Rorug05G0070500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000254
Physical Location & Seq
Reverse (-)
6021596 .. 6023921
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0070500.1

Sequence Viewer

Length: 654 bp
ATGGGAGCGTACAGAGCTGATGATGATTACGATTACTTGTTCAAGGTGGTGTTGATCGGCGACTCTGGGGTTGGCAAATCGAACCTCTTGTCCAGGTTCACCAGGAATGAGTTCAGCCTTGAATCAAAGTCCACCATTGGGGTTGAGTTTGCCACCAGAAGCATTAGGGTCGATGATAAGGTGGTTAAGGCCCAGATTTGGGACACTGCTGGTCAAGAAAGGTATCGTGCAATCACAAGTGCATATTATCGAGGTGCAGTCGGTGCATTACTAGTCTATGATGTTACTCGTCATGTTACATTTGAAAATGTGGAAAGATGGTTGAAGGAGCTTCGGGACCATACAGATGCCAACATTGTGATCATGTTGGTGGGAAACAAGGGAGATCTGCGTCACCTGCGAGCCGTTTCCACTGAGGATGCCCAGGCTTTTGCTGAACGAGAGAATACCTTTTTCATGGAAACATCTGCCCTTGAGTCCATGAATGTTGACAATGCTTTCACAGAAGTGCTGAGCCAAATCTACCGTGTTGTAAGCAGGAAGGCCCTAGACATTGGGGATGATCCAGCAGCCTTGCCCAAGGGACAGACGATCAATGTTGGTGGCAAGGATGATGTATCAGCTGTGAAGAAAGTTGGATGTTGCTCCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

217

Amino Acids

24.15

Weight (kDa)

5.49

Isoelectric Point (pI)

21.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 168 3.5e-12 ADP-ribosylation factor family
Ras PF00071 15 - 175 5.4e-64 Ras family
Roc PF08477 15 - 129 6.8e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 121 7.2e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 405
AclWI GGATC 1 cut(s) 557
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 3 cut(s) 138, 198, 199
AgsI TTSAA 4 cut(s) 43, 122, 305, 325
AhlI ACTAGT 1 cut(s) 271
AjnI CCWGG 3 cut(s) 92, 101, 423
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 3 cut(s) 17, 331, 623
AluI AGCT 3 cut(s) 17, 331, 623
AlwI GGATC 1 cut(s) 557
AoxI GGCC 2 cut(s) 189, 543
ApeKI GCWGC 1 cut(s) 569
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 1 cut(s) 110
AspS9I GGNCC 3 cut(s) 190, 337, 544
AsuHPI GGTGA 2 cut(s) 91, 386
AvaII GGWCC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 581
BccI CCATC 1 cut(s) 312
BceAI ACGGC 1 cut(s) 389
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 3 cut(s) 94, 103, 425
BclI TGATCA 1 cut(s) 360
BcuI ACTAGT 1 cut(s) 271
BfaI CTAG 2 cut(s) 272, 548
BfuAI ACCTGC 1 cut(s) 405
BglII AGATCT 1 cut(s) 385
BisI GCNGC 1 cut(s) 570
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 3 cut(s) 94, 103, 425
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 3 cut(s) 190, 337, 544
BmiI GGNNCC 1 cut(s) 338
BmrFI CCNGG 3 cut(s) 94, 103, 425
BmsI GCATC 2 cut(s) 337, 409
Bpu1102I GCTNAGC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 494
BsaJI CCNNGG 2 cut(s) 423, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 198, 199
BseBI CCWGG 3 cut(s) 94, 103, 425
BseDI CCNNGG 2 cut(s) 423, 579
BseGI GGATG 4 cut(s) 424, 565, 616, 644
BseLI CCNNNNNNNGG 3 cut(s) 138, 198, 199
BseMII CTCAG 2 cut(s) 405, 503
BseXI GCAGC 1 cut(s) 581
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 2 cut(s) 191, 545
BslFI GGGAC 3 cut(s) 215, 350, 597
BslI CCNNNNNNNGG 3 cut(s) 138, 198, 199
BsmFI GGGAC 3 cut(s) 215, 350, 597
BsnI GGCC 2 cut(s) 191, 545
Bsp143I GATC 5 cut(s) 54, 360, 385, 562, 591
Bsp1720I GCTNAGC 1 cut(s) 512
BspANI GGCC 2 cut(s) 191, 545
BspCNI CTCAG 2 cut(s) 406, 504
BspLI GGNNCC 1 cut(s) 338
BspMI ACCTGC 1 cut(s) 405
BspPI GGATC 1 cut(s) 557
BssECI CCNNGG 2 cut(s) 423, 579
BssMI GATC 5 cut(s) 54, 360, 385, 562, 591
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 94, 103, 425
Bst4CI ACNGT 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 2 cut(s) 414, 512
BstF5I GGATG 4 cut(s) 424, 565, 616, 644
BstKTI GATC 5 cut(s) 57, 363, 388, 565, 594
BstMBI GATC 5 cut(s) 54, 360, 385, 562, 591
BstMWI GCNNNNNNNGC 3 cut(s) 14, 263, 397
BstNI CCWGG 3 cut(s) 94, 103, 425
BstSCI CCNGG 3 cut(s) 92, 101, 423
BstV1I GCAGC 1 cut(s) 581
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 2 cut(s) 191, 545
BtsCI GGATG 4 cut(s) 424, 565, 616, 644
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 2 cut(s) 204, 411
BveI ACCTGC 1 cut(s) 405
Cac8I GCNNGC 1 cut(s) 402
Cfr13I GGNCC 3 cut(s) 190, 337, 544
CseI GACGC 1 cut(s) 380
Csp6I GTAC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 583, 618
CviAII CATG 4 cut(s) 293, 364, 457, 481
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 414, 512
DpnI GATC 5 cut(s) 56, 362, 387, 564, 593
DpnII GATC 5 cut(s) 54, 360, 385, 562, 591
Eco130I CCWWGG 1 cut(s) 579
Eco47I GGWCC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 544
EcoRII CCWGG 3 cut(s) 92, 101, 423
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 4 cut(s) 296, 367, 460, 484
FaiI YATR 7 cut(s) 244, 279, 294, 342, 365, 458, 482
FaqI GGGAC 3 cut(s) 215, 350, 597
FatI CATG 4 cut(s) 292, 363, 456, 480
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 570
FokI GGATG 3 cut(s) 431, 572, 623
Fsp4HI GCNGC 1 cut(s) 570
FspBI CTAG 2 cut(s) 272, 548
GluI GCNGC 1 cut(s) 570
HaeIII GGCC 2 cut(s) 191, 545
HgaI GACGC 1 cut(s) 380
Hin1II CATG 4 cut(s) 296, 367, 460, 484
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 3 cut(s) 62, 122, 476
HphI GGTGA 2 cut(s) 91, 386
Hpy166II GTNNAC 3 cut(s) 99, 132, 490
Hpy188III TCNNGA 2 cut(s) 215, 335
Hpy8I GTNNAC 3 cut(s) 99, 132, 490
HpyAV CCTTC 2 cut(s) 319, 535
HpyCH4III ACNGT 1 cut(s) 527
HpyCH4V TGCA 4 cut(s) 230, 242, 257, 266
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 263, 397
HpyF3I CTNAG 2 cut(s) 414, 512
Hsp92II CATG 4 cut(s) 296, 367, 460, 484
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 5 cut(s) 54, 360, 385, 562, 591
LmnI GCTCC 3 cut(s) 5, 328, 650
Lsp1109I GCAGC 1 cut(s) 581
LweI GCATC 2 cut(s) 337, 409
MaeI CTAG 2 cut(s) 272, 548
MaeIII GTNAC 3 cut(s) 283, 295, 392
MalI GATC 5 cut(s) 56, 362, 387, 564, 593
MboI GATC 5 cut(s) 54, 360, 385, 562, 591
MboII GAAGA 1 cut(s) 640
MflI RGATCY 1 cut(s) 385
MlyI GAGTC 2 cut(s) 56, 485
MmeI TCCRAC 1 cut(s) 616
MnlI CCTC 3 cut(s) 95, 245, 409
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 2 cut(s) 186, 652
MslI CAYNNNNRTG 3 cut(s) 345, 368, 506
MspA1I CMGCKG 1 cut(s) 623
MspR9I CCNGG 3 cut(s) 94, 103, 425
MvaI CCWGG 3 cut(s) 94, 103, 425
MwoI GCNNNNNNNGC 3 cut(s) 14, 263, 397
NdeII GATC 5 cut(s) 54, 360, 385, 562, 591
NlaIII CATG 4 cut(s) 296, 367, 460, 484
NlaIV GGNNCC 1 cut(s) 338
NmuCI GTSAC 1 cut(s) 392
OliI CACNNNNGTG 1 cut(s) 506
PaqCI CACCTGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 110
PfeI GAWTC 1 cut(s) 122
PkrI GCNGC 1 cut(s) 571
PleI GAGTC 2 cut(s) 56, 484
PpsI GAGTC 2 cut(s) 56, 484
Psp6I CCWGG 3 cut(s) 92, 101, 423
PspGI CCWGG 3 cut(s) 92, 101, 423
PspN4I GGNNCC 1 cut(s) 338
PspPI GGNCC 3 cut(s) 190, 337, 544
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 3 cut(s) 345, 368, 506
SaqAI TTAA 2 cut(s) 186, 652
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 5 cut(s) 54, 360, 385, 562, 591
Sau96I GGNCC 3 cut(s) 190, 337, 544
SchI GAGTC 2 cut(s) 56, 485
ScrFI CCNGG 3 cut(s) 94, 103, 425
SfaNI GCATC 2 cut(s) 337, 409
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 3 cut(s) 345, 368, 506
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SpeI ACTAGT 1 cut(s) 271
SspMI CTAG 2 cut(s) 272, 548
StyD4I CCNGG 3 cut(s) 92, 101, 423
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 1 cut(s) 527
TaqI TCGA 3 cut(s) 80, 171, 250
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 2 cut(s) 186, 652
Tru9I TTAA 2 cut(s) 186, 652
TscAI CASTG 2 cut(s) 211, 418
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 2 cut(s) 445, 497
TspRI CASTG 2 cut(s) 211, 418
VpaK11BI GGWCC 1 cut(s) 337
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 2 cut(s) 272, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.