Rmu_ssc0000217.1_g000028

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000217.1
OG0000254
Physical Location & Seq
Forward (+)
141477 .. 142844
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000217.1_g000028.1.cds

Sequence Viewer

Length: 720 bp
atgccgcagagactccatgattcgcagcaggcaagttgcgccctagagccaccccgcggcagcccacgcccacaatccgggagggacagatcgacatcgatccccccacacccagatcgaaccttctttctcccaccgtggaccatgcttcagctcaagagacccgctgcactcctaaaccaacatccactccggaaccggaatgcaacggcaagagcaccggccgggagaggttgcactagaacttttctcttctttctcgcgcgtgaggcgtgttcctcttttcttgtatttagtaattggattgatggctttcaaattaatcttggcattggggagatccttgatgctgaagcttggggtctcttctatgggctaaagttagcctctaagcataacattgctaaccttgagattgaatctgactctgctgtccttgttcagcttatgcaaaggtcaagcgcgcaattcacttcacccccctggttctatgcttgctgggtgtgtgttatattgatgtccaaactgcagaatgtcaagctgacacatatattcatggagtgtaatatgactgatgattcccttgccaagaacagcatcactcatggtcttggcctaatcacttttgacaacccccctgcgcatgctgcacaaactttccttgatgacttgtctgctgtgactagagctaggggaactggtttttgctctagttgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

239

Amino Acids

26.55

Weight (kDa)

8.69

Isoelectric Point (pI)

63.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 431
Acc16I TGCGCA 1 cut(s) 642
AccII CGCG 4 cut(s) 57, 263, 265, 464
AccIII TCCGGA 1 cut(s) 192
AciI CCGC 4 cut(s) 5, 55, 57, 165
AclWI GGATC 2 cut(s) 94, 334
AcoI YGGCCR 1 cut(s) 222
AcuI CTGAAG 2 cut(s) 134, 372
AfiI CCNNNNNNNGG 2 cut(s) 56, 77
AgsI TTSAA 2 cut(s) 317, 419
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 5 cut(s) 154, 356, 445, 541, 689
AluI AGCT 5 cut(s) 154, 356, 445, 541, 689
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 3 cut(s) 4, 154, 368
AlwI GGATC 2 cut(s) 94, 334
Aor13HI TCCGGA 1 cut(s) 192
AoxI GGCC 2 cut(s) 222, 613
ApeKI GCWGC 4 cut(s) 25, 60, 167, 647
AseI ATTAAT 1 cut(s) 321
AspLEI GCGC 5 cut(s) 41, 265, 464, 466, 643
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 2 cut(s) 79, 226
AsuHPI GGTGA 1 cut(s) 468
AvaII GGWCC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 4 cut(s) 37, 72, 154, 634
BccI CCATC 1 cut(s) 302
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 484
BcnI CCSGG 2 cut(s) 79, 226
BcoDI GTCTC 3 cut(s) 4, 154, 368
BfaI CTAG 5 cut(s) 44, 240, 684, 690, 711
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 6 cut(s) 5, 26, 58, 61, 168, 648
BlsI GCNGC 6 cut(s) 6, 27, 59, 62, 169, 649
Bme1390I CCNGG 3 cut(s) 79, 226, 484
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 3 cut(s) 79, 226, 484
BmsI GCATC 2 cut(s) 337, 606
BpuEI CTTGAG 2 cut(s) 140, 431
BpuMI CCSGG 2 cut(s) 79, 226
Bsa29I ATCGAT 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 94
BsaI GGTCTC 2 cut(s) 154, 368
BsaJI CCNNGG 3 cut(s) 55, 137, 482
BsaWI WCCGGW 2 cut(s) 192, 198
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 77
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 1 cut(s) 703
Bse3DI GCAATG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 192
BseBI CCWGG 1 cut(s) 484
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 3 cut(s) 55, 137, 482
BseGI GGATG 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 56, 77
BseMI GCAATG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 703
BsePI GCGCGC 1 cut(s) 462
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 4 cut(s) 37, 72, 154, 634
BseYI CCCAGC 1 cut(s) 498
BsgI GTGCAG 2 cut(s) 153, 633
Bsh1236I CGCG 4 cut(s) 57, 263, 265, 464
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 2 cut(s) 224, 615
BshVI ATCGAT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 5 cut(s) 78, 193, 199, 221, 225
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 2 cut(s) 56, 77
BsmAI GTCTC 3 cut(s) 4, 154, 368
BsmFI GGGAC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 2 cut(s) 224, 615
Bso31I GGTCTC 2 cut(s) 154, 368
Bsp1286I GDGCHC 1 cut(s) 220
Bsp13I TCCGGA 1 cut(s) 192
Bsp143I GATC 4 cut(s) 89, 99, 115, 339
BspACI CCGC 4 cut(s) 5, 55, 57, 165
BspANI GGCC 2 cut(s) 224, 615
BspDI ATCGAT 1 cut(s) 98
BspEI TCCGGA 1 cut(s) 192
BspFNI CGCG 4 cut(s) 57, 263, 265, 464
BspLI GGNNCC 1 cut(s) 197
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 2 cut(s) 94, 334
BspTNI GGTCTC 2 cut(s) 154, 368
BsrDI GCAATG 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 1 cut(s) 703
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 55, 137, 482
BssHII GCGCGC 1 cut(s) 462
BssMI GATC 4 cut(s) 89, 99, 115, 339
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 2 cut(s) 257, 371
BstC8I GCNNGC 4 cut(s) 30, 464, 496, 645
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 2 cut(s) 55, 137
BstF5I GGATG 1 cut(s) 184
BstFNI CGCG 4 cut(s) 57, 263, 265, 464
BstHHI GCGC 5 cut(s) 41, 265, 464, 466, 643
BstKTI GATC 4 cut(s) 92, 102, 118, 342
BstMAI GTCTC 3 cut(s) 4, 154, 368
BstMBI GATC 4 cut(s) 89, 99, 115, 339
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 4 cut(s) 38, 66, 269, 647
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 1 cut(s) 647
BstSCI CCNGG 3 cut(s) 77, 224, 482
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 4 cut(s) 57, 263, 265, 464
BstV1I GCAGC 4 cut(s) 37, 72, 154, 634
BstX2I RGATCY 1 cut(s) 339
BstYI RGATCY 1 cut(s) 339
BstZI CGGCCG 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 98
BsuRI GGCC 2 cut(s) 224, 615
BsuTUI ATCGAT 1 cut(s) 98
BtgI CCRYGG 2 cut(s) 55, 137
BtsCI GGATG 1 cut(s) 184
Cac8I GCNNGC 4 cut(s) 30, 464, 496, 645
CfoI GCGC 5 cut(s) 41, 265, 464, 466, 643
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 141
Cfr42I CCGCGG 1 cut(s) 58
ClaI ATCGAT 1 cut(s) 98
CviAII CATG 5 cut(s) 17, 145, 556, 605, 644
DdeI CTNAG 1 cut(s) 390
DpnI GATC 4 cut(s) 91, 101, 117, 341
DpnII GATC 4 cut(s) 89, 99, 115, 339
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 257, 371
EarI CTCTTC 2 cut(s) 257, 371
EclXI CGGCCG 1 cut(s) 222
Eco31I GGTCTC 2 cut(s) 154, 368
Eco47I GGWCC 1 cut(s) 141
Eco52I CGGCCG 1 cut(s) 222
Eco57I CTGAAG 2 cut(s) 134, 372
EcoRII CCWGG 1 cut(s) 482
FaeI CATG 5 cut(s) 20, 148, 559, 608, 647
FaqI GGGAC 1 cut(s) 98
FatI CATG 5 cut(s) 16, 144, 555, 604, 643
FauI CCCGC 2 cut(s) 62, 172
Fnu4HI GCNGC 6 cut(s) 5, 26, 58, 61, 168, 648
FokI GGATG 1 cut(s) 171
Fsp4HI GCNGC 6 cut(s) 5, 26, 58, 61, 168, 648
FspBI CTAG 5 cut(s) 44, 240, 684, 690, 711
FspI TGCGCA 1 cut(s) 642
GlaI GCGC 5 cut(s) 40, 264, 463, 465, 642
GluI GCNGC 6 cut(s) 5, 26, 58, 61, 168, 648
GsaI CCCAGC 1 cut(s) 502
HaeIII GGCC 2 cut(s) 224, 615
HapII CCGG 5 cut(s) 78, 193, 199, 221, 225
HhaI GCGC 5 cut(s) 41, 265, 464, 466, 643
Hin1II CATG 5 cut(s) 20, 148, 559, 608, 647
Hin6I GCGC 5 cut(s) 39, 263, 462, 464, 641
HinP1I GCGC 5 cut(s) 39, 263, 462, 464, 641
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 5 cut(s) 12, 20, 419, 425, 578
HpaII CCGG 5 cut(s) 78, 193, 199, 221, 225
HphI GGTGA 1 cut(s) 468
Hpy166II GTNNAC 1 cut(s) 141
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 2 cut(s) 157, 193
Hpy8I GTNNAC 1 cut(s) 141
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4V TGCA 6 cut(s) 170, 206, 237, 451, 529, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 66, 269, 647
HpyF3I CTNAG 1 cut(s) 390
Hsp92II CATG 5 cut(s) 20, 148, 559, 608, 647
HspAI GCGC 5 cut(s) 39, 263, 462, 464, 641
Kpn2I TCCGGA 1 cut(s) 192
KspI CCGCGG 1 cut(s) 58
Kzo9I GATC 4 cut(s) 89, 99, 115, 339
Lsp1109I GCAGC 4 cut(s) 37, 72, 154, 634
LweI GCATC 2 cut(s) 337, 606
MaeI CTAG 5 cut(s) 44, 240, 684, 690, 711
MaeIII GTNAC 1 cut(s) 679
MalI GATC 4 cut(s) 91, 101, 117, 341
MboI GATC 4 cut(s) 89, 99, 115, 339
MboII GAAGA 2 cut(s) 244, 358
MflI RGATCY 1 cut(s) 339
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 3 cut(s) 298, 318, 467
MlyI GAGTC 2 cut(s) 6, 419
MnlI CCTC 5 cut(s) 75, 224, 262, 289, 397
MroI TCCGGA 1 cut(s) 192
MseI TTAA 1 cut(s) 321
MspA1I CMGCKG 2 cut(s) 57, 167
MspI CCGG 5 cut(s) 78, 193, 199, 221, 225
MspR9I CCNGG 3 cut(s) 79, 226, 484
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 4 cut(s) 57, 263, 265, 464
MwoI GCNNNNNNNGC 4 cut(s) 38, 66, 269, 647
NciI CCSGG 2 cut(s) 79, 226
NdeII GATC 4 cut(s) 89, 99, 115, 339
NlaIII CATG 5 cut(s) 20, 148, 559, 608, 647
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 679
NsbI TGCGCA 1 cut(s) 642
NspI RCATGY 1 cut(s) 647
PaeI GCATGC 1 cut(s) 647
PauI GCGCGC 1 cut(s) 462
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 3 cut(s) 20, 419, 578
PfoI TCCNGGA 1 cut(s) 77
PkrI GCNGC 6 cut(s) 6, 27, 59, 62, 169, 649
PleI GAGTC 2 cut(s) 6, 419
PpsI GAGTC 2 cut(s) 6, 419
PshBI ATTAAT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 482
PspFI CCCAGC 1 cut(s) 498
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 141
PstI CTGCAG 1 cut(s) 531
PsuI RGATCY 1 cut(s) 339
PteI GCGCGC 1 cut(s) 462
SacII CCGCGG 1 cut(s) 58
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 6 cut(s) 5, 26, 58, 61, 168, 648
Sau3AI GATC 4 cut(s) 89, 99, 115, 339
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 2 cut(s) 6, 419
ScrFI CCNGG 3 cut(s) 79, 226, 484
SduI GDGCHC 1 cut(s) 220
SetI ASST 9 cut(s) 125, 156, 235, 358, 411, 447, 458, 543, 691
SfaNI GCATC 2 cut(s) 337, 606
SfcI CTRYAG 1 cut(s) 527
Sfr303I CCGCGG 1 cut(s) 58
SgrBI CCGCGG 1 cut(s) 58
SinI GGWCC 1 cut(s) 141
SmlI CTYRAG 2 cut(s) 155, 410
SmoI CTYRAG 2 cut(s) 155, 410
SphI GCATGC 1 cut(s) 647
Sse9I AATT 3 cut(s) 298, 318, 467
SsiI CCGC 4 cut(s) 5, 55, 57, 165
SspMI CTAG 5 cut(s) 44, 240, 684, 690, 711
StyD4I CCNGG 3 cut(s) 77, 224, 482
TaaI ACNGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 92, 98, 118
TasI AATT 3 cut(s) 298, 318, 467
TauI GCSGC 2 cut(s) 7, 60
TfiI GAWTC 3 cut(s) 20, 419, 578
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 4 cut(s) 25, 60, 167, 647
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 141
VspI ATTAAT 1 cut(s) 321
XceI RCATGY 1 cut(s) 647
XspI CTAG 5 cut(s) 44, 240, 684, 690, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.