Rh5AG054800

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012681
Physical Location & Seq
Reverse (-)
4739007 .. 4749876
10870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG054800.1

Sequence Viewer

Length: 585 bp
ATGTACAACAAGGCTGAGGCTAGTTTTTGGACTGCTGAGGAGGTTGATCTCTCCCAGGATGTACAGCAGTGGGAGGCTCTGATTGACTCCAAGAAACACTTTATAAGTCATGTCCTGGCATTTCTGAGGCAAGGTGAGGCCAAGGTTGCTCGACCTAGTTCTGTACCTCCCGTTGCTCTGAAGGTTGGTGGTGGAATGATGAAACTTGGCCCAGTCAAAACAAAGATGCCTTCAGAACTGAGTGATGGGGTTGACATAATAAGCTTATCAATCACTCCCAATAGATGTCCTCCCGGAGTTGCAACATTTTTCAACCCCATAGATATGGCTTCACAATCAGCCGTAAAGGTTGGTATATTTGTTGTCCAAGCAGCAGGAAATACTGGACCATCACCAAATAGCATGTCTTCCTTCAGTCCATGGATCTTCACTGTTGATTCTGCTTCTCATGACAGAACCTACAACAACTCTATAACCCTTGGCACAACGTCACCATTCCTGGAATTGGACTTGCACGCATGGATATCCGTAAGCTTCGCAAATGAACCCGAGTACTTCTGGCTTGATATAATTGTAACTCCCTAA

Protein Analysis

194

Amino Acids

21.12

Weight (kDa)

5.1

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuc_red_sm PF00268 1 - 49 5.5e-14 Ribonucleotide reductase, small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AclWI GGATC 1 cut(s) 431
AcuI CTGAAG 3 cut(s) 200, 216, 397
AfaI GTAC 4 cut(s) 5, 63, 165, 554
AfiI CCNNNNNNNGG 1 cut(s) 505
AgsI TTSAA 1 cut(s) 313
AjnI CCWGG 3 cut(s) 54, 114, 498
AluBI AGCT 2 cut(s) 264, 534
AluI AGCT 2 cut(s) 264, 534
AlwI GGATC 1 cut(s) 431
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 2 cut(s) 138, 208
ApeKI GCWGC 1 cut(s) 371
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspS9I GGNCC 2 cut(s) 209, 386
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 3 cut(s) 146, 384, 483
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 399
BbvCI CCTCAGC 2 cut(s) 15, 36
BbvI GCAGC 1 cut(s) 383
BccI CCATC 2 cut(s) 239, 397
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 3 cut(s) 56, 116, 500
BcnI CCSGG 1 cut(s) 294
BfaI CTAG 2 cut(s) 21, 156
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
BmcAI AGTACT 1 cut(s) 554
Bme1390I CCNGG 4 cut(s) 56, 116, 294, 500
Bme18I GGWCC 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 209, 386
BmrFI CCNGG 4 cut(s) 56, 116, 294, 500
BmrI ACTGGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 399
Bpu10I CCTNAGC 2 cut(s) 15, 36
BpuMI CCSGG 1 cut(s) 294
BsaJI CCNNGG 4 cut(s) 54, 141, 419, 478
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse1I ACTGG 2 cut(s) 212, 388
BseBI CCWGG 3 cut(s) 56, 116, 500
BseDI CCNNGG 4 cut(s) 54, 141, 419, 478
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMII CTCAG 4 cut(s) 6, 27, 116, 230
BseNI ACTGG 2 cut(s) 212, 388
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 383
BshFI GGCC 2 cut(s) 140, 210
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 505
BsnI GGCC 2 cut(s) 140, 210
BsoBI CYCGRG 1 cut(s) 548
Bsp1407I TGTACA 2 cut(s) 3, 61
Bsp143I GATC 2 cut(s) 46, 423
Bsp19I CCATGG 1 cut(s) 419
BspANI GGCC 2 cut(s) 140, 210
BspCNI CTCAG 4 cut(s) 7, 28, 117, 231
BspHI TCATGA 1 cut(s) 448
BspPI GGATC 1 cut(s) 431
BsrGI TGTACA 2 cut(s) 3, 61
BsrI ACTGG 2 cut(s) 212, 388
BssECI CCNNGG 4 cut(s) 54, 141, 419, 478
BssMI GATC 2 cut(s) 46, 423
BssT1I CCWWGG 3 cut(s) 141, 419, 478
Bst2UI CCWGG 3 cut(s) 56, 116, 500
Bst4CI ACNGT 1 cut(s) 433
BstAUI TGTACA 2 cut(s) 3, 61
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 4 cut(s) 15, 36, 125, 239
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 64
BstKTI GATC 2 cut(s) 49, 426
BstMBI GATC 2 cut(s) 46, 423
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 3 cut(s) 56, 116, 500
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 4 cut(s) 54, 114, 292, 498
BstV1I GCAGC 1 cut(s) 383
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 325
BstYI RGATCY 1 cut(s) 423
BsuRI GGCC 2 cut(s) 140, 210
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 2 cut(s) 74, 429
Cac8I GCNNGC 1 cut(s) 516
CciI TCATGA 1 cut(s) 448
Cfr13I GGNCC 2 cut(s) 209, 386
Csp6I GTAC 4 cut(s) 4, 62, 164, 553
CviAII CATG 5 cut(s) 110, 403, 420, 449, 519
CviQI GTAC 4 cut(s) 4, 62, 164, 553
DdeI CTNAG 4 cut(s) 15, 36, 125, 239
DpnI GATC 2 cut(s) 48, 425
DpnII GATC 2 cut(s) 46, 423
Eco130I CCWWGG 3 cut(s) 141, 419, 478
Eco32I GATATC 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 386
Eco57I CTGAAG 3 cut(s) 200, 216, 397
Eco88I CYCGRG 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 54, 114, 498
EcoRV GATATC 1 cut(s) 525
EcoT14I CCWWGG 3 cut(s) 141, 419, 478
ErhI CCWWGG 3 cut(s) 141, 419, 478
FaeI CATG 5 cut(s) 113, 406, 423, 452, 522
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 5 cut(s) 109, 402, 419, 448, 518
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 1 cut(s) 372
FspBI CTAG 2 cut(s) 21, 156
GluI GCNGC 1 cut(s) 372
HaeIII GGCC 2 cut(s) 140, 210
HapII CCGG 1 cut(s) 294
Hin1II CATG 5 cut(s) 113, 406, 423, 452, 522
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 2 cut(s) 262, 532
HinfI GANTC 2 cut(s) 86, 437
HpaII CCGG 1 cut(s) 294
HphI GGTGA 3 cut(s) 146, 384, 483
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 4 cut(s) 81, 126, 180, 235
Hpy188III TCNNGA 1 cut(s) 449
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 3 cut(s) 175, 240, 421
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 2 cut(s) 302, 514
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 4 cut(s) 15, 36, 125, 239
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 5 cut(s) 113, 406, 423, 452, 522
Kzo9I GATC 2 cut(s) 46, 423
Lsp1109I GCAGC 1 cut(s) 383
LweI GCATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 21, 156
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 489, 574
MalI GATC 2 cut(s) 48, 425
MboI GATC 2 cut(s) 46, 423
MboII GAAGA 2 cut(s) 399, 418
MflI RGATCY 1 cut(s) 423
MluCI AATT 2 cut(s) 503, 570
MlyI GAGTC 1 cut(s) 80
MnlI CCTC 8 cut(s) 10, 31, 34, 67, 120, 130, 177, 300
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 4 cut(s) 56, 116, 294, 500
MvaI CCWGG 3 cut(s) 56, 116, 500
MwoI GCNNNNNNNGC 1 cut(s) 146
NciI CCSGG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 419
NdeII GATC 2 cut(s) 46, 423
NlaIII CATG 5 cut(s) 113, 406, 423, 452, 522
NmuCI GTSAC 1 cut(s) 489
NspI RCATGY 1 cut(s) 406
PagI TCATGA 1 cut(s) 448
PfeI GAWTC 1 cut(s) 437
PfoI TCCNGGA 2 cut(s) 292, 498
PkrI GCNGC 1 cut(s) 373
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PsiI TTATAA 1 cut(s) 104
Psp6I CCWGG 3 cut(s) 54, 114, 498
PspGI CCWGG 3 cut(s) 54, 114, 498
PspPI GGNCC 2 cut(s) 209, 386
PsuI RGATCY 1 cut(s) 423
RsaI GTAC 4 cut(s) 5, 63, 165, 554
RsaNI GTAC 4 cut(s) 4, 62, 164, 553
RseI CAYNNNNRTG 1 cut(s) 323
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 2 cut(s) 46, 423
Sau96I GGNCC 2 cut(s) 209, 386
ScaI AGTACT 1 cut(s) 554
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 4 cut(s) 56, 116, 294, 500
SfaNI GCATC 1 cut(s) 216
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 2 cut(s) 503, 570
SspMI CTAG 2 cut(s) 21, 156
StyD4I CCNGG 4 cut(s) 54, 114, 292, 498
StyI CCWWGG 3 cut(s) 141, 419, 478
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 491
TaqI TCGA 1 cut(s) 151
TasI AATT 2 cut(s) 503, 570
TatI WGTACW 3 cut(s) 3, 61, 552
TfiI GAWTC 1 cut(s) 437
TscAI CASTG 2 cut(s) 74, 436
TseFI GTSAC 1 cut(s) 489
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 489
TspDTI ATGAA 2 cut(s) 215, 558
TspGWI ACGGA 1 cut(s) 517
TspRI CASTG 2 cut(s) 74, 436
VpaK11BI GGWCC 1 cut(s) 386
XceI RCATGY 1 cut(s) 406
XspI CTAG 2 cut(s) 21, 156
ZrmI AGTACT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.