Rh4AG069400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017376
Physical Location & Seq
Reverse (-)
14468558 .. 14471520
2963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG069400.1

Sequence Viewer

Length: 768 bp
ATGAAAACGAAGAAGTACAAAAACATAAACAGAGAAAGAGACCCAGAAGAAGAAGAAGAAGCAGCTGAAACAGAAGGTACAGATGATGAAGTTAGTGTTTTGAGGGACCCACTGGAGCTGTTTGGTTTGGACATAATGTTGAATATACTGAGCAACCTTGACGCACGCAGTGGAGTACTCTCCCTCCTTGTTTGCCATGCTTGGAACGGCGTTGCTTCCAGTGATCGCCTTTGGAGTTCCAAGTGTGAGGAGTTGTGGCTTGGGAAAGCACACATGCCCCGGTTGTTGCAAGTTTGGGGATTGTTGAGGTTGGCCGCTTATTCATTATCTATCGTGGATGGCAAACGTTCACGCATAATGAGGGATGATTTGTGCGACCATGTTTGGGAGTTTCATTTCAACGAGGCCGCTCCTGAATACTGGCGAAGGCTTGATCCTTATTGGAACGGCGCTGGCTCCCTTATGCGCCGCTACTTTCATCCTGATGGGAGCCAAACTCCAGATCATGGTGATCAAGTATGGGGCGGCCATGAGTGCTGTTATTGCACCATTACAAGTCTTCTTGAAAATGGAAAAATAAGGGAACACTATGTAAGGATAAACAGATGGCCACGAATGTTTGTGTCCAGAAAGCAGGATTGGAGCTGGGAAATGTCCAGTCACCTCTTTTGCTACTCGAGCATCCCCGACTCTGATAAAGCAAGCGGGACTGGACCGCTTTTTCCAGTTAGTAGTTCAATAAAGCTTGATTATAAAGAGAGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

255

Amino Acids

29.57

Weight (kDa)

5.62

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 44 - 82 4.4e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017376)

Species Orthologous Gene IDs
malus_domestica MD13G1227700.v1.1 MD13G1227800.v1.1
rosa_chinensis RchiOBHm_Chr4g0396531
rosa_laevigata RLG00000009527
rosa_multiflora Rmu_sc0001781.1_g000010
rosa_rugosa Rorug03G0349300 Rorug03G0349300 Rorug03G0349300
rosa_samantha Rh4AG069400 Rh4BG067200 Rh4CG075000 Rh4DG064400
rosa_wichuraiana Rw4G005580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccB7I CCANNNNNTGG 1 cut(s) 506
AccBSI CCGCTC 1 cut(s) 410
AciI CCGC 6 cut(s) 315, 408, 469, 525, 705, 716
AclI AACGTT 1 cut(s) 346
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 3 cut(s) 312, 526, 608
AdeI CACNNNGTG 1 cut(s) 170
AfaI GTAC 3 cut(s) 17, 79, 177
AfiI CCNNNNNNNGG 2 cut(s) 385, 506
AgsI TTSAA 4 cut(s) 142, 400, 566, 738
AjuI GAANNNNNNNTTGG 2 cut(s) 622, 654
AluBI AGCT 4 cut(s) 65, 118, 645, 745
AluI AGCT 4 cut(s) 65, 118, 645, 745
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 4 cut(s) 312, 405, 526, 608
ApeKI GCWGC 1 cut(s) 62
AspLEI GCGC 2 cut(s) 452, 468
AspS9I GGNCC 2 cut(s) 106, 713
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 521, 653
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 2 cut(s) 106, 713
BalI TGGCCA 1 cut(s) 610
BbsI GAAGAC 1 cut(s) 551
BbvI GCAGC 1 cut(s) 74
BccI CCATC 3 cut(s) 332, 479, 600
BceAI ACGGC 2 cut(s) 223, 463
BclI TGATCA 1 cut(s) 511
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 33
BfoI RGCGCY 1 cut(s) 453
BisI GCNGC 5 cut(s) 63, 315, 408, 469, 526
BlsI GCNGC 5 cut(s) 64, 316, 409, 470, 527
BmcAI AGTACT 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 2 cut(s) 106, 713
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 2 cut(s) 106, 713
BmiI GGNNCC 4 cut(s) 107, 108, 457, 491
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 551
BplI GAGNNNNNCTC 2 cut(s) 481, 513
BpmI CTGGAG 2 cut(s) 134, 483
BpuMI CCSGG 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 506
Bse1I ACTGG 6 cut(s) 117, 219, 425, 657, 715, 725
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 4 cut(s) 343, 370, 478, 681
BseLI CCNNNNNNNGG 2 cut(s) 385, 506
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 6 cut(s) 117, 219, 425, 657, 715, 725
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 74
BseYI CCCAGC 1 cut(s) 645
BshFI GGCC 4 cut(s) 314, 407, 528, 610
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 1 cut(s) 280
BslFI GGGAC 2 cut(s) 119, 721
BslI CCNNNNNNNGG 2 cut(s) 385, 506
BsmAI GTCTC 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 119, 721
BsnI GGCC 4 cut(s) 314, 407, 528, 610
Bso31I GGTCTC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 676
Bsp143I GATC 4 cut(s) 223, 433, 502, 511
BspACI CCGC 6 cut(s) 315, 408, 469, 525, 705, 716
BspANI GGCC 4 cut(s) 314, 407, 528, 610
BspCNI CTCAG 1 cut(s) 141
BspLI GGNNCC 4 cut(s) 107, 108, 457, 491
BspPI GGATC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 33
BsrBI CCGCTC 1 cut(s) 410
BsrI ACTGG 6 cut(s) 117, 219, 425, 657, 715, 725
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 4 cut(s) 223, 433, 502, 511
BstC8I GCNNGC 3 cut(s) 166, 454, 703
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 4 cut(s) 343, 370, 478, 681
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 452, 468
BstKTI GATC 4 cut(s) 226, 436, 505, 514
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 4 cut(s) 223, 433, 502, 511
BstMWI GCNNNNNNNGC 3 cut(s) 534, 543, 678
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 551
BsuRI GGCC 4 cut(s) 314, 407, 528, 610
BtsCI GGATG 4 cut(s) 343, 370, 478, 681
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 3 cut(s) 110, 175, 226
Cac8I GCNNGC 3 cut(s) 166, 454, 703
CfoI GCGC 2 cut(s) 452, 468
Cfr13I GGNCC 2 cut(s) 106, 713
CseI GACGC 1 cut(s) 170
Csp6I GTAC 3 cut(s) 16, 78, 176
CviAII CATG 5 cut(s) 197, 274, 380, 506, 530
CviQI GTAC 3 cut(s) 16, 78, 176
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 225, 435, 504, 513
DpnII GATC 4 cut(s) 223, 433, 502, 511
DraIII CACNNNGTG 1 cut(s) 170
EaeI YGGCCR 3 cut(s) 312, 526, 608
Eco31I GGTCTC 1 cut(s) 33
Eco47I GGWCC 2 cut(s) 106, 713
Eco88I CYCGRG 1 cut(s) 676
EcoO109I RGGNCCY 1 cut(s) 106
FaeI CATG 5 cut(s) 200, 277, 383, 509, 533
FaqI GGGAC 2 cut(s) 119, 721
FatI CATG 5 cut(s) 196, 273, 379, 505, 529
FauI CCCGC 1 cut(s) 698
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 5 cut(s) 63, 315, 408, 469, 526
FokI GGATG 4 cut(s) 350, 377, 465, 668
Fsp4HI GCNGC 5 cut(s) 63, 315, 408, 469, 526
GlaI GCGC 2 cut(s) 451, 467
GluI GCNGC 5 cut(s) 63, 315, 408, 469, 526
GsaI CCCAGC 1 cut(s) 649
GsuI CTGGAG 2 cut(s) 134, 483
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 4 cut(s) 314, 407, 528, 610
HapII CCGG 1 cut(s) 280
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 452, 468
Hin1II CATG 5 cut(s) 200, 277, 383, 509, 533
Hin6I GCGC 2 cut(s) 450, 466
HinP1I GCGC 2 cut(s) 450, 466
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 1 cut(s) 689
HpaII CCGG 1 cut(s) 280
HphI GGTGA 2 cut(s) 521, 653
Hpy166II GTNNAC 1 cut(s) 350
Hpy188I TCNGA 1 cut(s) 694
Hpy188III TCNNGA 5 cut(s) 413, 482, 500, 563, 627
Hpy8I GTNNAC 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 68, 420
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 2 cut(s) 289, 546
HpyF10VI GCNNNNNNNGC 3 cut(s) 534, 543, 678
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 1 cut(s) 346
Hsp92II CATG 5 cut(s) 200, 277, 383, 509, 533
HspAI GCGC 2 cut(s) 450, 466
KflI GGGWCCC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 4 cut(s) 223, 433, 502, 511
LmnI GCTCC 5 cut(s) 115, 415, 461, 489, 642
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 1 cut(s) 690
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 659
MalI GATC 4 cut(s) 225, 435, 504, 513
MbiI CCGCTC 1 cut(s) 410
MboI GATC 4 cut(s) 223, 433, 502, 511
MboII GAAGA 6 cut(s) 22, 59, 62, 65, 68, 551
MlsI TGGCCA 1 cut(s) 610
MluNI TGGCCA 1 cut(s) 610
MlyI GAGTC 1 cut(s) 683
MnlI CCTC 7 cut(s) 96, 194, 241, 300, 354, 397, 674
Mox20I TGGCCA 1 cut(s) 610
MscI TGGCCA 1 cut(s) 610
MslI CAYNNNNRTG 1 cut(s) 483
Msp20I TGGCCA 1 cut(s) 610
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 280
MwoI GCNNNNNNNGC 3 cut(s) 534, 543, 678
NciI CCSGG 1 cut(s) 280
NdeII GATC 4 cut(s) 223, 433, 502, 511
NlaIII CATG 5 cut(s) 200, 277, 383, 509, 533
NlaIV GGNNCC 4 cut(s) 107, 108, 457, 491
NmuCI GTSAC 1 cut(s) 659
NspI RCATGY 1 cut(s) 277
PaeR7I CTCGAG 1 cut(s) 676
PflMI CCANNNNNTGG 1 cut(s) 506
PkrI GCNGC 5 cut(s) 64, 316, 409, 470, 527
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
PpuMI RGGWCCY 1 cut(s) 106
PsiI TTATAA 1 cut(s) 753
Psp1406I AACGTT 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 106
PspFI CCCAGC 1 cut(s) 645
PspN4I GGNNCC 4 cut(s) 107, 108, 457, 491
PspPI GGNCC 2 cut(s) 106, 713
PspPPI RGGWCCY 1 cut(s) 106
PspXI VCTCGAGB 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 3 cut(s) 17, 79, 177
RsaNI GTAC 3 cut(s) 16, 78, 176
RseI CAYNNNNRTG 1 cut(s) 483
SatI GCNGC 5 cut(s) 63, 315, 408, 469, 526
Sau3AI GATC 4 cut(s) 223, 433, 502, 511
Sau96I GGNCC 2 cut(s) 106, 713
ScaI AGTACT 1 cut(s) 177
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 280
SetI ASST 9 cut(s) 67, 79, 120, 159, 311, 349, 647, 666, 747
SfaNI GCATC 1 cut(s) 690
Sfr274I CTCGAG 1 cut(s) 676
SinI GGWCC 2 cut(s) 106, 713
SlaI CTCGAG 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
SsiI CCGC 6 cut(s) 315, 408, 469, 525, 705, 716
StyD4I CCNGG 1 cut(s) 278
TaiI ACGT 1 cut(s) 349
TaqI TCGA 1 cut(s) 677
TatI WGTACW 2 cut(s) 15, 175
TauI GCSGC 4 cut(s) 317, 410, 471, 528
TscAI CASTG 3 cut(s) 117, 175, 226
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 5 cut(s) 17, 102, 312, 383, 467
TspRI CASTG 3 cut(s) 117, 175, 226
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 106, 713
XceI RCATGY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 676
ZrmI AGTACT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.