FvH4_2g06731

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000254
Physical Location & Seq
Forward (+)
5582144 .. 5585787
3644 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06731.t1

Sequence Viewer

Length: 1002 bp
ATGTCCGACAACGCTTCTAGGACATTGTCGTCGAATAGGAGAATGGAGGGTGGCCATGGGGTTGCTCTCGCACGGGGTTGGCTGTCGATCAAGCGTGGTCGTTTCGATCTTTTTGACAGGATGATGATGAGATCGGATGTTGGCAGTCATTGGTGGCAAGGCCGTGGACCTGCGCTCAGATGCACTGTTCCTACCCTTATTAACTCTGCTCTCCCTTCTGCTGTTATTTTTAAAGATGCTGTGATTAGTAATTTTTGGAATGCTGAAGGTTGGGATGTGGAGCTTTTGTTGTCTGTGCTCCCTAGAGAAATTGTTAATCTTATTGTTAATGTGCCATCTGGTTTTAGTGAGAGTGGTGATGATACTAGAATTTGGTGTTCTACTTCTAATGGCCAATTTTCAGTGAATTTATATGGAAGTGGAGAAACAAGCAAGTTTTTGAACCTGGTTTTTCCTACCGGTTTCAAAAAGATCATCTGGGACTATGCTTTGGAATGGAGAAAGTCTAAACTGAAATCCAATGACAATTACATGTTCAACTATACTATGCATGCTTGGAAAAAGCCTCCTGAAAATTTTTTCAAGCTCAATGTTGATGGAACTAGAGTATCTCCTATTGGTAAAATTGGTGTTGGTGGTGTCATTCGGAATCATTATGGTGACCTGAAGCTTGCTGTTAGCTTGAATATCAAGAACCTTATCATTGAGTCTGATTCTGCAATCTTAGTCCAGCTGATGAATAACTCTGAGTTTGGCAATCATCCTTTGGGATCTCTTCTTCAGGGATGCAGTAGCTTGATGAACAAGATGGAGAATGCCACCTTATCTCATATCTTCAGAGAATGCAACTCTACTGCTGATGCCTTAGCTAAATGCAGCATTTCTCATGAGCTTGGCCTTGTCTGTTTTGACAGCCCTCCTGCTCATGTTGCTGATGCATTCTTAGATGACTTGTGCGCTGTTTCTAAAGCCAAGAGAGCTGGCAACTGTTTTAGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

334

Amino Acids

36.86

Weight (kDa)

8.58

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 222 - 291 1.5e-14 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 178
AclWI GGATC 1 cut(s) 778
AcoI YGGCCR 2 cut(s) 52, 391
AcsI RAATTY 3 cut(s) 369, 406, 574
AcuI CTGAAG 4 cut(s) 285, 686, 764, 820
AflIII ACRYGT 1 cut(s) 531
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 5 cut(s) 442, 466, 538, 583, 685
AjnI CCWGG 1 cut(s) 444
AluBI AGCT 9 cut(s) 283, 586, 670, 681, 733, 795, 869, 892, 980
AluI AGCT 9 cut(s) 283, 586, 670, 681, 733, 795, 869, 892, 980
Alw21I GWGCWC 1 cut(s) 300
AlwI GGATC 1 cut(s) 778
AoxI GGCC 4 cut(s) 52, 160, 391, 895
ApeKI GCWGC 1 cut(s) 876
ApoI RAATTY 3 cut(s) 369, 406, 574
AsiGI ACCGGT 1 cut(s) 458
AspLEI GCGC 2 cut(s) 175, 959
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 368, 671
AvaII GGWCC 1 cut(s) 167
BaeI ACNNNNGTAYC 2 cut(s) 591, 624
BalI TGGCCA 2 cut(s) 54, 393
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 888
BccI CCATC 3 cut(s) 343, 590, 802
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 1 cut(s) 446
BfaI CTAG 5 cut(s) 18, 303, 366, 603, 1000
BfuAI ACCTGC 1 cut(s) 178
BisI GCNGC 1 cut(s) 877
BlsI GCNGC 1 cut(s) 878
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 5 cut(s) 170, 226, 776, 850, 925
Bpu10I CCTNAGC 1 cut(s) 865
BsaJI CCNNGG 2 cut(s) 55, 163
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bse118I RCCGGY 1 cut(s) 458
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 2 cut(s) 55, 163
BseGI GGATG 5 cut(s) 126, 142, 280, 760, 791
BseMII CTCAG 2 cut(s) 190, 738
BseXI GCAGC 1 cut(s) 888
BshFI GGCC 4 cut(s) 54, 162, 393, 897
BshTI ACCGGT 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 4 cut(s) 265, 820, 848, 938
BsnI GGCC 4 cut(s) 54, 162, 393, 897
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 5 cut(s) 87, 106, 131, 471, 770
Bsp19I CCATGG 1 cut(s) 55
BspANI GGCC 4 cut(s) 54, 162, 393, 897
BspCNI CTCAG 2 cut(s) 189, 739
BspHI TCATGA 1 cut(s) 886
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 1 cut(s) 778
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 55, 163
BssMI GATC 5 cut(s) 87, 106, 131, 471, 770
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 2 cut(s) 187, 989
Bst6I CTCTTC 1 cut(s) 780
BstC8I GCNNGC 3 cut(s) 552, 672, 982
BstDEI CTNAG 5 cut(s) 176, 724, 747, 865, 943
BstDSI CCRYGG 2 cut(s) 55, 163
BstEII GGTNACC 1 cut(s) 659
BstF5I GGATG 5 cut(s) 126, 142, 280, 760, 791
BstHHI GCGC 2 cut(s) 175, 959
BstKTI GATC 5 cut(s) 90, 109, 134, 474, 773
BstMBI GATC 5 cut(s) 87, 106, 131, 471, 770
BstMWI GCNNNNNNNGC 2 cut(s) 929, 977
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 2 cut(s) 535, 554
BstPI GGTNACC 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 444
BstV1I GCAGC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 770
BstYI RGATCY 1 cut(s) 770
BsuRI GGCC 4 cut(s) 54, 162, 393, 897
BtgI CCRYGG 2 cut(s) 55, 163
BtsCI GGATG 5 cut(s) 126, 142, 280, 760, 791
BtsIMutI CAGTG 2 cut(s) 183, 408
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 3 cut(s) 552, 672, 982
CciI TCATGA 1 cut(s) 886
CfoI GCGC 2 cut(s) 175, 959
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 444
CspAI ACCGGT 1 cut(s) 458
CviAII CATG 5 cut(s) 56, 532, 551, 887, 926
DdeI CTNAG 5 cut(s) 176, 724, 747, 865, 943
DpnI GATC 5 cut(s) 89, 108, 133, 473, 772
DpnII GATC 5 cut(s) 87, 106, 131, 471, 770
DraI TTTAAA 1 cut(s) 232
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 52, 391
Eam1104I CTCTTC 1 cut(s) 780
EarI CTCTTC 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 167
Eco57I CTGAAG 4 cut(s) 285, 686, 764, 820
Eco91I GGTNACC 1 cut(s) 659
EcoO65I GGTNACC 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 55
EcoT22I ATGCAT 2 cut(s) 552, 940
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 5 cut(s) 59, 535, 554, 890, 929
FaqI GGGAC 1 cut(s) 494
FatI CATG 5 cut(s) 55, 531, 550, 886, 925
Fnu4HI GCNGC 1 cut(s) 877
FokI GGATG 5 cut(s) 133, 149, 287, 747, 798
Fsp4HI GCNGC 1 cut(s) 877
FspBI CTAG 5 cut(s) 18, 303, 366, 603, 1000
GlaI GCGC 2 cut(s) 174, 958
GluI GCNGC 1 cut(s) 877
HaeIII GGCC 4 cut(s) 54, 162, 393, 897
HapII CCGG 1 cut(s) 459
HhaI GCGC 2 cut(s) 175, 959
Hin1II CATG 5 cut(s) 59, 535, 554, 890, 929
Hin6I GCGC 2 cut(s) 173, 957
HinP1I GCGC 2 cut(s) 173, 957
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 3 cut(s) 649, 707, 713
HpaII CCGG 1 cut(s) 459
HphI GGTGA 2 cut(s) 368, 671
Hpy166II GTNNAC 1 cut(s) 167
Hpy188I TCNGA 7 cut(s) 7, 136, 179, 648, 712, 748, 839
Hpy188III TCNNGA 3 cut(s) 569, 691, 887
Hpy8I GTNNAC 1 cut(s) 167
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 2 cut(s) 225, 260
HpyCH4III ACNGT 2 cut(s) 187, 989
HpyCH4V TGCA 7 cut(s) 183, 550, 719, 789, 846, 876, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 929, 977
HpyF3I CTNAG 5 cut(s) 176, 724, 747, 865, 943
Hsp92II CATG 5 cut(s) 59, 535, 554, 890, 929
HspAI GCGC 2 cut(s) 173, 957
Kzo9I GATC 5 cut(s) 87, 106, 131, 471, 770
LmnI GCTCC 2 cut(s) 280, 303
Lsp1109I GCAGC 1 cut(s) 888
LweI GCATC 5 cut(s) 170, 226, 776, 850, 925
MabI ACCWGGT 1 cut(s) 444
MaeI CTAG 5 cut(s) 18, 303, 366, 603, 1000
MaeIII GTNAC 1 cut(s) 659
MalI GATC 5 cut(s) 89, 108, 133, 473, 772
MboI GATC 5 cut(s) 87, 106, 131, 471, 770
MboII GAAGA 3 cut(s) 767, 770, 826
MflI RGATCY 1 cut(s) 770
MhlI GDGCHC 1 cut(s) 300
MlsI TGGCCA 2 cut(s) 54, 393
MluCI AATT 8 cut(s) 250, 309, 369, 395, 406, 526, 574, 624
MluNI TGGCCA 2 cut(s) 54, 393
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 3 cut(s) 40, 576, 927
Mox20I TGGCCA 2 cut(s) 54, 393
Mph1103I ATGCAT 2 cut(s) 552, 940
MscI TGGCCA 2 cut(s) 54, 393
MseI TTAA 4 cut(s) 201, 231, 315, 327
MslI CAYNNNNRTG 1 cut(s) 657
Msp20I TGGCCA 2 cut(s) 54, 393
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 1 cut(s) 446
Mva1269I GAATGC 4 cut(s) 265, 820, 848, 938
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 2 cut(s) 929, 977
NcoI CCATGG 1 cut(s) 55
NdeII GATC 5 cut(s) 87, 106, 131, 471, 770
NlaIII CATG 5 cut(s) 59, 535, 554, 890, 929
NmuCI GTSAC 1 cut(s) 659
NsiI ATGCAT 2 cut(s) 552, 940
NspI RCATGY 2 cut(s) 535, 554
PaeI GCATGC 1 cut(s) 554
PagI TCATGA 1 cut(s) 886
PciI ACATGT 1 cut(s) 531
PctI GAATGC 4 cut(s) 265, 820, 848, 938
PfeI GAWTC 2 cut(s) 649, 713
PflFI GACNNNGTC 1 cut(s) 25
PinAI ACCGGT 1 cut(s) 458
PkrI GCNGC 1 cut(s) 878
PleI GAGTC 1 cut(s) 715
PpsI GAGTC 1 cut(s) 715
PscI ACATGT 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 444
PspEI GGTNACC 1 cut(s) 659
PspGI CCWGG 1 cut(s) 444
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 1 cut(s) 770
PsyI GACNNNGTC 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 657
SaqAI TTAA 4 cut(s) 201, 231, 315, 327
SatI GCNGC 1 cut(s) 877
Sau3AI GATC 5 cut(s) 87, 106, 131, 471, 770
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 1 cut(s) 716
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 1 cut(s) 300
SexAI ACCWGGT 1 cut(s) 444
SfaNI GCATC 5 cut(s) 170, 226, 776, 850, 925
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 657
SphI GCATGC 1 cut(s) 554
Sse9I AATT 8 cut(s) 250, 309, 369, 395, 406, 526, 574, 624
SspMI CTAG 5 cut(s) 18, 303, 366, 603, 1000
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 187, 989
TaqI TCGA 3 cut(s) 32, 86, 105
TasI AATT 8 cut(s) 250, 309, 369, 395, 406, 526, 574, 624
TfiI GAWTC 2 cut(s) 649, 713
Tru1I TTAA 4 cut(s) 201, 231, 315, 327
Tru9I TTAA 4 cut(s) 201, 231, 315, 327
TscAI CASTG 2 cut(s) 190, 408
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 1 cut(s) 876
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 2 cut(s) 752, 815
TspRI CASTG 2 cut(s) 190, 408
Tth111I GACNNNGTC 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 3 cut(s) 369, 406, 574
XceI RCATGY 2 cut(s) 535, 554
XspI CTAG 5 cut(s) 18, 303, 366, 603, 1000
Zsp2I ATGCAT 2 cut(s) 552, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.