGTGCAGCTACTGAGTAACTCAAAGAAAACCATCTCTACCCGGAAAGTTTCCAACTGTAGACTTCGTAGACCTTTCATGGTGAATGTCATGGAACTTGAAATTCCTTCAGCAAAGGAAACTGATGGAAATGCTGAAAAGGCTGAAGCATATACCAAGGCGTTTAAAGCATTACCAGCTGCTGCTGCCACAACACAAAAAATCTATGAACTCAATGAGCGTGTTGTGGCAGCAGCAGCTGGTAATGCTGCTATATGTCAGGTTTTGTTACGGACGGTACAAAATGAAGTGGAGATTGAGGATAATATATACACTGTGAAAGCAGCATGTGAGAGGTATATTGATGAGTGGAAGAGAAAGAAGAAACAGGAAAATAAGGATGAACATTTCCCTTCAACTATTGGGCTCTATAGCTGGGAAGGGCAGCAGGCTGTTATAATATTTGTTGGATTCCGCAACCAAATGGCGACAGAGTTGGGAAAAGTCATGGCATGTGGGGCTGGTAGTAGCTGGGCTGAAAACTATCTGGGTTCACAGACGTGGAGAGATGATTTTACCAAGTTTGATTTTGTTCACCTTGTCAGGCAATCAGTCGCACTTTCCAGTTTGCATGGTCCTTCTACGGGTGGTAGGATTACAGTGCTAAGATTGTCTCGTGGAGGTGACAGGGAAATATTTCCCATGCATGATCATGTAATCAAATGCTTGGGTGACTTTTTCTATTTCTTTATTCTGTTTATGGCTCTATGTAACCAAAAAGGAAAAAAAAAATTACTTGGTTTTTTTTTTAAGCCATATCAATTATATCAAAGAGCCAGAATTCGGCAATTCACCAGATCAACAACCCATAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0001775 GO:0002376 GO:0002520 GO:0002521 GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005829 GO:0005839 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010498 GO:0010499 GO:0012505 GO:0016020 GO:0016043 GO:0016579 GO:0016787 GO:0019221 GO:0019222 GO:0019538 GO:0019774 GO:0019882 GO:0019941 GO:0022607 GO:0023052 GO:0030097 GO:0030098 GO:0030154 GO:0030162 GO:0030163 GO:0030217 GO:0031597 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0034097 GO:0034340 GO:0034515 GO:0034622 GO:0036037 GO:0036211 GO:0042110 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043374 GO:0043412 GO:0043632 GO:0043687 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045087 GO:0045321 GO:0045444 GO:0046631 GO:0046632 GO:0046649 GO:0048513 GO:0048534 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051336 GO:0051603 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060337 GO:0065003 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070646 GO:0070647 GO:0070887 GO:0071310 GO:0071345 GO:0071357 GO:0071704 GO:0071840 GO:0080090 GO:0080129 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369 GO:1990111
KEGG Pathways
Metabolic & Signaling
ko02010 ko03050 ko04145 ko04612 ko05168 ko05340 map02010 map03050 map04145 map04612 map05168 map05340
Pfam Domains
Protein Families
Proteasome

Protein Analysis

370

Amino Acids

41.86

Weight (kDa)

9.28

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 147 - 299 3.2e-06 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016243)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22660
pyrus_communis pycom05g28180
rosa_chinensis RchiOBHm_Chr2g0116881
rosa_laevigata RLG00000018303
rosa_multiflora Rmu_sc0002177.1_g000017 Rmu_sc0005387.1_g000003 Rmu_sc0006730.1_g000003
rosa_roxburghii Rroxscaffold_2G00126940 Rroxscaffold_6G00390420
rosa_rugosa Rorug02G0208900
rosa_samantha Rh2AG264500 Rh2BG275700 Rh2DG271800 Rh2DG290900
rosa_wichuraiana Rw2G020810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
AccI GTMKAC 3 cut(s) 35, 316, 325
AciI CCGC 1 cut(s) 709
AcsI RAATTY 3 cut(s) 224, 357, 1074
AcuI CTGAAG 2 cut(s) 348, 420
AfaI GTAC 2 cut(s) 245, 534
AfiI CCNNNNNNNGG 3 cut(s) 139, 878, 1077
AgsI TTSAA 3 cut(s) 148, 356, 651
AjiI CACGTC 1 cut(s) 795
AleI CACNNNNGTG 1 cut(s) 793
AluBI AGCT 6 cut(s) 169, 265, 434, 494, 669, 765
AluI AGCT 6 cut(s) 169, 265, 434, 494, 669, 765
Alw26I GTCTC 1 cut(s) 912
AlwNI CAGNNNCTG 3 cut(s) 268, 437, 494
ApoI RAATTY 3 cut(s) 224, 357, 1074
Asp700I GAANNNNTTC 1 cut(s) 930
AspS9I GGNCC 1 cut(s) 869
AsuC2I CCSGG 2 cut(s) 134, 298
AsuHPI GGTGA 5 cut(s) 349, 821, 929, 977, 1078
AvaII GGWCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 663
BauI CACGAG 1 cut(s) 909
BbsI GAAGAC 2 cut(s) 32, 85
BccI CCATC 4 cut(s) 4, 80, 296, 374
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BclI TGATCA 1 cut(s) 943
BcnI CCSGG 2 cut(s) 134, 298
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 2 cut(s) 313, 664
Bme1390I CCNGG 2 cut(s) 134, 298
Bme18I GGWCC 1 cut(s) 869
BmgBI CACGTC 1 cut(s) 795
BmgT120I GGNCC 1 cut(s) 869
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 2 cut(s) 134, 298
BmsI GCATC 1 cut(s) 50
BpiI GAAGAC 2 cut(s) 32, 85
BpuMI CCSGG 2 cut(s) 134, 298
BsaBI GATNNNNATC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 53, 411
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 878, 1077
Bse1I ACTGG 1 cut(s) 858
Bse8I GATNNNNATC 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 53, 411
BseGI GGATG 2 cut(s) 91, 640
BseJI GATNNNNATC 1 cut(s) 147
BseLI CCNNNNNNNGG 3 cut(s) 139, 878, 1077
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 1 cut(s) 858
BseYI CCCAGC 2 cut(s) 669, 765
BsgI GTGCAG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 133, 298
BslI CCNNNNNNNGG 3 cut(s) 139, 878, 1077
BsmAI GTCTC 1 cut(s) 912
Bsp1286I GDGCHC 1 cut(s) 663
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 4 cut(s) 73, 112, 943, 1091
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 1 cut(s) 709
BspCNI CTCAG 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 198
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 858
BssECI CCNNGG 2 cut(s) 53, 411
BssMI GATC 4 cut(s) 73, 112, 943, 1091
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 2 cut(s) 53, 411
Bst2BI CACGAG 1 cut(s) 909
Bst4CI ACNGT 4 cut(s) 314, 532, 571, 895
Bst6I CTCTTC 1 cut(s) 602
BstAUI TGTACA 1 cut(s) 243
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 2 cut(s) 269, 899
BstDSI CCRYGG 1 cut(s) 53
BstF5I GGATG 2 cut(s) 91, 640
BstKTI GATC 4 cut(s) 76, 115, 946, 1094
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 4 cut(s) 73, 112, 943, 1091
BstMWI GCNNNNNNNGC 7 cut(s) 395, 422, 431, 440, 491, 500, 752
BstNSI RCATGY 2 cut(s) 585, 750
BstSCI CCNGG 2 cut(s) 132, 296
BstSFI CTRYAG 2 cut(s) 313, 664
BstV2I GAAGAC 2 cut(s) 32, 85
BtgI CCRYGG 1 cut(s) 53
BtrI CACGTC 1 cut(s) 795
BtsCI GGATG 2 cut(s) 91, 640
BtsIMutI CAGTG 3 cut(s) 246, 567, 900
Cac8I GCNNGC 1 cut(s) 684
CaiI CAGNNNCTG 3 cut(s) 268, 437, 494
Cfr13I GGNCC 1 cut(s) 869
Csp6I GTAC 2 cut(s) 244, 533
CviQI GTAC 2 cut(s) 244, 533
DdeI CTNAG 2 cut(s) 269, 899
DpnI GATC 4 cut(s) 75, 114, 945, 1093
DpnII GATC 4 cut(s) 73, 112, 943, 1091
DraI TTTAAA 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
Eco130I CCWWGG 2 cut(s) 53, 411
Eco24I GRGCYC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 869
Eco57I CTGAAG 2 cut(s) 348, 420
EcoRI GAATTC 1 cut(s) 1074
EcoT14I CCWWGG 2 cut(s) 53, 411
EcoT22I ATGCAT 1 cut(s) 942
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 2 cut(s) 53, 411
FbaI TGATCA 1 cut(s) 943
FblI GTMKAC 3 cut(s) 35, 316, 325
FokI GGATG 2 cut(s) 98, 647
FriOI GRGCYC 1 cut(s) 663
FspBI CTAG 1 cut(s) 152
GsaI CCCAGC 2 cut(s) 673, 769
HapII CCGG 2 cut(s) 133, 298
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 2 cut(s) 99, 705
HpaII CCGG 2 cut(s) 133, 298
HphI GGTGA 5 cut(s) 349, 821, 929, 977, 1078
Hpy166II GTNNAC 6 cut(s) 36, 244, 317, 326, 788, 829
Hpy188I TCNGA 2 cut(s) 98, 206
Hpy8I GTNNAC 6 cut(s) 36, 244, 317, 326, 788, 829
Hpy99I CGWCG 3 cut(s) 40, 43, 74
HpyAV CCTTC 5 cut(s) 57, 372, 657, 668, 882
HpyCH4III ACNGT 4 cut(s) 314, 532, 571, 895
HpyCH4IV ACGT 2 cut(s) 41, 794
HpyCH4V TGCA 4 cut(s) 4, 262, 865, 940
HpyF10VI GCNNNNNNNGC 7 cut(s) 395, 422, 431, 440, 491, 500, 752
HpyF3I CTNAG 2 cut(s) 269, 899
HpySE526I ACGT 2 cut(s) 41, 794
Ksp22I TGATCA 1 cut(s) 943
Kzo9I GATC 4 cut(s) 73, 112, 943, 1091
LmnI GCTCC 1 cut(s) 135
LweI GCATC 1 cut(s) 50
MaeI CTAG 1 cut(s) 152
MaeII ACGT 2 cut(s) 41, 794
MaeIII GTNAC 5 cut(s) 272, 522, 917, 965, 1004
MalI GATC 4 cut(s) 75, 114, 945, 1093
MboI GATC 4 cut(s) 73, 112, 943, 1091
MboII GAAGA 4 cut(s) 37, 85, 619, 628
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 6 cut(s) 224, 357, 1025, 1055, 1074, 1082
MmeI TCCRAC 2 cut(s) 333, 682
MnlI CCTC 3 cut(s) 547, 582, 908
Mph1103I ATGCAT 1 cut(s) 942
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 3 cut(s) 230, 420, 1044
MslI CAYNNNNRTG 3 cut(s) 58, 793, 945
MspA1I CMGCKG 3 cut(s) 169, 434, 494
MspI CCGG 2 cut(s) 133, 298
MspR9I CCNGG 2 cut(s) 134, 298
MwoI GCNNNNNNNGC 7 cut(s) 395, 422, 431, 440, 491, 500, 752
NciI CCSGG 2 cut(s) 134, 298
NcoI CCATGG 1 cut(s) 53
NdeII GATC 4 cut(s) 73, 112, 943, 1091
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 2 cut(s) 917, 965
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 2 cut(s) 585, 750
OliI CACNNNNGTG 1 cut(s) 793
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 2 cut(s) 99, 705
PflFI GACNNNGTC 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 132
PsiI TTATAA 1 cut(s) 692
PspFI CCCAGC 2 cut(s) 669, 765
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 869
PstNI CAGNNNCTG 3 cut(s) 268, 437, 494
PsyI GACNNNGTC 1 cut(s) 31
PvuII CAGCTG 3 cut(s) 169, 434, 494
RsaI GTAC 2 cut(s) 245, 534
RsaNI GTAC 2 cut(s) 244, 533
RseI CAYNNNNRTG 3 cut(s) 58, 793, 945
SalI GTCGAC 1 cut(s) 34
SaqAI TTAA 3 cut(s) 230, 420, 1044
Sau3AI GATC 4 cut(s) 73, 112, 943, 1091
Sau96I GGNCC 1 cut(s) 869
ScrFI CCNGG 2 cut(s) 134, 298
SduI GDGCHC 1 cut(s) 663
SfaNI GCATC 1 cut(s) 50
SfcI CTRYAG 2 cut(s) 313, 664
SinI GGWCC 1 cut(s) 869
SmiMI CAYNNNNRTG 3 cut(s) 58, 793, 945
Sse9I AATT 6 cut(s) 224, 357, 1025, 1055, 1074, 1082
SsiI CCGC 1 cut(s) 709
SspI AATATT 2 cut(s) 696, 930
SspMI CTAG 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 132, 296
StyI CCWWGG 2 cut(s) 53, 411
TaaI ACNGT 4 cut(s) 314, 532, 571, 895
TaiI ACGT 2 cut(s) 44, 797
TaqI TCGA 2 cut(s) 35, 69
TasI AATT 6 cut(s) 224, 357, 1025, 1055, 1074, 1082
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 2 cut(s) 99, 705
Tru1I TTAA 3 cut(s) 230, 420, 1044
Tru9I TTAA 3 cut(s) 230, 420, 1044
TscAI CASTG 3 cut(s) 246, 574, 900
TseFI GTSAC 2 cut(s) 917, 965
Tsp45I GTSAC 2 cut(s) 917, 965
TspDTI ATGAA 4 cut(s) 322, 477, 555, 651
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 3 cut(s) 246, 574, 900
Tth111I GACNNNGTC 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 3 cut(s) 224, 357, 1074
XceI RCATGY 2 cut(s) 585, 750
XmiI GTMKAC 3 cut(s) 35, 316, 325
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.