Rh3DG285500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0009279
Physical Location & Seq
Forward (+)
29137622 .. 29146938
9317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG285500.1

Sequence Viewer

Length: 576 bp
ATGATTTTGCCTTTAGGTTTGGGCTTGCAAGATTTACACAAGGACAGGGACACAACTAATGGTTGGACTGAGGAAGATCCCCTGATCGTAAAGTCCTATTCTGGAGATGATTCTCAAGCTAGGAATTTGGTTCCGTATGCAATTCCAACTCCTATTCCAAGTCTGAGGTTGAAGGAAATGACAGTAGGTCAACTGAAGGAATTGGCAAAGAGGCTCAAAGTGAAAGGCTTTTCCAAGCTTAAGAAAATGGAGCTGCAGGTTGAGCTACTAGCCAGGAAACAGAGAGGTGACTGGTTGTGGCTGCAGGTGCTAAATCAGAAAAGTGCTACTTCACCAAATCTCCCTGCTGTTTCTTCAGTACTGCTTCTAAGGAGCAGTATGGGTATTACTGAAGCATTGCAATTGCAGGTCCAAAAGCGGCTTCATGAACAACTTGAGTGTGACCATTTTTTTTCTGGGATAGATTGGAGTGCAAGGTCAAACAAAATAGTTACTTCATCTCATGATTGGAACTTGAGAAGATTTCCTTGTACCCCCCTTGAAGGGGAAAAAAGTTCGCATCGAATGAATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

191

Amino Acids

21.75

Weight (kDa)

9.42

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 57 - 89 4.3e-06 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 3 cut(s) 247, 295, 397
AciI CCGC 1 cut(s) 418
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 3 cut(s) 215, 339, 411
AfaI GTAC 2 cut(s) 360, 532
AfiI CCNNNNNNNGG 3 cut(s) 542, 543, 544
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 2 cut(s) 172, 542
AhdI GACNNNNNGTC 1 cut(s) 186
AjnI CCWGG 1 cut(s) 272
AjuI GAANNNNNNNTTGG 2 cut(s) 405, 437
AluBI AGCT 4 cut(s) 119, 238, 253, 265
AluI AGCT 4 cut(s) 119, 238, 253, 265
AlwI GGATC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 253, 301
ApoI RAATTY 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 2 cut(s) 299, 324
AvaII GGWCC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 240, 288
BciT130I CCWGG 1 cut(s) 274
BfaI CTAG 2 cut(s) 120, 269
BfmI CTRYAG 2 cut(s) 254, 302
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 3 cut(s) 247, 295, 397
BisI GCNGC 3 cut(s) 254, 302, 419
BlsI GCNGC 3 cut(s) 255, 303, 420
BmcAI AGTACT 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 274
Bme18I GGWCC 1 cut(s) 409
BmeRI GACNNNNNGTC 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 1 cut(s) 568
BpmI CTGGAG 1 cut(s) 123
BpuEI CTTGAG 3 cut(s) 99, 455, 535
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 542, 543, 544
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 274
BseLI CCNNNNNNNGG 3 cut(s) 542, 543, 544
BseMI GCAATG 1 cut(s) 395
BseMII CTCAG 2 cut(s) 60, 155
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 2 cut(s) 240, 288
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 542, 543, 544
BsmFI GGGAC 1 cut(s) 62
Bsp143I GATC 2 cut(s) 76, 84
BspACI CCGC 1 cut(s) 418
BspCNI CTCAG 2 cut(s) 61, 156
BspHI TCATGA 2 cut(s) 424, 502
BspLI GGNNCC 1 cut(s) 132
BspMAI CTGCAG 2 cut(s) 258, 306
BspMI ACCTGC 3 cut(s) 247, 295, 397
BspPI GGATC 1 cut(s) 71
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 395
BsrI ACTGG 1 cut(s) 296
BssMI GATC 2 cut(s) 76, 84
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 69, 164, 368
BstKTI GATC 2 cut(s) 79, 87
BstMBI GATC 2 cut(s) 76, 84
BstMWI GCNNNNNNNGC 2 cut(s) 262, 307
BstNI CCWGG 1 cut(s) 274
BstSCI CCNGG 1 cut(s) 272
BstSFI CTRYAG 2 cut(s) 254, 302
BstV1I GCAGC 2 cut(s) 240, 288
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BveI ACCTGC 3 cut(s) 247, 295, 397
Cac8I GCNNGC 1 cut(s) 26
CciI TCATGA 2 cut(s) 424, 502
Cfr13I GGNCC 1 cut(s) 409
Csp6I GTAC 2 cut(s) 359, 531
CviAII CATG 2 cut(s) 425, 503
CviQI GTAC 2 cut(s) 359, 531
DdeI CTNAG 3 cut(s) 69, 164, 368
DpnI GATC 2 cut(s) 78, 86
DpnII GATC 2 cut(s) 76, 84
DriI GACNNNNNGTC 1 cut(s) 186
Eam1105I GACNNNNNGTC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 3 cut(s) 215, 339, 411
EcoRII CCWGG 1 cut(s) 272
FaeI CATG 2 cut(s) 428, 506
FaiI YATR 4 cut(s) 138, 380, 426, 504
FalI AAGNNNNNCTT 4 cut(s) 313, 345, 511, 543
FaqI GGGAC 1 cut(s) 62
FatI CATG 2 cut(s) 424, 502
Fnu4HI GCNGC 3 cut(s) 254, 302, 419
Fsp4HI GCNGC 3 cut(s) 254, 302, 419
FspBI CTAG 2 cut(s) 120, 269
GluI GCNGC 3 cut(s) 254, 302, 419
GsuI CTGGAG 1 cut(s) 123
Hin1II CATG 2 cut(s) 428, 506
HincII GTYRAC 1 cut(s) 191
HindII GTYRAC 1 cut(s) 191
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 110, 568
HphI GGTGA 2 cut(s) 299, 324
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 3 cut(s) 165, 318, 575
Hpy188III TCNNGA 3 cut(s) 102, 425, 503
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 166, 190, 536
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 7 cut(s) 28, 140, 256, 304, 400, 406, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 307
HpyF3I CTNAG 3 cut(s) 69, 164, 368
Hsp92II CATG 2 cut(s) 428, 506
Kzo9I GATC 2 cut(s) 76, 84
LmnI GCTCC 2 cut(s) 250, 372
Lsp1109I GCAGC 2 cut(s) 240, 288
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 120, 269
MaeIII GTNAC 3 cut(s) 287, 440, 490
MalI GATC 2 cut(s) 78, 86
MboI GATC 2 cut(s) 76, 84
MboII GAAGA 3 cut(s) 86, 345, 531
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 1 cut(s) 76
MluCI AATT 4 cut(s) 124, 141, 200, 401
MmeI TCCRAC 2 cut(s) 44, 170
MnlI CCTC 4 cut(s) 64, 159, 204, 278
MseI TTAA 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 274
MunI CAATTG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 274
MwoI GCNNNNNNNGC 2 cut(s) 262, 307
NdeII GATC 2 cut(s) 76, 84
NlaIII CATG 2 cut(s) 428, 506
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 287, 440
PagI TCATGA 2 cut(s) 424, 502
PaqCI CACCTGC 1 cut(s) 295
PfeI GAWTC 2 cut(s) 110, 568
PkrI GCNGC 3 cut(s) 255, 303, 420
Psp6I CCWGG 1 cut(s) 272
PspGI CCWGG 1 cut(s) 272
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 409
PstI CTGCAG 2 cut(s) 258, 306
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 360, 532
RsaNI GTAC 2 cut(s) 359, 531
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 3 cut(s) 254, 302, 419
Sau3AI GATC 2 cut(s) 76, 84
Sau96I GGNCC 1 cut(s) 409
ScaI AGTACT 1 cut(s) 360
ScrFI CCNGG 1 cut(s) 274
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 2 cut(s) 254, 302
SinI GGWCC 1 cut(s) 409
SmlI CTYRAG 4 cut(s) 114, 239, 434, 514
SmoI CTYRAG 4 cut(s) 114, 239, 434, 514
Sse9I AATT 4 cut(s) 124, 141, 200, 401
SsiI CCGC 1 cut(s) 418
SspMI CTAG 2 cut(s) 120, 269
StyD4I CCNGG 1 cut(s) 272
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 562
TasI AATT 4 cut(s) 124, 141, 200, 401
TatI WGTACW 1 cut(s) 358
TauI GCSGC 1 cut(s) 421
TfiI GAWTC 2 cut(s) 110, 568
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TseFI GTSAC 2 cut(s) 287, 440
TseI GCWGC 2 cut(s) 253, 301
Tsp45I GTSAC 2 cut(s) 287, 440
TspDTI ATGAA 3 cut(s) 413, 441, 486
TspGWI ACGGA 1 cut(s) 123
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XspI CTAG 2 cut(s) 120, 269
ZrmI AGTACT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.