Rmu_ssc0000158.1_g000033

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000158.1
OG0000254
Physical Location & Seq
Forward (+)
150971 .. 152303
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000158.1_g000033.1.cds

Sequence Viewer

Length: 1056 bp
atgtggagaattggcaatggtgctagtaccaagttttgggttgacagatggacttcctgtggtatccttgaaaattatgctctgaatcatgatatcatcgatattaattctcttgtgcaagatttttggattaataatgattgtaatttgcctatgttacttgctaatctccctgctgacattgtggataaaatcactgctatccctttagcttatagtgatttacctgataagctaatttggggaagtacctcttctagcattttctctgttaaatctgcttacaagcttttgtgtaaaccactgactaatgagcaaagagtgatcaaaaggcttgcaaccaactcttcttgccagctctgtgttaacagtcctgagtctatgttacacatatttcgggactgccctaaagcgaaacaggtatggcaatgttttaatatccctccaaatatgttgactacatttagtttatgctggaatgattggattttggccaatctcctacagaaaggctattatatgaggaacctgaactggaacatcttctttatcttttgttgttggttcttatggaaatggagatgtaaatgcgtctttgatcgtaacttctgctatcctcacaatcttactaatgtagtgtttaactatggggatgagtggactagggccagtgataaggctagtttgaagaaaagaagccatgttgagctgctttcttggattaagcctacggttggagtccataagcttaatgttgatgggtctaaaacaaccaatggttctattagagcagggggtgttataagggacaatactggtagctggtgtggtggttttatgattaatattggtgttggtgaagtcctccaagctgaagtcaagcctatccatcgtgagcggaattcagttgttcatctcttagctaagaacagcattcttcatgctaaaggggtctgcaccctccatgatcctcctgctttagtcaacgaagctctcctggatgatattgttggagctcctagagctagaaattttagtgccagcaatgctagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

351

Amino Acids

39.8

Weight (kDa)

8.85

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 803
AccB7I CCANNNNNTGG 1 cut(s) 36
AccBSI CCGCTC 1 cut(s) 898
AciI CCGC 1 cut(s) 898
AclWI GGATC 1 cut(s) 962
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 2 cut(s) 901, 1030
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 2 cut(s) 28, 250
AfiI CCNNNNNNNGG 2 cut(s) 36, 734
AgsI TTSAA 2 cut(s) 71, 688
AjnI CCWGG 1 cut(s) 996
Alw21I GWGCWC 1 cut(s) 1018
AlwI GGATC 1 cut(s) 962
AoxI GGCC 2 cut(s) 492, 666
ApeKI GCWGC 1 cut(s) 709
ApoI RAATTY 2 cut(s) 901, 1030
AseI ATTAAT 3 cut(s) 105, 132, 843
Asp700I GAANNNNTTC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 666
AsuHPI GGTGA 1 cut(s) 869
AsuNHI GCTAGC 1 cut(s) 1049
BalI TGGCCA 1 cut(s) 494
BanII GRGCYC 1 cut(s) 1018
Bbv12I GWGCWC 1 cut(s) 1018
BbvI GCAGC 1 cut(s) 696
BccI CCATC 3 cut(s) 42, 752, 897
BciT130I CCWGG 1 cut(s) 998
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 324
BfaI CTAG 8 cut(s) 24, 258, 663, 681, 1020, 1026, 1050, 1054
BfmI CTRYAG 1 cut(s) 503
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 1 cut(s) 710
BlsI GCNGC 1 cut(s) 711
Bme1390I CCNGG 1 cut(s) 998
BmgT120I GGNCC 1 cut(s) 666
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 1 cut(s) 998
BmtI GCTAGC 1 cut(s) 1053
Bsa29I ATCGAT 1 cut(s) 99
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 734
Bse1I ACTGG 3 cut(s) 539, 669, 820
Bse3DI GCAATG 3 cut(s) 22, 434, 1051
BseBI CCWGG 1 cut(s) 998
BseCI ATCGAT 1 cut(s) 99
BseGI GGATG 2 cut(s) 658, 1006
BseLI CCNNNNNNNGG 2 cut(s) 36, 734
BseMI GCAATG 3 cut(s) 22, 434, 1051
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 3 cut(s) 539, 669, 820
BseXI GCAGC 1 cut(s) 696
BsgI GTGCAG 1 cut(s) 940
BshFI GGCC 2 cut(s) 494, 668
BshVI ATCGAT 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 1018
BslFI GGGAC 2 cut(s) 413, 821
BslI CCNNNNNNNGG 2 cut(s) 36, 734
BsmFI GGGAC 2 cut(s) 413, 821
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 2 cut(s) 494, 668
Bsp1286I GDGCHC 1 cut(s) 1018
Bsp143I GATC 3 cut(s) 324, 598, 967
BspACI CCGC 1 cut(s) 898
BspANI GGCC 2 cut(s) 494, 668
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 99
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 527
BspOI GCTAGC 1 cut(s) 1053
BspPI GGATC 1 cut(s) 962
BsrBI CCGCTC 1 cut(s) 898
BsrDI GCAATG 3 cut(s) 22, 434, 1051
BsrI ACTGG 3 cut(s) 539, 669, 820
BssMI GATC 3 cut(s) 324, 598, 967
Bst2UI CCWGG 1 cut(s) 998
Bst4CI ACNGT 2 cut(s) 371, 733
Bst6I CTCTTC 2 cut(s) 259, 352
BstC8I GCNNGC 4 cut(s) 336, 356, 1042, 1051
BstDEI CTNAG 3 cut(s) 375, 919, 924
BstF5I GGATG 2 cut(s) 658, 1006
BstKTI GATC 3 cut(s) 327, 601, 970
BstMBI GATC 3 cut(s) 324, 598, 967
BstMWI GCNNNNNNNGC 3 cut(s) 1022, 1046, 1050
BstNI CCWGG 1 cut(s) 998
BstSCI CCNGG 1 cut(s) 996
BstSFI CTRYAG 1 cut(s) 503
BstV1I GCAGC 1 cut(s) 696
Bsu15I ATCGAT 1 cut(s) 99
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 2 cut(s) 494, 668
BsuTUI ATCGAT 1 cut(s) 99
BtsCI GGATG 2 cut(s) 658, 1006
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 3 cut(s) 195, 302, 676
Cac8I GCNNGC 4 cut(s) 336, 356, 1042, 1051
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 666
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 1 cut(s) 580
Csp6I GTAC 2 cut(s) 27, 249
CviAII CATG 4 cut(s) 89, 701, 941, 965
CviQI GTAC 2 cut(s) 27, 249
DdeI CTNAG 3 cut(s) 375, 919, 924
DpnI GATC 3 cut(s) 326, 600, 969
DpnII GATC 3 cut(s) 324, 598, 967
EaeI YGGCCR 1 cut(s) 492
Eam1104I CTCTTC 2 cut(s) 259, 352
EarI CTCTTC 2 cut(s) 259, 352
Ecl136II GAGCTC 1 cut(s) 1016
Eco24I GRGCYC 1 cut(s) 1018
Eco32I GATATC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 1 cut(s) 894
EcoICRI GAGCTC 1 cut(s) 1016
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 1 cut(s) 996
EcoRV GATATC 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 1018
FaeI CATG 4 cut(s) 92, 704, 944, 968
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 413, 821
FatI CATG 4 cut(s) 88, 700, 940, 964
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 710
FokI GGATG 2 cut(s) 665, 1013
FriOI GRGCYC 1 cut(s) 1018
Fsp4HI GCNGC 1 cut(s) 710
FspBI CTAG 8 cut(s) 24, 258, 663, 681, 1020, 1026, 1050, 1054
GluI GCNGC 1 cut(s) 710
HaeIII GGCC 2 cut(s) 494, 668
HgaI GACGC 1 cut(s) 580
Hin1II CATG 4 cut(s) 92, 704, 944, 968
HincII GTYRAC 4 cut(s) 43, 367, 456, 985
HindII GTYRAC 4 cut(s) 43, 367, 456, 985
HindIII AAGCTT 2 cut(s) 287, 746
HinfI GANTC 3 cut(s) 85, 377, 738
HpaI GTTAAC 1 cut(s) 367
HphI GGTGA 1 cut(s) 869
Hpy166II GTNNAC 6 cut(s) 43, 299, 367, 456, 660, 985
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 4 cut(s) 89, 374, 398, 893
Hpy8I GTNNAC 6 cut(s) 43, 299, 367, 456, 660, 985
HpyCH4III ACNGT 2 cut(s) 371, 733
HpyCH4V TGCA 3 cut(s) 118, 338, 957
HpyF10VI GCNNNNNNNGC 3 cut(s) 1022, 1046, 1050
HpyF3I CTNAG 3 cut(s) 375, 919, 924
Hsp92II CATG 4 cut(s) 92, 704, 944, 968
Ksp22I TGATCA 1 cut(s) 324
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 3 cut(s) 324, 598, 967
LmnI GCTCC 2 cut(s) 1013, 1021
Lsp1109I GCAGC 1 cut(s) 696
MaeI CTAG 8 cut(s) 24, 258, 663, 681, 1020, 1026, 1050, 1054
MaeIII GTNAC 3 cut(s) 156, 384, 602
MalI GATC 3 cut(s) 326, 600, 969
MbiI CCGCTC 1 cut(s) 898
MboI GATC 3 cut(s) 324, 598, 967
MboII GAAGA 5 cut(s) 246, 339, 535, 700, 929
MhlI GDGCHC 1 cut(s) 1018
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 7 cut(s) 9, 73, 106, 145, 237, 901, 1030
MluNI TGGCCA 1 cut(s) 494
MlyI GAGTC 2 cut(s) 386, 747
MmeI TCCRAC 2 cut(s) 715, 991
MnlI CCTC 7 cut(s) 262, 453, 516, 627, 875, 971, 981
Mox20I TGGCCA 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 542
MscI TGGCCA 1 cut(s) 494
MseI TTAA 9 cut(s) 105, 132, 273, 366, 435, 642, 723, 750, 843
Msp20I TGGCCA 1 cut(s) 494
MspR9I CCNGG 1 cut(s) 998
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 1 cut(s) 998
MwoI GCNNNNNNNGC 3 cut(s) 1022, 1046, 1050
NdeII GATC 3 cut(s) 324, 598, 967
NheI GCTAGC 1 cut(s) 1049
NlaIII CATG 4 cut(s) 92, 704, 944, 968
NlaIV GGNNCC 1 cut(s) 527
PagI TCATGA 1 cut(s) 88
PctI GAATGC 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 542
PfeI GAWTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 36
PfoI TCCNGGA 1 cut(s) 996
PkrI GCNGC 1 cut(s) 711
PleI GAGTC 2 cut(s) 385, 746
PpsI GAGTC 2 cut(s) 385, 746
PshBI ATTAAT 3 cut(s) 105, 132, 843
PsiI TTATAA 1 cut(s) 803
Psp124BI GAGCTC 1 cut(s) 1018
Psp6I CCWGG 1 cut(s) 996
PspGI CCWGG 1 cut(s) 996
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 1 cut(s) 666
RsaI GTAC 2 cut(s) 28, 250
RsaNI GTAC 2 cut(s) 27, 249
SacI GAGCTC 1 cut(s) 1018
SaqAI TTAA 9 cut(s) 105, 132, 273, 366, 435, 642, 723, 750, 843
SatI GCNGC 1 cut(s) 710
Sau3AI GATC 3 cut(s) 324, 598, 967
Sau96I GGNCC 1 cut(s) 666
SchI GAGTC 2 cut(s) 386, 747
ScrFI CCNGG 1 cut(s) 998
SduI GDGCHC 1 cut(s) 1018
SfcI CTRYAG 1 cut(s) 503
Sse9I AATT 7 cut(s) 9, 73, 106, 145, 237, 901, 1030
SsiI CCGC 1 cut(s) 898
SspI AATATT 1 cut(s) 847
SspMI CTAG 8 cut(s) 24, 258, 663, 681, 1020, 1026, 1050, 1054
SstI GAGCTC 1 cut(s) 1018
StyD4I CCNGG 1 cut(s) 996
TaaI ACNGT 2 cut(s) 371, 733
TaqI TCGA 1 cut(s) 99
TasI AATT 7 cut(s) 9, 73, 106, 145, 237, 901, 1030
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 9 cut(s) 105, 132, 273, 366, 435, 642, 723, 750, 843
Tru9I TTAA 9 cut(s) 105, 132, 273, 366, 435, 642, 723, 750, 843
TscAI CASTG 3 cut(s) 202, 309, 676
TseI GCWGC 1 cut(s) 709
TspDTI ATGAA 2 cut(s) 902, 929
TspRI CASTG 3 cut(s) 202, 309, 676
Van91I CCANNNNNTGG 1 cut(s) 36
VspI ATTAAT 3 cut(s) 105, 132, 843
XapI RAATTY 2 cut(s) 901, 1030
XmnI GAANNNNTTC 1 cut(s) 542
XspI CTAG 8 cut(s) 24, 258, 663, 681, 1020, 1026, 1050, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.