Rw5G027160

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015576
Physical Location & Seq
Forward (+)
39870200 .. 39872247
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027160.1

Sequence Viewer

Length: 1008 bp
ATGCCCGATTTCGGCAAGTTCTCACTTATAAGGCGTCGTTCGGAGAGCATTATTGACGATACCTTATTTGTGAAGGAGGACTTCTGCCGGCTCGAACTTCTAGGTGGACAGCCTATGCATTTCTTTGCTGTCTTTGATGCTCAAGGAGACCCTCATGTGTCCACGCTGTGCAAACAATTGATGCACCAATGCGTGGCGGCGGAGCTGCGGCGCGTGTGCAGCACTCCAGGGCTTGGTGAGAACTTGAGCTTGGGTTTGGAAACGGGAAGCGAGCAGCAGGAAGGGGAGGAGGCCAAGTGGCATGATCTGGTGCGAACAGCCCTAGAGAGGAGCTTTCAGAGGATGAACCGTCTCAGGCAACACACTTGCACGTGTGGGAACATTACTGATGATTGCAGGTGTGGATTCATGATCCTCACGCCGCCAGTTGCGGCTGTTGTGGCAATCTTAACAGCTCGGAATATTGTCATTGCTAACTCCGGTACCTCACGCGCCGTCCTTGGCCGTGCCGGGAGTTCTTTACCACTCTTTGATAATGCCAAGCCTGAAAGACTAGATGCACAAGAAAGGCTTCGTGCAGTAGATGGTCGGGTTTTCCATCACAACGGCATGCGTGTATACGGAATTCTTAATATGTCTCATTCCGCAGTGCAAACAGGGGATCACTACCTTCTGAATATTTCCATCACAGAAAGAGAGGGAAATGAAGATGAATGCTTGATTCTAGCCAATCATGGTATTTGGGATGCTATATCAGATGACATGGCTTGCAGGGTAGCTAGAACATGTCTCACATTTGAAGATGGGGAAGCTGCTAGAGCTCCAGCCAAAGATGGTCGTCTTTATGGTCCAGACACATGTGATCATGAGACGAACATGTTTTTCTCTTCCAAAACCAACAGTGCAGCTGCAATACTCTGCCGCCTCGCTTTGGGCCGTGGCAGTCGAGGCGATATAAGTGTCATTGTTGTCGATTTAATGGTTAGGTCGAAGTCAAGGCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

335

Amino Acids

37.04

Weight (kDa)

6.27

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 20 - 274 4.2e-19 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015576)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24380
malus_domestica MD01G1191100.v1.1
prunus_persica Prupe.2G285600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0043861 RchiOBHm_Chr5g0043871
rosa_laevigata RLG00000034220
rosa_multiflora Rmu_co8273823.1_g000001 Rmu_sc0009227.1_g000016 Rmu_sc0010851.1_g000002
rosa_roxburghii Rroxscaffold_1G00037190
rosa_rugosa Rorug05G0208900
rosa_samantha Rh5AG292900 Rh5BG301000 Rh5CG328600 Rh5DG309900
rosa_wichuraiana Rw5G027160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
AarI CACCTGC 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 387
Acc65I GGTACC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 482
AccB7I CCANNNNNTGG 2 cut(s) 193, 233
AccI GTMKAC 1 cut(s) 618
AccII CGCG 2 cut(s) 213, 492
AciI CCGC 7 cut(s) 197, 200, 208, 422, 431, 645, 922
AclWI GGATC 2 cut(s) 406, 669
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 1 cut(s) 624
AcvI CACGTG 1 cut(s) 372
AcyI GRCGYC 1 cut(s) 34
AdeI CACNNNGTG 1 cut(s) 168
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 5 cut(s) 11, 193, 233, 327, 931
AflIII ACRYGT 4 cut(s) 371, 785, 857, 876
AgsI TTSAA 1 cut(s) 800
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 8 cut(s) 205, 249, 333, 455, 779, 812, 821, 908
AluI AGCT 8 cut(s) 205, 249, 333, 455, 779, 812, 821, 908
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 5 cut(s) 141, 356, 642, 794, 863
AlwI GGATC 2 cut(s) 406, 669
AoxI GGCC 3 cut(s) 291, 502, 934
ApeKI GCWGC 6 cut(s) 205, 219, 274, 812, 905, 908
ApoI RAATTY 1 cut(s) 624
Asp700I GAANNNNTTC 1 cut(s) 570
Asp718I GGTACC 1 cut(s) 482
AspLEI GCGC 2 cut(s) 213, 494
AspS9I GGNCC 2 cut(s) 848, 934
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 848
BaeI ACNNNNGTAYC 2 cut(s) 466, 499
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 823
BbrPI CACGTG 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 6 cut(s) 192, 231, 286, 799, 895, 917
BccI CCATC 5 cut(s) 578, 606, 692, 797, 827
BceAI ACGGC 4 cut(s) 479, 489, 622, 921
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 5 cut(s) 141, 356, 642, 794, 863
BfaI CTAG 6 cut(s) 101, 323, 554, 725, 780, 816
BfuAI ACCTGC 1 cut(s) 387
Bme1390I CCNGG 2 cut(s) 228, 511
Bme18I GGWCC 1 cut(s) 848
BmgT120I GGNCC 2 cut(s) 848, 934
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 2 cut(s) 228, 511
BmsI GCATC 4 cut(s) 127, 171, 547, 736
BpmI CTGGAG 2 cut(s) 210, 807
BpuEI CTTGAG 2 cut(s) 126, 265
BpuMI CCSGG 1 cut(s) 511
BsaAI YACGTR 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 227, 499, 937
BsaWI WCCGGW 1 cut(s) 479
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 193, 233, 327, 931
Bse118I RCCGGY 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 227, 499, 937
BseGI GGATG 2 cut(s) 348, 751
BseLI CCNNNNNNNGG 5 cut(s) 11, 193, 233, 327, 931
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 367
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 2 cut(s) 302, 343
BseXI GCAGC 6 cut(s) 192, 231, 286, 799, 895, 917
BsgI GTGCAG 3 cut(s) 238, 597, 924
Bsh1236I CGCG 2 cut(s) 213, 492
BshFI GGCC 3 cut(s) 293, 504, 936
BshNI GGYRCC 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 3 cut(s) 88, 480, 510
BslI CCNNNNNNNGG 5 cut(s) 11, 193, 233, 327, 931
BsmAI GTCTC 5 cut(s) 141, 356, 642, 794, 863
BsmBI CGTCTC 2 cut(s) 356, 863
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 3 cut(s) 293, 504, 936
Bso31I GGTCTC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 823
Bsp143I GATC 4 cut(s) 304, 411, 661, 862
BspACI CCGC 7 cut(s) 197, 200, 208, 422, 431, 645, 922
BspANI GGCC 3 cut(s) 293, 504, 936
BspCNI CTCAG 1 cut(s) 366
BspFNI CGCG 2 cut(s) 213, 492
BspHI TCATGA 2 cut(s) 408, 865
BspLI GGNNCC 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 387
BspPI GGATC 2 cut(s) 406, 669
BspT107I GGYRCC 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 468
BsrFI RCCGGY 1 cut(s) 87
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 3 cut(s) 227, 499, 937
BssMI GATC 4 cut(s) 304, 411, 661, 862
BssNAI GTATAC 1 cut(s) 619
BssNI GRCGYC 1 cut(s) 34
BssT1I CCWWGG 1 cut(s) 499
Bst1107I GTATAC 1 cut(s) 619
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 350, 902
Bst6I CTCTTC 1 cut(s) 892
BstACI GRCGYC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 89, 272, 611, 769
BstDEI CTNAG 1 cut(s) 353
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 2 cut(s) 348, 751
BstFNI CGCG 2 cut(s) 213, 492
BstHHI GCGC 2 cut(s) 213, 494
BstKTI GATC 4 cut(s) 307, 414, 664, 865
BstMAI GTCTC 5 cut(s) 141, 356, 642, 794, 863
BstMBI GATC 4 cut(s) 304, 411, 661, 862
BstMWI GCNNNNNNNGC 4 cut(s) 219, 440, 818, 948
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 4 cut(s) 613, 789, 861, 880
BstSCI CCNGG 2 cut(s) 226, 509
BstUI CGCG 2 cut(s) 213, 492
BstV1I GCAGC 6 cut(s) 192, 231, 286, 799, 895, 917
BstZ17I GTATAC 1 cut(s) 619
BsuRI GGCC 3 cut(s) 293, 504, 936
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 2 cut(s) 348, 751
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 654, 907
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 4 cut(s) 89, 272, 611, 769
CciI TCATGA 2 cut(s) 408, 865
CfoI GCGC 2 cut(s) 213, 494
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 848, 934
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 483
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 1 cut(s) 353
DpnI GATC 4 cut(s) 306, 413, 663, 864
DpnII GATC 4 cut(s) 304, 411, 661, 862
DraIII CACNNNGTG 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 892
EarI CTCTTC 1 cut(s) 892
EciI GGCGGA 1 cut(s) 215
Ecl136II GAGCTC 1 cut(s) 821
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 823
Eco31I GGTCTC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 848
Eco53kI GAGCTC 1 cut(s) 821
Eco72I CACGTG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 821
EcoRI GAATTC 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 823
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 2 cut(s) 356, 863
FalI AAGNNNNNCTT 4 cut(s) 65, 97, 555, 587
FbaI TGATCA 1 cut(s) 862
FblI GTMKAC 1 cut(s) 618
FokI GGATG 2 cut(s) 355, 758
FriOI GRGCYC 1 cut(s) 823
FspBI CTAG 6 cut(s) 101, 323, 554, 725, 780, 816
GlaI GCGC 2 cut(s) 212, 493
GsuI CTGGAG 2 cut(s) 210, 807
HaeIII GGCC 3 cut(s) 293, 504, 936
HapII CCGG 3 cut(s) 88, 480, 510
HgaI GACGC 1 cut(s) 23
HhaI GCGC 2 cut(s) 213, 494
Hin1I GRCGYC 1 cut(s) 34
Hin6I GCGC 2 cut(s) 211, 492
HinP1I GCGC 2 cut(s) 211, 492
HinfI GANTC 2 cut(s) 405, 721
HpaII CCGG 3 cut(s) 88, 480, 510
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 3 cut(s) 107, 162, 619
Hpy188I TCNGA 5 cut(s) 43, 339, 459, 675, 757
Hpy188III TCNNGA 3 cut(s) 409, 851, 866
Hpy8I GTNNAC 3 cut(s) 107, 162, 619
Hpy99I CGWCG 1 cut(s) 39
HpyAV CCTTC 3 cut(s) 67, 275, 680
HpyCH4III ACNGT 2 cut(s) 350, 902
HpyCH4IV ACGT 1 cut(s) 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 219, 440, 818, 948
HpyF3I CTNAG 1 cut(s) 353
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 1 cut(s) 34
HspAI GCGC 2 cut(s) 211, 492
KpnI GGTACC 1 cut(s) 486
KroI GCCGGC 1 cut(s) 87
KroNI GCCGGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 4 cut(s) 304, 411, 661, 862
LmnI GCTCC 3 cut(s) 202, 330, 826
Lsp1109I GCAGC 6 cut(s) 192, 231, 286, 799, 895, 917
LweI GCATC 4 cut(s) 127, 171, 547, 736
MaeI CTAG 6 cut(s) 101, 323, 554, 725, 780, 816
MaeII ACGT 1 cut(s) 371
MalI GATC 4 cut(s) 306, 413, 663, 864
MboI GATC 4 cut(s) 304, 411, 661, 862
MboII GAAGA 3 cut(s) 719, 812, 879
MfeI CAATTG 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 823
MluCI AATT 2 cut(s) 176, 624
Mph1103I ATGCAT 1 cut(s) 120
MroNI GCCGGC 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 3 cut(s) 449, 630, 977
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 3 cut(s) 88, 480, 510
MspR9I CCNGG 2 cut(s) 228, 511
MunI CAATTG 1 cut(s) 176
Mva1269I GAATGC 1 cut(s) 719
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 2 cut(s) 213, 492
MwoI GCNNNNNNNGC 4 cut(s) 219, 440, 818, 948
NaeI GCCGGC 1 cut(s) 89
NciI CCSGG 1 cut(s) 511
NdeII GATC 4 cut(s) 304, 411, 661, 862
NgoMIV GCCGGC 1 cut(s) 87
NlaIV GGNNCC 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 4 cut(s) 613, 789, 861, 880
PaeI GCATGC 1 cut(s) 613
PagI TCATGA 2 cut(s) 408, 865
PaqCI CACCTGC 1 cut(s) 387
PciI ACATGT 3 cut(s) 785, 857, 876
PctI GAATGC 1 cut(s) 719
PdiI GCCGGC 1 cut(s) 89
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 2 cut(s) 405, 721
PflMI CCANNNNNTGG 2 cut(s) 193, 233
PmaCI CACGTG 1 cut(s) 372
PmlI CACGTG 1 cut(s) 372
Ppu21I YACGTR 1 cut(s) 372
PscI ACATGT 3 cut(s) 785, 857, 876
PsiI TTATAA 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 823
Psp6I CCWGG 1 cut(s) 226
PspCI CACGTG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 2 cut(s) 848, 934
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
SacI GAGCTC 1 cut(s) 823
SaqAI TTAA 3 cut(s) 449, 630, 977
Sau3AI GATC 4 cut(s) 304, 411, 661, 862
Sau96I GGNCC 2 cut(s) 848, 934
ScrFI CCNGG 2 cut(s) 228, 511
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 4 cut(s) 127, 171, 547, 736
SinI GGWCC 1 cut(s) 848
SmlI CTYRAG 2 cut(s) 141, 244
SmoI CTYRAG 2 cut(s) 141, 244
SphI GCATGC 1 cut(s) 613
Sse9I AATT 2 cut(s) 176, 624
SsiI CCGC 7 cut(s) 197, 200, 208, 422, 431, 645, 922
SspI AATATT 2 cut(s) 463, 679
SspMI CTAG 6 cut(s) 101, 323, 554, 725, 780, 816
SstI GAGCTC 1 cut(s) 823
StyD4I CCNGG 2 cut(s) 226, 509
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 2 cut(s) 350, 902
TaiI ACGT 1 cut(s) 374
TaqI TCGA 4 cut(s) 93, 946, 972, 989
TasI AATT 2 cut(s) 176, 624
TauI GCSGC 5 cut(s) 200, 211, 424, 434, 924
TfiI GAWTC 2 cut(s) 405, 721
Tru1I TTAA 3 cut(s) 449, 630, 977
Tru9I TTAA 3 cut(s) 449, 630, 977
TscAI CASTG 2 cut(s) 654, 907
TseI GCWGC 6 cut(s) 205, 219, 274, 812, 905, 908
TspDTI ATGAA 4 cut(s) 359, 397, 720, 726
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 2 cut(s) 654, 907
Van91I CCANNNNNTGG 2 cut(s) 193, 233
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 1 cut(s) 624
XceI RCATGY 4 cut(s) 613, 789, 861, 880
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 6 cut(s) 101, 323, 554, 725, 780, 816
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.