Rh5AG292900

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015576
Physical Location & Seq
Forward (+)
40529135 .. 40531562
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG292900.1

Sequence Viewer

Length: 1182 bp
ATGGAATACAGTGACACAGATACGCCGGAGCAATGCCGGGAGCGCCGCCATCGAAGAATCCAGATGAGGCGCTCGAGGTGGTGCTGTTGCGGCCAGCACTTTCACCGCCAGCTGAGTTCATACCGGACTAATTACACTATGACCACTCATGAAATGCCGCAACACCGGAATCCAATGCCTTATTTCGGCAAGTTTTCACTTATAAGGCGTCGTTCGGAGAGCATTATTGACGATACCTTATTTGTGAAGGAGGACTTCTGCCGGCTCGAACTTCTAGGTGGACAGCCTATGCATTTCTTTGCTGTCTTTGATGCTCAAGGAGACCCTCATGTGTCCACACTGTGCAAACAATTGATGCACCAATGCGTGGCGGCGGAGCTGCGGCGCGTGTGCAGCACTCCAGGGCTTGGTGAGAACTTGAGCTTGGGTTTGGAAACGGGAAGCGAGCAGCAGGAAGGGGAGGAGGCCAAGTGGCATGATCTGGTGCGAACAGCCCTAGAGAGGAGCTTTCAGAGGATGAGCCGTCTCAGGCAACACGCTTGCACGTGTGGGAACATTACTGATGATTGCAGGTGTGGATTCATGATCCTCACGCCGCCAGTTGCGGCTGTTGTGGCAATCTTAACAACTCGGAATATTGTCATTGCTAACTCCGGTACCTCACGCGCCGTCCTTGGTCGTGCCGGGAGTTCTTTACCACTCTTTGATAATTCCAAGCCTGAAAGACTAGATGCACAAGAAAGGCTTCGTGCAGTAGATGGTCGGGTTTTCCATCACAACGGCATGCGTGTATATGGAATTCTTAACATGTCTCATTCCGCAGTGCAAACAGGGGATCACTACCTTCTGAATATTTCCATCACAGAAAGAGAGGGAAATGAAGATGAATGCTTGATTCTAGCCAATCATGGTATTTGGGATGCTATATCAGATGACATGGCTTGCAGGGTAGCTAGAACATGTCTCACATTTGAAGATGGGGAAGCTGCTGGAGCTCCAGCCAAAGATGGTCGTCTTTATGGTCCAGACACATGTGATTATGAGACGAACATGTTTTTCTCTTCCAAAACCAACAGTGCAGCTGCAATACTCTGCCGCCTCGCTTTGGGCCGTGGCAGTCGAGGCGATATAAGTGTCATTGTTGTCGATTTAATGGTTAGGTCGAAGTCAAGGCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

393

Amino Acids

44.31

Weight (kDa)

7.55

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 324 8.8e-19 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015576)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24380
malus_domestica MD01G1191100.v1.1
prunus_persica Prupe.2G285600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0043861 RchiOBHm_Chr5g0043871
rosa_laevigata RLG00000034220
rosa_multiflora Rmu_co8273823.1_g000001 Rmu_sc0009227.1_g000016 Rmu_sc0010851.1_g000002
rosa_roxburghii Rroxscaffold_1G00037190
rosa_rugosa Rorug05G0208900
rosa_samantha Rh5AG292900 Rh5BG301000 Rh5CG328600 Rh5DG309900
rosa_wichuraiana Rw5G027160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AarI CACCTGC 1 cut(s) 561
Acc36I ACCTGC 1 cut(s) 561
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccB7I CCANNNNNTGG 2 cut(s) 367, 407
AccII CGCG 2 cut(s) 387, 666
AclWI GGATC 2 cut(s) 580, 843
AcoI YGGCCR 1 cut(s) 91
AcsI RAATTY 1 cut(s) 798
AcvI CACGTG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 208
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 1 cut(s) 658
AfiI CCNNNNNNNGG 5 cut(s) 185, 367, 407, 501, 1105
AflIII ACRYGT 5 cut(s) 545, 807, 959, 1031, 1050
AgsI TTSAA 1 cut(s) 974
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 8 cut(s) 112, 379, 423, 507, 953, 986, 995, 1082
AluI AGCT 8 cut(s) 112, 379, 423, 507, 953, 986, 995, 1082
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 5 cut(s) 315, 530, 816, 968, 1037
AlwI GGATC 2 cut(s) 580, 843
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 3 cut(s) 91, 465, 1108
ApeKI GCWGC 6 cut(s) 379, 393, 448, 986, 1079, 1082
ApoI RAATTY 1 cut(s) 798
Asp700I GAANNNNTTC 1 cut(s) 744
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 4 cut(s) 45, 72, 387, 668
AspS9I GGNCC 2 cut(s) 1022, 1108
AsuC2I CCSGG 2 cut(s) 38, 685
AsuHPI GGTGA 2 cut(s) 95, 422
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 1 cut(s) 1022
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 997
BbrPI CACGTG 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 997
BbvI GCAGC 6 cut(s) 366, 405, 460, 973, 1069, 1091
BccI CCATC 6 cut(s) 57, 752, 780, 866, 971, 1001
BceAI ACGGC 4 cut(s) 507, 653, 796, 1095
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 2 cut(s) 38, 685
BcoDI GTCTC 5 cut(s) 315, 530, 816, 968, 1037
BfaI CTAG 5 cut(s) 275, 497, 728, 899, 954
BfoI RGCGCY 2 cut(s) 46, 73
BfuAI ACCTGC 1 cut(s) 561
Bme1390I CCNGG 3 cut(s) 38, 402, 685
Bme18I GGWCC 1 cut(s) 1022
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 1022, 1108
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 3 cut(s) 38, 402, 685
BmsI GCATC 4 cut(s) 301, 345, 721, 910
BpmI CTGGAG 3 cut(s) 384, 981, 1011
BpuEI CTTGAG 2 cut(s) 300, 439
BpuMI CCSGG 2 cut(s) 38, 685
BsaAI YACGTR 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 401, 673, 1111
BsaWI WCCGGW 3 cut(s) 123, 165, 653
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 367, 407, 501, 1105
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 599
Bse3DI GCAATG 2 cut(s) 38, 642
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 3 cut(s) 401, 673, 1111
BseGI GGATG 2 cut(s) 522, 925
BseLI CCNNNNNNNGG 5 cut(s) 185, 367, 407, 501, 1105
BseMI GCAATG 2 cut(s) 38, 642
BseMII CTCAG 2 cut(s) 104, 541
BseNI ACTGG 1 cut(s) 599
BseRI GAGGAG 2 cut(s) 476, 517
BseXI GCAGC 6 cut(s) 366, 405, 460, 973, 1069, 1091
BsgI GTGCAG 3 cut(s) 412, 771, 1098
Bsh1236I CGCG 2 cut(s) 387, 666
BshFI GGCC 3 cut(s) 93, 467, 1110
BshNI GGYRCC 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 997
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
BslI CCNNNNNNNGG 5 cut(s) 185, 367, 407, 501, 1105
BsmAI GTCTC 5 cut(s) 315, 530, 816, 968, 1037
BsmBI CGTCTC 2 cut(s) 530, 1037
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 3 cut(s) 93, 467, 1110
Bso31I GGTCTC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 997
Bsp143I GATC 3 cut(s) 478, 585, 835
BspANI GGCC 3 cut(s) 93, 467, 1110
BspCNI CTCAG 2 cut(s) 105, 540
BspFNI CGCG 2 cut(s) 387, 666
BspHI TCATGA 2 cut(s) 148, 582
BspLI GGNNCC 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 2 cut(s) 580, 843
BspT107I GGYRCC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 315
BsrDI GCAATG 2 cut(s) 38, 642
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 1 cut(s) 599
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 3 cut(s) 401, 673, 1111
BssMI GATC 3 cut(s) 478, 585, 835
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 11, 342, 1076
Bst6I CTCTTC 1 cut(s) 1066
BstACI GRCGYC 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 7 cut(s) 95, 110, 263, 446, 541, 785, 943
BstDEI CTNAG 2 cut(s) 113, 527
BstDSI CCRYGG 1 cut(s) 1111
BstF5I GGATG 2 cut(s) 522, 925
BstFNI CGCG 2 cut(s) 387, 666
BstH2I RGCGCY 2 cut(s) 46, 73
BstHHI GCGC 4 cut(s) 45, 72, 387, 668
BstKTI GATC 3 cut(s) 481, 588, 838
BstMAI GTCTC 5 cut(s) 315, 530, 816, 968, 1037
BstMBI GATC 3 cut(s) 478, 585, 835
BstMWI GCNNNNNNNGC 6 cut(s) 42, 90, 393, 614, 992, 1122
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 5 cut(s) 787, 811, 963, 1035, 1054
BstSCI CCNGG 3 cut(s) 36, 400, 683
BstUI CGCG 2 cut(s) 387, 666
BstV1I GCAGC 6 cut(s) 366, 405, 460, 973, 1069, 1091
BsuRI GGCC 3 cut(s) 93, 467, 1110
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 2 cut(s) 522, 925
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 4 cut(s) 16, 338, 828, 1081
BveI ACCTGC 1 cut(s) 561
Cac8I GCNNGC 7 cut(s) 95, 110, 263, 446, 541, 785, 943
CciI TCATGA 2 cut(s) 148, 582
CfoI GCGC 4 cut(s) 45, 72, 387, 668
Cfr10I RCCGGY 1 cut(s) 261
Cfr13I GGNCC 2 cut(s) 1022, 1108
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 657
CviQI GTAC 1 cut(s) 657
DdeI CTNAG 2 cut(s) 113, 527
DpnI GATC 3 cut(s) 480, 587, 837
DpnII GATC 3 cut(s) 478, 585, 835
DraIII CACNNNGTG 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 1066
EarI CTCTTC 1 cut(s) 1066
EciI GGCGGA 1 cut(s) 389
Ecl136II GAGCTC 1 cut(s) 995
Eco130I CCWWGG 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 997
Eco31I GGTCTC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 1022
Eco53kI GAGCTC 1 cut(s) 995
Eco72I CACGTG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 995
EcoRI GAATTC 1 cut(s) 798
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 673
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 997
ErhI CCWWGG 1 cut(s) 673
Esp3I CGTCTC 2 cut(s) 530, 1037
FalI AAGNNNNNCTT 4 cut(s) 239, 271, 729, 761
FokI GGATG 2 cut(s) 529, 932
FriOI GRGCYC 1 cut(s) 997
FspBI CTAG 5 cut(s) 275, 497, 728, 899, 954
GlaI GCGC 4 cut(s) 44, 71, 386, 667
GsuI CTGGAG 3 cut(s) 384, 981, 1011
HaeII RGCGCY 2 cut(s) 46, 73
HaeIII GGCC 3 cut(s) 93, 467, 1110
HapII CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
HgaI GACGC 1 cut(s) 197
HhaI GCGC 4 cut(s) 45, 72, 387, 668
Hin1I GRCGYC 1 cut(s) 208
Hin6I GCGC 4 cut(s) 43, 70, 385, 666
HinP1I GCGC 4 cut(s) 43, 70, 385, 666
HinfI GANTC 4 cut(s) 57, 169, 579, 895
HpaII CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
HphI GGTGA 2 cut(s) 95, 422
Hpy166II GTNNAC 2 cut(s) 281, 336
Hpy188I TCNGA 5 cut(s) 217, 513, 633, 849, 931
Hpy188III TCNNGA 4 cut(s) 61, 149, 583, 1025
Hpy8I GTNNAC 2 cut(s) 281, 336
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 3 cut(s) 241, 449, 854
HpyCH4III ACNGT 3 cut(s) 11, 342, 1076
HpyCH4IV ACGT 1 cut(s) 545
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 90, 393, 614, 992, 1122
HpyF3I CTNAG 2 cut(s) 113, 527
HpySE526I ACGT 1 cut(s) 545
Hsp92I GRCGYC 1 cut(s) 208
HspAI GCGC 4 cut(s) 43, 70, 385, 666
KpnI GGTACC 1 cut(s) 660
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 3 cut(s) 478, 585, 835
LmnI GCTCC 6 cut(s) 28, 40, 376, 504, 992, 1000
Lsp1109I GCAGC 6 cut(s) 366, 405, 460, 973, 1069, 1091
LweI GCATC 4 cut(s) 301, 345, 721, 910
MaeI CTAG 5 cut(s) 275, 497, 728, 899, 954
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 1 cut(s) 11
MalI GATC 3 cut(s) 480, 587, 837
MboI GATC 3 cut(s) 478, 585, 835
MboII GAAGA 4 cut(s) 66, 893, 986, 1053
MfeI CAATTG 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 997
MluCI AATT 4 cut(s) 130, 350, 709, 798
Mph1103I ATGCAT 1 cut(s) 294
MroNI GCCGGC 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 3 cut(s) 623, 804, 1151
MspA1I CMGCKG 2 cut(s) 112, 1082
MspI CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
MspR9I CCNGG 3 cut(s) 38, 402, 685
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 893
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 2 cut(s) 387, 666
MwoI GCNNNNNNNGC 6 cut(s) 42, 90, 393, 614, 992, 1122
NaeI GCCGGC 1 cut(s) 263
NciI CCSGG 2 cut(s) 38, 685
NdeII GATC 3 cut(s) 478, 585, 835
NgoMIV GCCGGC 1 cut(s) 261
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 11
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 5 cut(s) 787, 811, 963, 1035, 1054
PaeI GCATGC 1 cut(s) 787
PaeR7I CTCGAG 1 cut(s) 73
PagI TCATGA 2 cut(s) 148, 582
PaqCI CACCTGC 1 cut(s) 561
PciI ACATGT 4 cut(s) 807, 959, 1031, 1050
PctI GAATGC 1 cut(s) 893
PdiI GCCGGC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 4 cut(s) 57, 169, 579, 895
PflMI CCANNNNNTGG 2 cut(s) 367, 407
PmaCI CACGTG 1 cut(s) 546
PmlI CACGTG 1 cut(s) 546
Ppu21I YACGTR 1 cut(s) 546
PscI ACATGT 4 cut(s) 807, 959, 1031, 1050
PsiI TTATAA 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 997
Psp6I CCWGG 1 cut(s) 400
PspCI CACGTG 1 cut(s) 546
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 658
PspPI GGNCC 2 cut(s) 1022, 1108
PspXI VCTCGAGB 1 cut(s) 73
PvuII CAGCTG 2 cut(s) 112, 1082
RsaI GTAC 1 cut(s) 658
RsaNI GTAC 1 cut(s) 657
SacI GAGCTC 1 cut(s) 997
SaqAI TTAA 3 cut(s) 623, 804, 1151
Sau3AI GATC 3 cut(s) 478, 585, 835
Sau96I GGNCC 2 cut(s) 1022, 1108
ScrFI CCNGG 3 cut(s) 38, 402, 685
SduI GDGCHC 1 cut(s) 997
SfaNI GCATC 4 cut(s) 301, 345, 721, 910
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 1022
SlaI CTCGAG 1 cut(s) 73
SmlI CTYRAG 3 cut(s) 73, 315, 418
SmoI CTYRAG 3 cut(s) 73, 315, 418
SphI GCATGC 1 cut(s) 787
Sse9I AATT 4 cut(s) 130, 350, 709, 798
SspI AATATT 2 cut(s) 637, 853
SspMI CTAG 5 cut(s) 275, 497, 728, 899, 954
SstI GAGCTC 1 cut(s) 997
StyD4I CCNGG 3 cut(s) 36, 400, 683
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 3 cut(s) 11, 342, 1076
TaiI ACGT 1 cut(s) 548
TaqI TCGA 6 cut(s) 52, 74, 267, 1120, 1146, 1163
TasI AATT 4 cut(s) 130, 350, 709, 798
TauI GCSGC 8 cut(s) 48, 93, 160, 374, 385, 598, 608, 1098
TfiI GAWTC 4 cut(s) 57, 169, 579, 895
Tru1I TTAA 3 cut(s) 623, 804, 1151
Tru9I TTAA 3 cut(s) 623, 804, 1151
TscAI CASTG 4 cut(s) 16, 345, 828, 1081
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 6 cut(s) 379, 393, 448, 986, 1079, 1082
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 5 cut(s) 108, 165, 571, 894, 900
TspRI CASTG 4 cut(s) 16, 345, 828, 1081
Van91I CCANNNNNTGG 2 cut(s) 367, 407
VpaK11BI GGWCC 1 cut(s) 1022
XapI RAATTY 1 cut(s) 798
XceI RCATGY 5 cut(s) 787, 811, 963, 1035, 1054
XhoI CTCGAG 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 5 cut(s) 275, 497, 728, 899, 954
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.