Rh3DG085600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0018505
Physical Location & Seq
Forward (+)
6678777 .. 6681110
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG085600.1

Sequence Viewer

Length: 2334 bp
ATGAACTTGGCTAGATTGACCATAAACTTGTCAATTTTAGAAACCCATTTGCCCAAATGCAAGAATCTCAAGCAGTTCAACCAAATACTATCTCAGATGATCCTCACTGGTTTCATTAGGGACACATATGCTGCAAGTAGAGTCCTCAAGTTCTCCACTGACTCTTCTTTCAGTGACATTGATTTGTCGTTTAGAATTTTCAAACACATTGAGAACTCAAATGGGTTCATTTGGAACACTATGATGAAAGCTTACATACAAAGAAACTGTCCCCAAAAGACTTTGGGTCTTTACAAATTGATGTTGGGTAAGAATGGGGATAGAGATAATTATACATACCCAATCCTAGTTCAAGCTTGTGCTATGAGAGCATCAGAGTTTGAAGGGAAACAGATGCATAGTCATATTTTGAAGTTTGGGTTTGATTCAGACGTGTATGTGCAGAACACATTGATCAACATGTACTCAGTTTGTGACAATATGAGGGATGCACGCAATGTGTTTGATGAAATGCCTGTGTTGGACTCGGTGGCGTGGAATTCGATATTGGCTGGCTATGTTAGGGTGGGGGATGTAAAGCAGGCTAAGTGTATATATGATCAGATGCCAGTGAGGAACACGATTGCTTCGAATTCTATGATTGTGTTGTTTGGTAAAAGAGGTTGTGTGACTGAAGCTCGGCATTTGTTTGATGAACTGCCTGAGAAGGATATGGTTTCGTGGAGTGCGTTGATTTCTTGTTTCGAGCAAAATGAGATGTATGAGGCGGCTTTGGATATGTTTGTGAAAATGGTTGCTAATGGAGTTATGGTGGATGAGGTTGTGGTGGTTAGTGTGCTTTCGGCATGTGCACATTTGTCAGCTGTTCACACAGGGAAGTTGATCCATGGGTTAGTTGTGAAAATTGGAATTGAAAGTTATGTTAATCTTCAAAATGCTTTTATTCATATGTATTCCAGTTGTGGGGAGATAATGGTTGCCGAGAAACTGTTCAATGCAGCCTACAACCTGGATCAGATATCTTGGAACTCAATGATATCGGGGTACTTGAGATGTGGTTTAGTTGAAGCTGCTAAGACATTATTTGATTCCATGCCTGAGAAGGATGTAGTGTCTTGGAGTGCAATGATATCAGGCTATGCTCAGCATGACCGGTTTTCAGAAACTTTGGCATTGTTCCAGGAGATGCAGCTGTGTGGAATTAGGCCCGATGAGACCACTTTGGTGAGCGTCATCTCGGCTTGCACTCATTTGGCTGCCCTTGACCTGGGCCAATGGATTCATGCTTATATAAGGAAAAATAGCCTTAAAGTTAATGTCTTTCTTGGGACAACCCTTATTAACATGTACTTGAAATGTGGGTGTGTGGAAAATGCATTGGAGGTTTTCGAGGAGACAGCAGAAAAGGGAGTTTCTACCTGGAATGCTCTTATTCTTGGGTTGGCAATGAATGGGTTGGTGGAGAAGTCGCTTGAAATGTTTGCAAAGATGAAGACAAGGGGTGTGACACCTAATGAAATAACCTTTATGGGAGTTATTGGAGCTTGTCGTCACATGGGCTTGGTAGATGAGGGGCGTCGCCACTTCGATTCCATGATGCAGGAACACAAGATAGAACCCAATGTTAAGCATTACGGATGCATGGTTGATCTTCTTGGACGTGCTGGTATGCTCAAGGAGGCAGAGGACCTCATTGAAAGTATGCCTATGACACCAGATGTTGCTACTTGGGGTGCCTTACTGGGGGCTTGTAAGAAACATGGGGACCATGACAGGGGTGAGAGGATAGGAAGAAAGCTCATTGAGCTCGATCCTAACCATGATGGGTTCCATGTGTTGTTGTCCAATATATGTGCTTCGAAAGGTGATTGGGATGGTGTTCATGAGATTAGGGGAATAATGATGCAACATGGGGTGGTAAAGACCCCAGGTTGTAGCATGATCGAAGCAGATGGTGTAGTTCATGAATTTCTAGCAGGAGATAAGAAACACCCTCGAATGAAAGATATTGAGGTTATGCTAGATGAAATGGCTCAGAAATTGAAGAGGGAAGGTTATGCACCAGATACAAATGAGGTATCCTTTGACATTGATGAAGAGGAAAAGGAAACTGTTCTTTTCAGACACAGTGAGAAACTGGCAATTGCCTTTGGGCTAATCGCTATCAGTCCACCATCACCAATACGAATAATAAAGAATTTGCGAATATGTAATGATTGTCACATGGCAGCAAAGTTCGTCTCGAAAGCTTTTGGTCGTGACATTGTGGTGAGGGATAAGCACCGCTTTCACCACTTTAAGCAGGGAGCTTGTTCCTGCAATGACTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

777

Amino Acids

87.45

Weight (kDa)

6.19

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 76 - 121 6.7e-07 PPR repeat family
PPR PF01535 340 - 369 1.2e-07 PPR repeat
PPR_2 PF13041 368 - 416 7.2e-10 PPR repeat family
PPR PF01535 371 - 401 8.7e-07 PPR repeat
PPR_2 PF13041 470 - 516 3.3e-10 PPR repeat family
PPR PF01535 472 - 502 1.2e-06 PPR repeat
E_motif PF20431 587 - 649 2.1e-13 E motif
DYW_deaminase PF14432 685 - 777 3.3e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018505)

Species Orthologous Gene IDs
malus_domestica MD04G1191500.v1.1
prunus_persica Prupe.6G310100_v2.0.a1
pyrus_communis pycom04g16910
rosa_chinensis RchiOBHm_Chr3g0457551
rosa_laevigata RLG00000025211
rosa_multiflora Rmu_sc0007404.1_g000005
rosa_roxburghii Rroxscaffold_6G00421740
rosa_rugosa Rorug03G0021300
rosa_samantha Rh3DG085600
rosa_wichuraiana Rw3G007030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1733
AciI CCGC 2 cut(s) 767, 2284
AclWI GGATC 4 cut(s) 94, 877, 1020, 1805
AcsI RAATTY 5 cut(s) 195, 538, 631, 1967, 2197
AcuI CTGAAG 1 cut(s) 693
AcyI GRCGYC 1 cut(s) 1576
AfaI GTAC 3 cut(s) 464, 1046, 1349
AfiI CCNNNNNNNGG 4 cut(s) 963, 1267, 1743, 1774
AflIII ACRYGT 3 cut(s) 432, 459, 1344
AgeI ACCGGT 1 cut(s) 1152
AhdI GACNNNNNGTC 1 cut(s) 285
AjiI CACGTC 2 cut(s) 433, 1661
AjnI CCWGG 5 cut(s) 1008, 1179, 1266, 1418, 1927
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AleI CACNNNNGTG 1 cut(s) 1223
Alw21I GWGCWC 2 cut(s) 853, 1809
Alw26I GTCTC 3 cut(s) 1208, 1388, 2245
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 4 cut(s) 94, 877, 1020, 1805
AoxI GGCC 2 cut(s) 1205, 1270
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 6 cut(s) 131, 998, 1070, 1189, 1256, 2228
ApoI RAATTY 5 cut(s) 195, 538, 631, 1967, 2197
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AsiGI ACCGGT 1 cut(s) 1152
Asp700I GAANNNNTTC 2 cut(s) 989, 2112
AspS9I GGNCC 4 cut(s) 1206, 1270, 1687, 1765
AsuHPI GGTGA 6 cut(s) 1237, 1790, 1877, 2169, 2281, 2282
AsuII TTCGAA 2 cut(s) 629, 1859
AvaII GGWCC 2 cut(s) 1687, 1765
BaeGI GKGCMC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 1733
BanII GRGCYC 1 cut(s) 1809
BbsI GAAGAC 1 cut(s) 1499
Bbv12I GWGCWC 2 cut(s) 853, 1809
BbvI GCAGC 6 cut(s) 118, 1010, 1057, 1201, 1243, 2240
BccI CCATC 4 cut(s) 1817, 1868, 1946, 2182
BciT130I CCWGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
BciVI GTATCC 1 cut(s) 2089
BclI TGATCA 2 cut(s) 453, 598
BcoDI GTCTC 3 cut(s) 1208, 1388, 2245
BfaI CTAG 4 cut(s) 12, 347, 1973, 2021
BfuI GTATCC 1 cut(s) 2089
BisI GCNGC 7 cut(s) 132, 768, 999, 1071, 1190, 1257, 2229
BlpI GCTNAGC 1 cut(s) 1143
BlsI GCNGC 7 cut(s) 133, 769, 1000, 1072, 1191, 1258, 2230
Bme1390I CCNGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
Bme18I GGWCC 2 cut(s) 1687, 1765
BmeRI GACNNNNNGTC 1 cut(s) 285
BmgBI CACGTC 2 cut(s) 433, 1661
BmgT120I GGNCC 4 cut(s) 1206, 1270, 1687, 1765
BmiI GGNNCC 3 cut(s) 1735, 1766, 1829
BmrFI CCNGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
BmrI ACTGGG 1 cut(s) 1751
BmsI GCATC 8 cut(s) 380, 384, 478, 594, 1176, 1587, 1628, 1893
BmuI ACTGGG 1 cut(s) 1751
BpiI GAAGAC 1 cut(s) 1499
Bpu1102I GCTNAGC 1 cut(s) 1143
Bpu14I TTCGAA 2 cut(s) 629, 1859
BpuEI CTTGAG 4 cut(s) 53, 131, 1069, 1658
BsaHI GRCGYC 1 cut(s) 1576
BsaI GGTCTC 1 cut(s) 1208
BsaJI CCNNGG 3 cut(s) 886, 1267, 1927
BsaWI WCCGGW 1 cut(s) 1152
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 4 cut(s) 963, 1267, 1743, 1774
Bse118I RCCGGY 1 cut(s) 1152
Bse1I ACTGG 5 cut(s) 112, 608, 957, 1746, 2142
Bse3DI GCAATG 4 cut(s) 502, 1131, 1452, 2326
BseBI CCWGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
BseDI CCNNGG 3 cut(s) 886, 1267, 1927
BseGI GGATG 6 cut(s) 493, 577, 820, 1111, 1643, 1879
BseLI CCNNNNNNNGG 4 cut(s) 963, 1267, 1743, 1774
BseMI GCAATG 4 cut(s) 502, 1131, 1452, 2326
BseMII CTCAG 6 cut(s) 107, 480, 693, 1089, 1157, 2048
BseNI ACTGG 5 cut(s) 112, 608, 957, 1746, 2142
BseRI GAGGAG 1 cut(s) 1406
BseSI GKGCMC 1 cut(s) 853
BseXI GCAGC 6 cut(s) 118, 1010, 1057, 1201, 1243, 2240
BsgI GTGCAG 1 cut(s) 461
BshFI GGCC 2 cut(s) 1207, 1272
BshNI GGYRCC 1 cut(s) 1733
BshTI ACCGGT 1 cut(s) 1152
BsiHKAI GWGCWC 2 cut(s) 853, 1809
BsiSI CCGG 1 cut(s) 1153
BslFI GGGAC 4 cut(s) 134, 255, 1342, 1778
BslI CCNNNNNNNGG 4 cut(s) 963, 1267, 1743, 1774
BsmAI GTCTC 3 cut(s) 1208, 1388, 2245
BsmBI CGTCTC 1 cut(s) 2245
BsmFI GGGAC 4 cut(s) 134, 255, 1342, 1778
BsmI GAATGC 1 cut(s) 1429
BsnI GGCC 2 cut(s) 1207, 1272
Bso31I GGTCTC 1 cut(s) 1208
Bsp119I TTCGAA 2 cut(s) 629, 1859
Bsp1286I GDGCHC 2 cut(s) 853, 1809
Bsp143I GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
Bsp1720I GCTNAGC 1 cut(s) 1143
Bsp19I CCATGG 1 cut(s) 886
BspACI CCGC 2 cut(s) 767, 2284
BspANI GGCC 2 cut(s) 1207, 1272
BspCNI CTCAG 6 cut(s) 106, 479, 694, 1090, 1156, 2047
BspHI TCATGA 2 cut(s) 1882, 1963
BspLI GGNNCC 3 cut(s) 1735, 1766, 1829
BspPI GGATC 4 cut(s) 94, 877, 1020, 1805
BspT104I TTCGAA 2 cut(s) 629, 1859
BspT107I GGYRCC 1 cut(s) 1733
BspTNI GGTCTC 1 cut(s) 1208
BsrDI GCAATG 4 cut(s) 502, 1131, 1452, 2326
BsrFI RCCGGY 1 cut(s) 1152
BsrI ACTGG 5 cut(s) 112, 608, 957, 1746, 2142
BssAI RCCGGY 1 cut(s) 1152
BssECI CCNNGG 3 cut(s) 886, 1267, 1927
BssMI GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
BssNI GRCGYC 1 cut(s) 1576
BssT1I CCWWGG 1 cut(s) 886
Bst2UI CCWGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
Bst4CI ACNGT 4 cut(s) 269, 990, 2113, 2129
Bst6I CTCTTC 3 cut(s) 169, 2039, 2091
BstACI GRCGYC 1 cut(s) 1576
BstBI TTCGAA 2 cut(s) 629, 1859
BstC8I GCNNGC 4 cut(s) 493, 553, 582, 1243
BstDEI CTNAG 8 cut(s) 93, 466, 585, 702, 1074, 1098, 1143, 2034
BstDSI CCRYGG 1 cut(s) 886
BstF5I GGATG 6 cut(s) 493, 577, 820, 1111, 1643, 1879
BstKTI GATC 8 cut(s) 102, 456, 601, 885, 1015, 1651, 1813, 1944
BstMAI GTCTC 3 cut(s) 1208, 1388, 2245
BstMBI GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
BstMWI GCNNNNNNNGC 2 cut(s) 368, 1804
BstNI CCWGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
BstNSI RCATGY 3 cut(s) 463, 849, 1348
BstSCI CCNGG 5 cut(s) 1008, 1179, 1266, 1418, 1927
BstSLI GKGCMC 1 cut(s) 853
BstV1I GCAGC 6 cut(s) 118, 1010, 1057, 1201, 1243, 2240
BstV2I GAAGAC 1 cut(s) 1499
BsuI GTATCC 1 cut(s) 2089
BsuRI GGCC 2 cut(s) 1207, 1272
BtgI CCRYGG 1 cut(s) 886
BtrI CACGTC 2 cut(s) 433, 1661
BtsCI GGATG 6 cut(s) 493, 577, 820, 1111, 1643, 1879
BtsIMutI CAGTG 5 cut(s) 105, 156, 178, 615, 2134
Cac8I GCNNGC 4 cut(s) 493, 553, 582, 1243
CciI TCATGA 2 cut(s) 1882, 1963
Cfr10I RCCGGY 1 cut(s) 1152
Cfr13I GGNCC 4 cut(s) 1206, 1270, 1687, 1765
CseI GACGC 2 cut(s) 1219, 1565
Csp6I GTAC 3 cut(s) 463, 1045, 1348
CspAI ACCGGT 1 cut(s) 1152
CviQI GTAC 3 cut(s) 463, 1045, 1348
DdeI CTNAG 8 cut(s) 93, 466, 585, 702, 1074, 1098, 1143, 2034
DpnI GATC 8 cut(s) 101, 455, 600, 884, 1014, 1650, 1812, 1943
DpnII GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
DriI GACNNNNNGTC 1 cut(s) 285
Eam1104I CTCTTC 3 cut(s) 169, 2039, 2091
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 3 cut(s) 169, 2039, 2091
Ecl136II GAGCTC 1 cut(s) 1807
Eco130I CCWWGG 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 1809
Eco31I GGTCTC 1 cut(s) 1208
Eco32I GATATC 3 cut(s) 1020, 1038, 1131
Eco47I GGWCC 2 cut(s) 1687, 1765
Eco53kI GAGCTC 1 cut(s) 1807
Eco57I CTGAAG 1 cut(s) 693
EcoICRI GAGCTC 1 cut(s) 1807
EcoO109I RGGNCCY 1 cut(s) 1687
EcoRI GAATTC 2 cut(s) 538, 631
EcoRII CCWGG 5 cut(s) 1008, 1179, 1266, 1418, 1927
EcoRV GATATC 3 cut(s) 1020, 1038, 1131
EcoT14I CCWWGG 1 cut(s) 886
EcoT22I ATGCAT 3 cut(s) 399, 1378, 1643
EcoT38I GRGCYC 1 cut(s) 1809
ErhI CCWWGG 1 cut(s) 886
Esp3I CGTCTC 1 cut(s) 2245
FalI AAGNNNNNCTT 2 cut(s) 2270, 2302
FaqI GGGAC 4 cut(s) 134, 255, 1342, 1778
FauNDI CATATG 2 cut(s) 127, 948
FbaI TGATCA 2 cut(s) 453, 598
Fnu4HI GCNGC 7 cut(s) 132, 768, 999, 1071, 1190, 1257, 2229
FokI GGATG 6 cut(s) 500, 584, 827, 1118, 1650, 1886
FriOI GRGCYC 1 cut(s) 1809
Fsp4HI GCNGC 7 cut(s) 132, 768, 999, 1071, 1190, 1257, 2229
FspBI CTAG 4 cut(s) 12, 347, 1973, 2021
GluI GCNGC 7 cut(s) 132, 768, 999, 1071, 1190, 1257, 2229
HaeIII GGCC 2 cut(s) 1207, 1272
HapII CCGG 1 cut(s) 1153
HgaI GACGC 2 cut(s) 1219, 1565
Hin1I GRCGYC 1 cut(s) 1576
HindIII AAGCTT 3 cut(s) 249, 354, 2247
HinfI GANTC 8 cut(s) 64, 141, 161, 425, 524, 1088, 1279, 1589
HpaII CCGG 1 cut(s) 1153
HphI GGTGA 6 cut(s) 1237, 1790, 1877, 2169, 2281, 2282
Hpy166II GTNNAC 3 cut(s) 851, 868, 2171
Hpy188I TCNGA 8 cut(s) 96, 376, 430, 603, 1017, 1162, 2037, 2123
Hpy188III TCNNGA 4 cut(s) 1883, 1964, 2242, 2258
Hpy8I GTNNAC 3 cut(s) 851, 868, 2171
Hpy99I CGWCG 1 cut(s) 1581
HpyAV CCTTC 4 cut(s) 377, 700, 1096, 2045
HpyCH4III ACNGT 4 cut(s) 269, 990, 2113, 2129
HpyCH4IV ACGT 2 cut(s) 432, 1660
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 1804
HpyF3I CTNAG 8 cut(s) 93, 466, 585, 702, 1074, 1098, 1143, 2034
HpySE526I ACGT 2 cut(s) 432, 1660
Hsp92I GRCGYC 1 cut(s) 1576
Ksp22I TGATCA 2 cut(s) 453, 598
Kzo9I GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
LmnI GCTCC 2 cut(s) 1541, 2306
Lsp1109I GCAGC 6 cut(s) 118, 1010, 1057, 1201, 1243, 2240
LweI GCATC 8 cut(s) 380, 384, 478, 594, 1176, 1587, 1628, 1893
MaeI CTAG 4 cut(s) 12, 347, 1973, 2021
MaeII ACGT 2 cut(s) 432, 1660
MaeIII GTNAC 7 cut(s) 173, 473, 667, 1504, 1550, 2219, 2258
MalI GATC 8 cut(s) 101, 455, 600, 884, 1014, 1650, 1812, 1943
MboI GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
MboII GAAGA 7 cut(s) 156, 920, 1504, 1643, 1803, 2056, 2108
MfeI CAATTG 1 cut(s) 2142
MhlI GDGCHC 2 cut(s) 853, 1809
MlyI GAGTC 3 cut(s) 150, 155, 518
MmeI TCCRAC 1 cut(s) 501
Mph1103I ATGCAT 3 cut(s) 399, 1378, 1643
MroXI GAANNNNTTC 2 cut(s) 989, 2112
MseI TTAA 6 cut(s) 924, 1308, 1314, 1341, 1626, 2298
MslI CAYNNNNRTG 3 cut(s) 242, 1223, 2267
MspA1I CMGCKG 2 cut(s) 863, 1192
MspI CCGG 1 cut(s) 1153
MspR9I CCNGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
MunI CAATTG 1 cut(s) 2142
Mva1269I GAATGC 1 cut(s) 1429
MvaI CCWGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
MwoI GCNNNNNNNGC 2 cut(s) 368, 1804
NcoI CCATGG 1 cut(s) 886
NdeI CATATG 2 cut(s) 127, 948
NdeII GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
NlaIV GGNNCC 3 cut(s) 1735, 1766, 1829
NmeAIII GCCGAG 3 cut(s) 658, 1006, 1217
NmuCI GTSAC 7 cut(s) 173, 473, 667, 1504, 1550, 2219, 2258
NsiI ATGCAT 3 cut(s) 399, 1378, 1643
NspI RCATGY 3 cut(s) 463, 849, 1348
NspV TTCGAA 2 cut(s) 629, 1859
OliI CACNNNNGTG 1 cut(s) 1223
PagI TCATGA 2 cut(s) 1882, 1963
PciI ACATGT 2 cut(s) 459, 1344
PcsI WCGNNNNNNNCGW 2 cut(s) 626, 725
PctI GAATGC 1 cut(s) 1429
PdmI GAANNNNTTC 2 cut(s) 989, 2112
PfeI GAWTC 5 cut(s) 64, 425, 1088, 1279, 1589
PfoI TCCNGGA 1 cut(s) 1179
PinAI ACCGGT 1 cut(s) 1152
PkrI GCNGC 7 cut(s) 133, 769, 1000, 1072, 1191, 1258, 2230
PleI GAGTC 3 cut(s) 149, 155, 518
PpsI GAGTC 3 cut(s) 149, 155, 518
PpuMI RGGWCCY 1 cut(s) 1687
PscI ACATGT 2 cut(s) 459, 1344
Psp124BI GAGCTC 1 cut(s) 1809
Psp5II RGGWCCY 1 cut(s) 1687
Psp6I CCWGG 5 cut(s) 1008, 1179, 1266, 1418, 1927
PspGI CCWGG 5 cut(s) 1008, 1179, 1266, 1418, 1927
PspN4I GGNNCC 3 cut(s) 1735, 1766, 1829
PspPI GGNCC 4 cut(s) 1206, 1270, 1687, 1765
PspPPI RGGWCCY 1 cut(s) 1687
PvuII CAGCTG 2 cut(s) 863, 1192
RsaI GTAC 3 cut(s) 464, 1046, 1349
RsaNI GTAC 3 cut(s) 463, 1045, 1348
RseI CAYNNNNRTG 3 cut(s) 242, 1223, 2267
SacI GAGCTC 1 cut(s) 1809
SaqAI TTAA 6 cut(s) 924, 1308, 1314, 1341, 1626, 2298
SatI GCNGC 7 cut(s) 132, 768, 999, 1071, 1190, 1257, 2229
Sau3AI GATC 8 cut(s) 99, 453, 598, 882, 1012, 1648, 1810, 1941
Sau96I GGNCC 4 cut(s) 1206, 1270, 1687, 1765
SchI GAGTC 3 cut(s) 150, 155, 518
ScrFI CCNGG 5 cut(s) 1010, 1181, 1268, 1420, 1929
SduI GDGCHC 2 cut(s) 853, 1809
SfaNI GCATC 8 cut(s) 380, 384, 478, 594, 1176, 1587, 1628, 1893
SfuI TTCGAA 2 cut(s) 629, 1859
SinI GGWCC 2 cut(s) 1687, 1765
SmiMI CAYNNNNRTG 3 cut(s) 242, 1223, 2267
SmlI CTYRAG 4 cut(s) 68, 146, 1048, 1673
SmoI CTYRAG 4 cut(s) 68, 146, 1048, 1673
SsiI CCGC 2 cut(s) 767, 2284
SspMI CTAG 4 cut(s) 12, 347, 1973, 2021
SstI GAGCTC 1 cut(s) 1809
StyD4I CCNGG 5 cut(s) 1008, 1179, 1266, 1418, 1927
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 4 cut(s) 269, 990, 2113, 2129
TaiI ACGT 2 cut(s) 435, 1663
TatI WGTACW 2 cut(s) 462, 1347
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 5 cut(s) 64, 425, 1088, 1279, 1589
Tru1I TTAA 6 cut(s) 924, 1308, 1314, 1341, 1626, 2298
Tru9I TTAA 6 cut(s) 924, 1308, 1314, 1341, 1626, 2298
TscAI CASTG 5 cut(s) 112, 163, 178, 615, 2134
TseFI GTSAC 7 cut(s) 173, 473, 667, 1504, 1550, 2219, 2258
TseI GCWGC 6 cut(s) 131, 998, 1070, 1189, 1256, 2228
Tsp45I GTSAC 7 cut(s) 173, 473, 667, 1504, 1550, 2219, 2258
TspGWI ACGGA 1 cut(s) 1650
TspRI CASTG 5 cut(s) 112, 163, 178, 615, 2134
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 2 cut(s) 1687, 1765
XapI RAATTY 5 cut(s) 195, 538, 631, 1967, 2197
XceI RCATGY 3 cut(s) 463, 849, 1348
XmnI GAANNNNTTC 2 cut(s) 989, 2112
XspI CTAG 4 cut(s) 12, 347, 1973, 2021
Zsp2I ATGCAT 3 cut(s) 399, 1378, 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.