Rh4DG000700

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008400
Physical Location & Seq
Reverse (-)
221201 .. 224162
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG000700.1

Sequence Viewer

Length: 1380 bp
ATGAATAAAGTAATGGACAAGCTCTTACAAAAGGAATTGGAACATGTTTCTGATGATGCTGATCACAATACTACCGCCTCCCCTCTGCCTCTGCCTAGTCAACAACATCGTGAGCTTCATCTTCCTACTGAAGCGGAAGCTGAGGAAGACGACCTCATAATTAATATTGTGTCAAGTAAACATGAAGAAAACATAGTATCGGCCTTGGAGGAATTGAGATCCAGCGGAACACAGGTGTGCAGTAAGATTAATGGACCTCCTAACAAAAAGAGGAAATCCCTTCTCAACAAGAATCACAATGAAATAGAATTGGAGCCTGATATCCCTGGCTCAAATACTGATTCTAAATCTCAACCAGATAAACCAGAGCTGGGTGTCCAACAATCCACTGCCCCGGTTTCATGGTCTCAAAAATGTTCATGGAGACAACTTGTCGGTCATAGGGACAACAGTGGGTTCAGTGTCTCACATATACTGACAGGCAGATCTTCTTCTAGTGACCAAATGGAGCCCAAATCTGGCAGTTCCGATGTGCACCAGTCTGAGGACCTGGCAAGTGACGGAAACTCAGAAGAACTGCTGTCTGAGATGGAACCACTTAAGGGAAGATCAGAACATAAACCTACCAAGTCGGTATCTGACTCATATGAGTCGGGTATAGTTCCTTCATGGCATCAGGAATTATCTCTGACACAACTGACTGATGAGAATACTGGGAGTGTAACTGAACAGGCCCAGCCAAACTCTGGTTCTGGTGCTTTTGAAGTGCCCCATTCTGACAGCAAAAACCAGGACTTGGCAAGAGATGGAAACTTGGAAGGCCAGCTCTGTGAGATGGAACTGGTGGAGGAGAATGCGGATCCTCAACCTCAAAATGCACTATCAAACTCAACTAAGTCAGGTAGAGGAGCTTCATGGCGCCAGAAATCGTCCTGGACACAATTGATTAATGACAATACTGGAAGCTCTTTCAGCATAACACAAGTTCTACCAGCTGGTATCTCTTTCAAGCAACAAGGGTTCCAAAAGCCCAGAGGTGTAGATGTCATGAGCTCTTTTGGCAGCAAGCTCAAGGAAACAGTAAAGCAAGATAAGAATAAACTTACTAGAGGTGAATCCTCTTCTTTGGGGATAATTGGAAAGGAGGGGGATGGTTTACCACGGAGTACTCCTAAAGAGAATCAACAGATTATTGCTGGCAATGACAATGCTCCAGATCTGGGAAAAATATGTGATTTGACACCAAAGCAAGCATCTACAGGCACTGTTGACAAAGGTAAAACCTGCTTATTTGTGAGAAGTGCTGCTTCATTAAATGAATGGGCGAAAGCTAAGGCTGCCCTAAGTGGGTCACGAAAAAGAAAGAACAGCAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

459

Amino Acids

49.87

Weight (kDa)

5.57

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1292
AccB1I GGYRCC 1 cut(s) 918
AccB7I CCANNNNNTGG 2 cut(s) 746, 796
AciI CCGC 4 cut(s) 75, 134, 225, 857
AclWI GGATC 3 cut(s) 213, 854, 867
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 919
AfaI GTAC 1 cut(s) 1168
AfiI CCNNNNNNNGG 8 cut(s) 371, 518, 544, 602, 746, 796, 1220, 1347
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 2 cut(s) 764, 1009
AhdI GACNNNNNGTC 1 cut(s) 431
AjnI CCWGG 4 cut(s) 325, 549, 789, 932
AjuI GAANNNNNNNTTGG 2 cut(s) 733, 765
AleI CACNNNNGTG 1 cut(s) 235
Alw21I GWGCWC 2 cut(s) 537, 1055
Alw26I GTCTC 3 cut(s) 411, 418, 469
Alw44I GTGCAC 1 cut(s) 533
AlwI GGATC 3 cut(s) 213, 854, 867
AlwNI CAGNNNCTG 1 cut(s) 1265
AoxI GGCC 3 cut(s) 201, 732, 820
ApaLI GTGCAC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 1062, 1304, 1337
AseI ATTAAT 3 cut(s) 162, 249, 948
AspLEI GCGC 1 cut(s) 921
AspS9I GGNCC 3 cut(s) 254, 547, 733
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 1124
AvaII GGWCC 2 cut(s) 254, 547
BaeGI GKGCMC 2 cut(s) 537, 771
BamHI GGATCC 1 cut(s) 859
BanI GGYRCC 1 cut(s) 918
BanII GRGCYC 2 cut(s) 513, 1055
BarI GAAGNNNNNNTAC 4 cut(s) 649, 681, 895, 927
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 2 cut(s) 537, 1055
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 1074, 1291, 1324
BccI CCATC 4 cut(s) 583, 800, 829, 1145
BciT130I CCWGG 4 cut(s) 327, 551, 791, 934
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 3 cut(s) 411, 418, 469
BfaI CTAG 3 cut(s) 96, 495, 1107
BfmI CTRYAG 1 cut(s) 1257
BfoI RGCGCY 1 cut(s) 922
BfrI CTTAAG 1 cut(s) 599
BfuAI ACCTGC 1 cut(s) 1292
BglII AGATCT 2 cut(s) 485, 1216
BisI GCNGC 3 cut(s) 1063, 1305, 1338
BlsI GCNGC 3 cut(s) 1064, 1306, 1339
BmcAI AGTACT 1 cut(s) 1168
Bme1390I CCNGG 5 cut(s) 327, 395, 551, 791, 934
Bme18I GGWCC 2 cut(s) 254, 547
BmeRI GACNNNNNGTC 1 cut(s) 431
BmgT120I GGNCC 3 cut(s) 254, 547, 733
BmiI GGNNCC 6 cut(s) 315, 510, 594, 861, 920, 1022
BmrFI CCNGG 5 cut(s) 327, 395, 551, 791, 934
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 3 cut(s) 46, 682, 1262
BmuI ACTGGG 1 cut(s) 723
BpiI GAAGAC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 1197
Bpu10I CCTNAGC 2 cut(s) 141, 1332
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 919
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 204, 325, 393, 1160
Bsc4I CCNNNNNNNGG 8 cut(s) 371, 518, 544, 602, 746, 796, 1220, 1347
Bse1I ACTGG 4 cut(s) 538, 718, 846, 964
Bse3DI GCAATG 1 cut(s) 1207
Bse8I GATNNNNATC 1 cut(s) 60
BseBI CCWGG 4 cut(s) 327, 551, 791, 934
BseDI CCNNGG 4 cut(s) 204, 325, 393, 1160
BseGI GGATG 1 cut(s) 1156
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 8 cut(s) 371, 518, 544, 602, 746, 796, 1220, 1347
BseMI GCAATG 1 cut(s) 1207
BseMII CTCAG 4 cut(s) 132, 534, 576, 582
BseNI ACTGG 4 cut(s) 538, 718, 846, 964
BseRI GAGGAG 2 cut(s) 863, 921
BseSI GKGCMC 2 cut(s) 537, 771
BseXI GCAGC 3 cut(s) 1074, 1291, 1324
BseYI CCCAGC 2 cut(s) 370, 735
BsgI GTGCAG 1 cut(s) 259
BshFI GGCC 3 cut(s) 203, 734, 822
BshNI GGYRCC 1 cut(s) 918
BsiHKAI GWGCWC 2 cut(s) 537, 1055
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 8 cut(s) 371, 518, 544, 602, 746, 796, 1220, 1347
BsmAI GTCTC 3 cut(s) 411, 418, 469
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 3 cut(s) 203, 734, 822
Bso31I GGTCTC 1 cut(s) 411
Bsp1286I GDGCHC 4 cut(s) 513, 537, 771, 1055
Bsp143I GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
BspACI CCGC 4 cut(s) 75, 134, 225, 857
BspANI GGCC 3 cut(s) 203, 734, 822
BspCNI CTCAG 4 cut(s) 133, 535, 577, 581
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 6 cut(s) 315, 510, 594, 861, 920, 1022
BspMI ACCTGC 1 cut(s) 1292
BspPI GGATC 3 cut(s) 213, 854, 867
BspT107I GGYRCC 1 cut(s) 918
BspTI CTTAAG 1 cut(s) 599
BspTNI GGTCTC 1 cut(s) 411
BsrDI GCAATG 1 cut(s) 1207
BsrI ACTGG 4 cut(s) 538, 718, 846, 964
BssECI CCNNGG 4 cut(s) 204, 325, 393, 1160
BssMI GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
BssNI GRCGYC 1 cut(s) 919
BssT1I CCWWGG 1 cut(s) 204
Bst2UI CCWGG 4 cut(s) 327, 551, 791, 934
Bst4CI ACNGT 3 cut(s) 452, 1081, 1267
Bst6I CTCTTC 1 cut(s) 1126
BstACI GRCGYC 1 cut(s) 919
BstAFI CTTAAG 1 cut(s) 599
BstC8I GCNNGC 4 cut(s) 824, 1067, 1198, 1251
BstDEI CTNAG 7 cut(s) 141, 543, 568, 585, 894, 1332, 1343
BstDSI CCRYGG 1 cut(s) 1160
BstF5I GGATG 1 cut(s) 1156
BstH2I RGCGCY 1 cut(s) 922
BstHHI GCGC 1 cut(s) 921
BstKTI GATC 6 cut(s) 64, 221, 488, 611, 862, 1219
BstMAI GTCTC 3 cut(s) 411, 418, 469
BstMBI GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 972, 1059, 1337
BstNI CCWGG 4 cut(s) 327, 551, 791, 934
BstNSI RCATGY 1 cut(s) 47
BstSCI CCNGG 5 cut(s) 325, 393, 549, 789, 932
BstSFI CTRYAG 1 cut(s) 1257
BstSLI GKGCMC 2 cut(s) 537, 771
BstV1I GCAGC 3 cut(s) 1074, 1291, 1324
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 4 cut(s) 218, 485, 859, 1216
BstYI RGATCY 4 cut(s) 218, 485, 859, 1216
BsuRI GGCC 3 cut(s) 203, 734, 822
BtgI CCRYGG 1 cut(s) 1160
BtsCI GGATG 1 cut(s) 1156
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 4 cut(s) 387, 457, 466, 1263
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 4 cut(s) 824, 1067, 1198, 1251
CaiI CAGNNNCTG 1 cut(s) 1265
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 1 cut(s) 921
Cfr13I GGNCC 3 cut(s) 254, 547, 733
Csp6I GTAC 1 cut(s) 1167
CviAII CATG 7 cut(s) 44, 182, 402, 420, 669, 915, 1048
CviQI GTAC 1 cut(s) 1167
DdeI CTNAG 7 cut(s) 141, 543, 568, 585, 894, 1332, 1343
DinI GGCGCC 1 cut(s) 920
DpnI GATC 6 cut(s) 63, 220, 487, 610, 861, 1218
DpnII GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
DriI GACNNNNNGTC 1 cut(s) 431
Eam1104I CTCTTC 1 cut(s) 1126
Eam1105I GACNNNNNGTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 1126
Ecl136II GAGCTC 1 cut(s) 1053
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 2 cut(s) 513, 1055
Eco31I GGTCTC 1 cut(s) 411
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 254, 547
Eco53kI GAGCTC 1 cut(s) 1053
Eco57I CTGAAG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 1053
EcoO109I RGGNCCY 1 cut(s) 547
EcoRII CCWGG 4 cut(s) 325, 549, 789, 932
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 2 cut(s) 513, 1055
EgeI GGCGCC 1 cut(s) 920
EheI GGCGCC 1 cut(s) 920
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 7 cut(s) 47, 185, 405, 423, 672, 918, 1051
FalI AAGNNNNNCTT 2 cut(s) 1291, 1323
FaqI GGGAC 1 cut(s) 458
FatI CATG 7 cut(s) 43, 181, 401, 419, 668, 914, 1047
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 3 cut(s) 1063, 1305, 1338
FokI GGATG 1 cut(s) 1163
FriOI GRGCYC 2 cut(s) 513, 1055
Fsp4HI GCNGC 3 cut(s) 1063, 1305, 1338
FspBI CTAG 3 cut(s) 96, 495, 1107
GlaI GCGC 1 cut(s) 920
GluI GCNGC 3 cut(s) 1063, 1305, 1338
GsaI CCCAGC 2 cut(s) 374, 739
GsuI CTGGAG 1 cut(s) 1197
HaeII RGCGCY 1 cut(s) 922
HaeIII GGCC 3 cut(s) 203, 734, 822
HapII CCGG 1 cut(s) 395
HhaI GCGC 1 cut(s) 921
Hin1I GRCGYC 1 cut(s) 919
Hin1II CATG 7 cut(s) 47, 185, 405, 423, 672, 918, 1051
Hin6I GCGC 1 cut(s) 919
HinP1I GCGC 1 cut(s) 919
HincII GTYRAC 2 cut(s) 101, 1270
HindII GTYRAC 2 cut(s) 101, 1270
HinfI GANTC 6 cut(s) 292, 341, 641, 650, 1115, 1180
HpaII CCGG 1 cut(s) 395
HphI GGTGA 1 cut(s) 1124
Hpy166II GTNNAC 5 cut(s) 101, 179, 535, 1157, 1270
Hpy188I TCNGA 9 cut(s) 52, 529, 544, 571, 586, 613, 640, 690, 778
Hpy188III TCNNGA 5 cut(s) 110, 677, 1048, 1214, 1353
Hpy8I GTNNAC 5 cut(s) 101, 179, 535, 1157, 1270
HpyAV CCTTC 3 cut(s) 290, 675, 812
HpyCH4III ACNGT 3 cut(s) 452, 1081, 1267
HpyCH4V TGCA 3 cut(s) 240, 535, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 972, 1059, 1337
HpyF3I CTNAG 7 cut(s) 141, 543, 568, 585, 894, 1332, 1343
Hsp92I GRCGYC 1 cut(s) 919
Hsp92II CATG 7 cut(s) 47, 185, 405, 423, 672, 918, 1051
HspAI GCGC 1 cut(s) 919
KasI GGCGCC 1 cut(s) 918
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
LmnI GCTCC 4 cut(s) 313, 508, 908, 1216
Lsp1109I GCAGC 3 cut(s) 1074, 1291, 1324
LweI GCATC 3 cut(s) 46, 682, 1262
MaeI CTAG 3 cut(s) 96, 495, 1107
MaeIII GTNAC 4 cut(s) 497, 557, 721, 1350
MalI GATC 6 cut(s) 63, 220, 487, 610, 861, 1218
MboI GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
MboII GAAGA 8 cut(s) 113, 158, 197, 480, 483, 584, 618, 1113
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 4 cut(s) 218, 485, 859, 1216
MhlI GDGCHC 4 cut(s) 513, 537, 771, 1055
MluCI AATT 7 cut(s) 35, 159, 212, 308, 680, 941, 1134
Mly113I GGCGCC 1 cut(s) 919
MlyI GAGTC 2 cut(s) 635, 659
MmeI TCCRAC 1 cut(s) 403
MseI TTAA 5 cut(s) 162, 249, 600, 948, 1313
MslI CAYNNNNRTG 1 cut(s) 235
MspA1I CMGCKG 2 cut(s) 225, 995
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 5 cut(s) 327, 395, 551, 791, 934
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 4 cut(s) 327, 551, 791, 934
MwoI GCNNNNNNNGC 3 cut(s) 972, 1059, 1337
NarI GGCGCC 1 cut(s) 919
NciI CCSGG 1 cut(s) 395
NdeI CATATG 1 cut(s) 646
NdeII GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
NlaIII CATG 7 cut(s) 47, 185, 405, 423, 672, 918, 1051
NlaIV GGNNCC 6 cut(s) 315, 510, 594, 861, 920, 1022
NmuCI GTSAC 3 cut(s) 497, 557, 1350
NspI RCATGY 1 cut(s) 47
OliI CACNNNNGTG 1 cut(s) 235
PagI TCATGA 1 cut(s) 1047
PciI ACATGT 1 cut(s) 43
PctI GAATGC 1 cut(s) 859
PfeI GAWTC 4 cut(s) 292, 341, 1115, 1180
PflMI CCANNNNNTGG 2 cut(s) 746, 796
PfoI TCCNGGA 1 cut(s) 932
PkrI GCNGC 3 cut(s) 1064, 1306, 1339
PleI GAGTC 2 cut(s) 635, 658
PluTI GGCGCC 1 cut(s) 922
PpsI GAGTC 2 cut(s) 635, 658
PpuMI RGGWCCY 1 cut(s) 547
PscI ACATGT 1 cut(s) 43
PshBI ATTAAT 3 cut(s) 162, 249, 948
Psp124BI GAGCTC 1 cut(s) 1055
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 4 cut(s) 325, 549, 789, 932
PspFI CCCAGC 2 cut(s) 370, 735
PspGI CCWGG 4 cut(s) 325, 549, 789, 932
PspN4I GGNNCC 6 cut(s) 315, 510, 594, 861, 920, 1022
PspPI GGNCC 3 cut(s) 254, 547, 733
PspPPI RGGWCCY 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 4 cut(s) 218, 485, 859, 1216
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 1 cut(s) 1168
RsaNI GTAC 1 cut(s) 1167
RseI CAYNNNNRTG 1 cut(s) 235
SacI GAGCTC 1 cut(s) 1055
SaqAI TTAA 5 cut(s) 162, 249, 600, 948, 1313
SatI GCNGC 3 cut(s) 1063, 1305, 1338
Sau3AI GATC 6 cut(s) 61, 218, 485, 608, 859, 1216
Sau96I GGNCC 3 cut(s) 254, 547, 733
ScaI AGTACT 1 cut(s) 1168
SchI GAGTC 2 cut(s) 635, 659
ScrFI CCNGG 5 cut(s) 327, 395, 551, 791, 934
SduI GDGCHC 4 cut(s) 513, 537, 771, 1055
SfaNI GCATC 3 cut(s) 46, 682, 1262
SfcI CTRYAG 1 cut(s) 1257
SfoI GGCGCC 1 cut(s) 920
SinI GGWCC 2 cut(s) 254, 547
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 599, 1070
SmoI CTYRAG 2 cut(s) 599, 1070
Sse9I AATT 7 cut(s) 35, 159, 212, 308, 680, 941, 1134
SsiI CCGC 4 cut(s) 75, 134, 225, 857
SspDI GGCGCC 1 cut(s) 918
SspI AATATT 1 cut(s) 166
SspMI CTAG 3 cut(s) 96, 495, 1107
SstI GAGCTC 1 cut(s) 1055
StyD4I CCNGG 5 cut(s) 325, 393, 549, 789, 932
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 3 cut(s) 452, 1081, 1267
TaqII GACCGA 1 cut(s) 425
TasI AATT 7 cut(s) 35, 159, 212, 308, 680, 941, 1134
TatI WGTACW 1 cut(s) 1166
TfiI GAWTC 4 cut(s) 292, 341, 1115, 1180
Tru1I TTAA 5 cut(s) 162, 249, 600, 948, 1313
Tru9I TTAA 5 cut(s) 162, 249, 600, 948, 1313
TscAI CASTG 4 cut(s) 394, 457, 466, 1270
TseFI GTSAC 3 cut(s) 497, 557, 1350
TseI GCWGC 3 cut(s) 1062, 1304, 1337
Tsp45I GTSAC 3 cut(s) 497, 557, 1350
TspGWI ACGGA 2 cut(s) 576, 1177
TspRI CASTG 4 cut(s) 394, 457, 466, 1270
Van91I CCANNNNNTGG 2 cut(s) 746, 796
Vha464I CTTAAG 1 cut(s) 599
VneI GTGCAC 1 cut(s) 533
VpaK11BI GGWCC 2 cut(s) 254, 547
VspI ATTAAT 3 cut(s) 162, 249, 948
XceI RCATGY 1 cut(s) 47
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XspI CTAG 3 cut(s) 96, 495, 1107
ZrmI AGTACT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.