Rh5AG329800

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010544
Physical Location & Seq
Forward (+)
50370424 .. 50373945
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG329800.1

Sequence Viewer

Length: 555 bp
ATGGGGAACTTGTGCTTTAAACCAGCAAAGTGTGCTCATGCTTCTTCCAACAACTTCCCTGTCCCTCCTGGAGGTACAAAATACAATGGGAAGGAACAGCAGGATTTAGCATCCTCCAACCAGGACAGACCATTGGGTGGTCCCAATACTAAAGCTCTAATAGCACCCAATGCAGCTGATACATGTGTTCCTAGCACCCTTAAGTCCTTTAGTTTCATTGATCTCAAGAATGCTACTAGGAACTTCCGCCCAGATAGTCTACTTGGAGAGGGAGGGTTTGGGTGGGTCTTCAAAGGCTGGATTGATGCAAACACCTTGGCTCCCACTAGACCAGGAACTGGCTTGGTGGTGGCTATCAAGAGGCTCAAGCAAGAAAGCTTTCAAGGTCACAAGGAATGGCTTGATTTCAGTGCAAAGCTATCAGATTTTGGTTTAGCAAGGGACGGTCCTACTGGAGACAACACTCATGTTTCAACCAGAGTCGTGGGAACTAGAGGCTATGCTGCACCAGAATATGTTGCTACAGGTGACTGTACCTTTCAATTGCATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

184

Amino Acids

19.71

Weight (kDa)

8.93

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 137, 338
AccI GTMKAC 1 cut(s) 259
AciI CCGC 1 cut(s) 247
AfaI GTAC 2 cut(s) 76, 535
AfiI CCNNNNNNNGG 3 cut(s) 71, 137, 338
AflII CTTAAG 1 cut(s) 200
AflIII ACRYGT 1 cut(s) 182
AgsI TTSAA 4 cut(s) 292, 383, 474, 542
AjnI CCWGG 3 cut(s) 67, 120, 331
AluBI AGCT 4 cut(s) 155, 176, 378, 418
AluI AGCT 4 cut(s) 155, 176, 378, 418
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 450
AlwNI CAGNNNCTG 1 cut(s) 338
ApeKI GCWGC 2 cut(s) 173, 503
Asp700I GAANNNNTTC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 140, 446
AsuHPI GGTGA 1 cut(s) 539
AvaII GGWCC 2 cut(s) 140, 446
BaeI ACNNNNGTAYC 2 cut(s) 171, 204
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 185, 490
BciT130I CCWGG 3 cut(s) 69, 122, 333
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 4 cut(s) 192, 237, 327, 492
BfmI CTRYAG 1 cut(s) 522
BfrI CTTAAG 1 cut(s) 200
BisI GCNGC 2 cut(s) 174, 504
BlsI GCNGC 2 cut(s) 175, 505
Bme1390I CCNGG 3 cut(s) 69, 122, 333
Bme18I GGWCC 2 cut(s) 140, 446
BmgT120I GGNCC 2 cut(s) 140, 446
BmiI GGNNCC 2 cut(s) 142, 321
BmrFI CCNGG 3 cut(s) 69, 122, 333
BmsI GCATC 2 cut(s) 119, 295
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 2 cut(s) 90, 474
BpuEI CTTGAG 2 cut(s) 209, 350
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 137, 338
Bse1I ACTGG 2 cut(s) 343, 457
BseBI CCWGG 3 cut(s) 69, 122, 333
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 2 cut(s) 110, 547
BseLI CCNNNNNNNGG 3 cut(s) 71, 137, 338
BseNI ACTGG 2 cut(s) 343, 457
BseXI GCAGC 2 cut(s) 185, 490
BsgI GTGCAG 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 37
BslFI GGGAC 3 cut(s) 47, 126, 455
BslI CCNNNNNNNGG 3 cut(s) 71, 137, 338
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 3 cut(s) 47, 126, 455
BsmI GAATGC 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 142, 321
BspTI CTTAAG 1 cut(s) 200
BsrI ACTGG 2 cut(s) 343, 457
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 69, 122, 333
Bst4CI ACNGT 2 cut(s) 446, 533
BstAFI CTTAAG 1 cut(s) 200
BstAPI GCANNNNNTGC 2 cut(s) 32, 170
BstENI CCTNNNNNAGG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 110, 547
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 32, 161, 170
BstNI CCWGG 3 cut(s) 69, 122, 333
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 3 cut(s) 67, 120, 331
BstSFI CTRYAG 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 185, 490
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 484
BtsCI GGATG 2 cut(s) 110, 547
BtsIMutI CAGTG 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 140, 446
Csp6I GTAC 2 cut(s) 75, 534
CviAII CATG 3 cut(s) 38, 183, 467
CviQI GTAC 2 cut(s) 75, 534
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
DraI TTTAAA 1 cut(s) 19
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 140, 446
EcoNI CCTNNNNNAGG 1 cut(s) 69
EcoRII CCWGG 3 cut(s) 67, 120, 331
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 3 cut(s) 41, 186, 470
FaiI YATR 5 cut(s) 39, 184, 468, 501, 516
FaqI GGGAC 3 cut(s) 47, 126, 455
FatI CATG 3 cut(s) 37, 182, 466
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 174, 504
FokI GGATG 2 cut(s) 97, 534
Fsp4HI GCNGC 2 cut(s) 174, 504
FspBI CTAG 4 cut(s) 192, 237, 327, 492
GluI GCNGC 2 cut(s) 174, 504
GsuI CTGGAG 2 cut(s) 90, 474
Hin1II CATG 3 cut(s) 41, 186, 470
HindIII AAGCTT 1 cut(s) 376
HinfI GANTC 1 cut(s) 480
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 2 cut(s) 226, 358
Hpy8I GTNNAC 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 2 cut(s) 446, 533
HpyCH4V TGCA 5 cut(s) 173, 308, 413, 506, 547
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 161, 170
Hsp92II CATG 3 cut(s) 41, 186, 470
Kzo9I GATC 1 cut(s) 220
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 2 cut(s) 185, 490
LweI GCATC 2 cut(s) 119, 295
MaeI CTAG 4 cut(s) 192, 237, 327, 492
MaeIII GTNAC 2 cut(s) 386, 527
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 2 cut(s) 36, 280
MfeI CAATTG 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 1 cut(s) 542
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 2 cut(s) 72, 141
MnlI CCTC 7 cut(s) 65, 75, 124, 262, 266, 354, 488
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 2 cut(s) 18, 201
MspA1I CMGCKG 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 69, 122, 333
MunI CAATTG 1 cut(s) 542
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 3 cut(s) 69, 122, 333
MwoI GCNNNNNNNGC 3 cut(s) 32, 161, 170
NdeII GATC 1 cut(s) 220
NlaIII CATG 3 cut(s) 41, 186, 470
NlaIV GGNNCC 2 cut(s) 142, 321
NmuCI GTSAC 2 cut(s) 386, 527
NspI RCATGY 1 cut(s) 186
PciI ACATGT 1 cut(s) 182
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 378
PflMI CCANNNNNTGG 2 cut(s) 137, 338
PfoI TCCNGGA 1 cut(s) 67
PkrI GCNGC 2 cut(s) 175, 505
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PscI ACATGT 1 cut(s) 182
Psp6I CCWGG 3 cut(s) 67, 120, 331
PspGI CCWGG 3 cut(s) 67, 120, 331
PspN4I GGNNCC 2 cut(s) 142, 321
PspPI GGNCC 2 cut(s) 140, 446
PstNI CAGNNNCTG 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 2 cut(s) 76, 535
RsaNI GTAC 2 cut(s) 75, 534
SaqAI TTAA 2 cut(s) 18, 201
SatI GCNGC 2 cut(s) 174, 504
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 2 cut(s) 140, 446
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 3 cut(s) 69, 122, 333
SduI GDGCHC 1 cut(s) 37
SetI ASST 9 cut(s) 76, 157, 178, 317, 380, 388, 420, 529, 539
SfaNI GCATC 2 cut(s) 119, 295
SfcI CTRYAG 1 cut(s) 522
SinI GGWCC 2 cut(s) 140, 446
SmlI CTYRAG 3 cut(s) 200, 224, 365
SmoI CTYRAG 3 cut(s) 200, 224, 365
Sse9I AATT 1 cut(s) 542
SsiI CCGC 1 cut(s) 247
SspMI CTAG 4 cut(s) 192, 237, 327, 492
StyD4I CCNGG 3 cut(s) 67, 120, 331
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 2 cut(s) 446, 533
TasI AATT 1 cut(s) 542
Tru1I TTAA 2 cut(s) 18, 201
Tru9I TTAA 2 cut(s) 18, 201
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 2 cut(s) 386, 527
TseI GCWGC 2 cut(s) 173, 503
Tsp45I GTSAC 2 cut(s) 386, 527
TspDTI ATGAA 1 cut(s) 205
TspRI CASTG 1 cut(s) 415
Van91I CCANNNNNTGG 2 cut(s) 137, 338
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 2 cut(s) 140, 446
XagI CCTNNNNNAGG 1 cut(s) 69
XceI RCATGY 1 cut(s) 186
XmiI GTMKAC 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 4 cut(s) 192, 237, 327, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.