Rh4CG056100

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012680
Physical Location & Seq
Forward (+)
10994283 .. 11002285
8003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG056100.1

Sequence Viewer

Length: 498 bp
ATGGCGGAAGAGGAACATATAACAAATTGTCATATAATTGATGCCATAGTCGCACCAAAAGTTGGCGAAGATAGTGACAAGGATTCGTCTGAAAAATCAGTAAGGAGTGACAGTGATAGAGAAACTGACGGCAAAACTGCAGTTGACATCAAACCTGCTGCTGAAAATCATGGAACTGATATTGTTAGGTTCACACAGAGGAACAGTCTGAGGATCTACCCCAAAGGAACACTAGTTATCTACTCGAATTACCAATCGCATGTTGGGTGGGTGCATGGATCTCAAATAGTTGTGTTTAATATGCAGGGCCCAAATGATGAGGTTTTTGATCCTAAAAGGACCTTGATAGTGGCAAAGACATTGAAGGTGAAAGCATACATGGGGAATAAATGGCACATGGATTTTAGTCAAACATACTTCAATTCGTTCGCCCCACAGACTTCTACACAAAATTTTGTGGAATTTCTGAAGAGGTGTAGAAGAAGTTTTTCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

165

Amino Acids

18.79

Weight (kDa)

6.59

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-Y PF00387 55 - 106 6.2e-08 Phosphatidylinositol-specific phospholipase C, Y domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 163
AccB7I CCANNNNNTGG 1 cut(s) 62
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 221, 286, 323
AcsI RAATTY 2 cut(s) 451, 461
AcuI CTGAAG 1 cut(s) 488
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 2 cut(s) 364, 421
AhlI ACTAGT 1 cut(s) 232
AlwI GGATC 3 cut(s) 221, 286, 323
AoxI GGCC 1 cut(s) 307
ApaI GGGCCC 1 cut(s) 311
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 451, 461
Asp700I GAANNNNTTC 1 cut(s) 487
AspS9I GGNCC 3 cut(s) 307, 308, 339
AsuHPI GGTGA 1 cut(s) 379
AvaII GGWCC 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 145
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 138
BfuAI ACCTGC 1 cut(s) 163
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 3 cut(s) 307, 308, 339
BmiI GGNNCC 1 cut(s) 309
BmsI GCATC 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 62
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 1 cut(s) 145
BshFI GGCC 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 62
BsnI GGCC 1 cut(s) 309
Bsp120I GGGCCC 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 311
Bsp143I GATC 3 cut(s) 213, 278, 328
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 309
BspMAI CTGCAG 1 cut(s) 142
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 3 cut(s) 221, 286, 323
BssMI GATC 3 cut(s) 213, 278, 328
Bst4CI ACNGT 2 cut(s) 113, 206
Bst6I CTCTTC 2 cut(s) 3, 464
BstDEI CTNAG 1 cut(s) 209
BstKTI GATC 3 cut(s) 216, 281, 331
BstMBI GATC 3 cut(s) 213, 278, 328
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 263
BstSFI CTRYAG 1 cut(s) 138
BstSLI GKGCMC 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 145
BstX2I RGATCY 2 cut(s) 213, 278
BstYI RGATCY 2 cut(s) 213, 278
BsuRI GGCC 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 118
BveI ACCTGC 1 cut(s) 163
Cfr13I GGNCC 3 cut(s) 307, 308, 339
CviAII CATG 5 cut(s) 170, 260, 275, 379, 397
CviJI RGCY 1 cut(s) 309
CviKI_1 RGCY 1 cut(s) 309
DdeI CTNAG 1 cut(s) 209
DpnI GATC 3 cut(s) 215, 280, 330
DpnII GATC 3 cut(s) 213, 278, 328
Eam1104I CTCTTC 2 cut(s) 3, 464
EarI CTCTTC 2 cut(s) 3, 464
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 488
EcoO109I RGGNCCY 2 cut(s) 307, 339
EcoT38I GRGCYC 1 cut(s) 311
FaeI CATG 5 cut(s) 173, 263, 278, 382, 400
FatI CATG 5 cut(s) 169, 259, 274, 378, 396
Fnu4HI GCNGC 1 cut(s) 159
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 233
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 1 cut(s) 309
Hin1II CATG 5 cut(s) 173, 263, 278, 382, 400
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 1 cut(s) 83
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 2 cut(s) 145, 192
Hpy188I TCNGA 4 cut(s) 91, 210, 468, 493
Hpy8I GTNNAC 2 cut(s) 145, 192
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 113, 206
HpyCH4V TGCA 3 cut(s) 140, 274, 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 209
Hsp92II CATG 5 cut(s) 173, 263, 278, 382, 400
Kzo9I GATC 3 cut(s) 213, 278, 328
LpnPI CCDG 2 cut(s) 168, 290
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 1 cut(s) 31
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 74, 107
MalI GATC 3 cut(s) 215, 280, 330
MboI GATC 3 cut(s) 213, 278, 328
MboII GAAGA 4 cut(s) 20, 80, 481, 492
MflI RGATCY 2 cut(s) 213, 278
MhlI GDGCHC 1 cut(s) 311
MluCI AATT 6 cut(s) 25, 36, 247, 421, 451, 461
MnlI CCTC 5 cut(s) 4, 192, 204, 313, 465
MroXI GAANNNNTTC 1 cut(s) 487
MseI TTAA 2 cut(s) 297, 496
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 3 cut(s) 213, 278, 328
NlaIII CATG 5 cut(s) 173, 263, 278, 382, 400
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 2 cut(s) 74, 107
NspI RCATGY 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 487
PfeI GAWTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 62
PkrI GCNGC 1 cut(s) 160
PpuMI RGGWCCY 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 309
PspOMI GGGCCC 1 cut(s) 307
PspPI GGNCC 3 cut(s) 307, 308, 339
PspPPI RGGWCCY 1 cut(s) 339
PstI CTGCAG 1 cut(s) 142
PsuI RGATCY 2 cut(s) 213, 278
SaqAI TTAA 2 cut(s) 297, 496
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 3 cut(s) 213, 278, 328
Sau96I GGNCC 3 cut(s) 307, 308, 339
SduI GDGCHC 1 cut(s) 311
SetI ASST 6 cut(s) 157, 191, 324, 344, 369, 476
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 138
SinI GGWCC 1 cut(s) 339
SpeI ACTAGT 1 cut(s) 232
Sse9I AATT 6 cut(s) 25, 36, 247, 421, 451, 461
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 233
TaaI ACNGT 2 cut(s) 113, 206
TaqI TCGA 1 cut(s) 245
TasI AATT 6 cut(s) 25, 36, 247, 421, 451, 461
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 297, 496
Tru9I TTAA 2 cut(s) 297, 496
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 2 cut(s) 74, 107
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 2 cut(s) 74, 107
TspRI CASTG 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 339
XapI RAATTY 2 cut(s) 451, 461
XceI RCATGY 1 cut(s) 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 487
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.