Rh4DG438700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014731
Physical Location & Seq
Forward (+)
64132775 .. 64137175
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG438700.1

Sequence Viewer

Length: 558 bp
ATGGGATATGACTCTGCTTTGCCAGACAGAGTTAAGGTTTGCTTCGAACTACTAGTGGGTAAATACAGGGAAGAAGAGAAGGCGTATGGTTATGAATCATCCATGCATGATTTTAATAATGACACTAGTGCTTCAGATCATAGAACTCCAAGTGTTGTGTTGGATATTGAGAGCCTTGCACAATCTTCAGATTTCTGCTCAGGGAGCCCCAAAATGACTAGGGCACTCTCGCGGAAATGGTCTTATCGAGCAGAGAGATCGATTAACACTGATCAGGAGGAAGACACAGATCATGAACCAACAAAGAAACTTCTAGTGAAAGTGAACTCTCAGTTGGAGACCTTGAAGCCACCATTGATCACCTCAAAATCATTGCCGTCAACCTCACCTACTCTAATGGGTACTAATCTGCTGGATACAGGGGATGGACGGAGCAAGAGGTTTAATCGCTTTATGACCATCAACCCTCGGAAGATCCTTCTCATATTTGCAACCATGTCCAGTATGGGCACATTAATCTTGATATACTTCACACTCGCCATTAACCGGACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.87

Weight (kDa)

7.75

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014731)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36470
malus_domestica MD13G1009000.v1.1 MD16G1007000.v1.1
prunus_persica Prupe.1G343700_v2.0.a1
pyrus_communis pycom13g00760 pycom16g00660
rosa_chinensis RchiOBHm_Chr4g0446231
rosa_laevigata RLG00000005679
rosa_multiflora Rmu_sc0002266.1_g000011 Rmu_sc0014728.1_g000007
rosa_roxburghii Rroxscaffold_5G00386630
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430800 Rh4BG440200 Rh4CG457500 Rh4DG438700
rosa_wichuraiana Rw4G036800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 232
AciI CCGC 1 cut(s) 232
AclWI GGATC 1 cut(s) 469
AcuI CTGAAG 2 cut(s) 117, 171
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 1 cut(s) 546
AgsI TTSAA 1 cut(s) 346
AhlI ACTAGT 2 cut(s) 52, 125
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 469
AseI ATTAAT 1 cut(s) 515
AsuHPI GGTGA 2 cut(s) 352, 378
AsuII TTCGAA 1 cut(s) 45
BaeGI GKGCMC 2 cut(s) 226, 512
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 288
BccI CCATC 2 cut(s) 419, 467
BceAI ACGGC 1 cut(s) 361
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BciVI GTATCC 1 cut(s) 409
BclI TGATCA 2 cut(s) 271, 357
BcoDI GTCTC 1 cut(s) 332
BcuI ACTAGT 2 cut(s) 52, 125
BfaI CTAG 4 cut(s) 53, 126, 219, 314
BfuI GTATCC 1 cut(s) 409
BmiI GGNNCC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 199
Bpu14I TTCGAA 1 cut(s) 45
Bsa29I ATCGAT 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 1 cut(s) 467
BsaWI WCCGGW 1 cut(s) 546
Bsc4I CCNNNNNNNGG 1 cut(s) 546
Bse1I ACTGG 1 cut(s) 501
Bse3DI GCAATG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 2 cut(s) 98, 430
BseLI CCNNNNNNNGG 1 cut(s) 546
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 2 cut(s) 213, 344
BseNI ACTGG 1 cut(s) 501
BseSI GKGCMC 2 cut(s) 226, 512
Bsh1236I CGCG 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 260
BsiSI CCGG 1 cut(s) 547
BslI CCNNNNNNNGG 1 cut(s) 546
BsmAI GTCTC 1 cut(s) 332
Bso31I GGTCTC 1 cut(s) 332
Bsp119I TTCGAA 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 209, 226, 512
Bsp143I GATC 6 cut(s) 136, 257, 271, 289, 357, 474
BspACI CCGC 1 cut(s) 232
BspCNI CTCAG 2 cut(s) 212, 343
BspDI ATCGAT 1 cut(s) 260
BspFNI CGCG 1 cut(s) 232
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 1 cut(s) 469
BspT104I TTCGAA 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 371
BsrI ACTGG 1 cut(s) 501
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 6 cut(s) 136, 257, 271, 289, 357, 474
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 199, 330
BstF5I GGATG 2 cut(s) 98, 430
BstFNI CGCG 1 cut(s) 232
BstKTI GATC 6 cut(s) 139, 260, 274, 292, 360, 477
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 6 cut(s) 136, 257, 271, 289, 357, 474
BstMWI GCNNNNNNNGC 1 cut(s) 204
BstSLI GKGCMC 2 cut(s) 226, 512
BstUI CGCG 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 474
BstYI RGATCY 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 260
BsuI GTATCC 1 cut(s) 409
BsuTUI ATCGAT 1 cut(s) 260
BtsCI GGATG 2 cut(s) 98, 430
BtsIMutI CAGTG 1 cut(s) 267
CciI TCATGA 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 260
Csp6I GTAC 1 cut(s) 402
CviAII CATG 4 cut(s) 103, 107, 293, 496
CviJI RGCY 3 cut(s) 174, 207, 349
CviKI_1 RGCY 3 cut(s) 174, 207, 349
CviQI GTAC 1 cut(s) 402
DdeI CTNAG 2 cut(s) 199, 330
DpnI GATC 6 cut(s) 138, 259, 273, 291, 359, 476
DpnII GATC 6 cut(s) 136, 257, 271, 289, 357, 474
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 332
Eco57I CTGAAG 2 cut(s) 117, 171
EcoT22I ATGCAT 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 209
FaeI CATG 4 cut(s) 106, 110, 296, 499
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 4 cut(s) 102, 106, 292, 495
FbaI TGATCA 2 cut(s) 271, 357
FokI GGATG 2 cut(s) 85, 437
FriOI GRGCYC 1 cut(s) 209
FspBI CTAG 4 cut(s) 53, 126, 219, 314
HapII CCGG 1 cut(s) 547
Hin1II CATG 4 cut(s) 106, 110, 296, 499
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 2 cut(s) 11, 95
HpaII CCGG 1 cut(s) 547
HphI GGTGA 2 cut(s) 352, 378
Hpy166II GTNNAC 2 cut(s) 325, 381
Hpy188I TCNGA 3 cut(s) 136, 190, 471
Hpy188III TCNNGA 3 cut(s) 275, 293, 520
Hpy8I GTNNAC 2 cut(s) 325, 381
HpyAV CCTTC 2 cut(s) 73, 488
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4V TGCA 3 cut(s) 106, 179, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 204
HpyF3I CTNAG 2 cut(s) 199, 330
Hsp92II CATG 4 cut(s) 106, 110, 296, 499
Ksp22I TGATCA 2 cut(s) 271, 357
Kzo9I GATC 6 cut(s) 136, 257, 271, 289, 357, 474
LmnI GCTCC 2 cut(s) 204, 432
LpnPI CCDG 7 cut(s) 36, 52, 186, 260, 398, 405, 514
MaeI CTAG 4 cut(s) 53, 126, 219, 314
MalI GATC 6 cut(s) 138, 259, 273, 291, 359, 476
MboI GATC 6 cut(s) 136, 257, 271, 289, 357, 474
MboII GAAGA 5 cut(s) 83, 86, 177, 293, 484
MflI RGATCY 1 cut(s) 474
MhlI GDGCHC 3 cut(s) 209, 226, 512
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 141, 315
MnlI CCTC 5 cut(s) 271, 373, 394, 432, 477
Mph1103I ATGCAT 1 cut(s) 108
MseI TTAA 6 cut(s) 33, 114, 264, 444, 515, 543
MspI CCGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 1 cut(s) 204
NdeII GATC 6 cut(s) 136, 257, 271, 289, 357, 474
NlaIII CATG 4 cut(s) 106, 110, 296, 499
NlaIV GGNNCC 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 108
NspV TTCGAA 1 cut(s) 45
PagI TCATGA 1 cut(s) 292
PfeI GAWTC 1 cut(s) 95
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PshBI ATTAAT 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 474
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 33, 114, 264, 444, 515, 543
Sau3AI GATC 6 cut(s) 136, 257, 271, 289, 357, 474
SchI GAGTC 1 cut(s) 5
SduI GDGCHC 3 cut(s) 209, 226, 512
SetI ASST 6 cut(s) 39, 344, 365, 386, 391, 443
SfuI TTCGAA 1 cut(s) 45
SpeI ACTAGT 2 cut(s) 52, 125
SsiI CCGC 1 cut(s) 232
SspMI CTAG 4 cut(s) 53, 126, 219, 314
TaaI ACNGT 1 cut(s) 553
TaqI TCGA 3 cut(s) 45, 247, 260
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 6 cut(s) 33, 114, 264, 444, 515, 543
Tru9I TTAA 6 cut(s) 33, 114, 264, 444, 515, 543
TscAI CASTG 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 108, 309
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 1 cut(s) 274
VspI ATTAAT 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XspI CTAG 4 cut(s) 53, 126, 219, 314
Zsp2I ATGCAT 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.