Rh5BG301000

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015576
Physical Location & Seq
Forward (+)
38806929 .. 38811238
4310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG301000.1

Sequence Viewer

Length: 837 bp
ATGGAATACAGTGACACAGATACGCCGGAGCAATGCCGGGAGCGCCGCCATCGAAGAATCCTGATGAGGCGCTCGAGGTGGTGCTGTTGCGGCCAGCACTTTCACCGCCAGCTGAGTTCATACCGGACTAATTACACTATGACCACTCATGAAATGCCGCAACACCGGAATCCAATGCCCGATTTCGGCAAGTTTTCACTTATAAGGCGTCGTTCGGAGAGCATTATTGACGATACCTTATTTGTGAAGGAGGACTTCTGCCGGCTCGAACTTCTAGGTGGACAGCCTATGCATTTCTTTGCTGTCTTTGATGCTCAAGGAGACCCTCATGTGTCCACGCTGTGCAAACAATTGATGCACCAATGCGTGGCGGCGGAGCTGCGGCGCGTGTGCAGCACTCCAGGGCTTGGTGAGAACTTGAGCTTGGGTTTGGAAACGGGAAGCGAGCAGCAGGAAGGGGAGGAGGCCAAGTGGCATGATCTGGTGCGAACAGCCCTAGAGAGGAGCTTTCAGAGGATGAACCGTCTCAGGCAACACACTTGCACGTGTGGGAACATTACTGATGATTGCAGGTGTGGATTCATGATCCTCACGCCGCCAGTTGCGGCTGTTGTGGCAATCTTAACAGCTCGGAATATTGTCATTGCTAACTCCGGTACCTCACGCGCCGTCCTTGGTCGTGCCGGGAGTTCTTTACCACTCTTTGATAATTCCAAGGTAACGTCAACTTTTTCTAGAAAAATTTGCTACTCTGAGAAGCATGACAGCTTCCAAGTCGGTCATTTTAACCTTTTATTAGATGCAATTGCTGAAATTTTCATTGCATCACATAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

278

Amino Acids

31.7

Weight (kDa)

8.24

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015576)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24380
malus_domestica MD01G1191100.v1.1
prunus_persica Prupe.2G285600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0043861 RchiOBHm_Chr5g0043871
rosa_laevigata RLG00000034220
rosa_multiflora Rmu_co8273823.1_g000001 Rmu_sc0009227.1_g000016 Rmu_sc0010851.1_g000002
rosa_roxburghii Rroxscaffold_1G00037190
rosa_rugosa Rorug05G0208900
rosa_samantha Rh5AG292900 Rh5BG301000 Rh5CG328600 Rh5DG309900
rosa_wichuraiana Rw5G027160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AarI CACCTGC 1 cut(s) 561
Acc36I ACCTGC 1 cut(s) 561
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccB7I CCANNNNNTGG 2 cut(s) 367, 407
AccII CGCG 2 cut(s) 387, 666
AciI CCGC 9 cut(s) 46, 90, 106, 158, 371, 374, 382, 596, 605
AclWI GGATC 1 cut(s) 580
AcoI YGGCCR 1 cut(s) 91
AcsI RAATTY 2 cut(s) 741, 813
AcvI CACGTG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 208
AdeI CACNNNGTG 1 cut(s) 342
AfaI GTAC 1 cut(s) 658
AfiI CCNNNNNNNGG 4 cut(s) 185, 367, 407, 501
AflIII ACRYGT 1 cut(s) 545
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 7 cut(s) 112, 379, 423, 507, 629, 768, 834
AluI AGCT 7 cut(s) 112, 379, 423, 507, 629, 768, 834
Alw26I GTCTC 2 cut(s) 315, 530
AlwI GGATC 1 cut(s) 580
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 2 cut(s) 91, 465
ApeKI GCWGC 3 cut(s) 379, 393, 448
ApoI RAATTY 2 cut(s) 741, 813
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 4 cut(s) 45, 72, 387, 668
AsuC2I CCSGG 2 cut(s) 38, 685
AsuHPI GGTGA 2 cut(s) 95, 422
AvaI CYCGRG 1 cut(s) 73
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BanI GGYRCC 1 cut(s) 656
BbrPI CACGTG 1 cut(s) 546
BbvI GCAGC 3 cut(s) 366, 405, 460
BccI CCATC 1 cut(s) 57
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 1 cut(s) 402
BcnI CCSGG 2 cut(s) 38, 685
BcoDI GTCTC 2 cut(s) 315, 530
BfaI CTAG 3 cut(s) 275, 497, 735
BfoI RGCGCY 2 cut(s) 46, 73
BfuAI ACCTGC 1 cut(s) 561
Bme1390I CCNGG 3 cut(s) 38, 402, 685
BmeT110I CYCGRG 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 3 cut(s) 38, 402, 685
BmsI GCATC 4 cut(s) 301, 345, 790, 833
BpmI CTGGAG 1 cut(s) 384
BpuEI CTTGAG 2 cut(s) 300, 439
BpuMI CCSGG 2 cut(s) 38, 685
BsaAI YACGTR 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 401, 673, 714
BsaWI WCCGGW 3 cut(s) 123, 165, 653
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 367, 407, 501
Bse118I RCCGGY 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 599
Bse3DI GCAATG 3 cut(s) 38, 642, 819
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 3 cut(s) 401, 673, 714
BseGI GGATG 1 cut(s) 522
BseLI CCNNNNNNNGG 4 cut(s) 185, 367, 407, 501
BseMI GCAATG 3 cut(s) 38, 642, 819
BseMII CTCAG 3 cut(s) 104, 541, 744
BseNI ACTGG 1 cut(s) 599
BseRI GAGGAG 2 cut(s) 476, 517
BseXI GCAGC 3 cut(s) 366, 405, 460
BsgI GTGCAG 1 cut(s) 412
Bsh1236I CGCG 2 cut(s) 387, 666
BshFI GGCC 2 cut(s) 93, 467
BshNI GGYRCC 1 cut(s) 656
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
BslI CCNNNNNNNGG 4 cut(s) 185, 367, 407, 501
BsmAI GTCTC 2 cut(s) 315, 530
BsmBI CGTCTC 1 cut(s) 530
BsnI GGCC 2 cut(s) 93, 467
Bso31I GGTCTC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 73
Bsp143I GATC 2 cut(s) 478, 585
BspACI CCGC 9 cut(s) 46, 90, 106, 158, 371, 374, 382, 596, 605
BspANI GGCC 2 cut(s) 93, 467
BspCNI CTCAG 3 cut(s) 105, 540, 745
BspFNI CGCG 2 cut(s) 387, 666
BspHI TCATGA 2 cut(s) 148, 582
BspLI GGNNCC 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 315
BsrDI GCAATG 3 cut(s) 38, 642, 819
BsrFI RCCGGY 1 cut(s) 261
BsrI ACTGG 1 cut(s) 599
BssAI RCCGGY 1 cut(s) 261
BssECI CCNNGG 3 cut(s) 401, 673, 714
BssMI GATC 2 cut(s) 478, 585
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 2 cut(s) 673, 714
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 11, 524
BstACI GRCGYC 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 4 cut(s) 95, 110, 263, 446
BstDEI CTNAG 3 cut(s) 113, 527, 753
BstF5I GGATG 1 cut(s) 522
BstFNI CGCG 2 cut(s) 387, 666
BstH2I RGCGCY 2 cut(s) 46, 73
BstHHI GCGC 4 cut(s) 45, 72, 387, 668
BstKTI GATC 2 cut(s) 481, 588
BstMAI GTCTC 2 cut(s) 315, 530
BstMBI GATC 2 cut(s) 478, 585
BstMWI GCNNNNNNNGC 4 cut(s) 42, 90, 393, 614
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 3 cut(s) 36, 400, 683
BstUI CGCG 2 cut(s) 387, 666
BstV1I GCAGC 3 cut(s) 366, 405, 460
BsuRI GGCC 2 cut(s) 93, 467
BtsCI GGATG 1 cut(s) 522
BtsIMutI CAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 561
Cac8I GCNNGC 4 cut(s) 95, 110, 263, 446
CciI TCATGA 2 cut(s) 148, 582
CfoI GCGC 4 cut(s) 45, 72, 387, 668
Cfr10I RCCGGY 1 cut(s) 261
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 657
CviAII CATG 5 cut(s) 149, 329, 476, 583, 761
CviQI GTAC 1 cut(s) 657
DdeI CTNAG 3 cut(s) 113, 527, 753
DpnI GATC 2 cut(s) 480, 587
DpnII GATC 2 cut(s) 478, 585
DraIII CACNNNGTG 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 91
EciI GGCGGA 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 673, 714
Eco31I GGTCTC 1 cut(s) 315
Eco72I CACGTG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 673, 714
EcoT22I ATGCAT 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 673, 714
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 5 cut(s) 152, 332, 479, 586, 764
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FatI CATG 5 cut(s) 148, 328, 475, 582, 760
FokI GGATG 1 cut(s) 529
FspBI CTAG 3 cut(s) 275, 497, 735
GlaI GCGC 4 cut(s) 44, 71, 386, 667
GsuI CTGGAG 1 cut(s) 384
HaeII RGCGCY 2 cut(s) 46, 73
HaeIII GGCC 2 cut(s) 93, 467
HapII CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
HgaI GACGC 1 cut(s) 197
HhaI GCGC 4 cut(s) 45, 72, 387, 668
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 5 cut(s) 152, 332, 479, 586, 764
Hin6I GCGC 4 cut(s) 43, 70, 385, 666
HinP1I GCGC 4 cut(s) 43, 70, 385, 666
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HinfI GANTC 3 cut(s) 57, 169, 579
HpaII CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
HphI GGTGA 2 cut(s) 95, 422
Hpy166II GTNNAC 3 cut(s) 281, 336, 726
Hpy188I TCNGA 4 cut(s) 217, 513, 633, 754
Hpy188III TCNNGA 4 cut(s) 61, 149, 583, 735
Hpy8I GTNNAC 3 cut(s) 281, 336, 726
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 2 cut(s) 241, 449
HpyCH4III ACNGT 2 cut(s) 11, 524
HpyCH4IV ACGT 2 cut(s) 545, 722
HpyCH4V TGCA 8 cut(s) 292, 345, 358, 393, 543, 570, 803, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 90, 393, 614
HpyF3I CTNAG 3 cut(s) 113, 527, 753
HpySE526I ACGT 2 cut(s) 545, 722
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 5 cut(s) 152, 332, 479, 586, 764
HspAI GCGC 4 cut(s) 43, 70, 385, 666
KpnI GGTACC 1 cut(s) 660
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 2 cut(s) 478, 585
LmnI GCTCC 4 cut(s) 28, 40, 376, 504
Lsp1109I GCAGC 3 cut(s) 366, 405, 460
LweI GCATC 4 cut(s) 301, 345, 790, 833
MaeI CTAG 3 cut(s) 275, 497, 735
MaeII ACGT 2 cut(s) 545, 722
MaeIII GTNAC 2 cut(s) 11, 718
MalI GATC 2 cut(s) 480, 587
MboI GATC 2 cut(s) 478, 585
MboII GAAGA 1 cut(s) 66
MfeI CAATTG 2 cut(s) 350, 804
MluCI AATT 6 cut(s) 130, 350, 709, 741, 804, 813
Mph1103I ATGCAT 1 cut(s) 294
MroNI GCCGGC 1 cut(s) 261
MseI TTAA 2 cut(s) 623, 786
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 7 cut(s) 26, 37, 124, 166, 262, 654, 684
MspR9I CCNGG 3 cut(s) 38, 402, 685
MunI CAATTG 2 cut(s) 350, 804
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 2 cut(s) 387, 666
MwoI GCNNNNNNNGC 4 cut(s) 42, 90, 393, 614
NaeI GCCGGC 1 cut(s) 263
NciI CCSGG 2 cut(s) 38, 685
NdeII GATC 2 cut(s) 478, 585
NgoMIV GCCGGC 1 cut(s) 261
NlaIII CATG 5 cut(s) 152, 332, 479, 586, 764
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 11
NsiI ATGCAT 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 73
PagI TCATGA 2 cut(s) 148, 582
PaqCI CACCTGC 1 cut(s) 561
PdiI GCCGGC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 57, 169, 579
PflMI CCANNNNNTGG 2 cut(s) 367, 407
PmaCI CACGTG 1 cut(s) 546
PmlI CACGTG 1 cut(s) 546
Ppu21I YACGTR 1 cut(s) 546
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 400
PspCI CACGTG 1 cut(s) 546
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 658
PspXI VCTCGAGB 1 cut(s) 73
PvuII CAGCTG 1 cut(s) 112
RsaI GTAC 1 cut(s) 658
RsaNI GTAC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 623, 786
Sau3AI GATC 2 cut(s) 478, 585
ScrFI CCNGG 3 cut(s) 38, 402, 685
SfaNI GCATC 4 cut(s) 301, 345, 790, 833
Sfr274I CTCGAG 1 cut(s) 73
SlaI CTCGAG 1 cut(s) 73
SmlI CTYRAG 3 cut(s) 73, 315, 418
SmoI CTYRAG 3 cut(s) 73, 315, 418
Sse9I AATT 6 cut(s) 130, 350, 709, 741, 804, 813
SsiI CCGC 9 cut(s) 46, 90, 106, 158, 371, 374, 382, 596, 605
SspI AATATT 1 cut(s) 637
SspMI CTAG 3 cut(s) 275, 497, 735
StyD4I CCNGG 3 cut(s) 36, 400, 683
StyI CCWWGG 2 cut(s) 673, 714
TaaI ACNGT 2 cut(s) 11, 524
TaiI ACGT 2 cut(s) 548, 725
TaqI TCGA 3 cut(s) 52, 74, 267
TaqII GACCGA 1 cut(s) 767
TasI AATT 6 cut(s) 130, 350, 709, 741, 804, 813
TauI GCSGC 7 cut(s) 48, 93, 160, 374, 385, 598, 608
TfiI GAWTC 3 cut(s) 57, 169, 579
Tru1I TTAA 2 cut(s) 623, 786
Tru9I TTAA 2 cut(s) 623, 786
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 3 cut(s) 379, 393, 448
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 5 cut(s) 108, 165, 533, 571, 808
TspRI CASTG 1 cut(s) 16
Van91I CCANNNNNTGG 2 cut(s) 367, 407
XapI RAATTY 2 cut(s) 741, 813
XbaI TCTAGA 1 cut(s) 734
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 3 cut(s) 275, 497, 735
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.