Rh4BG153400

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017695
Physical Location & Seq
Reverse (-)
27647486 .. 27652513
5028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG153400.1

Sequence Viewer

Length: 330 bp
ATGCGCAGCTGGTTTACCTTATTTGCAGAGAATCTGCAGCAGACCATCTTCTCCAACCATTCCAAGGCCTTCTCATCACTACTTTCAACAATTGCTTTTGAGCCCTACAAGGTCAGCAAAGTGGATCTTCTTGCCCAAAGTTGCTCTTTATGGCTCTTAATTTGGGGGTGCTCGGCACTTGGTGTCTGGAAGCTTAACACATTGGGGTTCCTTCCTACACATGCATCCGATTGGGTTTCATCCTTACCCCCTGCTCAGGTTAAGAGCAAGCTTAAAGGTCACTCTAAAAGGATAACTGGCCTAGCATTCTCTCATGTATGTAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1077

Protein Analysis

109

Amino Acids

12.16

Weight (kDa)

9.56

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC4 PF06417 28 - 82 1.5e-07 ER membrane protein complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017695)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29170
rosa_chinensis RchiOBHm_Chr4g0409071
rosa_multiflora Rmu_sc0004836.1_g000006
rosa_rugosa Rorug02G0304200 Rorug05G0382100 Rorug05G0582100
rosa_samantha Rh2DG044500 Rh4BG153400 Rh5CG359300 Rh6AG188900 Rh6AG189900
rosa_wichuraiana Rw1G012470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AclWI GGATC 1 cut(s) 132
AdeI CACNNNGTG 1 cut(s) 182
AfiI CCNNNNNNNGG 2 cut(s) 64, 256
AgsI TTSAA 1 cut(s) 87
AluBI AGCT 3 cut(s) 9, 193, 271
AluI AGCT 3 cut(s) 9, 193, 271
Alw21I GWGCWC 1 cut(s) 173
AlwI GGATC 1 cut(s) 132
AoxI GGCC 2 cut(s) 66, 298
ApeKI GCWGC 2 cut(s) 6, 37
AspLEI GCGC 1 cut(s) 6
BanII GRGCYC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 18, 49
BccI CCATC 1 cut(s) 53
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 35
BisI GCNGC 2 cut(s) 7, 38
BlsI GCNGC 2 cut(s) 8, 39
BmiI GGNNCC 1 cut(s) 209
BmsI GCATC 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 63
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 256
Bse1I ACTGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 2 cut(s) 224, 239
BseLI CCNNNNNNNGG 2 cut(s) 64, 256
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 18, 49
BshFI GGCC 2 cut(s) 68, 300
BsiHKAI GWGCWC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 64, 256
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 2 cut(s) 68, 300
Bsp1286I GDGCHC 2 cut(s) 105, 173
Bsp143I GATC 1 cut(s) 124
BspANI GGCC 2 cut(s) 68, 300
BspCNI CTCAG 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 209
BspMAI CTGCAG 1 cut(s) 39
BspPI GGATC 1 cut(s) 132
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 269
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 224, 239
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 127
BstMBI GATC 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 18, 49
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 2 cut(s) 68, 300
BtsCI GGATG 2 cut(s) 224, 239
Cac8I GCNNGC 1 cut(s) 269
CfoI GCGC 1 cut(s) 6
CviAII CATG 2 cut(s) 221, 314
CviJI RGCY 7 cut(s) 9, 68, 103, 154, 193, 271, 300
CviKI_1 RGCY 7 cut(s) 9, 68, 103, 154, 193, 271, 300
DdeI CTNAG 1 cut(s) 255
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
DraIII CACNNNGTG 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 2 cut(s) 224, 317
FaiI YATR 4 cut(s) 151, 222, 315, 319
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 130, 162
FatI CATG 2 cut(s) 220, 313
Fnu4HI GCNGC 2 cut(s) 7, 38
FokI GGATG 2 cut(s) 211, 226
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 7, 38
FspBI CTAG 1 cut(s) 302
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GluI GCNGC 2 cut(s) 7, 38
HaeIII GGCC 2 cut(s) 68, 300
HhaI GCGC 1 cut(s) 6
Hin1II CATG 2 cut(s) 224, 317
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HindIII AAGCTT 2 cut(s) 191, 269
HinfI GANTC 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 1 cut(s) 187
Hpy8I GTNNAC 1 cut(s) 15
HpyAV CCTTC 2 cut(s) 79, 221
HpyCH4V TGCA 3 cut(s) 26, 37, 224
HpyF3I CTNAG 1 cut(s) 255
Hsp92II CATG 2 cut(s) 224, 317
HspAI GCGC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 4 cut(s) 172, 242, 264, 282
Lsp1109I GCAGC 2 cut(s) 18, 49
LweI GCATC 1 cut(s) 233
MaeI CTAG 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 278
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 2 cut(s) 40, 119
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 2 cut(s) 105, 173
MluCI AATT 2 cut(s) 90, 159
MmeI TCCRAC 1 cut(s) 78
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 4 cut(s) 158, 195, 261, 273
MspA1I CMGCKG 1 cut(s) 9
MunI CAATTG 1 cut(s) 90
Mva1269I GAATGC 1 cut(s) 305
NdeII GATC 1 cut(s) 124
NlaIII CATG 2 cut(s) 224, 317
NlaIV GGNNCC 1 cut(s) 209
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 278
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 1 cut(s) 224
PceI AGGCCT 1 cut(s) 68
PctI GAATGC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 2 cut(s) 8, 39
PspN4I GGNNCC 1 cut(s) 209
PstI CTGCAG 1 cut(s) 39
PsuI RGATCY 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 9
SaqAI TTAA 4 cut(s) 158, 195, 261, 273
SatI GCNGC 2 cut(s) 7, 38
Sau3AI GATC 1 cut(s) 124
SduI GDGCHC 2 cut(s) 105, 173
SetI ASST 7 cut(s) 11, 20, 114, 195, 261, 273, 280
SfaNI GCATC 1 cut(s) 233
SfcI CTRYAG 1 cut(s) 35
Sse9I AATT 2 cut(s) 90, 159
SseBI AGGCCT 1 cut(s) 68
SspMI CTAG 1 cut(s) 302
StuI AGGCCT 1 cut(s) 68
StyI CCWWGG 1 cut(s) 63
TasI AATT 2 cut(s) 90, 159
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 4 cut(s) 158, 195, 261, 273
Tru9I TTAA 4 cut(s) 158, 195, 261, 273
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 2 cut(s) 6, 37
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 228
XceI RCATGY 1 cut(s) 224
XspI CTAG 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.