Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0007283

Physical Location & Seq

Genomic Coordinates Reverse (-)

87845469 .. 87849481

Gene Structure 4013 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG653400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGTTCACTCTGCAACTTCCGAGTTGCAGAGCTCTCGATCGCTCGCCGCCGCCGCCATTGCTCGGCGGCGGCTCCTCGTTTTGGGGGACGGCGGTCGTCAATTTCAATGGCAGATCCACGGCGGAGAAGAAGCTCCGGTTGGACGGACGGACGGTCAGGATCGTGGCGATGTCGTCGTCGTCGAGCAGCAACAACACTTTCAAGATGAACTTGAACGAGTACATGGTCACTCTCGAGAAGCCGCTCGGGATCCGTTTCGCGCTCTCCGTCGACGGCAAAATCTTCGTCCACGCTCTCAAGAAAGGGGGGAATGCGGAGAAGTCCAGGATAGTAATGGTGGGCGACACTTTGAAAAAGGCGGGTAATTCATCTGAAGGGAGGCTTATTGAGACCAAGGACTTCGGTGATACACAGAAGATGCTGAAGGAGAAGACAGGATCTTATAGTCTTGTTCTTGAGAGGCCATTTTCTCCCTTTCCAATTCAACAGCTGCTTCTTATGAATGATCTTGATAATCTATTCAATAGAGGTCGAGTTCCTTTTGCAACTTGGAACAACCGTGTATTGGCTTCAAATTTGCAATCATCTGCTGGAAGCAGTGGCAATTCTGGTTTTGTCACGTTCTCCTCAAAGTTTCTAAAACCACAAGGATGGAATTCCTTGAATGGAAATGGTCATGTTCAACCAACAGTGCAGAGGAACACTACTGCCAAACCCATAAGCCAACTGGTTTGTATTCTGTCCGAAGAGGAGCCTGGAGATGGAGAATGGGCTTACGGAAATTTTCCACTAGAAGAATATATTAAAGCACTGGATCGCTCAAAAGGCGAATTATATTATGATCACTCACTTGGTATGCGCTATAGTAAGATAACAGAGCAAATATATGTTGGGTCATGTATACAAACAGAAGATGATGTGGAGGCATTGTCAAATGCTGCAGGAATTACTGCTATACTAAATTTTCAAACTGGAACCGAGACACAAAACTGGGGAATCAATTCCAATTCAATAAATGAGTCATGTCAAAAGTTTGATATTCTGATGATCAACTATCCAATAAGGGATGGAGATTCTTTTGATTTGAGGAAGAAACTACCTTTTTGCGTGGGGCTATTATTACGTTTATTGAAAAAGAATCATCGTGTTTATGTCACTTGTACCAGTGGTTTTGATCGATCTCCTACTTGTGTGATAGCATACTTGCATTGGATGACTGATACTTCCCTTCATGCAGCGTATAATTTTGTTACTGGGTTGCATTTATGTAGGCCTGACAGACCGGCAATTGCTTGGGCAACATGGGATCTTATAGCCATGGTGGAAAAAGGTAAACATGATGGACCTGCAACACATGCTGTCACATTTGTATGGAATGGACAAGAGGGGGAGGATGTAAATTTGGTTGGAGATTTCACGAATAATTGGAAAGATCCAATTCAGGCAAATCACAAAGGTGGATCACGATCGGAAGCGGAAGTTAGACTTTCACAAGGAAAGTATTACTACAAGTTCATTGTCAATGGGCAATGGCGACATTCAACATCCTCCCCAGCTGAAAGGGATGACAGCGGGAATGTCAACAATGTAATCGTAATCGGTGATACAGCCAGTGTGAGACCTTCTGTTCAACAACAGCAAAAAGATGCAAATATTATCAAGGTGATTGAGAGACCGTTGACAGAAAATGAGCGCTTCATGCTGGCAAAAGCAGCTCGTTGTGTTGCATTTTCTATATGCCCCATTAGACTGGCTCCAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001871 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005975 GO:0005976 GO:0005977 GO:0005982 GO:0005983 GO:0006073 GO:0006091 GO:0006112 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009532 GO:0009536 GO:0009569 GO:0009570 GO:0009987 GO:0012505 GO:0015980 GO:0016020 GO:0016052 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0030246 GO:0030247 GO:0030867 GO:0031090 GO:0031984 GO:0042175 GO:0042578 GO:0043036 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046838 GO:0055114 GO:0071704 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901575 GO:2001069

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMPK1_CBM DSPc

Protein Analysis

587

Amino Acids

65.22

Weight (kDa)

8.89

Isoelectric Point (pI)

38.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DSPc	PF00782	296 - 418	1.1e-10	Dual specificity phosphatase, catalytic domain
AMPK1_CBM	PF16561	454 - 535	3.8e-19	Glycogen recognition site of AMP-activated protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01510 AT3G01510 AT3G01510
fragaria_vesca	FvH4_6g50790 FvH4_6g50790
malus_domestica	MD09G1032300.v1.1
prunus_persica	Prupe.3G285700_v2.0.a1 Prupe.3G285700_v2.0.a1 Prupe.3G285700_v2.0.a1 Prupe.3G285700_v2.0.a1
pyrus_communis	pycom111g02570 pycom17g03110 pycom17g03130
rosa_chinensis	RchiOBHm_Chr2g0172101 RchiOBHm_Chr2g0172181
rosa_laevigata	RLG00000022090
rosa_multiflora	Rmu_ssc0000252.1_g000048
rosa_roxburghii	Rroxscaffold_2G00080100
rosa_rugosa	Rorug02G0556900
rosa_samantha	Rh2AG632400 Rh2BG646000 Rh2CG612600 Rh2DG653400 Rh2DG660600
rosa_wichuraiana	Rw2G052350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1354
AccBSI	CCGCTC	1 cut(s)	244
AccI	GTMKAC	2 cut(s)	270, 901
AccII	CGCG	1 cut(s)	260
AclWI	GGATC	9 cut(s)	108, 167, 244, 257, 445, 822, 1314, 1427, 1468
AcsI	RAATTY	5 cut(s)	574, 655, 781, 961, 1399
AcuI	CTGAAG	2 cut(s)	393, 443
AfaI	GTAC	2 cut(s)	221, 1162
AfeI	AGCGCT	1 cut(s)	1694
AfiI	CCNNNNNNNGG	4 cut(s)	62, 81, 565, 761
AhdI	GACNNNNNGTC	1 cut(s)	152
AjnI	CCWGG	2 cut(s)	323, 754
AjuI	GAANNNNNNNTTGG	2 cut(s)	122, 154
AleI	CACNNNNGTG	1 cut(s)	1455
AluBI	AGCT	5 cut(s)	32, 133, 490, 1556, 1715
AluI	AGCT	5 cut(s)	32, 133, 490, 1556, 1715
Alw21I	GWGCWC	1 cut(s)	34
Alw26I	GTCTC	4 cut(s)	383, 974, 1612, 1666
AlwI	GGATC	9 cut(s)	108, 167, 244, 257, 445, 822, 1314, 1427, 1468
Ama87I	CYCGRG	2 cut(s)	233, 245
Aor51HI	AGCGCT	1 cut(s)	1694
AoxI	GGCC	2 cut(s)	461, 1271
ApeKI	GCWGC	5 cut(s)	186, 490, 938, 1235, 1712
ApoI	RAATTY	5 cut(s)	574, 655, 781, 961, 1399
ArsI	GACNNNNNNTTYG	2 cut(s)	270, 302
Asp700I	GAANNNNTTC	1 cut(s)	1000
AspLEI	GCGC	3 cut(s)	262, 861, 1695
AspS9I	GGNCC	1 cut(s)	1343
AsuHPI	GGTGA	3 cut(s)	416, 1613, 1675
AvaI	CYCGRG	2 cut(s)	233, 245
AvaII	GGWCC	1 cut(s)	1343
BamHI	GGATCC	1 cut(s)	249
BanII	GRGCYC	1 cut(s)	34
BbsI	GAAGAC	1 cut(s)	437
Bbv12I	GWGCWC	1 cut(s)	34
BbvI	GCAGC	5 cut(s)	198, 477, 925, 1247, 1724
BccI	CCATC	4 cut(s)	645, 755, 1061, 1334
BceAI	ACGGC	3 cut(s)	105, 135, 289
BcgI	CGANNNNNNTGC	4 cut(s)	16, 50, 265, 299
BciT130I	CCWGG	2 cut(s)	325, 756
BclI	TGATCA	2 cut(s)	841, 1047
BcoDI	GTCTC	4 cut(s)	383, 974, 1612, 1666
BfaI	CTAG	1 cut(s)	791
BfmI	CTRYAG	2 cut(s)	862, 939
BfoI	RGCGCY	1 cut(s)	1696
BfuAI	ACCTGC	1 cut(s)	1354
Bme1390I	CCNGG	2 cut(s)	325, 756
Bme18I	GGWCC	1 cut(s)	1343
BmeRI	GACNNNNNGTC	1 cut(s)	152
BmeT110I	CYCGRG	2 cut(s)	233, 245
BmgT120I	GGNCC	1 cut(s)	1343
BmiI	GGNNCC	5 cut(s)	73, 251, 753, 976, 1755
BmrFI	CCNGG	2 cut(s)	325, 756
BmrI	ACTGGG	2 cut(s)	1000, 1263
BmsI	GCATC	2 cut(s)	408, 1636
BmuI	ACTGGG	2 cut(s)	1000, 1263
BoxI	GACNNNNGTC	1 cut(s)	92
BpiI	GAAGAC	1 cut(s)	437
BpmI	CTGGAG	1 cut(s)	777
BpuEI	CTTGAG	2 cut(s)	281, 476
Bsa29I	ATCGAT	1 cut(s)	1177
BsaBI	GATNNNNATC	1 cut(s)	1465
BsaI	GGTCTC	3 cut(s)	383, 1612, 1666
BsaJI	CCNNGG	3 cut(s)	117, 393, 1317
BsaWI	WCCGGW	1 cut(s)	135
BsaXI	ACNNNNNCTCC	2 cut(s)	914, 944
Bsc4I	CCNNNNNNNGG	4 cut(s)	62, 81, 565, 761
Bse118I	RCCGGY	1 cut(s)	1282
Bse1I	ACTGG	8 cut(s)	732, 816, 976, 995, 1164, 1258, 1611, 1755
Bse3DI	GCAATG	2 cut(s)	56, 1535
Bse8I	GATNNNNATC	1 cut(s)	1465
BseBI	CCWGG	2 cut(s)	325, 756
BseCI	ATCGAT	1 cut(s)	1177
BseDI	CCNNGG	3 cut(s)	117, 393, 1317
BseGI	GGATG	6 cut(s)	656, 1072, 1218, 1399, 1544, 1570
BseJI	GATNNNNATC	1 cut(s)	1465
BseLI	CCNNNNNNNGG	4 cut(s)	62, 81, 565, 761
BseMI	GCAATG	2 cut(s)	56, 1535
BseNI	ACTGG	8 cut(s)	732, 816, 976, 995, 1164, 1258, 1611, 1755
BseRI	GAGGAG	3 cut(s)	64, 616, 764
BseXI	GCAGC	5 cut(s)	198, 477, 925, 1247, 1724
BseYI	CCCAGC	1 cut(s)	1552
BsgI	GTGCAG	1 cut(s)	713
Bsh1236I	CGCG	1 cut(s)	260
Bsh1285I	CGRYCG	3 cut(s)	40, 96, 1469
BshFI	GGCC	2 cut(s)	463, 1273
BshVI	ATCGAT	1 cut(s)	1177
BsiEI	CGRYCG	3 cut(s)	40, 96, 1469
BsiHKAI	GWGCWC	1 cut(s)	34
BsiHKCI	CYCGRG	2 cut(s)	233, 245
BsiSI	CCGG	2 cut(s)	136, 1283
BslFI	GGGAC	1 cut(s)	100
BslI	CCNNNNNNNGG	4 cut(s)	62, 81, 565, 761
BsmAI	GTCTC	4 cut(s)	383, 974, 1612, 1666
BsmFI	GGGAC	1 cut(s)	100
BsmI	GAATGC	1 cut(s)	316
BsnI	GGCC	2 cut(s)	463, 1273
Bso31I	GGTCTC	3 cut(s)	383, 1612, 1666
BsoBI	CYCGRG	2 cut(s)	233, 245
Bsp1286I	GDGCHC	1 cut(s)	34
Bsp19I	CCATGG	1 cut(s)	1317
BspANI	GGCC	2 cut(s)	463, 1273
BspDI	ATCGAT	1 cut(s)	1177
BspFNI	CGCG	1 cut(s)	260
BspLI	GGNNCC	5 cut(s)	73, 251, 753, 976, 1755
BspMAI	CTGCAG	1 cut(s)	943
BspMI	ACCTGC	1 cut(s)	1354
BspPI	GGATC	9 cut(s)	108, 167, 244, 257, 445, 822, 1314, 1427, 1468
BspTNI	GGTCTC	3 cut(s)	383, 1612, 1666
BsrBI	CCGCTC	1 cut(s)	244
BsrDI	GCAATG	2 cut(s)	56, 1535
BsrFI	RCCGGY	1 cut(s)	1282
BsrI	ACTGG	8 cut(s)	732, 816, 976, 995, 1164, 1258, 1611, 1755
BssAI	RCCGGY	1 cut(s)	1282
BssECI	CCNNGG	3 cut(s)	117, 393, 1317
BssNAI	GTATAC	1 cut(s)	902
BssT1I	CCWWGG	2 cut(s)	393, 1317
Bst1107I	GTATAC	1 cut(s)	902
Bst2UI	CCWGG	2 cut(s)	325, 756
Bst4CI	ACNGT	4 cut(s)	154, 560, 691, 1677
Bst6I	CTCTTC	1 cut(s)	741
BstAPI	GCANNNNNTGC	1 cut(s)	1355
BstC8I	GCNNGC	2 cut(s)	44, 1704
BstDSI	CCRYGG	2 cut(s)	117, 1317
BstF5I	GGATG	6 cut(s)	656, 1072, 1218, 1399, 1544, 1570
BstFNI	CGCG	1 cut(s)	260
BstH2I	RGCGCY	1 cut(s)	1696
BstHHI	GCGC	3 cut(s)	262, 861, 1695
BstMAI	GTCTC	4 cut(s)	383, 974, 1612, 1666
BstMCI	CGRYCG	3 cut(s)	40, 96, 1469
BstMWI	GCNNNNNNNGC	6 cut(s)	52, 58, 825, 1355, 1699, 1712
BstNI	CCWGG	2 cut(s)	325, 756
BstNSI	RCATGY	1 cut(s)	1358
BstPAI	GACNNNNGTC	1 cut(s)	92
BstSCI	CCNGG	2 cut(s)	323, 754
BstSFI	CTRYAG	2 cut(s)	862, 939
BstUI	CGCG	1 cut(s)	260
BstV1I	GCAGC	5 cut(s)	198, 477, 925, 1247, 1724
BstV2I	GAAGAC	1 cut(s)	437
BstX2I	RGATCY	5 cut(s)	113, 249, 437, 1306, 1432
BstXI	CCANNNNNNTGG	2 cut(s)	651, 1750
BstYI	RGATCY	5 cut(s)	113, 249, 437, 1306, 1432
BstZ17I	GTATAC	1 cut(s)	902
Bsu15I	ATCGAT	1 cut(s)	1177
BsuRI	GGCC	2 cut(s)	463, 1273
BsuTUI	ATCGAT	1 cut(s)	1177
BtgI	CCRYGG	2 cut(s)	117, 1317
BtgZI	GCGATG	1 cut(s)	182
BtsCI	GGATG	6 cut(s)	656, 1072, 1218, 1399, 1544, 1570
BtsI	GCAGTG	1 cut(s)	604
BtsIMutI	CAGTG	5 cut(s)	604, 696, 809, 1171, 1618
BveI	ACCTGC	1 cut(s)	1354
Cac8I	GCNNGC	2 cut(s)	44, 1704
CfoI	GCGC	3 cut(s)	262, 861, 1695
Cfr10I	RCCGGY	1 cut(s)	1282
Cfr13I	GGNCC	1 cut(s)	1343
ClaI	ATCGAT	1 cut(s)	1177
Csp6I	GTAC	2 cut(s)	220, 1161
CviQI	GTAC	2 cut(s)	220, 1161
DriI	GACNNNNNGTC	1 cut(s)	152
Eam1104I	CTCTTC	1 cut(s)	741
Eam1105I	GACNNNNNGTC	1 cut(s)	152
EarI	CTCTTC	1 cut(s)	741
EciI	GGCGGA	1 cut(s)	137
Ecl136II	GAGCTC	1 cut(s)	32
Eco130I	CCWWGG	2 cut(s)	393, 1317
Eco147I	AGGCCT	1 cut(s)	1273
Eco24I	GRGCYC	1 cut(s)	34
Eco31I	GGTCTC	3 cut(s)	383, 1612, 1666
Eco47I	GGWCC	1 cut(s)	1343
Eco47III	AGCGCT	1 cut(s)	1694
Eco53kI	GAGCTC	1 cut(s)	32
Eco57I	CTGAAG	2 cut(s)	393, 443
Eco88I	CYCGRG	2 cut(s)	233, 245
EcoICRI	GAGCTC	1 cut(s)	32
EcoRI	GAATTC	1 cut(s)	655
EcoRII	CCWGG	2 cut(s)	323, 754
EcoT14I	CCWWGG	2 cut(s)	393, 1317
EcoT38I	GRGCYC	1 cut(s)	34
ErhI	CCWWGG	2 cut(s)	393, 1317
FalI	AAGNNNNNCTT	6 cut(s)	194, 226, 366, 398, 1470, 1502
FaqI	GGGAC	1 cut(s)	100
FauI	CCCGC	2 cut(s)	352, 1565
FbaI	TGATCA	2 cut(s)	841, 1047
FblI	GTMKAC	2 cut(s)	270, 901
FokI	GGATG	6 cut(s)	663, 1079, 1225, 1406, 1531, 1577
FriOI	GRGCYC	1 cut(s)	34
FspBI	CTAG	1 cut(s)	791
GlaI	GCGC	3 cut(s)	261, 860, 1694
GsaI	CCCAGC	1 cut(s)	1556
GsuI	CTGGAG	1 cut(s)	777
HaeII	RGCGCY	1 cut(s)	1696
HaeIII	GGCC	2 cut(s)	463, 1273
HapII	CCGG	2 cut(s)	136, 1283
HhaI	GCGC	3 cut(s)	262, 861, 1695
Hin6I	GCGC	3 cut(s)	260, 859, 1693
HinP1I	GCGC	3 cut(s)	260, 859, 1693
HincII	GTYRAC	3 cut(s)	271, 1582, 1680
HindII	GTYRAC	3 cut(s)	271, 1582, 1680
HinfI	GANTC	4 cut(s)	996, 1019, 1073, 1138
HpaII	CCGG	2 cut(s)	136, 1283
HphI	GGTGA	3 cut(s)	416, 1613, 1675
Hpy166II	GTNNAC	7 cut(s)	6, 271, 289, 902, 1334, 1582, 1680
Hpy188I	TCNGA	5 cut(s)	21, 373, 745, 1044, 1471
Hpy8I	GTNNAC	7 cut(s)	6, 271, 289, 902, 1334, 1582, 1680
Hpy99I	CGWCG	5 cut(s)	178, 181, 184, 272, 275
HpyAV	CCTTC	4 cut(s)	368, 418, 1238, 1632
HpyCH4III	ACNGT	4 cut(s)	154, 560, 691, 1677
HpyCH4IV	ACGT	2 cut(s)	620, 1123
HpyF10VI	GCNNNNNNNGC	6 cut(s)	52, 58, 825, 1355, 1699, 1712
HpySE526I	ACGT	2 cut(s)	620, 1123
HspAI	GCGC	3 cut(s)	260, 859, 1693
Ksp22I	TGATCA	2 cut(s)	841, 1047
LmnI	GCTCC	4 cut(s)	77, 138, 751, 1759
Lsp1109I	GCAGC	5 cut(s)	198, 477, 925, 1247, 1724
LweI	GCATC	2 cut(s)	408, 1636
MaeI	CTAG	1 cut(s)	791
MaeII	ACGT	2 cut(s)	620, 1123
MaeIII	GTNAC	5 cut(s)	226, 616, 1153, 1249, 1360
MbiI	CCGCTC	1 cut(s)	244
MboII	GAAGA	8 cut(s)	139, 274, 427, 442, 758, 806, 923, 1102
MfeI	CAATTG	1 cut(s)	1287
MflI	RGATCY	5 cut(s)	113, 249, 437, 1306, 1432
MhlI	GDGCHC	1 cut(s)	34
MlyI	GAGTC	1 cut(s)	1028
MmeI	TCCRAC	2 cut(s)	120, 1387
MroXI	GAANNNNTTC	1 cut(s)	1000
MseI	TTAA	1 cut(s)	804
MslI	CAYNNNNRTG	3 cut(s)	649, 1369, 1455
MspA1I	CMGCKG	3 cut(s)	490, 1556, 1572
MspI	CCGG	2 cut(s)	136, 1283
MspR9I	CCNGG	2 cut(s)	325, 756
MunI	CAATTG	1 cut(s)	1287
Mva1269I	GAATGC	1 cut(s)	316
MvaI	CCWGG	2 cut(s)	325, 756
MvnI	CGCG	1 cut(s)	260
MwoI	GCNNNNNNNGC	6 cut(s)	52, 58, 825, 1355, 1699, 1712
NcoI	CCATGG	1 cut(s)	1317
NlaIV	GGNNCC	5 cut(s)	73, 251, 753, 976, 1755
NmeAIII	GCCGAG	1 cut(s)	42
NmuCI	GTSAC	4 cut(s)	226, 616, 1153, 1360
NspI	RCATGY	1 cut(s)	1358
OliI	CACNNNNGTG	1 cut(s)	1455
PaeR7I	CTCGAG	1 cut(s)	233
PceI	AGGCCT	1 cut(s)	1273
PcsI	WCGNNNNNNNCGW	2 cut(s)	179, 264
PctI	GAATGC	1 cut(s)	316
PdmI	GAANNNNTTC	1 cut(s)	1000
PfeI	GAWTC	3 cut(s)	996, 1073, 1138
PfoI	TCCNGGA	1 cut(s)	323
Ple19I	CGATCG	2 cut(s)	40, 1469
PleI	GAGTC	1 cut(s)	1027
PpsI	GAGTC	1 cut(s)	1027
PshAI	GACNNNNGTC	1 cut(s)	92
Psp124BI	GAGCTC	1 cut(s)	34
Psp6I	CCWGG	2 cut(s)	323, 754
PspFI	CCCAGC	1 cut(s)	1552
PspGI	CCWGG	2 cut(s)	323, 754
PspN4I	GGNNCC	5 cut(s)	73, 251, 753, 976, 1755
PspPI	GGNCC	1 cut(s)	1343
PstI	CTGCAG	1 cut(s)	943
PsuI	RGATCY	5 cut(s)	113, 249, 437, 1306, 1432
PvuI	CGATCG	2 cut(s)	40, 1469
PvuII	CAGCTG	2 cut(s)	490, 1556
RsaI	GTAC	2 cut(s)	221, 1162
RsaNI	GTAC	2 cut(s)	220, 1161
RseI	CAYNNNNRTG	3 cut(s)	649, 1369, 1455
SacI	GAGCTC	1 cut(s)	34
SalI	GTCGAC	1 cut(s)	269
SaqAI	TTAA	1 cut(s)	804
Sau96I	GGNCC	1 cut(s)	1343
SchI	GAGTC	1 cut(s)	1028
ScrFI	CCNGG	2 cut(s)	325, 756
SduI	GDGCHC	1 cut(s)	34
SfaNI	GCATC	2 cut(s)	408, 1636
SfcI	CTRYAG	2 cut(s)	862, 939
Sfr274I	CTCGAG	1 cut(s)	233
SgrDI	CGTCGACG	1 cut(s)	269
SinI	GGWCC	1 cut(s)	1343
SlaI	CTCGAG	1 cut(s)	233
SmiMI	CAYNNNNRTG	3 cut(s)	649, 1369, 1455
SmlI	CTYRAG	3 cut(s)	233, 296, 455
SmoI	CTYRAG	3 cut(s)	233, 296, 455
SseBI	AGGCCT	1 cut(s)	1273
SspI	AATATT	1 cut(s)	1654
SspMI	CTAG	1 cut(s)	791
SstI	GAGCTC	1 cut(s)	34
StuI	AGGCCT	1 cut(s)	1273
StyD4I	CCNGG	2 cut(s)	323, 754
StyI	CCWWGG	2 cut(s)	393, 1317
TaaI	ACNGT	4 cut(s)	154, 560, 691, 1677
TaiI	ACGT	2 cut(s)	623, 1126
TaqI	TCGA	6 cut(s)	36, 182, 234, 270, 532, 1177
TatI	WGTACW	1 cut(s)	219
TauI	GCSGC	6 cut(s)	49, 52, 55, 69, 72, 244
TfiI	GAWTC	3 cut(s)	996, 1073, 1138
Tru1I	TTAA	1 cut(s)	804
Tru9I	TTAA	1 cut(s)	804
TscAI	CASTG	5 cut(s)	604, 696, 816, 1171, 1618
TseFI	GTSAC	4 cut(s)	226, 616, 1153, 1360
TseI	GCWGC	5 cut(s)	186, 490, 938, 1235, 1712
Tsp45I	GTSAC	4 cut(s)	226, 616, 1153, 1360
TspDTI	ATGAA	6 cut(s)	221, 357, 515, 1220, 1504, 1687
TspGWI	ACGGA	5 cut(s)	159, 163, 242, 256, 792
TspRI	CASTG	5 cut(s)	604, 696, 816, 1171, 1618
VpaK11BI	GGWCC	1 cut(s)	1343
XapI	RAATTY	5 cut(s)	574, 655, 781, 961, 1399
XceI	RCATGY	1 cut(s)	1358
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 724
XhoI	CTCGAG	1 cut(s)	233
XmiI	GTMKAC	2 cut(s)	270, 901
XmnI	GAANNNNTTC	1 cut(s)	1000
XspI	CTAG	1 cut(s)	791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.