FvH4_2g12671

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000254
Physical Location & Seq
Forward (+)
11141704 .. 11149599
7896 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12671.t1

Sequence Viewer

Length: 849 bp
ATGGCGCAGTGGCTCTTGGGTGGTGACGGCACGTTGCCTAGGTTGTGTGGCGACGGTGTAGCGCATCTGGTCGTTTTAGATCAGGGTGGTGTTGAAGCTACGGAGGAGACTGGAGTGGAAATGAAGAAATACCTGCACGCTAGTTCTATTTTTCAAGAAAATTATAAGAAGCCTTCAAACTGCTCCCACACTTGGTCCGGTATTGTGTTTGCTGCTAAACTTGTTAATAGAGGACTTCAATGGAGAAATGGGAATGGTCGTGCTGCTAAATTCTGGGTTGACAAATGGTCACAATGTGGTATTTTAGCTGATTTAGCATTGGATTGTGCCAGTATTGATTTGAATGCTATTGTGAAGGATTTCTGGGTTGACAAAATGACAATATCATTTGGGGAGGAACCCCTTCTGGAAATTTTACAATTAAGTTTGCTTATAAGCTTCTCTATGATGGTCATGGCTTTGAAATTATCCCTGGCTGAAGAACTGTGGAGATGCAAGAATATTTTTGAGGAAAGATTCCATTACCCTTACAATGCTGCTGATATTATTCTTAACTATGCTTCTGCTAAGTCCAAAGTGCAAATGATAGGGCATGTGGAGGCTTTATCATGGTTTAAACCTGCTGTTGGATTTCACAAATTAAATGTGGATGGTTCAAGGACCAAGTCTGGTGAGATAGGTGCTGGAGGAGTTATAAAGGATAGCCGTGGCTGTTGGAGTGGTGGTTTCATGATTAATATTGGTACTGGAGAAGTTTTACAAGCTGAGGCCTGGGGTCTTTTTAAGGGCATTCAAATGGATTTGAACATAAAGATTTCTAAGTCAGAGATCAATGTTGTAGCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.09

Weight (kDa)

6.32

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 165, 434, 695
Acc36I ACCTGC 2 cut(s) 141, 628
AcsI RAATTY 2 cut(s) 269, 411
AcuI CTGAAG 1 cut(s) 498
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 1 cut(s) 745
AfiI CCNNNNNNNGG 2 cut(s) 192, 626
AgsI TTSAA 9 cut(s) 95, 155, 177, 239, 343, 463, 657, 794, 805
AhdI GACNNNNNGTC 1 cut(s) 286
AjnI CCWGG 2 cut(s) 471, 770
AluBI AGCT 4 cut(s) 98, 308, 438, 764
AluI AGCT 4 cut(s) 98, 308, 438, 764
Alw26I GTCTC 1 cut(s) 101
AoxI GGCC 1 cut(s) 768
ApeKI GCWGC 3 cut(s) 212, 263, 536
ApoI RAATTY 2 cut(s) 269, 411
AseI ATTAAT 1 cut(s) 735
Asp700I GAANNNNTTC 2 cut(s) 359, 402
AspA2I CCTAGG 1 cut(s) 38
AspLEI GCGC 2 cut(s) 7, 64
AspS9I GGNCC 2 cut(s) 195, 660
AsuHPI GGTGA 2 cut(s) 35, 683
AvaII GGWCC 2 cut(s) 195, 660
AvrII CCTAGG 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 3 cut(s) 199, 250, 523
BccI CCATC 2 cut(s) 442, 644
BceAI ACGGC 2 cut(s) 43, 690
BciT130I CCWGG 2 cut(s) 473, 772
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 39, 141
BfuAI ACCTGC 2 cut(s) 141, 628
BisI GCNGC 3 cut(s) 213, 264, 537
BlnI CCTAGG 1 cut(s) 38
BlsI GCNGC 3 cut(s) 214, 265, 538
Bme1390I CCNGG 2 cut(s) 473, 772
Bme18I GGWCC 2 cut(s) 195, 660
BmeRI GACNNNNNGTC 1 cut(s) 286
BmgT120I GGNCC 2 cut(s) 195, 660
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 2 cut(s) 473, 772
BmsI GCATC 2 cut(s) 73, 482
BpmI CTGGAG 3 cut(s) 132, 705, 768
Bpu10I CCTNAGC 1 cut(s) 765
BsaJI CCNNGG 4 cut(s) 38, 471, 706, 771
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 709, 739
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 626
Bse1I ACTGG 3 cut(s) 115, 330, 751
BseBI CCWGG 2 cut(s) 473, 772
BseDI CCNNGG 4 cut(s) 38, 471, 706, 771
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 192, 626
BseMII CTCAG 1 cut(s) 756
BseNI ACTGG 3 cut(s) 115, 330, 751
BseRI GAGGAG 2 cut(s) 119, 702
BseXI GCAGC 3 cut(s) 199, 250, 523
BsgI GTGCAG 1 cut(s) 119
BshFI GGCC 1 cut(s) 770
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 2 cut(s) 192, 626
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 2 cut(s) 349, 789
BsnI GGCC 1 cut(s) 770
Bsp143I GATC 2 cut(s) 79, 828
BspANI GGCC 1 cut(s) 770
BspCNI CTCAG 1 cut(s) 757
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 1 cut(s) 399
BspMI ACCTGC 2 cut(s) 141, 628
BsrI ACTGG 3 cut(s) 115, 330, 751
BssECI CCNNGG 4 cut(s) 38, 471, 706, 771
BssMI GATC 2 cut(s) 79, 828
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 473, 772
Bst4CI ACNGT 2 cut(s) 56, 486
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 567, 765, 819
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 1 cut(s) 655
BstHHI GCGC 2 cut(s) 7, 64
BstKTI GATC 2 cut(s) 82, 831
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 79, 828
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 2 cut(s) 473, 772
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 471, 770
BstV1I GCAGC 3 cut(s) 199, 250, 523
BsuRI GGCC 1 cut(s) 770
BtgI CCRYGG 1 cut(s) 706
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 1 cut(s) 14
BveI ACCTGC 2 cut(s) 141, 628
Cac8I GCNNGC 1 cut(s) 138
CciI TCATGA 1 cut(s) 729
CfoI GCGC 2 cut(s) 7, 64
Cfr13I GGNCC 2 cut(s) 195, 660
Csp6I GTAC 1 cut(s) 744
CviAII CATG 4 cut(s) 454, 593, 609, 730
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 3 cut(s) 567, 765, 819
DpnI GATC 2 cut(s) 81, 830
DpnII GATC 2 cut(s) 79, 828
DraI TTTAAA 1 cut(s) 616
DraIII CACNNNGTG 1 cut(s) 296
DriI GACNNNNNGTC 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 38
Eco147I AGGCCT 1 cut(s) 770
Eco47I GGWCC 2 cut(s) 195, 660
Eco57I CTGAAG 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 471, 770
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 4 cut(s) 457, 596, 612, 733
FatI CATG 4 cut(s) 453, 592, 608, 729
Fnu4HI GCNGC 3 cut(s) 213, 264, 537
FokI GGATG 1 cut(s) 662
Fsp4HI GCNGC 3 cut(s) 213, 264, 537
FspBI CTAG 2 cut(s) 39, 141
GlaI GCGC 2 cut(s) 6, 63
GluI GCNGC 3 cut(s) 213, 264, 537
GsuI CTGGAG 3 cut(s) 132, 705, 768
HaeIII GGCC 1 cut(s) 770
HapII CCGG 1 cut(s) 198
HhaI GCGC 2 cut(s) 7, 64
Hin1II CATG 4 cut(s) 457, 596, 612, 733
Hin6I GCGC 2 cut(s) 5, 62
HinP1I GCGC 2 cut(s) 5, 62
HincII GTYRAC 2 cut(s) 280, 370
HindII GTYRAC 2 cut(s) 280, 370
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 1 cut(s) 516
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 35, 683
Hpy166II GTNNAC 2 cut(s) 280, 370
Hpy188I TCNGA 1 cut(s) 826
Hpy188III TCNNGA 3 cut(s) 155, 407, 730
Hpy8I GTNNAC 2 cut(s) 280, 370
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 3 cut(s) 183, 349, 413
HpyCH4III ACNGT 2 cut(s) 56, 486
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 3 cut(s) 136, 495, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 3 cut(s) 567, 765, 819
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 4 cut(s) 457, 596, 612, 733
HspAI GCGC 2 cut(s) 5, 62
Kzo9I GATC 2 cut(s) 79, 828
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 3 cut(s) 199, 250, 523
LweI GCATC 2 cut(s) 73, 482
MaeI CTAG 2 cut(s) 39, 141
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 23, 288
MalI GATC 2 cut(s) 81, 830
MboI GATC 2 cut(s) 79, 828
MboII GAAGA 2 cut(s) 136, 491
MluCI AATT 6 cut(s) 160, 269, 411, 419, 464, 638
MmeI TCCRAC 2 cut(s) 607, 695
MnlI CCTC 7 cut(s) 97, 224, 388, 502, 592, 680, 760
MroXI GAANNNNTTC 2 cut(s) 359, 402
MseI TTAA 7 cut(s) 225, 422, 552, 615, 641, 735, 783
MslI CAYNNNNRTG 1 cut(s) 794
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 2 cut(s) 473, 772
MssI GTTTAAAC 1 cut(s) 616
Mva1269I GAATGC 2 cut(s) 349, 789
MvaI CCWGG 2 cut(s) 473, 772
MwoI GCNNNNNNNGC 1 cut(s) 314
NdeII GATC 2 cut(s) 79, 828
NlaIII CATG 4 cut(s) 457, 596, 612, 733
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 2 cut(s) 23, 288
NspI RCATGY 1 cut(s) 596
PagI TCATGA 1 cut(s) 729
PceI AGGCCT 1 cut(s) 770
PctI GAATGC 2 cut(s) 349, 789
PdmI GAANNNNTTC 2 cut(s) 359, 402
PfeI GAWTC 1 cut(s) 516
PflFI GACNNNGTC 1 cut(s) 664
PkrI GCNGC 3 cut(s) 214, 265, 538
PmeI GTTTAAAC 1 cut(s) 616
PshBI ATTAAT 1 cut(s) 735
PsiI TTATAA 3 cut(s) 165, 434, 695
Psp6I CCWGG 2 cut(s) 471, 770
PspGI CCWGG 2 cut(s) 471, 770
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 195, 660
PsyI GACNNNGTC 1 cut(s) 664
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
RseI CAYNNNNRTG 1 cut(s) 794
SaqAI TTAA 7 cut(s) 225, 422, 552, 615, 641, 735, 783
SatI GCNGC 3 cut(s) 213, 264, 537
Sau3AI GATC 2 cut(s) 79, 828
Sau96I GGNCC 2 cut(s) 195, 660
ScrFI CCNGG 2 cut(s) 473, 772
SetI ASST 9 cut(s) 35, 44, 100, 135, 310, 440, 622, 682, 766
SfaNI GCATC 2 cut(s) 73, 482
SinI GGWCC 2 cut(s) 195, 660
SmiMI CAYNNNNRTG 1 cut(s) 794
Sse9I AATT 6 cut(s) 160, 269, 411, 419, 464, 638
SseBI AGGCCT 1 cut(s) 770
SspI AATATT 2 cut(s) 502, 739
SspMI CTAG 2 cut(s) 39, 141
StuI AGGCCT 1 cut(s) 770
StyD4I CCNGG 2 cut(s) 471, 770
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 2 cut(s) 56, 486
TaiI ACGT 1 cut(s) 35
TasI AATT 6 cut(s) 160, 269, 411, 419, 464, 638
TfiI GAWTC 1 cut(s) 516
Tru1I TTAA 7 cut(s) 225, 422, 552, 615, 641, 735, 783
Tru9I TTAA 7 cut(s) 225, 422, 552, 615, 641, 735, 783
TscAI CASTG 1 cut(s) 14
TseFI GTSAC 2 cut(s) 23, 288
TseI GCWGC 3 cut(s) 212, 263, 536
Tsp45I GTSAC 2 cut(s) 23, 288
TspDTI ATGAA 2 cut(s) 137, 718
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 1 cut(s) 14
Tth111I GACNNNGTC 1 cut(s) 664
VpaK11BI GGWCC 2 cut(s) 195, 660
VspI ATTAAT 1 cut(s) 735
XapI RAATTY 2 cut(s) 269, 411
XceI RCATGY 1 cut(s) 596
XmaJI CCTAGG 1 cut(s) 38
XmnI GAANNNNTTC 2 cut(s) 359, 402
XspI CTAG 2 cut(s) 39, 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.