Rh4DG132000

Peptidyl-prolyl cis-trans isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007395
Physical Location & Seq
Reverse (-)
22245278 .. 22253303
8026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG132000.1

Sequence Viewer

Length: 708 bp
ATGGCCACAGCGTTGCCCATGGCATCGACTTCCAGTCTCACTCTCAGTCTCAGTCTCGGTAGACGACCACCCTTATTGGGTTCATATTCTGGAAAGAGGACCATGCCTGCAAATTATCAAGGCATTTGCTGTCTCAATAATTCACAACTTTGTACAACCACAAGAGGATACAAGGATGACGTTAATCTAATCAAACGGAGACAGATGATTGCCTTCCTTTTTGGTTTTTCAAGCTTTGTCTTAGACTCATTTCAAGCCGAGGGTGCTGGCTTGCCCCCTGAAGAAAAGCCTCGACTATGTGATGATTCTTGCGAAAAACAGCTAGAAAATGTCCCTATGGTAACTACAGAGTCTGGTTTGCAGTATAAGGATATTAAAGTTGGTCAAGGCCCCAGTCCACCTGTCGGTTTTCAGGTGGCGGCGAATTATGTTGCCATGGTTCCGTCTGGACAAATATTTGACAGTTCATTGGAGAAGGGTCAACTGTATATATTCCGTGTTGGCTCTGGTCAGGTGATCAAAGGACTTGACGAAGGGATCTTAACCATGAAAATAGGAGGGAAGCGGCGACTCTACATCCCGGGATCTTTGGCCTTTCCCAAGGGTCTCACTTCAGCTCCAGGAAGGCCAAGGGTGGCTCCAAGTAGTCCAGTGGTTTTCGATGTGAGTTTGGAATACGTACCAGGCCTTGAAATTGAGGAAGAGTGA

Protein Analysis

235

Amino Acids

25.36

Weight (kDa)

8.23

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKBP_C PF00254 133 - 224 5.5e-20 FKBP-type peptidyl-prolyl cis-trans isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 61
AciI CCGC 2 cut(s) 419, 565
AclWI GGATC 2 cut(s) 545, 592
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 300, 597
AfaI GTAC 2 cut(s) 154, 681
AfiI CCNNNNNNNGG 2 cut(s) 77, 404
AgsI TTSAA 3 cut(s) 231, 254, 692
AhdI GACNNNNNGTC 1 cut(s) 33
AjnI CCWGG 2 cut(s) 619, 682
AluBI AGCT 3 cut(s) 234, 322, 617
AluI AGCT 3 cut(s) 234, 322, 617
Alw26I GTCTC 6 cut(s) 41, 53, 59, 137, 193, 611
AlwI GGATC 2 cut(s) 545, 592
AlwNI CAGNNNCTG 1 cut(s) 353
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 5 cut(s) 3, 388, 591, 626, 685
AspS9I GGNCC 2 cut(s) 99, 389
AsuC2I CCSGG 2 cut(s) 581, 582
AsuHPI GGTGA 1 cut(s) 526
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 1 cut(s) 99
BalI TGGCCA 1 cut(s) 5
BciT130I CCWGG 2 cut(s) 621, 684
BciVI GTATCC 1 cut(s) 161
BclI TGATCA 1 cut(s) 516
BcnI CCSGG 2 cut(s) 581, 582
BcoDI GTCTC 6 cut(s) 41, 53, 59, 137, 193, 611
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 345
BfuI GTATCC 1 cut(s) 161
BisI GCNGC 2 cut(s) 420, 566
BlsI GCNGC 2 cut(s) 421, 567
Bme1390I CCNGG 4 cut(s) 581, 582, 621, 684
Bme18I GGWCC 1 cut(s) 99
BmeRI GACNNNNNGTC 1 cut(s) 33
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 99, 389
BmiI GGNNCC 3 cut(s) 391, 441, 639
BmrFI CCNGG 4 cut(s) 581, 582, 621, 684
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 603
BpuMI CCSGG 2 cut(s) 581, 582
BsaAI YACGTR 1 cut(s) 679
BsaI GGTCTC 1 cut(s) 611
BsaJI CCNNGG 6 cut(s) 18, 258, 435, 580, 600, 629
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 404
Bse1I ACTGG 3 cut(s) 33, 393, 650
BseBI CCWGG 2 cut(s) 621, 684
BseDI CCNNGG 6 cut(s) 18, 258, 435, 580, 600, 629
BseGI GGATG 2 cut(s) 181, 576
BseLI CCNNNNNNNGG 2 cut(s) 77, 404
BseMII CTCAG 2 cut(s) 58, 64
BseNI ACTGG 3 cut(s) 33, 393, 650
BshFI GGCC 5 cut(s) 5, 390, 593, 628, 687
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 77, 404
BsmAI GTCTC 6 cut(s) 41, 53, 59, 137, 193, 611
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 5 cut(s) 5, 390, 593, 628, 687
Bso31I GGTCTC 1 cut(s) 611
BsoBI CYCGRG 1 cut(s) 580
Bsp1407I TGTACA 1 cut(s) 152
Bsp143I GATC 3 cut(s) 516, 537, 584
Bsp19I CCATGG 2 cut(s) 18, 435
BspACI CCGC 2 cut(s) 419, 565
BspANI GGCC 5 cut(s) 5, 390, 593, 628, 687
BspCNI CTCAG 2 cut(s) 57, 63
BspLI GGNNCC 3 cut(s) 391, 441, 639
BspPI GGATC 2 cut(s) 545, 592
BspTNI GGTCTC 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 152
BsrI ACTGG 3 cut(s) 33, 393, 650
BssECI CCNNGG 6 cut(s) 18, 258, 435, 580, 600, 629
BssMI GATC 3 cut(s) 516, 537, 584
BssT1I CCWWGG 4 cut(s) 18, 435, 600, 629
Bst2UI CCWGG 2 cut(s) 621, 684
Bst4CI ACNGT 2 cut(s) 464, 486
Bst6I CTCTTC 1 cut(s) 696
BstAUI TGTACA 1 cut(s) 152
BstBAI YACGTR 1 cut(s) 679
BstC8I GCNNGC 3 cut(s) 108, 268, 272
BstDEI CTNAG 3 cut(s) 44, 50, 241
BstDSI CCRYGG 2 cut(s) 18, 435
BstF5I GGATG 2 cut(s) 181, 576
BstKTI GATC 3 cut(s) 519, 540, 587
BstMAI GTCTC 6 cut(s) 41, 53, 59, 137, 193, 611
BstMBI GATC 3 cut(s) 516, 537, 584
BstMWI GCNNNNNNNGC 1 cut(s) 263
BstNI CCWGG 2 cut(s) 621, 684
BstSCI CCNGG 4 cut(s) 579, 580, 619, 682
BstSFI CTRYAG 1 cut(s) 345
BstSNI TACGTA 1 cut(s) 679
BstX2I RGATCY 2 cut(s) 537, 584
BstYI RGATCY 2 cut(s) 537, 584
BsuI GTATCC 1 cut(s) 161
BsuRI GGCC 5 cut(s) 5, 390, 593, 628, 687
BtgI CCRYGG 2 cut(s) 18, 435
BtsCI GGATG 2 cut(s) 181, 576
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 3 cut(s) 108, 268, 272
CaiI CAGNNNCTG 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 99, 389
Cfr9I CCCGGG 1 cut(s) 580
Csp6I GTAC 2 cut(s) 153, 680
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 4 cut(s) 19, 103, 436, 547
CviQI GTAC 2 cut(s) 153, 680
DdeI CTNAG 3 cut(s) 44, 50, 241
DpnI GATC 3 cut(s) 518, 539, 586
DpnII GATC 3 cut(s) 516, 537, 584
DriI GACNNNNNGTC 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 696
Eam1105I GACNNNNNGTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 696
Eco105I TACGTA 1 cut(s) 679
Eco130I CCWWGG 4 cut(s) 18, 435, 600, 629
Eco147I AGGCCT 1 cut(s) 687
Eco31I GGTCTC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 99
Eco57I CTGAAG 2 cut(s) 300, 597
Eco88I CYCGRG 1 cut(s) 580
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 619, 682
EcoT14I CCWWGG 4 cut(s) 18, 435, 600, 629
ErhI CCWWGG 4 cut(s) 18, 435, 600, 629
FaeI CATG 4 cut(s) 22, 106, 439, 550
FaqI GGGAC 1 cut(s) 317
FatI CATG 4 cut(s) 18, 102, 435, 546
FbaI TGATCA 1 cut(s) 516
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 2 cut(s) 420, 566
FokI GGATG 2 cut(s) 188, 563
Fsp4HI GCNGC 2 cut(s) 420, 566
FspBI CTAG 1 cut(s) 323
GluI GCNGC 2 cut(s) 420, 566
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 5 cut(s) 5, 390, 593, 628, 687
HapII CCGG 1 cut(s) 581
Hin1II CATG 4 cut(s) 22, 106, 439, 550
HincII GTYRAC 1 cut(s) 482
HindII GTYRAC 1 cut(s) 482
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 4 cut(s) 245, 305, 350, 570
HpaII CCGG 1 cut(s) 581
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 3 cut(s) 62, 398, 482
Hpy188III TCNNGA 2 cut(s) 90, 447
Hpy8I GTNNAC 3 cut(s) 62, 398, 482
HpyAV CCTTC 4 cut(s) 223, 469, 527, 618
HpyCH4III ACNGT 2 cut(s) 464, 486
HpyCH4IV ACGT 2 cut(s) 180, 678
HpyCH4V TGCA 2 cut(s) 110, 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 263
HpyF3I CTNAG 3 cut(s) 44, 50, 241
HpySE526I ACGT 2 cut(s) 180, 678
Hsp92II CATG 4 cut(s) 22, 106, 439, 550
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 3 cut(s) 516, 537, 584
LmnI GCTCC 2 cut(s) 622, 643
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 180, 678
MaeIII GTNAC 1 cut(s) 340
MalI GATC 3 cut(s) 518, 539, 586
MboI GATC 3 cut(s) 516, 537, 584
MboII GAAGA 1 cut(s) 293
MflI RGATCY 2 cut(s) 537, 584
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 112, 139, 424, 693
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 239, 359, 564
MnlI CCTC 6 cut(s) 90, 158, 253, 300, 551, 691
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 183, 375, 542
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 4 cut(s) 581, 582, 621, 684
MvaI CCWGG 2 cut(s) 621, 684
MwoI GCNNNNNNNGC 1 cut(s) 263
NciI CCSGG 2 cut(s) 581, 582
NcoI CCATGG 2 cut(s) 18, 435
NdeII GATC 3 cut(s) 516, 537, 584
NlaIII CATG 4 cut(s) 22, 106, 439, 550
NlaIV GGNNCC 3 cut(s) 391, 441, 639
NmeAIII GCCGAG 1 cut(s) 283
PceI AGGCCT 1 cut(s) 687
PfeI GAWTC 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 619
PkrI GCNGC 2 cut(s) 421, 567
PleI GAGTC 3 cut(s) 239, 358, 564
PpsI GAGTC 3 cut(s) 239, 358, 564
Ppu21I YACGTR 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 619, 682
PspGI CCWGG 2 cut(s) 619, 682
PspN4I GGNNCC 3 cut(s) 391, 441, 639
PspPI GGNCC 2 cut(s) 99, 389
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 2 cut(s) 537, 584
RsaI GTAC 2 cut(s) 154, 681
RsaNI GTAC 2 cut(s) 153, 680
SaqAI TTAA 3 cut(s) 183, 375, 542
SatI GCNGC 2 cut(s) 420, 566
Sau3AI GATC 3 cut(s) 516, 537, 584
Sau96I GGNCC 2 cut(s) 99, 389
SchI GAGTC 3 cut(s) 239, 359, 564
ScrFI CCNGG 4 cut(s) 581, 582, 621, 684
SetI ASST 8 cut(s) 183, 236, 324, 403, 417, 516, 619, 681
SfaNI GCATC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 345
SinI GGWCC 1 cut(s) 99
SmaI CCCGGG 1 cut(s) 582
SnaBI TACGTA 1 cut(s) 679
Sse9I AATT 4 cut(s) 112, 139, 424, 693
SseBI AGGCCT 1 cut(s) 687
SsiI CCGC 2 cut(s) 419, 565
SspI AATATT 1 cut(s) 456
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 687
StyD4I CCNGG 4 cut(s) 579, 580, 619, 682
StyI CCWWGG 4 cut(s) 18, 435, 600, 629
TaaI ACNGT 2 cut(s) 464, 486
TaiI ACGT 2 cut(s) 183, 681
TaqI TCGA 3 cut(s) 26, 292, 660
TasI AATT 4 cut(s) 112, 139, 424, 693
TatI WGTACW 1 cut(s) 152
TauI GCSGC 2 cut(s) 422, 568
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 3 cut(s) 183, 375, 542
Tru9I TTAA 3 cut(s) 183, 375, 542
TscAI CASTG 1 cut(s) 657
TspDTI ATGAA 3 cut(s) 72, 456, 563
TspGWI ACGGA 3 cut(s) 211, 432, 485
TspMI CCCGGG 1 cut(s) 580
TspRI CASTG 1 cut(s) 657
VpaK11BI GGWCC 1 cut(s) 99
XmaI CCCGGG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 61
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.