Rorug05G0256800

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000254
Physical Location & Seq
Forward (+)
28858312 .. 28860196
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0256800.1

Sequence Viewer

Length: 474 bp
ATGTCCTTCTTCACACTACTCCTCTCATGGGCTCTCCTCACCATCCTTCTCTCTCTACCCCTTTCTCTCTCCTCTTCCTCCATCCACGACCTCCTCCGCTCGCAGGGCCTGCCAGCTGGTCTTCTCCCGAAGGAGGTCAAGTCCTACTCACTCTCCGACGACGGCTTCCTCCAAGTCTTCCTCGACGCTCCGTGCCTCACAAAATTCGAGAACCGGGTTTTCTTTGACAGCGTCGTTAGGGCCAACCTCAGCTACGGAAGTCTCATCGGAGTTGAGGGTTTGACTCAGGAAGAGCTCTTTCTGTGGCTGCCGGTGAAGGACATCATCGTTGATGATCCGGGATCAGGTCTCATACTTTTCGACATTGGAGTCGCTCATAAACAGCTTTCTCTGTCGCTCTTTGAAGATCCTCCTTCCTGTGTATTGAAGAATCATGTGAAGATGCTGAAAAAACGGTTTGAAGCTCTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

157

Amino Acids

17.45

Weight (kDa)

5.54

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 135 3.3e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccBSI CCGCTC 1 cut(s) 99
AciI CCGC 1 cut(s) 97
AclWI GGATC 3 cut(s) 329, 349, 401
AcsI RAATTY 1 cut(s) 203
AfiI CCNNNNNNNGG 4 cut(s) 28, 103, 133, 344
AgsI TTSAA 3 cut(s) 404, 427, 461
AluBI AGCT 5 cut(s) 116, 252, 295, 385, 464
AluI AGCT 5 cut(s) 116, 252, 295, 385, 464
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 266, 353
AlwI GGATC 3 cut(s) 329, 349, 401
AlwNI CAGNNNCTG 1 cut(s) 109
AoxI GGCC 2 cut(s) 106, 240
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 106, 240
AsuC2I CCSGG 2 cut(s) 215, 339
AsuHPI GGTGA 2 cut(s) 31, 325
BanII GRGCYC 2 cut(s) 34, 297
BbsI GAAGAC 2 cut(s) 113, 169
Bbv12I GWGCWC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 50, 89
BceAI ACGGC 1 cut(s) 178
BcnI CCSGG 2 cut(s) 215, 339
BcoDI GTCTC 2 cut(s) 266, 353
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 2 cut(s) 215, 339
BmgT120I GGNCC 2 cut(s) 106, 240
BmrFI CCNGG 2 cut(s) 215, 339
BmsI GCATC 1 cut(s) 432
BpiI GAAGAC 2 cut(s) 113, 169
Bpu10I CCTNAGC 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 215, 339
BsaI GGTCTC 1 cut(s) 353
BsaXI ACNNNNNCTCC 4 cut(s) 125, 137, 155, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 103, 133, 344
Bse118I RCCGGY 1 cut(s) 310
BseGI GGATG 2 cut(s) 42, 81
BseLI CCNNNNNNNGG 4 cut(s) 28, 103, 133, 344
BseMII CTCAG 3 cut(s) 262, 299, 458
BseRI GAGGAG 4 cut(s) 11, 26, 61, 83
BseXI GCAGC 1 cut(s) 294
BshFI GGCC 2 cut(s) 108, 242
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 3 cut(s) 214, 311, 338
BslI CCNNNNNNNGG 4 cut(s) 28, 103, 133, 344
BsmAI GTCTC 2 cut(s) 266, 353
BsnI GGCC 2 cut(s) 108, 242
Bso31I GGTCTC 1 cut(s) 353
Bsp1286I GDGCHC 2 cut(s) 34, 297
Bsp143I GATC 3 cut(s) 334, 341, 406
BspACI CCGC 1 cut(s) 97
BspANI GGCC 2 cut(s) 108, 242
BspCNI CTCAG 3 cut(s) 261, 298, 459
BspPI GGATC 3 cut(s) 329, 349, 401
BspQI GCTCTTC 1 cut(s) 285
BspTNI GGTCTC 1 cut(s) 353
BsrBI CCGCTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 310
BssMI GATC 3 cut(s) 334, 341, 406
Bst4CI ACNGT 1 cut(s) 456
Bst6I CTCTTC 2 cut(s) 79, 285
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 101, 110, 114
BstDEI CTNAG 3 cut(s) 248, 285, 467
BstF5I GGATG 2 cut(s) 42, 81
BstKTI GATC 3 cut(s) 337, 344, 409
BstMAI GTCTC 2 cut(s) 266, 353
BstMBI GATC 3 cut(s) 334, 341, 406
BstMWI GCNNNNNNNGC 2 cut(s) 105, 109
BstSCI CCNGG 2 cut(s) 213, 337
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 2 cut(s) 113, 169
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 2 cut(s) 108, 242
BtsCI GGATG 2 cut(s) 42, 81
Cac8I GCNNGC 3 cut(s) 101, 110, 114
CaiI CAGNNNCTG 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 106, 240
CseI GACGC 2 cut(s) 194, 220
CviAII CATG 2 cut(s) 27, 434
DdeI CTNAG 3 cut(s) 248, 285, 467
DpnI GATC 3 cut(s) 336, 343, 408
DpnII GATC 3 cut(s) 334, 341, 406
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 79, 285
EarI CTCTTC 2 cut(s) 79, 285
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 34, 297
Eco31I GGTCTC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 106
EcoT38I GRGCYC 2 cut(s) 34, 297
FaeI CATG 2 cut(s) 30, 437
FaiI YATR 4 cut(s) 28, 353, 378, 435
FatI CATG 2 cut(s) 26, 433
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 2 cut(s) 29, 68
FriOI GRGCYC 2 cut(s) 34, 297
Fsp4HI GCNGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 108, 242
HapII CCGG 3 cut(s) 214, 311, 338
HgaI GACGC 2 cut(s) 194, 220
Hin1II CATG 2 cut(s) 30, 437
HinfI GANTC 3 cut(s) 283, 369, 430
HpaII CCGG 3 cut(s) 214, 311, 338
HphI GGTGA 2 cut(s) 31, 325
Hpy188I TCNGA 3 cut(s) 157, 269, 468
Hpy188III TCNNGA 3 cut(s) 127, 208, 287
Hpy99I CGWCG 4 cut(s) 161, 164, 188, 236
HpyAV CCTTC 5 cut(s) 16, 56, 124, 310, 423
HpyCH4III ACNGT 1 cut(s) 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 109
HpyF3I CTNAG 3 cut(s) 248, 285, 467
Hsp92II CATG 2 cut(s) 30, 437
Kzo9I GATC 3 cut(s) 334, 341, 406
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 1 cut(s) 432
MalI GATC 3 cut(s) 336, 343, 408
MbiI CCGCTC 1 cut(s) 99
MboI GATC 3 cut(s) 334, 341, 406
MboII GAAGA 7 cut(s) 66, 113, 169, 302, 416, 439, 451
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 34, 297
MluCI AATT 1 cut(s) 203
MlyI GAGTC 2 cut(s) 277, 378
MmeI TCCRAC 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 3 cut(s) 214, 311, 338
MspR9I CCNGG 2 cut(s) 215, 339
MwoI GCNNNNNNNGC 2 cut(s) 105, 109
NciI CCSGG 2 cut(s) 215, 339
NdeII GATC 3 cut(s) 334, 341, 406
NlaIII CATG 2 cut(s) 30, 437
PciSI GCTCTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 430
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 1 cut(s) 309
PleI GAGTC 2 cut(s) 277, 377
PpsI GAGTC 2 cut(s) 277, 377
Psp124BI GAGCTC 1 cut(s) 297
PspPI GGNCC 2 cut(s) 106, 240
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 406
PsyI GACNNNGTC 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 116
SacI GAGCTC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 285
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 3 cut(s) 334, 341, 406
Sau96I GGNCC 2 cut(s) 106, 240
SchI GAGTC 2 cut(s) 277, 378
ScrFI CCNGG 2 cut(s) 215, 339
SduI GDGCHC 2 cut(s) 34, 297
SetI ASST 9 cut(s) 93, 118, 138, 249, 254, 297, 349, 387, 466
SfaNI GCATC 1 cut(s) 432
Sse9I AATT 1 cut(s) 203
SsiI CCGC 1 cut(s) 97
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 213, 337
TaaI ACNGT 1 cut(s) 456
TaqI TCGA 3 cut(s) 183, 207, 360
TasI AATT 1 cut(s) 203
TfiI GAWTC 1 cut(s) 430
TseI GCWGC 1 cut(s) 307
TspGWI ACGGA 2 cut(s) 180, 270
Tth111I GACNNNGTC 1 cut(s) 230
XapI RAATTY 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.