Rh5CG328600

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0015576
Physical Location & Seq
Forward (+)
36714512 .. 36717162
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG328600.1

Sequence Viewer

Length: 1305 bp
ATGGCGCATGCATACCTCTCTCCTTCCACGGGTCACCAAACAACTCATCCATCTACCACCTGCTACTCTAGTACGTTTTCTCTTCTATTTACTCCTCCGCCCCCTCATCTCTCTCCTCGCTACCAACCCAATATGGAGTACTGTGACACAGATACGCCGGAGCAATGCCGGGAGCGCCGCCATCGAAGAATCCAGATGAGGCGCTCGAGGTGGTGCTGTTGCGGCCAGCACTTTCACCGCCAGCTGAGTTCATACCGGACTAATTACACTATGACCACTCATGAAATGCCGCAACACCGGAATCCAATGCCCGATTTCGGCAAGTTTTCGCTTATAAGGCGGCGTTCGGAGAGCATTATTGACGATACCTTATTTGTGAAGGAGGACTTCTGCCGGCTCGAACTTCTAGGTGGACAGCCTATGCATTTCTTTGCTGTCTTTGATGCTCAAGGAGACCCTCATGTGTCCACGCTGTGCAAACAATTGATGCACCAATGCGTGGCGGCGGAGCTGCGGCGCGTGTGCAGCACTCCAGGGCTTGGTGAGAACTTGAGCTTGGGTTTGGAAATGGGAAGCGAGCAGCAGGAAGGGGAGGAGGCCAAGTGGCATGATCTGGTGCGAACAGCTATAGAGAGGAGCTTTCAGAGGATGAACCGTCTCAGGCAACACACTTGCACGTGTGGGAACATTACTGATGATTGCAGGTGTGGATTCATGATCCTCACGCCGCCAGTTGCGGCTGTTGTGGCAATCTTAACAGCTCGGAATATTGTCATTGCTAACTCCGGTACCTCACGCGCCGTCCTTGGCCGTGCCGAGAGTTCTTTACCACTCTTGGATAATTCCAAGCCTGAAAGACTAGATGCACAAGAAAGGCTTCATGCAGTAGATGGTCGAGTTTTCCATCACAATGGCATGCTTGTATATGGAATTCTTAACATGTCTCAATCCCCAGTGCAAACAGGGGATCACCACCTTTTGAATATTTCCATCACAGAAAGAGAGGGAAATGAAGATGAGTGCTTGATTCTAGCCAATCATGGTATTTGGGATGCTATATCAGATGACATGGCTTGCATGGTAGCTAGAACATGTCTCACATTTGAAGATGGGGAAGCTGCTAGAGCGCCAGCCAACAATGGTCGTCTTTATGGTCCAGACACATGCGACGATGGTGAGACGAACATGTTTTTCTCTTCCAAAACCAACAGTGCAGCTGCAATACTCTGCCGCCTGGCGTTGGGCCGTGGTAGTAGAGGCGATATAAGTGTCATAGTTGTTGATTTAATGATTAGGTCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

434

Amino Acids

48.73

Weight (kDa)

6.46

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 123 - 375 3.3e-16 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015576)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24380
malus_domestica MD01G1191100.v1.1
prunus_persica Prupe.2G285600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0043861 RchiOBHm_Chr5g0043871
rosa_laevigata RLG00000034220
rosa_multiflora Rmu_co8273823.1_g000001 Rmu_sc0009227.1_g000016 Rmu_sc0010851.1_g000002
rosa_roxburghii Rroxscaffold_1G00037190
rosa_rugosa Rorug05G0208900
rosa_samantha Rh5AG292900 Rh5BG301000 Rh5CG328600 Rh5DG309900
rosa_wichuraiana Rw5G027160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
AarI CACCTGC 2 cut(s) 68, 693
Acc36I ACCTGC 2 cut(s) 68, 693
Acc65I GGTACC 1 cut(s) 788
AccB1I GGYRCC 1 cut(s) 788
AccB7I CCANNNNNTGG 2 cut(s) 499, 539
AccII CGCG 2 cut(s) 519, 798
AclWI GGATC 2 cut(s) 712, 975
AcoI YGGCCR 2 cut(s) 223, 808
AcsI RAATTY 1 cut(s) 930
AcvI CACGTG 1 cut(s) 678
AdeI CACNNNGTG 1 cut(s) 474
AfaI GTAC 3 cut(s) 73, 140, 790
AfiI CCNNNNNNNGG 5 cut(s) 29, 317, 499, 539, 1240
AflIII ACRYGT 4 cut(s) 677, 939, 1091, 1185
AgsI TTSAA 2 cut(s) 982, 1106
AjnI CCWGG 2 cut(s) 532, 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AluBI AGCT 9 cut(s) 244, 511, 555, 626, 639, 761, 1085, 1118, 1217
AluI AGCT 9 cut(s) 244, 511, 555, 626, 639, 761, 1085, 1118, 1217
Alw26I GTCTC 5 cut(s) 447, 662, 948, 1100, 1172
AlwI GGATC 2 cut(s) 712, 975
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 4 cut(s) 223, 597, 808, 1243
ApeKI GCWGC 6 cut(s) 511, 525, 580, 1118, 1214, 1217
ApoI RAATTY 1 cut(s) 930
Asp700I GAANNNNTTC 1 cut(s) 876
Asp718I GGTACC 1 cut(s) 788
AspLEI GCGC 6 cut(s) 7, 177, 204, 519, 800, 1129
AspS9I GGNCC 2 cut(s) 1154, 1243
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 5 cut(s) 26, 227, 554, 962, 1187
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 1 cut(s) 1154
BaeI ACNNNNGTAYC 2 cut(s) 772, 805
BanI GGYRCC 1 cut(s) 788
BbrPI CACGTG 1 cut(s) 678
BbvI GCAGC 6 cut(s) 498, 537, 592, 1105, 1204, 1226
BccI CCATC 7 cut(s) 58, 189, 884, 912, 998, 1103, 1166
BceAI ACGGC 3 cut(s) 785, 795, 1230
BciT130I CCWGG 2 cut(s) 534, 1235
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 5 cut(s) 447, 662, 948, 1100, 1172
BfaI CTAG 6 cut(s) 69, 407, 860, 1031, 1086, 1122
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 3 cut(s) 178, 205, 1130
BfuAI ACCTGC 2 cut(s) 68, 693
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 3 cut(s) 170, 534, 1235
Bme18I GGWCC 1 cut(s) 1154
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 1154, 1243
BmiI GGNNCC 1 cut(s) 790
BmrFI CCNGG 3 cut(s) 170, 534, 1235
BmrI ACTGGG 1 cut(s) 947
BmsI GCATC 4 cut(s) 433, 477, 853, 1042
BmuI ACTGGG 1 cut(s) 947
BpmI CTGGAG 1 cut(s) 516
BpuEI CTTGAG 2 cut(s) 432, 571
BpuMI CCSGG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 678
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 4 cut(s) 27, 533, 805, 1246
BsaWI WCCGGW 3 cut(s) 255, 297, 785
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 317, 499, 539, 1240
Bse118I RCCGGY 1 cut(s) 393
Bse1I ACTGG 2 cut(s) 731, 953
Bse3DI GCAATG 2 cut(s) 170, 774
BseBI CCWGG 2 cut(s) 534, 1235
BseDI CCNNGG 4 cut(s) 27, 533, 805, 1246
BseGI GGATG 3 cut(s) 46, 654, 1057
BseLI CCNNNNNNNGG 5 cut(s) 29, 317, 499, 539, 1240
BseMI GCAATG 2 cut(s) 170, 774
BseMII CTCAG 2 cut(s) 236, 673
BseNI ACTGG 2 cut(s) 731, 953
BseRI GAGGAG 4 cut(s) 84, 105, 608, 649
BseXI GCAGC 6 cut(s) 498, 537, 592, 1105, 1204, 1226
BsgI GTGCAG 2 cut(s) 544, 1233
Bsh1236I CGCG 2 cut(s) 519, 798
BshFI GGCC 4 cut(s) 225, 599, 810, 1245
BshNI GGYRCC 1 cut(s) 788
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 6 cut(s) 158, 169, 256, 298, 394, 786
BslI CCNNNNNNNGG 5 cut(s) 29, 317, 499, 539, 1240
BsmAI GTCTC 5 cut(s) 447, 662, 948, 1100, 1172
BsmBI CGTCTC 2 cut(s) 662, 1172
BsnI GGCC 4 cut(s) 225, 599, 810, 1245
Bso31I GGTCTC 1 cut(s) 447
BsoBI CYCGRG 1 cut(s) 205
Bsp143I GATC 3 cut(s) 610, 717, 967
BspANI GGCC 4 cut(s) 225, 599, 810, 1245
BspCNI CTCAG 2 cut(s) 237, 672
BspFNI CGCG 2 cut(s) 519, 798
BspHI TCATGA 2 cut(s) 280, 714
BspLI GGNNCC 1 cut(s) 790
BspMI ACCTGC 2 cut(s) 68, 693
BspPI GGATC 2 cut(s) 712, 975
BspT107I GGYRCC 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 447
BsrDI GCAATG 2 cut(s) 170, 774
BsrFI RCCGGY 1 cut(s) 393
BsrI ACTGG 2 cut(s) 731, 953
BssAI RCCGGY 1 cut(s) 393
BssECI CCNNGG 4 cut(s) 27, 533, 805, 1246
BssMI GATC 3 cut(s) 610, 717, 967
BssT1I CCWWGG 1 cut(s) 805
Bst2UI CCWGG 2 cut(s) 534, 1235
Bst4CI ACNGT 3 cut(s) 143, 656, 1211
Bst6I CTCTTC 2 cut(s) 87, 1201
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 8 cut(s) 9, 227, 242, 395, 578, 917, 1075, 1131
BstDEI CTNAG 3 cut(s) 245, 659, 1299
BstDSI CCRYGG 2 cut(s) 27, 1246
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 3 cut(s) 46, 654, 1057
BstFNI CGCG 2 cut(s) 519, 798
BstH2I RGCGCY 3 cut(s) 178, 205, 1130
BstHHI GCGC 6 cut(s) 7, 177, 204, 519, 800, 1129
BstKTI GATC 3 cut(s) 613, 720, 970
BstMAI GTCTC 5 cut(s) 447, 662, 948, 1100, 1172
BstMBI GATC 3 cut(s) 610, 717, 967
BstMWI GCNNNNNNNGC 6 cut(s) 174, 222, 337, 525, 746, 1124
BstNI CCWGG 2 cut(s) 534, 1235
BstNSI RCATGY 6 cut(s) 11, 919, 943, 1095, 1167, 1189
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 3 cut(s) 168, 532, 1233
BstSFI CTRYAG 1 cut(s) 627
BstUI CGCG 2 cut(s) 519, 798
BstV1I GCAGC 6 cut(s) 498, 537, 592, 1105, 1204, 1226
BstXI CCANNNNNNTGG 1 cut(s) 911
BsuRI GGCC 4 cut(s) 225, 599, 810, 1245
BtgI CCRYGG 2 cut(s) 27, 1246
BtsCI GGATG 3 cut(s) 46, 654, 1057
BtsIMutI CAGTG 2 cut(s) 960, 1216
BveI ACCTGC 2 cut(s) 68, 693
Cac8I GCNNGC 8 cut(s) 9, 227, 242, 395, 578, 917, 1075, 1131
CciI TCATGA 2 cut(s) 280, 714
CfoI GCGC 6 cut(s) 7, 177, 204, 519, 800, 1129
Cfr10I RCCGGY 1 cut(s) 393
Cfr13I GGNCC 2 cut(s) 1154, 1243
Csp6I GTAC 3 cut(s) 72, 139, 789
CviQI GTAC 3 cut(s) 72, 139, 789
DdeI CTNAG 3 cut(s) 245, 659, 1299
DpnI GATC 3 cut(s) 612, 719, 969
DpnII GATC 3 cut(s) 610, 717, 967
DraIII CACNNNGTG 1 cut(s) 474
EaeI YGGCCR 2 cut(s) 223, 808
Eam1104I CTCTTC 2 cut(s) 87, 1201
EarI CTCTTC 2 cut(s) 87, 1201
EciI GGCGGA 2 cut(s) 87, 521
Eco130I CCWWGG 1 cut(s) 805
Eco31I GGTCTC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 1154
Eco72I CACGTG 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 205
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 930
EcoRII CCWGG 2 cut(s) 532, 1233
EcoT14I CCWWGG 1 cut(s) 805
EcoT22I ATGCAT 2 cut(s) 13, 426
ErhI CCWWGG 1 cut(s) 805
Esp3I CGTCTC 2 cut(s) 662, 1172
FalI AAGNNNNNCTT 4 cut(s) 371, 403, 861, 893
FokI GGATG 3 cut(s) 33, 661, 1064
FspBI CTAG 6 cut(s) 69, 407, 860, 1031, 1086, 1122
GlaI GCGC 6 cut(s) 6, 176, 203, 518, 799, 1128
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 3 cut(s) 178, 205, 1130
HaeIII GGCC 4 cut(s) 225, 599, 810, 1245
HapII CCGG 6 cut(s) 158, 169, 256, 298, 394, 786
HhaI GCGC 6 cut(s) 7, 177, 204, 519, 800, 1129
Hin6I GCGC 6 cut(s) 5, 175, 202, 517, 798, 1127
HinP1I GCGC 6 cut(s) 5, 175, 202, 517, 798, 1127
HinfI GANTC 4 cut(s) 189, 301, 711, 1027
HpaII CCGG 6 cut(s) 158, 169, 256, 298, 394, 786
HphI GGTGA 5 cut(s) 26, 227, 554, 962, 1187
Hpy166II GTNNAC 2 cut(s) 413, 468
Hpy188I TCNGA 4 cut(s) 349, 645, 765, 1063
Hpy188III TCNNGA 4 cut(s) 193, 281, 715, 1157
Hpy8I GTNNAC 2 cut(s) 413, 468
Hpy99I CGWCG 1 cut(s) 1172
HpyAV CCTTC 3 cut(s) 33, 373, 581
HpyCH4III ACNGT 3 cut(s) 143, 656, 1211
HpyCH4IV ACGT 2 cut(s) 74, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 174, 222, 337, 525, 746, 1124
HpyF3I CTNAG 3 cut(s) 245, 659, 1299
HpySE526I ACGT 2 cut(s) 74, 677
HspAI GCGC 6 cut(s) 5, 175, 202, 517, 798, 1127
KpnI GGTACC 1 cut(s) 792
KroI GCCGGC 1 cut(s) 393
KroNI GCCGGC 1 cut(s) 395
Kzo9I GATC 3 cut(s) 610, 717, 967
LmnI GCTCC 4 cut(s) 160, 172, 508, 636
Lsp1109I GCAGC 6 cut(s) 498, 537, 592, 1105, 1204, 1226
LweI GCATC 4 cut(s) 433, 477, 853, 1042
MaeI CTAG 6 cut(s) 69, 407, 860, 1031, 1086, 1122
MaeII ACGT 2 cut(s) 74, 677
MaeIII GTNAC 2 cut(s) 32, 143
MalI GATC 3 cut(s) 612, 719, 969
MboI GATC 3 cut(s) 610, 717, 967
MboII GAAGA 5 cut(s) 74, 198, 1025, 1118, 1188
MfeI CAATTG 1 cut(s) 482
MluCI AATT 4 cut(s) 262, 482, 841, 930
Mph1103I ATGCAT 2 cut(s) 13, 426
MroNI GCCGGC 1 cut(s) 393
MroXI GAANNNNTTC 1 cut(s) 876
MseI TTAA 3 cut(s) 755, 936, 1286
MslI CAYNNNNRTG 1 cut(s) 909
MspA1I CMGCKG 2 cut(s) 244, 1217
MspI CCGG 6 cut(s) 158, 169, 256, 298, 394, 786
MspR9I CCNGG 3 cut(s) 170, 534, 1235
MunI CAATTG 1 cut(s) 482
MvaI CCWGG 2 cut(s) 534, 1235
MvnI CGCG 2 cut(s) 519, 798
MwoI GCNNNNNNNGC 6 cut(s) 174, 222, 337, 525, 746, 1124
NaeI GCCGGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 170
NdeII GATC 3 cut(s) 610, 717, 967
NgoMIV GCCGGC 1 cut(s) 393
NlaIV GGNNCC 1 cut(s) 790
NmeAIII GCCGAG 1 cut(s) 841
NmuCI GTSAC 2 cut(s) 32, 143
NsiI ATGCAT 2 cut(s) 13, 426
NspI RCATGY 6 cut(s) 11, 919, 943, 1095, 1167, 1189
PaeI GCATGC 2 cut(s) 11, 919
PaeR7I CTCGAG 1 cut(s) 205
PagI TCATGA 2 cut(s) 280, 714
PaqCI CACCTGC 2 cut(s) 68, 693
PciI ACATGT 3 cut(s) 939, 1091, 1185
PdiI GCCGGC 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 4 cut(s) 189, 301, 711, 1027
PflMI CCANNNNNTGG 2 cut(s) 499, 539
PmaCI CACGTG 1 cut(s) 678
PmlI CACGTG 1 cut(s) 678
Ppu21I YACGTR 1 cut(s) 678
PscI ACATGT 3 cut(s) 939, 1091, 1185
PsiI TTATAA 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 532, 1233
PspCI CACGTG 1 cut(s) 678
PspEI GGTNACC 1 cut(s) 32
PspGI CCWGG 2 cut(s) 532, 1233
PspN4I GGNNCC 1 cut(s) 790
PspPI GGNCC 2 cut(s) 1154, 1243
PspXI VCTCGAGB 1 cut(s) 205
PvuII CAGCTG 2 cut(s) 244, 1217
RsaI GTAC 3 cut(s) 73, 140, 790
RsaNI GTAC 3 cut(s) 72, 139, 789
RseI CAYNNNNRTG 1 cut(s) 909
SaqAI TTAA 3 cut(s) 755, 936, 1286
Sau3AI GATC 3 cut(s) 610, 717, 967
Sau96I GGNCC 2 cut(s) 1154, 1243
ScaI AGTACT 1 cut(s) 140
ScrFI CCNGG 3 cut(s) 170, 534, 1235
SfaNI GCATC 4 cut(s) 433, 477, 853, 1042
SfcI CTRYAG 1 cut(s) 627
Sfr274I CTCGAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 1154
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 909
SmlI CTYRAG 3 cut(s) 205, 447, 550
SmoI CTYRAG 3 cut(s) 205, 447, 550
SphI GCATGC 2 cut(s) 11, 919
Sse9I AATT 4 cut(s) 262, 482, 841, 930
SspI AATATT 2 cut(s) 769, 985
SspMI CTAG 6 cut(s) 69, 407, 860, 1031, 1086, 1122
StyD4I CCNGG 3 cut(s) 168, 532, 1233
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 3 cut(s) 143, 656, 1211
TaiI ACGT 2 cut(s) 77, 680
TaqI TCGA 4 cut(s) 184, 206, 399, 895
TasI AATT 4 cut(s) 262, 482, 841, 930
TatI WGTACW 1 cut(s) 138
TauI GCSGC 9 cut(s) 180, 225, 292, 343, 506, 517, 730, 740, 1233
TfiI GAWTC 4 cut(s) 189, 301, 711, 1027
Tru1I TTAA 3 cut(s) 755, 936, 1286
Tru9I TTAA 3 cut(s) 755, 936, 1286
TscAI CASTG 2 cut(s) 960, 1216
TseFI GTSAC 2 cut(s) 32, 143
TseI GCWGC 6 cut(s) 511, 525, 580, 1118, 1214, 1217
Tsp45I GTSAC 2 cut(s) 32, 143
TspDTI ATGAA 6 cut(s) 240, 297, 665, 703, 869, 1026
TspRI CASTG 2 cut(s) 960, 1216
Van91I CCANNNNNTGG 2 cut(s) 499, 539
VpaK11BI GGWCC 1 cut(s) 1154
XapI RAATTY 1 cut(s) 930
XceI RCATGY 6 cut(s) 11, 919, 943, 1095, 1167, 1189
XhoI CTCGAG 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 6 cut(s) 69, 407, 860, 1031, 1086, 1122
ZrmI AGTACT 1 cut(s) 140
Zsp2I ATGCAT 2 cut(s) 13, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.