Rw4G005580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017376
Physical Location & Seq
Reverse (-)
11294267 .. 11296897
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005580.1

Sequence Viewer

Length: 549 bp
ATGAAAATGAAGAAGTACAAAAACATAAACAGAGAAAGAGACCCAGAAGAAGAAGAAGAACTGTTTGGTTTGGACATAATGTTGAATATACTGAGCAACCTTGACGCACGCAGTGGAGTACTCTCCCTCCTTGTTTGCCATGCTTGGAACGGCGTTGCTTCCAGTGATCGCCTTTGGAGTTCCAAGTGTGAGGAGTTGTGGCTTGGGAAAGCACACATGCCCCGGTTGTTGCAAGTTTGGGGATTGTTGAGGTTGGCCGCTTATTCATTATCTATCGTGGATGGCAAACATTCACGCATAATGAGGGATGATTTGTGCGACCATGTTTGGGAGTTTCATTTCAACGAGTGCTGTTATTGCACCATTACAAGTCTTCTTGAAAATGGAAAAATAAGGGAACACTATGTAAGGATAAACAGATGGCCACGAATGTTTGTGTCCAGAAAGCAGGATTGGAGCTGGGAAATGTCCAGTCACCTCTTTTGCTACTCGAGCATCCCCGACTCTGATAAAGCAAGCGGGACTGGACCGCTTTTTCCAGTTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

182

Amino Acids

21.34

Weight (kDa)

7.04

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 25 - 63 2.6e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017376)

Species Orthologous Gene IDs
malus_domestica MD13G1227700.v1.1 MD13G1227800.v1.1
rosa_chinensis RchiOBHm_Chr4g0396531
rosa_laevigata RLG00000009527
rosa_multiflora Rmu_sc0001781.1_g000010
rosa_rugosa Rorug03G0349300 Rorug03G0349300 Rorug03G0349300
rosa_samantha Rh4AG069400 Rh4BG067200 Rh4CG075000 Rh4DG064400
rosa_wichuraiana Rw4G005580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 258, 519, 530
AcoI YGGCCR 2 cut(s) 255, 422
AdeI CACNNNGTG 1 cut(s) 113
AfaI GTAC 2 cut(s) 17, 120
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 3 cut(s) 85, 343, 380
AjuI GAANNNNNNNTTGG 4 cut(s) 48, 80, 436, 468
AluBI AGCT 1 cut(s) 459
AluI AGCT 1 cut(s) 459
Alw26I GTCTC 1 cut(s) 33
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 2 cut(s) 255, 422
AspS9I GGNCC 1 cut(s) 527
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 1 cut(s) 527
BalI TGGCCA 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 365
BccI CCATC 2 cut(s) 275, 414
BceAI ACGGC 1 cut(s) 166
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 33
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 527
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 527
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 221
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 4 cut(s) 162, 471, 529, 539
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 3 cut(s) 286, 313, 495
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 4 cut(s) 162, 471, 529, 539
BseRI GAGGAG 1 cut(s) 206
BseYI CCCAGC 1 cut(s) 459
BshFI GGCC 2 cut(s) 257, 424
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 2 cut(s) 257, 424
Bso31I GGTCTC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 490
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 3 cut(s) 258, 519, 530
BspANI GGCC 2 cut(s) 257, 424
BspCNI CTCAG 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 33
BsrI ACTGG 4 cut(s) 162, 471, 529, 539
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 1 cut(s) 166
Bst4CI ACNGT 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 109, 517
BstDEI CTNAG 1 cut(s) 92
BstF5I GGATG 3 cut(s) 286, 313, 495
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 2 cut(s) 357, 492
BstNSI RCATGY 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 221
BstV2I GAAGAC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 257, 424
BtsCI GGATG 3 cut(s) 286, 313, 495
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 2 cut(s) 118, 169
Cac8I GCNNGC 2 cut(s) 109, 517
Cfr13I GGNCC 1 cut(s) 527
CseI GACGC 1 cut(s) 113
Csp6I GTAC 2 cut(s) 16, 119
CviAII CATG 3 cut(s) 140, 217, 323
CviJI RGCY 4 cut(s) 202, 257, 424, 459
CviKI_1 RGCY 4 cut(s) 202, 257, 424, 459
CviQI GTAC 2 cut(s) 16, 119
DdeI CTNAG 1 cut(s) 92
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
DraIII CACNNNGTG 1 cut(s) 113
EaeI YGGCCR 2 cut(s) 255, 422
Eco31I GGTCTC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 490
FaeI CATG 3 cut(s) 143, 220, 326
FaiI YATR 8 cut(s) 26, 77, 89, 141, 218, 299, 324, 405
FaqI GGGAC 1 cut(s) 535
FatI CATG 3 cut(s) 139, 216, 322
FauI CCCGC 1 cut(s) 512
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 3 cut(s) 293, 320, 482
Fsp4HI GCNGC 1 cut(s) 258
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 1 cut(s) 463
HaeIII GGCC 2 cut(s) 257, 424
HapII CCGG 1 cut(s) 223
HgaI GACGC 1 cut(s) 113
Hin1II CATG 3 cut(s) 143, 220, 326
HinfI GANTC 1 cut(s) 503
HpaII CCGG 1 cut(s) 223
HphI GGTGA 1 cut(s) 467
Hpy188I TCNGA 1 cut(s) 508
Hpy188III TCNNGA 2 cut(s) 377, 441
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4V TGCA 2 cut(s) 232, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 357, 492
HpyF3I CTNAG 1 cut(s) 92
Hsp92II CATG 3 cut(s) 143, 220, 326
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 1 cut(s) 456
LpnPI CCDG 8 cut(s) 57, 175, 236, 434, 445, 454, 484, 510
LweI GCATC 1 cut(s) 504
MaeIII GTNAC 1 cut(s) 473
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 6 cut(s) 22, 59, 62, 65, 68, 365
MlsI TGGCCA 1 cut(s) 424
MluNI TGGCCA 1 cut(s) 424
MlyI GAGTC 1 cut(s) 497
MnlI CCTC 5 cut(s) 137, 184, 243, 297, 488
Mox20I TGGCCA 1 cut(s) 424
MscI TGGCCA 1 cut(s) 424
Msp20I TGGCCA 1 cut(s) 424
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 1 cut(s) 223
MwoI GCNNNNNNNGC 2 cut(s) 357, 492
NciI CCSGG 1 cut(s) 223
NdeII GATC 1 cut(s) 166
NlaIII CATG 3 cut(s) 143, 220, 326
NmuCI GTSAC 1 cut(s) 473
NspI RCATGY 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 490
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 459
PspPI GGNCC 1 cut(s) 527
PspXI VCTCGAGB 1 cut(s) 490
RsaI GTAC 2 cut(s) 17, 120
RsaNI GTAC 2 cut(s) 16, 119
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 1 cut(s) 527
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 223
SetI ASST 5 cut(s) 102, 254, 461, 480, 548
SfaNI GCATC 1 cut(s) 504
Sfr274I CTCGAG 1 cut(s) 490
SinI GGWCC 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 490
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
SsiI CCGC 3 cut(s) 258, 519, 530
StyD4I CCNGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 491
TatI WGTACW 2 cut(s) 15, 118
TauI GCSGC 1 cut(s) 260
TscAI CASTG 2 cut(s) 118, 169
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 17, 23, 255, 326
TspRI CASTG 2 cut(s) 118, 169
VpaK11BI GGWCC 1 cut(s) 527
XceI RCATGY 1 cut(s) 220
XhoI CTCGAG 1 cut(s) 490
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.