Rh4BG437400

Pentatricopeptide repeat-containing protein At5g13770

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008792
Physical Location & Seq
Reverse (-)
57816969 .. 57817931
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG437400.1

Sequence Viewer

Length: 963 bp
ATGTCAAAGGAAGACATTTCTTTAGACTCTTCAAAGATATATTCTGTTTTGATCAGTTCATTTGCGAGCATTCGAGAAGTGAAGGTAGCTGAAGAGCTGTTTGAAGAAGCAGAAACCAAGAAAATGTTGAGGAATCCAACTGTATTTTTGAGACTGATCACAATGTATGTTGAAGAAGGGTTGGTGGATAAGACTCTTGAAATAATCAAGGCAATGAAGGGTGCAAATATCAGAGTTTCCGATAACATATGCTGCGCAGTTGTGAGTGGCTTCTCCAGGAAAAGCAGGTTTCTCACAGCAGTTAAAGTGTTTGAAGATTTGATCTCAATGGGATGTGAACCGGGACAGGTGACATACGCTTCGATTATCAATGTGTATTGTCGTCTTGGGCTTCATTCTAAGGCTGAGATGGTGTTTAGAGAGATGGAAGAAAAAGGGTTTGATAAATGTGTTGTGGCGTATTCAAGCATGGTTGTAATGTATGGGAAGACAGGAAGGCCAAGAGAGGCAATGAGGCTTGTGGCCAAGATGAAAGAAAGAGGGTGTAAGCCGAATGTGTGGATTTACAATTCTTTGATGGATATGCATGGAAGGGATAATAATACGAGGCAAGTTGCAAAGCTATGGAGCGAAATGAAGCGAAGGAAGGTAGCACCAGATAAGGTGAGCTACACAACTGTTATAGGTGCTTATAACAAGGCAGGGGACTTTGATATGTGCTTGAGATTTTACAATGAGTATAGGACTCATGGCGGTGTTTTGGACAAGGCTATGGCTGGAATGATGGTTGGTGTTTTCTCCAAGAGTGGTCATGTTGACGAGTTGGTGAAGCTTCTGCGAGATATGAAAGCAGAAGGAACAGGGTTAGATGTGAGGCTTTATCGGTCCGCCTTGAATGCTTTGGCAGATTCTGGTCTGGAAATGCAGGTGAAATGGTTGCAAAGTAGCTTTGAGATGACATAG

Protein Analysis

320

Amino Acids

36.2

Weight (kDa)

9.0

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 86 - 128 1.4e-06 PPR repeat family
PPR_1 PF12854 110 - 143 6.7e-09 PPR repeat
PPR_2 PF13041 114 - 147 1.5e-06 PPR repeat family
PPR PF01535 117 - 147 1.5e-08 PPR repeat
PPR_3 PF13812 138 - 197 1.6e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 150 - 186 1.2e-08 PPR repeat family
PPR PF01535 152 - 182 1.2e-06 PPR repeat
PPR_long PF17177 168 - 279 6.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 184 - 230 5.2e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AarI CACCTGC 1 cut(s) 916
Acc16I TGCGCA 1 cut(s) 256
Acc36I ACCTGC 2 cut(s) 276, 916
AciI CCGC 2 cut(s) 753, 888
AcoI YGGCCR 1 cut(s) 522
AcuI CTGAAG 1 cut(s) 111
AgsI TTSAA 7 cut(s) 33, 104, 173, 200, 314, 465, 895
AjnI CCWGG 1 cut(s) 275
AluBI AGCT 6 cut(s) 89, 97, 622, 669, 832, 948
AluI AGCT 6 cut(s) 89, 97, 622, 669, 832, 948
Alw26I GTCTC 1 cut(s) 145
AlwNI CAGNNNCTG 1 cut(s) 911
AoxI GGCC 2 cut(s) 497, 522
ApeKI GCWGC 1 cut(s) 252
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 885
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 4 cut(s) 361, 676, 838, 940
AvaII GGWCC 1 cut(s) 885
BalI TGGCCA 1 cut(s) 524
BbsI GAAGAC 2 cut(s) 18, 494
BbvI GCAGC 1 cut(s) 239
BccI CCATC 4 cut(s) 403, 418, 571, 778
BciT130I CCWGG 1 cut(s) 277
BclI TGATCA 2 cut(s) 51, 156
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 145
BfuAI ACCTGC 2 cut(s) 276, 916
BisI GCNGC 1 cut(s) 253
BlsI GCNGC 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 277, 342
Bme18I GGWCC 1 cut(s) 885
BmgT120I GGNCC 1 cut(s) 885
BmrFI CCNGG 2 cut(s) 277, 342
BpiI GAAGAC 2 cut(s) 18, 494
BpmI CTGGAG 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 742
BpuMI CCSGG 1 cut(s) 342
Bse3DI GCAATG 2 cut(s) 219, 516
BseBI CCWGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 338
BseMI GCAATG 2 cut(s) 219, 516
BseMII CTCAG 1 cut(s) 396
BseXI GCAGC 1 cut(s) 239
BshFI GGCC 2 cut(s) 499, 524
BsiSI CCGG 1 cut(s) 341
BslFI GGGAC 2 cut(s) 357, 719
BsmAI GTCTC 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 357, 719
BsmI GAATGC 2 cut(s) 69, 901
BsnI GGCC 2 cut(s) 499, 524
Bsp143I GATC 3 cut(s) 51, 156, 321
BspACI CCGC 2 cut(s) 753, 888
BspANI GGCC 2 cut(s) 499, 524
BspCNI CTCAG 1 cut(s) 397
BspMI ACCTGC 2 cut(s) 276, 916
BspQI GCTCTTC 1 cut(s) 87
BsrDI GCAATG 2 cut(s) 219, 516
BssMI GATC 3 cut(s) 51, 156, 321
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 2 cut(s) 142, 679
Bst6I CTCTTC 2 cut(s) 34, 87
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 399, 405
BstF5I GGATG 1 cut(s) 338
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 3 cut(s) 54, 159, 324
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 3 cut(s) 51, 156, 321
BstMWI GCNNNNNNNGC 1 cut(s) 896
BstNI CCWGG 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 275, 340
BstV1I GCAGC 1 cut(s) 239
BstV2I GAAGAC 2 cut(s) 18, 494
BsuRI GGCC 2 cut(s) 499, 524
BtsCI GGATG 1 cut(s) 338
BveI ACCTGC 2 cut(s) 276, 916
Cac8I GCNNGC 1 cut(s) 67
CaiI CAGNNNCTG 1 cut(s) 911
CfoI GCGC 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 885
CpoI CGGWCCG 1 cut(s) 885
CspI CGGWCCG 1 cut(s) 885
CviAII CATG 4 cut(s) 469, 587, 749, 812
DdeI CTNAG 2 cut(s) 399, 405
DpnI GATC 3 cut(s) 53, 158, 323
DpnII GATC 3 cut(s) 51, 156, 321
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 2 cut(s) 34, 87
EarI CTCTTC 2 cut(s) 34, 87
EciI GGCGGA 1 cut(s) 877
Eco47I GGWCC 1 cut(s) 885
Eco57I CTGAAG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 588
FaeI CATG 4 cut(s) 472, 590, 752, 815
FaqI GGGAC 2 cut(s) 357, 719
FatI CATG 4 cut(s) 468, 586, 748, 811
FauNDI CATATG 1 cut(s) 248
FbaI TGATCA 2 cut(s) 51, 156
Fnu4HI GCNGC 1 cut(s) 253
FokI GGATG 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 253
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 1 cut(s) 256
GluI GCNGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 259
HaeIII GGCC 2 cut(s) 499, 524
HapII CCGG 1 cut(s) 341
HhaI GCGC 1 cut(s) 257
Hin1II CATG 4 cut(s) 472, 590, 752, 815
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 5 cut(s) 26, 133, 193, 745, 908
HpaII CCGG 1 cut(s) 341
HphI GGTGA 4 cut(s) 361, 676, 838, 940
Hpy166II GTNNAC 2 cut(s) 338, 817
Hpy188I TCNGA 2 cut(s) 233, 241
Hpy188III TCNNGA 3 cut(s) 74, 197, 917
Hpy8I GTNNAC 2 cut(s) 338, 817
HpyAV CCTTC 8 cut(s) 76, 170, 211, 489, 585, 636, 640, 848
HpyCH4III ACNGT 2 cut(s) 142, 679
HpyCH4V TGCA 5 cut(s) 224, 586, 617, 925, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 896
HpyF3I CTNAG 2 cut(s) 399, 405
Hsp92II CATG 4 cut(s) 472, 590, 752, 815
HspAI GCGC 1 cut(s) 255
Ksp22I TGATCA 2 cut(s) 51, 156
Kzo9I GATC 3 cut(s) 51, 156, 321
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 627
Lsp1109I GCAGC 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 349
MalI GATC 3 cut(s) 53, 158, 323
MboI GATC 3 cut(s) 51, 156, 321
MboII GAAGA 8 cut(s) 21, 23, 104, 116, 185, 326, 440, 499
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 1 cut(s) 568
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 3 cut(s) 20, 187, 739
MmeI TCCRAC 1 cut(s) 161
MnlI CCTC 6 cut(s) 123, 499, 507, 533, 600, 867
Mox20I TGGCCA 1 cut(s) 524
Mph1103I ATGCAT 1 cut(s) 588
MscI TGGCCA 1 cut(s) 524
MseI TTAA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 753
Msp20I TGGCCA 1 cut(s) 524
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 2 cut(s) 277, 342
Mva1269I GAATGC 2 cut(s) 69, 901
MvaI CCWGG 1 cut(s) 277
MwoI GCNNNNNNNGC 1 cut(s) 896
NciI CCSGG 1 cut(s) 342
NdeI CATATG 1 cut(s) 248
NdeII GATC 3 cut(s) 51, 156, 321
NlaIII CATG 4 cut(s) 472, 590, 752, 815
NmuCI GTSAC 1 cut(s) 349
NsbI TGCGCA 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 588
PaqCI CACCTGC 1 cut(s) 916
PciSI GCTCTTC 1 cut(s) 87
PctI GAATGC 2 cut(s) 69, 901
PfeI GAWTC 2 cut(s) 133, 908
PfoI TCCNGGA 1 cut(s) 275
PkrI GCNGC 1 cut(s) 254
PleI GAGTC 3 cut(s) 20, 187, 739
PpsI GAGTC 3 cut(s) 20, 187, 739
PsiI TTATAA 1 cut(s) 693
Psp6I CCWGG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 275
PspPI GGNCC 1 cut(s) 885
PstNI CAGNNNCTG 1 cut(s) 911
RseI CAYNNNNRTG 1 cut(s) 753
Rsr2I CGGWCCG 1 cut(s) 885
RsrII CGGWCCG 1 cut(s) 885
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 1 cut(s) 253
Sau3AI GATC 3 cut(s) 51, 156, 321
Sau96I GGNCC 1 cut(s) 885
SchI GAGTC 3 cut(s) 20, 187, 739
ScrFI CCNGG 2 cut(s) 277, 342
SinI GGWCC 1 cut(s) 885
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 721
SmoI CTYRAG 1 cut(s) 721
Sse9I AATT 1 cut(s) 568
SsiI CCGC 2 cut(s) 753, 888
StyD4I CCNGG 2 cut(s) 275, 340
TaaI ACNGT 2 cut(s) 142, 679
TaqI TCGA 2 cut(s) 73, 362
TaqII GACCGA 1 cut(s) 873
TasI AATT 1 cut(s) 568
TfiI GAWTC 2 cut(s) 133, 908
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 1 cut(s) 252
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 6 cut(s) 48, 230, 383, 545, 650, 860
VpaK11BI GGWCC 1 cut(s) 885
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.