Rh3AG280100

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0016173
Physical Location & Seq
Forward (+)
32617771 .. 32644129
26359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG280100.1

Sequence Viewer

Length: 1548 bp
ATGGATTGGAAGCTAAGCAAACATAGGTATCAAGGTTCAGCGTTAGAGCCTGGGAAGCTGGATTGGCACGGAGACCCACAGCAGCAGTCCCAGCACCATGGGACTTGCTCTTCCTCTTCTGCTTGGCCACCCCTCGATCCCCACCACAACCCTCATAACATTCCCCACCATCCTATTCCTCCTCACCAGCTCGACTACCATCCCCACCACCACGATCCCAACCACGCCACTGGCTTCGCCAATCCGGTGCCGTTTTCTGTACGCAAAAGGGCTTGGCAACACTCTAATCAAGGACTTCCTAGAGATAGCTTTGAAAACCCCAATCATGCTAAGCTTTATGTTGCACAAATTTCAAGAACAGCCACGGAGGACGTTATTCGCTCCTTGTTTGGACAATTTGGGGACATAGTCGAGGTTGTAATGTTGAAGGATAAGCGGACTGGCTTCCAACAAGGAAATTGTTTCGTGAAGTATTCAACATTAGATGCAGCAGAGAGGGCTATCAGAGCTTTAAATAGTCAGCATTATTTTCCTGGGGAGGCAAACCCTATCATAGTCAAATTTGCTGACGGGGAAAGGGAACGCCTTGGGGTACAAGACAGATTGTATGTTTGTGGCATTAACCTGGAAGCTTCAAATAAAGACATTGAGGAAATATTTTCACCTTATGGTCTTGTTGAAGATATCTACATTTTGAAGCACAATCGTGGATGCGGGTTTGTTAAATTTTCCAGTGGAGATATGGCTGTGCAAGCAATCAAAGCTTTAAATGGAACCTTTATTATGAGAGGTTGTGCTCAGCCATTGATTGTTCGTTTTGCGGATCCTAAGAAACCAAGGGAAATAGATCGAAGGGGTAATTATGTATTTAGCAGTGCACCTGGTGGTCCGTACTCTCACGAACCAGTTAGGGTAGTATCTGATCATAGTGATTCCATTGGAGGACAAAATTTTCTAAATGCATCTTATCCTGTCCAACAAATTTCACAACCCCCTATTCCCTATTTGGCAAACCAGGAACCACAGGCTTCTACAGTGATGCAACCAACGTTTCCTCCATTAAGACCACCTTTACAGTTGTGCAGGATGCCTATACAACAAACACAGAATACACAAAGAAGTTCTCAGTCTTCTCAAGTAGCAGTCACGGAGATGCAGAAACAGCATCTGACACAATCATCTGGGCAAATTCTGGGGCAGCAACAGAGTTCTCAGACATTTTCTAGCAATTCTACTTCAGCTGCGCTGCCTTCAAGTCATGAGATAACTGCTCCTCTAGAGTGTGACTGGAGTGAACATACCTGCCCTGATGGATACAAGTATTATTATAATTGTGAAACTTGTGAGAGCAGATGGGACAAACCTGAGGAGTTTGCTTCATTTGAGCAACAATTGCAGATGCAAACACAGCCGAATAATCCTTCTCATCATCTTCAATCCGTGTCATTGGTTCCTTCTACGCAAATAGTTTGTCAAAATCAGCAGGAACTGAATCAAGTACAATTGCAGTCTGAAACAAGTCCTGTTGTTGGCCCAACATGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 WW

Protein Analysis

515

Amino Acids

57.81

Weight (kDa)

6.35

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 112 - 176 1.8e-14 RNA recognition motif
RRM_1 PF00076 202 - 262 1.2e-12 RNA recognition motif
WW PF00397 426 - 455 1.2e-09 WW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47310
fragaria_vesca FvH4_1g26970
malus_domestica MD09G1285500.v1.1
prunus_persica Prupe.3G008500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487191
rosa_multiflora Rmu_sc0012751.1_g000001 Rmu_ssc0000366.1_g000028
rosa_roxburghii Rroxscaffold_6G00395040
rosa_rugosa Rorug03G0232100 Rorug03G0232200
rosa_samantha Rh3AG280100 Rh3BG316100 Rh3CG314200 Rh3DG311500
rosa_wichuraiana Rw3G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1329
Acc36I ACCTGC 1 cut(s) 1310
AccB1I GGYRCC 1 cut(s) 247
AciI CCGC 3 cut(s) 436, 714, 821
AclI AACGTT 1 cut(s) 1049
AclWI GGATC 4 cut(s) 131, 209, 818, 831
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 6 cut(s) 348, 560, 725, 949, 981, 1188
AcuI CTGAAG 1 cut(s) 1221
AdeI CACNNNGTG 1 cut(s) 884
AfaI GTAC 4 cut(s) 261, 594, 893, 1500
AfiI CCNNNNNNNGG 1 cut(s) 1529
AflIII ACRYGT 1 cut(s) 1538
AgsI TTSAA 9 cut(s) 314, 354, 427, 477, 636, 680, 697, 1254, 1436
AjnI CCWGG 5 cut(s) 49, 532, 624, 880, 1014
AleI CACNNNNGTG 1 cut(s) 705
AluBI AGCT 9 cut(s) 13, 58, 190, 309, 334, 509, 632, 764, 1241
AluI AGCT 9 cut(s) 13, 58, 190, 309, 334, 509, 632, 764, 1241
Alw21I GWGCWC 2 cut(s) 799, 880
Alw26I GTCTC 1 cut(s) 66
Alw44I GTGCAC 1 cut(s) 876
AlwI GGATC 4 cut(s) 131, 209, 818, 831
AlwNI CAGNNNCTG 2 cut(s) 1168, 1489
AoxI GGCC 2 cut(s) 125, 1531
ApaLI GTGCAC 1 cut(s) 876
ApeKI GCWGC 5 cut(s) 82, 488, 1198, 1241, 1246
ApoI RAATTY 6 cut(s) 348, 560, 725, 949, 981, 1188
ArsI GACNNNNNNTTYG 2 cut(s) 1456, 1488
AspLEI GCGC 1 cut(s) 1246
AspS9I GGNCC 2 cut(s) 887, 1532
AsuHPI GGTGA 2 cut(s) 176, 654
AvaII GGWCC 1 cut(s) 887
AxyI CCTNAGG 1 cut(s) 1365
BaeGI GKGCMC 1 cut(s) 880
BaeI ACNNNNGTAYC 2 cut(s) 11, 44
BalI TGGCCA 1 cut(s) 127
BamHI GGATCC 1 cut(s) 823
BanI GGYRCC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 1122
Bbv12I GWGCWC 2 cut(s) 799, 880
BbvI GCAGC 5 cut(s) 94, 500, 1210, 1228, 1233
BccI CCATC 4 cut(s) 177, 207, 1304, 1347
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 5 cut(s) 51, 534, 626, 882, 1016
BciVI GTATCC 1 cut(s) 1307
BclI TGATCA 1 cut(s) 922
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 3 cut(s) 300, 1224, 1277
BfmI CTRYAG 1 cut(s) 1032
BfuAI ACCTGC 1 cut(s) 1310
BfuI GTATCC 1 cut(s) 1307
BisI GCNGC 5 cut(s) 83, 489, 1199, 1242, 1247
BlpI GCTNAGC 3 cut(s) 14, 330, 798
BlsI GCNGC 5 cut(s) 84, 490, 1200, 1243, 1248
Bme1390I CCNGG 5 cut(s) 51, 534, 626, 882, 1016
Bme18I GGWCC 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 887, 1532
BmiI GGNNCC 5 cut(s) 249, 775, 825, 1020, 1452
BmrFI CCNGG 5 cut(s) 51, 534, 626, 882, 1016
BmsI GCATC 8 cut(s) 475, 701, 971, 1029, 1077, 1143, 1174, 1389
BpiI GAAGAC 1 cut(s) 1122
BpmI CTGGAG 1 cut(s) 1309
Bpu1102I GCTNAGC 3 cut(s) 14, 330, 798
BpuEI CTTGAG 1 cut(s) 1119
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 6 cut(s) 50, 97, 363, 533, 586, 836
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 1039, 1069
Bsc4I CCNNNNNNNGG 1 cut(s) 1529
Bse1I ACTGG 5 cut(s) 235, 445, 732, 905, 1292
Bse21I CCTNAGG 1 cut(s) 1365
BseBI CCWGG 5 cut(s) 51, 534, 626, 882, 1016
BseDI CCNNGG 6 cut(s) 50, 97, 363, 533, 586, 836
BseGI GGATG 4 cut(s) 169, 199, 716, 1092
BseLI CCNNNNNNNGG 1 cut(s) 1529
BseMII CTCAG 4 cut(s) 812, 1139, 1226, 1356
BseNI ACTGG 5 cut(s) 235, 445, 732, 905, 1292
BseRI GAGGAG 3 cut(s) 171, 1263, 1382
BseSI GKGCMC 1 cut(s) 880
BseXI GCAGC 5 cut(s) 94, 500, 1210, 1228, 1233
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 1 cut(s) 1102
BshFI GGCC 2 cut(s) 127, 1533
BshNI GGYRCC 1 cut(s) 247
BsiHKAI GWGCWC 2 cut(s) 799, 880
BsiSI CCGG 1 cut(s) 245
BslFI GGGAC 4 cut(s) 73, 115, 416, 1370
BslI CCNNNNNNNGG 1 cut(s) 1529
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 4 cut(s) 73, 115, 416, 1370
BsnI GGCC 2 cut(s) 127, 1533
Bso31I GGTCTC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 799, 880
Bsp143I GATC 5 cut(s) 136, 214, 823, 847, 922
Bsp1720I GCTNAGC 3 cut(s) 14, 330, 798
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 3 cut(s) 436, 714, 821
BspANI GGCC 2 cut(s) 127, 1533
BspCNI CTCAG 4 cut(s) 811, 1138, 1225, 1357
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 5 cut(s) 249, 775, 825, 1020, 1452
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 4 cut(s) 131, 209, 818, 831
BspQI GCTCTTC 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 66
BsrI ACTGG 5 cut(s) 235, 445, 732, 905, 1292
BssECI CCNNGG 6 cut(s) 50, 97, 363, 533, 586, 836
BssMI GATC 5 cut(s) 136, 214, 823, 847, 922
BssT1I CCWWGG 3 cut(s) 97, 586, 836
Bst2UI CCWGG 5 cut(s) 51, 534, 626, 882, 1016
Bst4CI ACNGT 2 cut(s) 1036, 1077
Bst6I CTCTTC 2 cut(s) 115, 121
BstAPI GCANNNNNTGC 1 cut(s) 1393
BstC8I GCNNGC 1 cut(s) 753
BstDEI CTNAG 7 cut(s) 14, 330, 798, 828, 1125, 1212, 1365
BstDSI CCRYGG 2 cut(s) 97, 363
BstF5I GGATG 4 cut(s) 169, 199, 716, 1092
BstHHI GCGC 1 cut(s) 1246
BstKTI GATC 5 cut(s) 139, 217, 826, 850, 925
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 5 cut(s) 136, 214, 823, 847, 922
BstNI CCWGG 5 cut(s) 51, 534, 626, 882, 1016
BstNSI RCATGY 1 cut(s) 1542
BstSCI CCNGG 5 cut(s) 49, 532, 624, 880, 1014
BstSFI CTRYAG 1 cut(s) 1032
BstSLI GKGCMC 1 cut(s) 880
BstV1I GCAGC 5 cut(s) 94, 500, 1210, 1228, 1233
BstV2I GAAGAC 1 cut(s) 1122
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 2 cut(s) 98, 230
BstYI RGATCY 1 cut(s) 823
Bsu36I CCTNAGG 1 cut(s) 1365
BsuI GTATCC 1 cut(s) 1307
BsuRI GGCC 2 cut(s) 127, 1533
BtgI CCRYGG 2 cut(s) 97, 363
BtsCI GGATG 4 cut(s) 169, 199, 716, 1092
BtsI GCAGTG 1 cut(s) 880
BtsIMutI CAGTG 4 cut(s) 228, 739, 880, 1041
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 1 cut(s) 753
CaiI CAGNNNCTG 2 cut(s) 1168, 1489
CciI TCATGA 1 cut(s) 1258
CfoI GCGC 1 cut(s) 1246
Cfr13I GGNCC 2 cut(s) 887, 1532
CsiI ACCWGGT 1 cut(s) 880
Csp6I GTAC 4 cut(s) 260, 593, 892, 1499
CviAII CATG 4 cut(s) 98, 326, 1259, 1539
CviQI GTAC 4 cut(s) 260, 593, 892, 1499
DdeI CTNAG 7 cut(s) 14, 330, 798, 828, 1125, 1212, 1365
DpnI GATC 5 cut(s) 138, 216, 825, 849, 924
DpnII GATC 5 cut(s) 136, 214, 823, 847, 922
DraI TTTAAA 2 cut(s) 513, 768
DraIII CACNNNGTG 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 2 cut(s) 115, 121
EarI CTCTTC 2 cut(s) 115, 121
Eco130I CCWWGG 3 cut(s) 97, 586, 836
Eco31I GGTCTC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 1221
Eco81I CCTNAGG 1 cut(s) 1365
EcoRII CCWGG 5 cut(s) 49, 532, 624, 880, 1014
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 3 cut(s) 97, 586, 836
EcoT22I ATGCAT 1 cut(s) 964
ErhI CCWWGG 3 cut(s) 97, 586, 836
FaeI CATG 4 cut(s) 101, 329, 1262, 1542
FalI AAGNNNNNCTT 2 cut(s) 1054, 1086
FaqI GGGAC 4 cut(s) 73, 115, 416, 1370
FatI CATG 4 cut(s) 97, 325, 1258, 1538
FauI CCCGC 1 cut(s) 707
FbaI TGATCA 1 cut(s) 922
Fnu4HI GCNGC 5 cut(s) 83, 489, 1199, 1242, 1247
FokI GGATG 4 cut(s) 156, 186, 723, 1099
Fsp4HI GCNGC 5 cut(s) 83, 489, 1199, 1242, 1247
FspBI CTAG 3 cut(s) 300, 1224, 1277
GlaI GCGC 1 cut(s) 1245
GluI GCNGC 5 cut(s) 83, 489, 1199, 1242, 1247
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 1 cut(s) 1309
HaeIII GGCC 2 cut(s) 127, 1533
HapII CCGG 1 cut(s) 245
HhaI GCGC 1 cut(s) 1246
Hin1II CATG 4 cut(s) 101, 329, 1262, 1542
Hin6I GCGC 1 cut(s) 1244
HinP1I GCGC 1 cut(s) 1244
HindIII AAGCTT 3 cut(s) 332, 630, 762
HinfI GANTC 2 cut(s) 932, 1492
HpaII CCGG 1 cut(s) 245
HphI GGTGA 2 cut(s) 176, 654
Hpy166II GTNNAC 2 cut(s) 878, 1295
Hpy188I TCNGA 5 cut(s) 506, 922, 1170, 1215, 1513
Hpy188III TCNNGA 5 cut(s) 354, 466, 899, 1259, 1277
Hpy8I GTNNAC 2 cut(s) 878, 1295
HpyAV CCTTC 5 cut(s) 421, 846, 1260, 1431, 1464
HpyCH4III ACNGT 2 cut(s) 1036, 1077
HpyCH4IV ACGT 2 cut(s) 372, 1049
HpyF3I CTNAG 7 cut(s) 14, 330, 798, 828, 1125, 1212, 1365
HpySE526I ACGT 2 cut(s) 372, 1049
Hsp92II CATG 4 cut(s) 101, 329, 1262, 1542
HspAI GCGC 1 cut(s) 1244
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 5 cut(s) 136, 214, 823, 847, 922
LguI GCTCTTC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 386, 1276
Lsp1109I GCAGC 5 cut(s) 94, 500, 1210, 1228, 1233
LweI GCATC 8 cut(s) 475, 701, 971, 1029, 1077, 1143, 1174, 1389
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 3 cut(s) 300, 1224, 1277
MaeII ACGT 2 cut(s) 372, 1049
MaeIII GTNAC 2 cut(s) 1144, 1283
MalI GATC 5 cut(s) 138, 216, 825, 849, 924
MboI GATC 5 cut(s) 136, 214, 823, 847, 922
MboII GAAGA 5 cut(s) 102, 108, 692, 1122, 1424
MfeI CAATTG 2 cut(s) 1391, 1502
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 2 cut(s) 799, 880
MlsI TGGCCA 1 cut(s) 127
MluNI TGGCCA 1 cut(s) 127
MmeI TCCRAC 2 cut(s) 472, 1000
Mox20I TGGCCA 1 cut(s) 127
Mph1103I ATGCAT 1 cut(s) 964
MscI TGGCCA 1 cut(s) 127
MseI TTAA 5 cut(s) 512, 621, 723, 767, 1061
MslI CAYNNNNRTG 2 cut(s) 705, 1151
Msp20I TGGCCA 1 cut(s) 127
MspA1I CMGCKG 1 cut(s) 1241
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 5 cut(s) 51, 534, 626, 882, 1016
MunI CAATTG 2 cut(s) 1391, 1502
MvaI CCWGG 5 cut(s) 51, 534, 626, 882, 1016
NcoI CCATGG 1 cut(s) 97
NdeII GATC 5 cut(s) 136, 214, 823, 847, 922
NlaIII CATG 4 cut(s) 101, 329, 1262, 1542
NlaIV GGNNCC 5 cut(s) 249, 775, 825, 1020, 1452
NmuCI GTSAC 2 cut(s) 1144, 1283
NsiI ATGCAT 1 cut(s) 964
NspI RCATGY 1 cut(s) 1542
OliI CACNNNNGTG 1 cut(s) 705
PagI TCATGA 1 cut(s) 1258
PciI ACATGT 1 cut(s) 1538
PciSI GCTCTTC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 932, 1492
PflFI GACNNNGTC 1 cut(s) 407
PkrI GCNGC 5 cut(s) 84, 490, 1200, 1243, 1248
PscI ACATGT 1 cut(s) 1538
PsiI TTATAA 1 cut(s) 1329
Psp1406I AACGTT 1 cut(s) 1049
Psp6I CCWGG 5 cut(s) 49, 532, 624, 880, 1014
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 5 cut(s) 49, 532, 624, 880, 1014
PspN4I GGNNCC 5 cut(s) 249, 775, 825, 1020, 1452
PspPI GGNCC 2 cut(s) 887, 1532
PstNI CAGNNNCTG 2 cut(s) 1168, 1489
PsuI RGATCY 1 cut(s) 823
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 1241
RsaI GTAC 4 cut(s) 261, 594, 893, 1500
RsaNI GTAC 4 cut(s) 260, 593, 892, 1499
RseI CAYNNNNRTG 2 cut(s) 705, 1151
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 5 cut(s) 512, 621, 723, 767, 1061
SatI GCNGC 5 cut(s) 83, 489, 1199, 1242, 1247
Sau3AI GATC 5 cut(s) 136, 214, 823, 847, 922
Sau96I GGNCC 2 cut(s) 887, 1532
ScrFI CCNGG 5 cut(s) 51, 534, 626, 882, 1016
SduI GDGCHC 2 cut(s) 799, 880
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 8 cut(s) 475, 701, 971, 1029, 1077, 1143, 1174, 1389
SfcI CTRYAG 1 cut(s) 1032
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 2 cut(s) 705, 1151
SmlI CTYRAG 1 cut(s) 1134
SmoI CTYRAG 1 cut(s) 1134
SsiI CCGC 3 cut(s) 436, 714, 821
SspI AATATT 1 cut(s) 657
SspMI CTAG 3 cut(s) 300, 1224, 1277
StyD4I CCNGG 5 cut(s) 49, 532, 624, 880, 1014
StyI CCWWGG 3 cut(s) 97, 586, 836
TaaI ACNGT 2 cut(s) 1036, 1077
TaiI ACGT 2 cut(s) 375, 1052
TaqI TCGA 4 cut(s) 135, 192, 411, 850
TatI WGTACW 1 cut(s) 1498
TfiI GAWTC 2 cut(s) 932, 1492
Tru1I TTAA 5 cut(s) 512, 621, 723, 767, 1061
Tru9I TTAA 5 cut(s) 512, 621, 723, 767, 1061
TscAI CASTG 4 cut(s) 235, 739, 880, 1041
TseFI GTSAC 2 cut(s) 1144, 1283
TseI GCWGC 5 cut(s) 82, 488, 1198, 1241, 1246
Tsp45I GTSAC 2 cut(s) 1144, 1283
TspDTI ATGAA 1 cut(s) 1368
TspGWI ACGGA 5 cut(s) 84, 380, 879, 1163, 1429
TspRI CASTG 4 cut(s) 235, 739, 880, 1041
Tth111I GACNNNGTC 1 cut(s) 407
VneI GTGCAC 1 cut(s) 876
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 6 cut(s) 348, 560, 725, 949, 981, 1188
XbaI TCTAGA 1 cut(s) 1276
XceI RCATGY 1 cut(s) 1542
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XspI CTAG 3 cut(s) 300, 1224, 1277
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.